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Geniposide protects human neuroblastoma SH-SY5Y cells against corticosterone-induced injury

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Neural Regeneration Research 2011年第21期6卷 1618-1622页

作者： Liping Chen Fawei Wang miao geng Hongyan Chen Dongmei Duan Department of Traditional Chinese Medicine General Hospital of PLA Beijing 100853 China Institute of Gerontology General Hospital of PLA Beijing 100853 China

In vitro cultured human neuroblastoma SH-SY5Y cells were pretreated with 50 or 5 ug/mL geniposide for 12 hours and exposed to 400 umol/L corticosterone. Corticosterone exposure in cultures not pretreated with geniposide resulted in inhibited cell growth, reduced cell survival, and increased P53 and P21 protein expression. However, in geniposide pretreated SH-SY5Y cells, cell viability and the number of cells in the G2 phase of the cell cycle were significantly increased, P21 and P53 protein expression was reduced, and cell apoptosis was inhibited following corticosterone exposure. These results indicate that geniposide can protect SH-SY5Y cells against high-dose corticosterone-induced injury.

关键词： Geniposide SH-SY5Y cells corticosterone protein expression apoptosis

Protective effects of ginsenoside Rg1 against hydrogen peroxide-induced injury in human neuroblastoma cells

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Neural Regeneration Research 2016年第7期11卷 1159-1164页

作者： Zhi-gao Sun Li-ping Chen Fa-wei Wang Cheng-yong Xu miao geng Department of Traditional Chinese Medicine Hainan Branch Hospital of Chinese PLA General Hospital Department of Traditional Chinese Medicine Chinese PLA General Hospital Institute of Gerontology Chinese PLA General Hospital

The active ingredient of ginseng,ginsenosides Rg1,has been shown to scavenge free radicals and improve antioxidant *** study hypothesized that ginsenosides Rg1 has a protective role in human neuroblastoma cells injured by *** Rg1 at different concentrations（50 and 100 μM） was used to treat H2O2（150 μM）-injured SH-SY5 Y *** demonstrated that ginsenoside Rg1 elevated the survival rate of SH-SY5 Y cells injured by H2O2,diminished the amount of leaked lactate dehydrogenase,and increased superoxide dismutase *** Rg1 effectively suppressed caspase-3 immunoreactivity,and contributed to heat shock protein 70 gene expression,in a dose-dependent *** results indicate that ginsenoside Rg1 has protective effects on SH-SY5 Y cells injured by H2O2 and that its mechanism of action is associated with anti-oxidation and the inhibition of apoptosis.

关键词： ginsenoside neuroblastoma peroxide injured protective caspase dehydrogenase suppressed manner antioxidant

Shuyusan-containing serum protects SH-SY5Y cells against corticosterone-induced impairment

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Neural Regeneration Research 2013年第22期8卷 2060-2068页

作者： Liping Chen Zhigao Sun Fawei Wang Chengyong Xu miao geng Hongyan Chen Dongmei Duan Department of Traditional Chinese Medicine Hainan Branch of Chinese PLA General Hospital Department of Internal Medicine Hainan Branch of Chinese PLA General Hospital Institute of Geriatrics General Hospital of Chinese PLA

The Chinese herb Shuyusan, whose main constituent is jasminoidin, has been shown to protect SH-SY5Y cells against corticosterone-induced damage. SH-SY5Y cells injured by 400 μmol/L cor- ticosterone were treated with 5 and 30 μg/mL Shuyusan-containing serum. Results revealed that Shuyusan-containing serum elevated the survival rate of SH-SY5Y cells, reduced Bax expression, increased Bcl-2 expression, markedly elevated brain-derived neurotrophic factor mRNA expression, and blocked cell apoptosis. Moreover, the effect of high-dose （30 μg/mL） Shuyusan-containing se- rum was more remarkable. Therefore, Shuyusan-containing serum appears to protect SH-SY5Y cells against corticosterone-induced impairment by adjusting the expression of apoptosis-associ- ated proteins and brain-derived neurotrophic factor. Moreover, high-dose Shuyusan-containing se- rum has a protective effect on high-dose corticosterone-induced impairment.

关键词： neural regeneration traditional Chinese medicine Shuyusan-containing serum SH-SY5Y cells corticosterone Bcl-2 Bax apoptosis brain-derived neurotrophic factor grants-supported paper neuroregeneration

Screening of HLA-A2 restricted CTL epitopes using molecular simulation

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Chinese Science Bulletin 2003年第18期48卷 1962-1966页

作者： geng miao WU Yuzhang LIN Zhihua JIA Zhengcai ZHOU Wei ZOU Liyun Institute of Immunology PLA Third Military Medical UniversityChongqing 400038China College of Bioengineering Chongqing Institute of TechnologyChongqing 400050China

A new technology has been established to screen CTL epitope by using molecular simulation and mo-lecular dynamics. By using this technology, four peptides of MAGE-2 were screened and further identified by peptide binding assay and cytotoxic assay. Results showed that three candidate peptides had higher affinity to HLA-A2 molecule and were able to pulse CTL activities in vitro in human PBMC. The results indicated that identification of CTL epi-topes with computer assistant could overcome the compli-cated experimental steps, particularly the peptide elution step, and could directly observe peptide-MHC-Ⅰ compound binding pattern and could be used to screen CTL epitopes quickly.

关键词： CTL epitope HLA-A2 identification computer assis-tant molecular simulation.

Protective effects of baicalin on amyloid beta 25-35-induced apoptosis in human neuroblastoma SH-SY5Y cells

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Neural Regeneration Research 2010年第22期5卷 1739-1744页

作者： miao geng Hongyan Chen Jianhua Wang Yazhuo Hu Jianwei Liu Jing Liu Jingkun Pan Yuhong Gao Institute of Geriatrics Division of South Building Chinese PLA General Hospital Beijing 100853 China

Baicalin, a type of flavanoid, effectively prevents cellular apoptosis induced by various factors. However, little evidence is available regarding its role on amyloid β （Aβ） -induced neuronal apoptosis. The present study investigated the protective mechanisms of baicalin on Aβ-induced neuronal apoptosis. Flow cytometry and cation dye 5, 5＇, 6, 6＇-tetrachloro-1, 1＇, 3, 3＇-tetraethyl- benzimidazoly lcarbocyanine iodide （JC-1） were employed to measure mitochondrial membrane potential, and nitric oxide secretion and apoptotic-related factors, such as caspase-3, were comprehensively analyzed. Results demonstrated a protective effect of baicalin on Aβ-treated SH-SY5Y cell viability; the rate of apoptosis decreased, nitric oxide generation and expression of caspase-3 were effectively inhibited, and mitochondrial membrane potential was effectively protected. Baicalin inhibited Aβ-induced neuronal apoptosis via multiple targets and multiple pathways, such as the inhibition of free radical damage, reduction of caspase-3 expression, and protection of normal mitochondrial functions.

关键词： baicalin neuron apoptosis traditional Chinese medicine caspase-3 amyloid β neural regeneration

An X-band 22.5°/45° digital phase shifter based on switched filter networks

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Journal of Semiconductors 2017年第6期38卷 93-96页

作者： Pengpeng Sun Hui Liu miao geng Rong Zhang Qi Wang Weijun Luo Institute of Microelectronics Chinese Academy of Sciences Beijing 100029 China University of Chinese Academy of Sciences Beijing 100049 China School of Advanced Materials and Nanotechnology Xidian University Xi'an 710049 China

The design approach and performance of a 22.5°/45°digital phase shifter based on a switched filter network for X-band phased arrays are *** the MMIC phase shifters are fabricated employing a 0.25 m gate GaAs pHEMT process and share in the same chip size of 0.82 1.06 mm^*** measurement results of the proposed phase shifters over the whole operating frequency range show that the phase shift error is less than 22.5°˙2.5°,45°˙3.5°,which shows an excellent agreement with the simulated performance,the insertion loss is within the range of 0.9–1.2 dB for the 22.5° phase shifter and 0.9–1.4 dB for the 45°phase shifter,and the input/output return loss is better than –12.5 and –11 dB *** also achieve the similar P（1dB）continuous wave power handing capability of 24.8 dBm at 10 *** phase shifters show a good phase shift error,insertion loss and return loss in the X-band（40%）,which can be employed into the wide bandwidth multi-bit digital phase shifter.

关键词： phase shifter switched filter X-band GaAs pHEMT

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黄河泥沙分选装置设计与试验研究

人民黄河 2014年第5期36卷 24-26页

作者：耿淼蕊郑州大学水利与环境学院河南郑州450001

在分析黄河泥沙分选现状的基础上,介绍了利用磁铁和离心力原理研制的黄河泥沙分选装置的工作原理及其工作参数,并进行了典型铁沙和粗、中、细泥沙的室内分选试验。结果表明:该装置揭示了不同粒径泥沙在分选筒内旋转水体作用下的沉降分... 详细信息

在分析黄河泥沙分选现状的基础上,介绍了利用磁铁和离心力原理研制的黄河泥沙分选装置的工作原理及其工作参数,并进行了典型铁沙和粗、中、细泥沙的室内分选试验。结果表明:该装置揭示了不同粒径泥沙在分选筒内旋转水体作用下的沉降分布规律,其具有结构简单、操作维修方便、结实耐用、节能高效、成本低廉、可连续作业等特点。

关键词：试验研究分选装置泥沙黄河

广东海上丝绸之路博物馆环境空气腐蚀性污染调查报告

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博物院 2020年第4期 119-125页

作者：耿苗广东海上丝绸之路博物馆阳江529536

广东海上丝绸之路博物馆(以下简称海丝馆)地处海岛,常年处于高湿、高温、高盐的大气环境中,这对文物的安全保存极为不利。为了解掌握海丝馆内环境污染腐蚀状态,本研究利用美国Purafil公司研发的腐蚀试片检测技术,在海丝馆库房、展厅、... 详细信息

广东海上丝绸之路博物馆(以下简称海丝馆)地处海岛,常年处于高湿、高温、高盐的大气环境中,这对文物的安全保存极为不利。为了解掌握海丝馆内环境污染腐蚀状态,本研究利用美国Purafil公司研发的腐蚀试片检测技术,在海丝馆库房、展厅、沉船遗址区等进行了为期30天的测试实验;同时运用离子色谱法对馆内空气阴离子含量进行了测定。数据显示,海丝馆内空气处于高浓度的酸性腐蚀性气体环境中,这严重威胁各类材质文物的长期安全保存,应采取有效手段改善文物保护环境,为文物创造稳定的保存条件。

关键词：博物馆微环境广东海上丝绸之路博物馆腐蚀试片检测技术 “南海Ⅰ号” 离子色谱法

舞蹈教学在风格与个性之间的作用——关于中国古典舞基训教学实践的思考

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商丘职业技术学院学报 2008年第6期7卷 101-102页

作者：耿苗安阳师范学院音乐学院河南安阳455000

如何处理好舞蹈艺术中风格与个性之间的关系,始终是舞蹈理论界、教育界关注的问题。在舞蹈艺术和风格之间,舞种的分类体现了各自独特的形象内容;在舞蹈艺术与个性之间,剧目的创作给了肢体语言更为丰富的诠释。风格回炉与个性开发真正在... 详细信息

如何处理好舞蹈艺术中风格与个性之间的关系,始终是舞蹈理论界、教育界关注的问题。在舞蹈艺术和风格之间,舞种的分类体现了各自独特的形象内容;在舞蹈艺术与个性之间,剧目的创作给了肢体语言更为丰富的诠释。风格回炉与个性开发真正在教学过程中具有对立统一的辨证关系,二者既相互制约,此消彼长,同时又相互促进,共同发展。只有通过正确、恰当、适时、有计划、循序渐进的舞蹈教学,才能使风格和个性两方面有机互补的结合在一起,才会体现最终不断裂的风格与个性。

关键词：舞蹈教学风格个性