检索结果-南通市图书馆

A mitochondria-targeted H_(2)S-activatable fluorogenic probe for tracking hepatic ischemia-reperfusion injury

Chinese Chemical Letters 2024年第6期35卷 370-375页

作者： Bin Fang Jiaqi Yang Limin Wang Haoqin Li Jiaying Guo Jiaxin Zhang Qingyuan Guo Bo Peng Kedi Liu miaomiao Xi Hua bai Li Fu Lin Li School of Materials Science and Engineering Northwestern Polytechnical UniversityXi’an 710072China Frontiers Science Center for Flexible Electronics Xi’an Institute of Flexible Electronics(IFE)and Xi’an Institute of Biomedical Materials&EngineeringNorthwestern Polytechnical UniversityXi’an 710072China Key laboratory of Flexible Electronics of Zhejiang Province Ningbo Institute of Northwestern Polytechnical UniversityNingbo 315103China The Institute of Flexible Electronics(IFE Future Technologies)Xiamen UniversityXiamen 361005China Wuhan National Laboratory for Optoelectronics-Advanced Biomedical Imaging Facility Huazhong University of Science and TechnologyWuhan 430074China TANK Medicinal Biology Institute of Xi’an Xi’an 710032China

Hepatic ischemia-reperfusion injury(HIRI)is the cause of postoperative hepatic dysfunction and failure,and even death.As an important biological effector molecule,hydrogen sulfide(H_(2)S)of mitochondria as a gasotransmitter that is usually used to protect against acute HIRI injury.However,the exact relationship between HIRI and mitochondrial H_(2)S remains tangled due to the lack of an effective analytical method.Herein,we have fabricated a mitochondria-targeted H_(2)S-activatable fluorogenic probe(Mito-GW)to explore the stability of mitochondrial H_(2)S and track the changes of mitochondrial H_(2)S during the HIRI.By virtue of pyridinium electropositivity and its amphiphilicity,Mito-GW could accumulate in mitochondria.It goes through an analyte-prompted immolation when reacts with H_(2)S,resulting in the releasing of the fluorophore(GW).Therefore,the extent of Mito-GW conversion to GW can be used to evaluate the changes of mitochondrial H_(2)S level in living cells and tissues.As proof-of-principle,we have used MitoGW to demonstrate the mitochondria H_(2)S-levels increase and then decrease during HIRI in vitro and in vivo.Our research highlights the tremendous potential of Mito-GW as a mitochondrial H_(2)S fluorogenic probe in elucidating the pathogenesis of HIRI,providing a powerful tool for promoting future research on hepatology.

关键词： Mitochondria-targeted Bioimaging H2S-activatable Fluorogenic probe Hepatic ischemia-reperfusion injury(HIRI)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Amine-functionalized hierarchically porous carbon supported Pd nanocatalysts for highly efficient H2 generation from formic acid with fast-diffusion channels

引用

Journal of Energy Chemistry 2023年第1期76卷 249-258,I0007页

作者： Xianzhao Shao Xinyi Miao Fengwu Tian miaomiao bai Xiaosha Guo Wei Wang Zuoping Zhao Xiaohui Ji Miyi Li Fangan Deng Shaanxi Key Laboratory of Catalysis School of Chemistry and Environment ScienceShanxi University of TechnologyHanzhong 723001ShaanxiChina School of Mathematics and Computer Science Shanxi University of TechnologyHanzhong 723001ShaanxiChina State Key Laboratory of Qinba Bio-Resource and Ecological Environment Shaanxi University of TechnologyHanzhong 723001ShaanxiChina School of Chemistry and Chemical Engineering Beijing Institute of TechnologyBeijing 102488China

Formic acid(FA)has come to be considered a potential candidate for hydrogen storage,and the development of efficient catalysts for H2releasing is crucial for realizing the sustainable process from FA.Herein,we have developed the ultrafine Pd nanoparticle(NPs)with amine-functionalized carbon as a support,which was found to show an excellent catalytic activity in H_(2)generation from FA dehydrogenation.The synergetic mechanism between amine-group and Pd active site was demonstrated to facilitate H2generation byβ-hydride elimination.Moreover,the texture of support for Pd NPs also plays an important role in determining the reactivity of FA,since the diffusion of gaseous products makes the kinetics of diffusion as a challenge in this high performance Pd catalysts.As a result,the as-prepared Pd/NH_(2)-TPC catalyst with the small sized Pd nanoparticles and the hierarchically porous structures shows a turnover of frequency(TOF)value of 4312 h^(-1)for the additive-free FA dehydrogenation at room temperature,which is comparable to the most promising heterogeneous catalysts.Our results demonstrated that the intrinsic catalytic activities of active site as well as the porous structure of support are both important factors in determining catalytic performances in H2generation from FA dehydrogenation,which is also helpful to develop high-activity catalysts for other advanced gas-liquid-solid reactions systems.

关键词： Hierarchically carbon Diffusion Formic acid Hydrogen production Palladium nanoparticles

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Adaptive T cell immunotherapy in cancer

引用

Science China(Life Sciences) 2021年第3期64卷 363-371页

作者： Dongdong Ti miaomiao bai Xiaolei Li Jianshu Wei Deyun Chen Zhiqiang Wu Yao Wang Weidong Han Bio-therapeutic Department Chinese PLA General HospitalBeijing 100853China

Impaired tumor-specific effector T cells contribute to tumor progression and unfavorable clinical outcomes. As a compensatoryT cell-dependent cancer immunoediting strategy, adoptive T cell therapy (ACT) has achieved encouraging therapeutic results,and this strategy is now on the center stage of cancer treatment and research. ACT involves the ex vivo stimulation and expansionof tumor-infiltrating lymphocytes (TILs) with inherent tumor reactivity or T cells that have been genetically modified to expressthe cognate chimeric antigen receptor or T cell receptor (CAR/TCR), followed by the passive transfer of these cells into alymphodepleted host. Primed T cells must provide highly efficient and long-lasting immune defense against transformed cellsduring ACT. Anin-depth understanding of the basic mechanisms of these living drugs can help us improve upon currentstrategies and design better next-generation T cell-based immunotherapies. From this perspective, we provide an overview ofcurrent developments in different ACT strategies, with a focus on frontier clinical trials that offer a proof of principle. Meanwhile,insights into the determinants of ACT are discussed, which will lead to more rational, potent and widespread applicationsin the future.

关键词： adoptive T cell therapy tumor-infiltrating lymphocytes chimeric antigen receptor T cell receptor

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Imbalanced dietary methionine-to-sulfur amino acid ratio can affect amino acid profiles, antioxidant capacity, and intestinal morphology of piglets

引用

Animal Nutrition 2020年第4期6卷 447-456页

作者： miaomiao bai Lei Wang Hongnan Liu Kang Xu Jinping Deng Ruilin Huang Yulong Yin College of Animal Science South China Agricultural UniversityGuangzhou 510642China Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry ProductionKey Laboratory of Agro-ecological Processes in Subtropical RegionHunan Provincial Engineering Research Center for Healthy Livestock and Poultry ProductionScientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-CentralMinistry of AgricultureInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China Laboratory of Animal Nutrition and Human Health Hunan International Joint Laboratory of Animal Intestinal Ecology and HealthCollege of Life ScienceHunan Normal UniversityChangsha 410081China Hunan Co-Innovation Center of Safety Animal Production(CICSAP) Changsha 410128China

Animal protein sources such as fishmeal and plasma powder are excellent and indispensable sources of energy,amino acid s,and minerals in animal production.Amino acid imbalance,especially methionine-to-sulfur amino acid(Met:SAA)ratio,caused by an imbalance of animal protein meal leads to growth restriction.This study was conducted to evaluate the effects of imbalanced Met:SAA ratio supplementation of different animal protein source diets on growth performance,plasma amino acid profiles,antioxidant capacity and intestinal morphology in a piglet model.Twenty-four weaned piglets(castrated males;BW=10.46±0.34 kg),assigned randomly into 3 groups(8 piglets/group),were fed for 28 d.Three experimental diets of equal energy and crude protein levels were as follows:1)a corn-soybean basal diet with a Met:SAA ratio at 0.51(BD);2)a plasma powder diet with a low Met:SAA ratio at 0.41(L-MR);3)a fishmeal diet with a high Met:SAA ratio at0.61(H-MR).Results revealed that compared to BD,L-MR significantly decreased(P<0.05)the activities of plasma total antioxidant capacity and glutathione peroxidase,plasma amino acid profiles,and significantly reduced(P<0.05)villus height and crypt depth in the duodenum and jejunum.Additionally,L-MR significantly reduced(P<0.05)the mRNA expression level of solute carrier family 7 member 9(SIC7 A9)in the ileum,and significantly increased(P<0.05)mRNA expression levels of zonula occludens-1(ZO-1)in the duodenum,and Claudin-1,ZO-1,sodium-coupled neutral amino acid transporters 2(SNAT2)and SIC7 A7 in the j ejunum.HMR significantly increased(P<0.05)plasma SAA levels,and significantly reduced(P<0.05)average daily feed intake,villus height,and villus height-to-crypt depth(VH:CD)ratio in the ileum compared to BD.In conclusion,L-MR may result in oxidative stress and villous atrophy but proves beneficial in improving intestinal barrier function and the activity of amino acid transporters for compensatory growth.H-MR may impair intestinal growth and development for weaned piglets.The research provides a guidanc

关键词： Animal protein source Methionine-to-sulfur amino acid ratio Antioxidant capacity Intestinal morphology Piglet

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

两种冷季型草坪草的EMS诱变及突变体筛选

引用

草业科学 2024年第4期41卷 810-821页

作者：卢苗苗谢明芳白邦琴眭顺照马婧西南大学园艺园林学院重庆400715 重庆市花卉工程技术研究中心重庆400715 南方山地园艺学教育部重点实验室重庆400715

为确定甲基磺酸乙酯(EMS)诱变多年生黑麦草‘爱神特’(Lolium perenne‘Accent’)和高羊茅‘赤道’(Festuca arundinacea‘Equator’)种子的最佳条件,获得性状稳定的突变材料和后代。利用不同浓度EMS在不同时间下诱变两个草坪草种,根据... 详细信息

为确定甲基磺酸乙酯(EMS)诱变多年生黑麦草‘爱神特’(Lolium perenne‘Accent’)和高羊茅‘赤道’(Festuca arundinacea‘Equator’)种子的最佳条件,获得性状稳定的突变材料和后代。利用不同浓度EMS在不同时间下诱变两个草坪草种,根据半致死浓度确定诱变组合。通过形态学、SCoT分子标记和生理指标测定筛选M1和M2代突变苗。结果表明:使用0.8%EMS溶液处理两个草坪草种36 h后播种,发芽率接近50%,诱变效果最佳。以该参数大规模诱变后,M1代两个草坪草种诱变株移栽成活933株,再经短期干旱胁迫,共筛选出18株耐旱单株。对M2代诱变株形态学分析发现,有叶面狭窄型和根长增长型两种突变类型。利用SCoT分子标记技术验证M2代诱变株发现,筛选的5条SCoT标记引物对20份草坪草样品扩增后,多态性比率为84.09%。其中SCoT-35引物扩增产物条带最为清晰稳定,诱变株与野生型差异明显。M2代三叶期变异单株经干旱、高温胁迫处理后,丙二醛含量、脯氨酸含量及相对电导率呈升高趋势,叶绿素含量呈下降趋势。最终得到两株多年生黑麦草耐旱突变体A5和A8,1株高羊茅耐旱突变体B9;两株多年生黑麦草耐热突变体A8和B9,1株高羊茅耐旱耐热突变体B9。本研究结果为草坪草抗性品种选育提供了帮助。

关键词：草坪草化学诱变种子萌发形态学分析 SCoT分子标记技术突变体耐旱性耐热性

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Reduced dietary nitrogen with a high Lys:CP ratio restricted dietary N excretion without negatively affecting weaned piglets

引用

Animal Nutrition 2019年第2期5卷 115-123页

作者： Hongnan Liu Li Wu Hui Han Yuying Li Lijian Wang Jie Yin Wenjun Fan miaomiao bai Jiming Yao Xingguo Huang Tiejun Li Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process Key Laboratory of Agro-Ecological Processes in Subtropical RegionNational Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry ProductionInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha410125China Hunan Co-Innovation Center of Animal Production Safety Changsha410128China University of Chinese Academy of Sciences Beijing100039China Department of Animal Science Hunan Agriculture UniversityChangsha410128China Guangdong Wangda Group Academician Workstation for Clean Feed Technology Research and Development in Swine Guangdong Wangda Group Co.Ltd.Guangzhou510663China

We hypothesized that balancing the content of exogenous amino acids, especially lysine, to reduce protein content in swine diets could reduce nitrogen(N) pollution associated with animal husbandry. Two experiments(45 d each experiment) were performed on weaned piglets(Duroc x Landrace x Yorkshire,28 d of age) to test this and to determine the optimal lysine to crude protein(Lys:CP) ratio in diet. In Exp. 1,12 piglets(6 replicates [n = 6]) were fed diets containing different levels of CP(17% and 20%) but the same level of Lys, Increased CP content resulted in significant increases(P < 0.05) of average daily gain(ADG), average daily feed intake(ADFI), and body weight(BW);but did not affect the feed to gain ratio. In Exp. 2,24 piglets(8 replicates [n = 8]) were fed 1 of 3 diets as follows: 1) 20% CP with a regular Lys:CP ratio(6.23%, control);2) 17% CP with a reduced Lys:CP ratio(6.14%, LL);or 3) 17% CP with a standard Lys:CP ratio(7.32%, SL). The ADG, final BW, serum concentrations of growth hormone and insulin-like growth factor-1, villus height in the jejunum, and villus height to crypt depth ratio were the lowest in piglets fed LL diet, whereas blood urea N concentration was the lowest and the value of lipase activity was the highest in the piglets fed SL diet. The SL diet did not affect growth performance,intestinal morphology, or serum hormone concentrations, indicating that reduced dietary N with a high Lys:CP ratio can efficiently reduce dietary N excretion without negatively affecting weaned piglets.

关键词： Protein Lysine Feeding Digestion Nutrition Genetic mapping

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Compensation effects of coated cysteamine on meat quality, amino acid composition, fatty acid composition, mineral content in dorsal muscle and serum biochemical indices in finishing pigs offered reduced trace minerals diet

引用

Science China(Life Sciences) 2019年第11期62卷 1550-1553页

作者： miaomiao bai Hongnan Liu Kang Xu Xiaofeng Zhang baichuan Deng Chengquan Tan Jinping Deng Pingping Bing Yulong Yin National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic ProcessHunan Provincial Engineering Research Center of Healthy LivestockKey Laboratory of Agro-ecological Processes in Subtropical RegionInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China College of Animal Science South China Agricultural UniversityGuangzhou 510642China Hunan Co-Innovation Center of Animal Production Safety Changsha 410128China Hangzhou King Techina Technology Company Academician Expert Workstation Hangzhou King Techina Technology Co.Ltd.Hangzhou 311107China Academics Working Station Changsha Medical UniversityChangsha 410219China

Dear Editor.Trace mineral elements(ME)and amino acids have been demonstrated to be essential bioelements in animal nutrition.If there is a deficiency in ME,a number of biological functions in animals may be affected,including physical growth,psychomotor development,and immunity.Supplementation of pig diets with ME is known to improve the animals'growth,reproduction,and their immunity against oxidative stress and cell damage.

关键词： indices oxidative finishing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Symmetric Mach reflection configuration with asymmetric unsteady solution

引用

Chinese Journal of Aeronautics 2023年第4期36卷 75-78页

作者： Chenyuan bai miaomiao WANG Ziniu WU Ministry of Education Key Laboratory of Fluid Mechanics Beihang UniversityBeijing 100083China Department of Engineering Mechanics Tsinghua UniversityBeijing 100084China

Symmetric Mach reflection in steady supersonic flow has been usually studied by solving a half-plane problem with the symmetric line treated as reflecting surface,thus losing the opportunity to discover antisymmetric flow structures.Here in this paper we treat this problem as an entire-plane problem.Using an unsteady numerical approach,we find that the two sliplines exhibit antisymmetric unsteadiness if the Mach stem height is small while the flow remains symmetric if the Mach stem height is large.The mechanism by which disturbance,generated in the downstream of the flow duct between the two sliplines,propagates upstream is identified and it is also shown that the interaction between the transmitted expansion waves and the sliplines increases the amplitude of the unstable modes.The present study suggests a new type of compressible jet that deserves further studies.

关键词： Antisymmetric solution Mach reflection Shock reflection Supersonic flow

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

2022年中国内地心理援助热线设置情况分析

引用

中国公共卫生 2024年第1期40卷 57-60页

作者：吴霞民赵苗苗马宁白羽陈润滋陆林北京大学第六医院北京大学精神卫生研究所国家卫生健康委员会精神卫生学重点实验室(北京大学)国家精神心理疾病临床医学研究中心(北京大学第六医院)北京100191 上海交通大学医学院附属精神卫生中心上海交通大学中国医院发展研究院心理健康管理研究所上海200030

目的了解中国内地心理援助热线设置和服务情况,为政府相关部门进一步完善心理援助热线建设提供依据。方法由国家精神卫生项目办公室编制心理援助热线设置情况调查表,收集2022年中国内地31个省(自治区、直辖市)精神卫生项目办每月上报公... 详细信息

目的了解中国内地心理援助热线设置和服务情况,为政府相关部门进一步完善心理援助热线建设提供依据。方法由国家精神卫生项目办公室编制心理援助热线设置情况调查表,收集2022年中国内地31个省(自治区、直辖市)精神卫生项目办每月上报公立精神卫生医疗机构和精神卫生防治技术管理机构设立的热线服务号码、服务时间、坐席数、热线咨询员数、接听量,采用描述性统计、Kruskal Wallis检验对热线设置和服务情况进行分析。结果2022年中国内地31省(自治区、直辖市)以公立精神卫生医疗机构和精防机构为主的心理援助热线650条,7 d×24 h全时段的服务热线占68.3%;热线坐席1288个,平均0.9个/100万人口;热线咨询员8152人,平均5.8人/100万人口。2022年每条热线接听量平均为1668次,东部地区(2656次)热线接听量高于中部(1765次)和西部地区(800次)(H=6.159,P<0.05),省级(10665次)热线接听量高于市级(1198次)和区县级(426次)(H=30.417,P<0.01)。结论心理援助热线是公众寻求心理健康服务的适宜形式之一,得到了较高利用。下一步应整合资源,优化热线号码,不断加强宣传,强化专业队伍建设,加强热线服务的规范性,更好地满足公众的需要。

关键词：心理援助热线热线坐席接听量

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

低剂量右美托咪定对老年髋部骨折术后炎症反应及谵妄的影响

引用

首都医科大学学报 2024年第2期45卷 220-225页

作者：崔真于淼淼孔月月柯丽张柳白颖王郝首都医科大学附属北京积水潭医院重症医学科北京100035

目的评估预防性使用低剂量右美托咪定(dexmedetomidine,DEX)对老年髋部骨折患者术后炎症反应的影响。方法将入住重症监护病房(intensive care unit,ICU)的老年髋部骨折术后患者采用数字表法随机分配到DEX组或对照组,即刻分别给予DEX 0.1... 详细信息

目的评估预防性使用低剂量右美托咪定(dexmedetomidine,DEX)对老年髋部骨折患者术后炎症反应的影响。方法将入住重症监护病房(intensive care unit,ICU)的老年髋部骨折术后患者采用数字表法随机分配到DEX组或对照组,即刻分别给予DEX 0.1μg·kg^(-1)·h^(-1)及0.9%(质量分数)氯化钠注射液,相同液速(0.025 mL·kg^(-1)·h^(-1))静脉泵入。测定给药后0(即刻)、6、12、18 h患者血清降钙素原和白细胞介素-6浓度,记录血流动力学参数(心指数、每搏量指数、平均动脉压、心率)及谵妄的发生。结果两组间降钙素原及白细胞介素-6在各个时间点的差异无统计学意义(P>0.05),DEX组出现谵妄的例数显著低于对照组(P=0.034)。DEX组额外使用阿片类镇痛药物的例数(P=0.039)以及出现高血压的例数(P=0.012)显著低于对照组。结论低剂量DEX未显著降低老年髋部骨折患者术后的炎症指标,但有助于减少老年髋部骨折患者谵妄的发生及阿片类药物的使用。低剂量DEX对于血流力学影响较小,对术后高血压的患者可能具有一定循环保护作用。

关键词：右美托咪定老年髋部骨折谵妄血流动力学炎症反应

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：