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Petroleum Science 2017年第1期14卷 126-137页

作者： wan Cheng Yan Jin mian Chen Guo-Sheng Jiang State Key Laboratory of Petroleum Resources and Prospecting China University of Petroleum Faculty of Engineering China University of Geosciences

A multi-casing structure in drilling engineering can be considered as an inhomogeneous body consisting of many different materials. The mechanical behavior of the inhomogeneous body in an infinite domain is very com- plicated. In this paper, a detailed expression about the fictitious stress method of the boundary element method （BEM） is demonstrated for the inhomogeneous body. Then the fictitious stress method is deployed to investigate the stresses for the multi-casing structure under non-uniform loading conditions and an irregular wellbore. Three examples of the multi-casing structure in the borehole imply the high effectiveness of BEM for complex geometries related to the borehole in an infinite formation. The effects of casing eccentricity and the interfacial gap on the stress field are discussed. The eccentric casing takes the potential yield when the eccentric orientation is along the direction of Sh. Under different eccentric orientations, the yon Mises stress in the casing increases with increasing degree of eccentricity. The radial stress in the multi-casing structure is always continuous along the radius, but the circumferential stress is discontinuous at the interface. The radial stress decreases and the circumferential stress increases with the increasing of the interfacial gap between the adjacent materials.

关键词： Displacement discontinuity method Fictitiousstress method Drilling mechanics Wellbore stressconcentration Inhomogeneous body

Metabolic-epigenetic nexus in regulation of stem cell fate

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World Journal of Stem Cells 2022年第7期14卷 490-502页

作者： Yi Liu Di-Xin Cui Yue Pan Si-Han Yu Li-Wei Zheng Mian Wan State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesDepartment of Pediatric DentistryWest China Hospital of StomatologySichuan UniversityChengdu 610041Sichuan ProvinceChina State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesDepartment of Cariology and EndodonticsWest China Hospital of StomatologySichuan UniversityChengdu 610041Sichuan ProvinceChina

Stem cell fate determination is one of the central questions in stem cell biology,and although its regulation has been studied at genomic and proteomic levels,a variety of biological activities in cells occur at the metabolic level.Metabolomics studies have established the metabolome during stem cell differentiation and have revealed the role of metabolites in stem cell fate determination.While metabolism is considered to play a biological regulatory role as an energy source,recent studies have suggested the nexus between metabolism and epigenetics because several metabolites function as cofactors and substrates in epigenetic mechanisms,including histone modification,DNA methylation,and microRNAs.Additionally,the epigenetic modification is sensitive to the dynamic metabolites and consequently leads to changes in transcription.The nexus between metabolism and epigenetics proposes a novel stem cell-based therapeutic strategy through manipulating metabolites.In the present review,we summarize the possible nexus between metabolic and epigenetic regulation in stem cell fate determination,and discuss the potential preventive and therapeutic strategies via targeting metabolites.

关键词： Metabolism Epigenetic regulation Stem cell fate Nexus effect

New insight into dental epithelial stem cells:Identification,regulation,and function in tooth homeostasis and repair

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World Journal of Stem Cells 2020年第11期12卷 1327-1340页

作者： Lu Gan Ying Liu Di-Xin Cui Yue Pan Mian Wan State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Pediatric Dentistry West China Hospital of StomatologySichuan UniversityChengdu 610041Sichuan ProvinceChina State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Cariology and Endodontics West China Hospital of StomatologySichuan UniversityChengdu 610041Sichuan ProvinceChina

Tooth enamel,a highly mineralized tissue covering the outermost area of teeth,is always damaged by dental caries or trauma.Tooth enamel rarely repairs or renews itself,due to the loss of ameloblasts and dental epithelial stem cells(DESCs)once the tooth erupts.Unlike human teeth,mouse incisors grow continuously due to the presence of DESCs that generate enamel-producing ameloblasts and other supporting dental epithelial lineages.The ready accessibility of mouse DESCs and wide availability of related transgenic mouse lines make mouse incisors an excellent model to examine the identity and heterogeneity of dental epithelial stem/progenitor cells;explore the regulatory mechanisms underlying enamel formation;and help answer the open question regarding the therapeutic development of enamel engineering.In the present review,we update the current understanding about the identification of DESCs in mouse incisors and summarize the regulatory mechanisms of enamel formation driven by DESCs.The roles of DESCs during homeostasis and repair are also discussed,which should improve our knowledge regarding enamel tissue engineering.

关键词： Dental epithelial stem cells Tissue engineering Label-retaining cells Lineage tracing Single-cell sequencing

Antibacterial ability and biocompatibility of fluorinated titanium by plasma-based surface modification

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Rare Metals 2022年第2期41卷 689-699页

作者： mian Chen Xiao-Qiao wang Er-Lin Zhang Yi-Zao wan Jian Hu Jiangxi Key Laboratory of Nanobiomaterials Institute of Advanced MaterialsEast China Jiaotong UniversityNanchang 330013China Key Laboratory for Anisotropy and Texture of Materials(ATM) School of Material Science and EngineeringNortheastern UniversityShenyang 110819China

Biomaterial surfaces with satisfied antibacterial activity and appropriate cytocompatibility are a pressing clinical need for orthopedic and dental implants.Fluorinecontaining biomaterials have been demonstrated to obtain antibacterial activity and osteogenic property,while the effect of fluorine chemical compositions on antibacterial property and cytocompatibility is rarely studied.To this end,the coatings with different fluorine chemical compositions on titanium surface were prepared by plasma treatment to verify the antibacterial ability and cytocompatibility of fluorinated surfaces.Their antibacterial ability was evaluated by using Staphylococcus aureus,and the cell compatibility was investigated with MC3T3-E1 cells in vitro.The results show that both fluorocarbon coating and metal fluorides coating exhibited a hydrophilic and nano-scaled roughness.Rather than the fluorocarbon coating,the coating composed of metal fluorides presented satisfied bactericide effect and excellent cytocompatibility.The antibacterial mechanism is associated with the metal fluorides and released fluoride ion.This work would provide novel sight in optimizing the surface modification method of fluorinated biomaterials for biomedical applications.

关键词： Titanium Plasma-based technology Fluorine Antibacterial property Cytocompatibility

行为转变理论的教育干预对酒依赖患者健康信念和应对方式的影响

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中国健康心理学杂志 2023年第11期31卷 1612-1617页

作者：万勉许世阳湖北省宜昌市优抚医院精神科 443000 宜昌市中心人民医院精神科

目的:探讨基于行为转变理论的教育干预对酒依赖患者健康信念和应对方式的影响。方法:将2021年4月-2022年4月于某院住院治疗的80例酒依赖患者依据入院时间分为对照组和研究组,两组各40例。对照组予以常规健康教育干预,研究组予以基于行... 详细信息

目的:探讨基于行为转变理论的教育干预对酒依赖患者健康信念和应对方式的影响。方法:将2021年4月-2022年4月于某院住院治疗的80例酒依赖患者依据入院时间分为对照组和研究组,两组各40例。对照组予以常规健康教育干预,研究组予以基于行为转变理论的教育干预。比较两组干预前后健康信念、应对方式,比较两组干预后3个月酒精依赖度及干预前后生活质量,并评估其干预满意度。结果:干预后,两组健康信念量表各维度评分均高于干预前,且研究组各评分显著高于对照组(t=3.685,6.822,8.653,6.223,4.528;P<0.05);干预后,两组“面对”应对方式评分均升高,“回避”、“屈服”应对方式评分均降低,且研究组“面对”应对方式评分显著高于对照组,“回避”、“屈服”应对方式评分显著低于对照组(t=3.690,-3.930,-5.454;P<0.05);干预后3个月,研究组的酒精依赖度明显轻于对照组(Z=5.681,P<0.05);干预后,两组生活质量综合评定问卷(GQOLI)各维度评分均升高,且研究组各评分显著高于对照组(t=2.718,4.177,5.178,2.645;P<0.05);研究组的干预满意度显著高于对照组(χ^(2)=6.646,P<0.05)。结论:对酒精依赖患者应用基于行为改变理论的教育干预能有效提升其健康信念,改善其应对方式,减轻其对酒精的依赖程度,提高患者生活质量,其且该干预方法临床满意度高。

关键词：行为转变理论健康信念应对方式酒精依赖生活质量

Understanding hydraulic fracture propagation behavior in tight sandstone–coal interbedded formations: an experimentalinvestigation

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Petroleum Science 2019年第1期16卷 148-160页

作者： Peng Tan Yan Jin Liang Yuan Zhen-Yu Xiong Bing Hou mian Chen Li-Ming wan State Key Laboratory of Petroleum Resources and Engineering China University of Petroleum CNPC Engineering Technology R&D Company Limited SINOPEC Shanghai O shore Oil & Gas Company

Whether hydraulic fractures could connect multiple gas zones in the vertical plane is the key to fracturing treatment to jointly exploit coalbed methane and tight sandstone gas through integrative hydraulic fracturing in tight sandstone–coal interbedded formations. Laboratory true triaxial hydraulic fracturing experiments were conducted on layered specimens with di erent combination types of natural sandstone and coal to simulate the propagation behavior of hydraulic fractures. The effects of the fracture initiation position, fracturing fluid viscosity and injection rate were discussed. The results showed that di erent fracture morphologies could be found. When initiating from coal seams, three patterns of fracture initiation and propagation were obtained:(1) The main hydraulic fracture initiated and propagated along the natural fractures and then diverged due to the effects of in situ stress and formed secondary fractures.(2) The hydraulic fracture initiated and propagated in the direction of the maximum horizontal stress.(3) Multiple fractures initiated and propagated at the same time. With the same fracturing fluid viscosity and injection rate, the hydraulic fractures initiating in sandstones had greater chances than those in coal seams to penetrate interfaces and enter neighboring layers. Excessively small or large fracturing fluid viscosity and injection rate would do harm to the vertical extension height of the induced fracture and improvement of the stimulated reservoir volume. Compared with operation parameters(fracturing fluid viscosity and injection rate), the natural weak planes in coals were considered to be the key factor that a ected the fracture propagation path. The experimental results would make some contributions to the development of tight sandstone–coal interbedded reservoirs.

关键词： Hydraulic fracturing Fracture propagation Sandstone-coal interbed Layered formation

Epigenetic regulation of dental pulp stem cells and its potential in regenerative endodontics

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World Journal of Stem Cells 2021年第11期13卷 1647-1666页

作者： Ying Liu Lu Gan Di-Xin Cui Si-Han Yu Yue Pan Li-Wei Zheng Mian Wan State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesDepartment of Pediatric DentistryWest China Hospital of StomatologySichuan UniversityChengdu 610041Sichuan ProvinceChina State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesDepartment of Cariology and EndodonticsWest China Hospital of StomatologySichuan UniversityChengdu 610041Sichuan ProvinceChina

Regenerative endodontics(RE)therapy means physiologically replacing damaged pulp tissue and regaining functional dentin–pulp complex.Current clinical RE procedures recruit endogenous stem cells from the apical papilla,periodontal tissue,bone marrow and peripheral blood,with or without application of scaffolds and growth factors in the root canal space,resulting in cementum-like and bone-like tissue formation.Without the involvement of dental pulp stem cells(DPSCs),it is unlikely that functional pulp regeneration can be achieved,even though acceptable repair can be acquired.DPSCs,due to their specific odontogenic potential,high proliferation,neurovascular property,and easy accessibility,are considered as the most eligible cell source for dentin–pulp regeneration.The regenerative potential of DPSCs has been demonstrated by recent clinical progress.DPSC transplantation following pulpectomy has successfully reconstructed neurovascularized pulp that simulates the physiological structure of natural pulp.The self-renewal,proliferation,and odontogenic differentiation of DPSCs are under the control of a cascade of transcription factors.Over recent decades,epigenetic modulations implicating histone modifications,DNA methylation,and noncoding(nc)RNAs have manifested as a new layer of gene regulation.These modulations exhibit a profound effect on the cellular activities of DPSCs.In this review,we offer an overview about epigenetic regulation of the fate of DPSCs;in particular,on the proliferation,odontogenic differentiation,angiogenesis,and neurogenesis.We emphasize recent discoveries of epigenetic molecules that can alter DPSC status and promote pulp regeneration through manipulation over epigenetic profiles.

关键词： Dental pulp stem cells Regenerative endodontics Epigenetic regulation Noncoding RNAs Histone deacetylase inhibitor DNA methyltransferase inhibitor

针刺联合运动灸鼓膜按摩法治疗神经性耳鸣33例

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中国针灸 2022年第9期42卷 995-996页

作者：王迎冬万红棉山东中医药大学针灸推拿学院济南250013 济南市中医医院针灸科山东济南250012

笔者采用针刺联合运动灸鼓膜按摩法治疗神经性耳鸣33例,现报道如下。1临床资料33例均为2021年2月至2022年2月就诊于济南市中医医院针灸科的神经性耳鸣患者,其中男17例,女16例;年龄最小18岁,最大67岁,平均(41±13)岁;病程最短2个月,最长3... 详细信息

笔者采用针刺联合运动灸鼓膜按摩法治疗神经性耳鸣33例,现报道如下。1临床资料33例均为2021年2月至2022年2月就诊于济南市中医医院针灸科的神经性耳鸣患者,其中男17例,女16例;年龄最小18岁,最大67岁,平均(41±13)岁;病程最短2个月,最长37个月,平均(21.4±8.5)个月。

关键词：神经性耳鸣针刺运动灸鼓膜按摩耳周四穴

蒌叶提取物增强5-氟尿嘧啶对结肠癌细胞HT29和HCT116的生长抑制作用(英文)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2014年第8期15卷 692-700页

作者： Pek Leng NG Nor Fadilah RAJAB Sue mian THEN Yasmin Anum MOHD YUSOF wan Zurinah wan NGAH Kar Yong PIN Mee Lee LOOI Department of Biomedical Sciences Faculty of Allied Health Universiti Kebangsaan Malaysia (UKM) Jalan Raja Muda Abdul Aziz50300 Kuala Lumpur Malaysia UKM Medical Molecular Biology Institute (UMBI) Universiti Kebangsaan Malaysia (UKM) Jalan Yaacob Latiff Bandar Tun Razak Cheras56000 Kuala Lumpur Malaysia School of Biomedical Science University of Nottingham Malaysia Campus43500 Semenyih Selangor Malaysia Department of Biochemistry Faculty of Medicine Universiti Kebangsaan Malaysia (UKM) Jalan Raja Muda Abdul Aziz50300 Kuala Lumpur Malaysia Forest Research Institute Malaysia (FRIM) 52109 Kuala Lumpur Kepong Malaysia School of Biosciences Taylor’s University Lakeside Campus47500 Subang Jaya Selangor Malaysia

研究目的:探讨蒌叶(PB)提取物对5-氟尿嘧啶(5-FU)抑制结肠癌细胞HT29和HCT116生长的影响。研究方法:HT29和HCT116细胞分别给予PB、5-FU以及两种药物联合治疗24小时,应用等效线图法分析PB和5-FU的药效学相互作用,Annexin V/PI染色法检测H... 详细信息

研究目的:探讨蒌叶(PB)提取物对5-氟尿嘧啶(5-FU)抑制结肠癌细胞HT29和HCT116生长的影响。研究方法:HT29和HCT116细胞分别给予PB、5-FU以及两种药物联合治疗24小时,应用等效线图法分析PB和5-FU的药效学相互作用,Annexin V/PI染色法检测HT29和HCT116细胞的凋亡情况,高效液相色谱法排除PB和5-FU间任何可能的相互化学作用。重要结论:联合PB,低剂量5-FU可以在短时间内起到细胞毒作用,而单独应用PB或5-FU治疗较联合治疗可以诱导更多细胞发生凋亡。进一步采用等效线图法分析显示PB和5-FU的联合作用在抑制结肠癌细胞HT29和HCT116的生长中分别体现出协同和拮抗作用。因此可以认为在HT29细胞中,PB使得较低剂量5-FU发挥最大抑制结肠癌细胞生长效果,然而在HCT116细胞中,PB没有显著降低5-FU的药物浓度,说明PB和5-FU的相互作用不仅仅体现在诱导细胞凋亡方面。

关键词：胡椒科蒌叶 5-氟尿嘧啶等效线图法草药-药物相互作用

Spatial signalling mediated by the transforming growth factor-β signalling pathway during tooth formation

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International Journal of Oral Science 2016年第4期8卷 199-204页

作者： Xin-Yu He Ke Sun Ruo-Shi Xu Jia-Li Tan Cai-Xia Pi Mian Wan Yi-Ran Peng Ling Ye Li-Wei Zheng Xue-Dong Zhou State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University West China School of Stomatology Sichuan University Guanghua School of Stomatology Hospital of Stomatology Department of Endodontics Guangdong Provincial Key Laboratory of Stomatology Sun Yat-sen University

Tooth development relies on sequential and reciprocal interactions between the epithelial and mesenchymal tissues, and it is continuously regulated by a variety of conserved and specific temporal-spatial signalling pathways. It is well known that suspensions of tooth germ cells can form tooth-like structures after losing the positional information provided by the epithelial and mesenchymal tissues. However, the particular stage in which the tooth germ cells start to form tooth-like structures after losing their positional information remains unclear. In this study, we investigated the reassociation of tooth germ cells suspension from different morphological stages during tooth development and the phosphorylation of Smad2/3 in this process. Four tooth morphological stages were designed in this study. The results showed that tooth germ cells formed odontogenic tissue at embryonic day （E） 14.5, which is referred to as the cap stage, and they formed tooth-like structures at E16.5, which is referred to as the early bell stage, and E18.5, which is referred to as the late bell stage. Moreover, the transforming growth factor-β signalling pathway might play a role in this process.

关键词： positional information transforming growth factor-13 signalling pathway tooth development