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Development and characterization of novel polymorphic microsatellite markers for the Korean freshwater snail Semisulcospira coreana and cross-species amplification using next-generation sequencing

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Journal of Oceanology and Limnology 2020年第2期38卷 503-508页

作者： Yeon jung PARK mi Nan LEE Eun-mi KIM jung Youn PARK Jae Koo NOH Tae-Jin CHOI jung-Ha KANG Biotechnology Research Division National Institute of Fisheries ScienceBusan 46083Republic of Korea Department of microbiology Pukyoung National UniversityBusan 48513Republic of Korea

Korean freshwater snails of the genus Semisulcospira are widely distributed across East *** has been a very popular nutritional food in Korea,and is an ecologically important water quality indicator because it lives only in clean ***,no microsatellite markers have been generated to study the population genetic diversity of this *** the present study,we developed and characterized 18 novel microsatellite loci from Semisulcospira coreana genomic *** microsatellites were isolated using 454 GS-FLX titanium sequencing and 18 markers were used for genotyping in *** addition,we also tested the cross-species transferability of the microsatellite markers in four additional Semisulcospira *** identified 18 polymorphic loci and the number of alleles per loci,and their polymorphism information content values ranged from 2 to 17 and 0.203 to 0.902,*** observed and expected heterozygosities of the loci ranged from 0.063 to 0.924 and 0.226 to 0.924,*** to the analysis of the cross-species transferability of these markers,four species,***,***,***,and ***,showed a very high transferability(80%–85%).These results show that this set of nuclear markers could be useful for population genetics studies of this species and closely related species.

关键词： Semisulcospira next-generation sequencing(NGS) microsatellite markers cross-species transferability

Inter-observer Reliability of Indicators and Decision of Pattern Identification Using Diagnostic Flowchart with Traditional Korean Medicine

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Chinese Journal of Integrative Medicine 2017年第5期23卷 338-344页

作者： Ju Ah Lee Jeeyoun jung mi mi Ko Myeong Soo Lee Korean Medicine Fundamental Research Division Korea Institute of Oriental Medicine Daejeon 305-811 Republic of Korea Clinical Research Division Korea Institute of Oriental Medicine Daejeon 305-811 Republic of Korea

Objective： To evaluate the consistency of patterns and pattern identification（PI）, and the improvement in the diagnosis of blood stasis after the standard operating procedures（SOPs） were enhanced by using a diagnostic flowchart. Methods： A total of 358 subjects who were admitted by the Jaseng Hospital of Korean Medicine between June 2013 and December 2014 were included in the study. Each patient was independently diagnosed by two experts from the same department. In 2014, the SOPs included the use of a diagnostic flowchart for each indicator. Inter-observer consistency was assessed by simple percent agreement and by the kappa and AC1 statistics. Results： The improvements in inter-observer agreement for the indicators（for al patients） and PI were greater in 2014 than in 2013. In 2013, the j-value measure of agreement between the two experts ranged from ＂poor＂（κ=–0.051） to ＂good＂（κ=0.968）. The AC1 measure of agreement between the experts was generally high for the indicators and ranged from –0.010 to 0.978. In most cases, agreement was considerably lower when it was assessed by the j-values compared with the AC1 values. In 2014, the j-value measure of agreement on the indicators（for the subjects） general y ranged from poor to good, with the values ranging from –0.007 to 0.994. Moreover, the AC1 measure of agreement between the experts was general y high for al of the indicators and ranged from ＂moderate＂（AC1=0.408） to ＂excel ent＂（AC1=0.996）. Conclusions： In four examinations, there was moderate agreement between the clinicians on the PI indicators of blood stasis. To improve clinician consistency（e.g., in the diagnostic criteria used）, it is necessary to analyse the reasons for inconsistency and to improve clinician training.（Trial registration No. KCT0000916）

关键词： blood stasis inter-observer reliability pattern identification traditional Chinese medicine traditional Korean medicine

Recognition of Association Between Blood Stasis Syndrome and Traumatic Injury among Doctors of Korean Medicine:A Cross-Sectional Observation Study

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Chinese Journal of Integrative Medicine 2018年第4期24卷 254-259页

作者： Jeeyoun jung mi mi Ko Ju Ah Lee Myeong Soo Lee Korean Medicine Fundamental Research Division Korea Institute of Oriental Medicine Clinical Research Division Korea Institute of Oriental Medicine

Objective： To investigate the diagnostic indicators and herbal treatments for blood stasis syndrome（BSS） patients with traumatic injuries and to identify the association between BSS and traumatic injury in Korea. Methods： Two-hundred and four patients with traumatic injury were recruited from the Gangnam and Daejeon branches of Jaseng Hospital of Oriental Medicine between June 2014 and December 2014. Two independent doctors of Korean medicine（DKMs） determined the diagnosis of BSS or non-BSS based on the subjects＇ signs and symptoms. The scores assigned to BSS symptoms and DKMs＇ reasons for diagnosing BSS in patients with traumatic injury were investigated. Both medication and herbal prescription records from a 3-month period were collected for all patients diagnosed with BSS by both DKMs. Results： A total of 169 of 204（82.8%） patients received consistent diagnosis related to BSS by two DKMs. Among them, 54.4%（92 cases） were diagnosed with BSS, and 45.6%（77 cases） were not diagnosed with BSS. DKMs most frequently cited symptoms of recent traumatic injury as justifications for BSS diagnoses, and also selected pain-related indicators such as abdominal pain, sharp pain and nocturnal pain as important reasons in diagnosing BSS. In addition, an inconsistency in the pattern identification theory with respect to traumatic injury was observed. Although only 92 cases（54.4%） of patients were diagnosed with BSS, 77.6% of them were prescribed decoctions for BSS. Conclusions： DKMs considered traumatic injury could cause BSS, and utilized decoction for BSS in patients with traumatic injury without confirming a diagnosis of BSS because they assumed the main symptoms or pathologies of traumatic injury to be closely related to BSS.

关键词： blood stasis syndrome Korean medicine traumatic injury herbal medicine diagnosis pattern identification

Chinese Medicines Reported to Have Effects on Contact Dermatitis in the Last 20 Years

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Chinese Journal of Integrative Medicine 2018年第1期24卷 64-71页

作者： Youngchul jung Byungju Kim mi Heon Ryu Hyungwoo Kim School of Korean Medicine Pusan National University School of Dentistry Pusan National University

Contact dermatitis （CD） is one of the most common skin diseases in industrialized countries. Chinese medicines （CMs） have been investigated worldwide as complementary and alternative medicines for corticosteroids, which are the first choice for treatment of inflammatory skin diseases owing to their favorable efficacy. This article describes the CMs that have been reported to have anti-dermatitis effects against CD in the last 20 years.

关键词： contact dermatitis herbal Chinese medicine

Fuzzy logic controller design with unevenly-distributed membership function for high performance chamber cooling system

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Journal of Central South University 2014年第7期21卷 2684-2692页

作者：曹健鹏 Seok-Kwon Jeong Young-mi jung Graduate School of Refrigeration and Air-conditioning Engineering Pukyong National University Department of Refrigeration and Air-conditioning Engineering Pukyong National University

Fuzzy logic controller adopting unevenly-distributed membership function was presented with the purpose of enhancing performance of the temperature control precision and robustness for the chamber cooling *** equalization and noise detection were performed to modify the evenly-distributed membership functions of error and error change rate into unevenly-distributed membership ***,the experimental results with evenly and unevenly distributed membership functions were compared under the same outside environment *** experimental results show that the steady-state error is reduced around 40% and the noise disturbance is rejected successfully even though noise range is 60% of the control precision *** control precision is improved by reducing the steady-state error and the robustness is enhanced by rejecting noise disturbance through the fuzzy logic controller with unevenly-distributed membership ***,the system energy efficiency and lifetime of electronic expansion valve(EEV) installed in chamber cooling system are improved by adopting the unevenly-distributed membership function.

关键词： chamber cooling system fuzzy logic controller unevenly-distributed membership function steady-state error reduction robustness variable speed refrigeration system

Diagnostic Indicators for Blood Stasis Syndrome Patients with Gynaecological Diseases

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Chinese Journal of Integrative Medicine 2018年第10期24卷 752-757页

作者： Jeeyoun jung mi mi Ko Myeong Soo Lee So min Lee Ju Ah Lee Korea Medicine Fundamental Research Division Korea Institute of Oriental Medicine Clinical Research Division Korea Institute of Oriental Medicine

Objective： To investigate the important diagnostic indicators for blood stasis syndrome （BSS） in patients of childbearing age with gynaecological diseases. Methods： A partial least squared-discriminant analysis （PLS-DA） were applied to BSS symptoms data of patients with gynaecological diseases, and the diagnostic indicators used by doctors of Korean medicine （DKMs） among BSS patients with gynaecological diseases were also investigated. Results： A total of 103 patients of childbearing age with gynaecological diseases and 40 healthy controls were enrolled. Among the 103 patients, 63 （61.7%） and 40 （38.8%） were diagnosed with BSS and non-BSS, respectively, and BSS patients exhibited a more severe extent of disease. A score plot of PLS-DA showed clearly different patterns among the 3 groups. Based on the variable importance on projection of PLS-DA model, menstrual pains, dark lumps in the menstrual blood, ileocoecal tenderness and resistance, sharp pains, and sublingual varicosities were selected as the top five most important indicators. Moreover, more than 75% of DKMs chose dark lumps in menstrual blood, menstrual pain, and dark menstrual blood as the diagnostic indicators of BSS in patients with gynaecological diseases, and more than 49% of them also considered sharp pains, dark red tongue, sublingual varicosities, and tendency to bruise easily as diagnostic indicators of BSS. Conclusion： DKMs focused on menstrual symptoms and certain gynaecological symptoms to diagnose BSS patients of childbearing age with female diseases.

关键词： blood stasis syndrome diagnosis gynaecological disease pattern identification

Breaking out from the neuroprotective logjam:combined treatment with remote ischemic conditioning and minocycline in the prehospital setting

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Neural Regeneration Research 2015年第4期10卷 537-539页

作者： jung mi Park David C.Hess Departments of Neurology Georgia Regent’s University

The only two treatments effective for acute ischemic stroke are reperfusion therapies. Despite testing of hundreds of neuroprotective agents and treatments, tissue plasminogen activator （tPA）, approved by the Food and Drug Administration （FDA） in 1996, remains the only FDA-approved drug for the treatment of ischemic stroke. Recently,

关键词： Breaking out from the neuroprotective logjam RIC

CRISPR-Mediated Engineering across the Central Dogma in Plant Biology for Basic Research and Crop Improvement

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Molecular Plant 2021年第1期14卷 127-150页

作者： Dibyajyoti Pramanik Rahul Mahadev Shelake mi jung Kim Jae-Yean Kim Division of Applied Life Science(BK21 Four Program) Plant Molecular Biology and Biotechnology Research CenterGyeongsang National UniversityJinju 52828Korea These authors contributed equally to this article

The central dogma(CD)of molecular biology is the transfer of genetic information from DNA to RNA to *** CD processes governing genetic flow include the cell cycle,DNA replication,chromosome packaging,epigenetic changes,transcription,posttranscriptional alterations,translation,and posttranslational *** CD processes are tightly regulated in plants to maintain genetic integrity throughout the life cycle and to pass genetic materials to next *** of various CD processes involved in gene regulation will accelerate crop improvement to feed the growing world *** technology enables programmable editing of CD processes to alter DNA,RNA,or protein,which would have been impossible in the ***,an overview of recent advancements in CRISPR tool development and CRISPR-based CD modulations that expedite basic and applied plant research is ***,CRISPR applications in major thriving areas of research,such as gene discovery(allele mining and cryptic gene activation),introgression(de novo domestication and haploid induction),and application of desired traits beneficial to farmers or consumers(biotic/abiotic stress-resilient crops,plant cell factories,and delayed senescence),are ***,the global regulatory policies,challenges,and prospects for CRISPR-mediated crop improvement are discussed.

关键词： central dogma CRISPR crop improvement gene regulation genome editing

Effect of supercooling storage applied with stepwise algorithm for fishes(salmon and olive flounder)and its freshness during extended storage

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Food Bioscience 2022年第5期49卷 774-782页

作者： Dong Hyeon Park SangYoon Lee Yeong mi Byeon Eun Jeong Kim mi-jung Choi Department of Food Science and Biotechnology of Animal Resources Konkuk UniversitySeoul05029South Korea Refrigerator Research of Engineering Division Home Appliance and Air Solution CompanyLG ElectronicsChangwon51533South Korea

Supercooling storage reduces the temperature of a product by lowering its freezing point without phase transition and may extend its shelf ***,it is difficult to maintain the supercooled state of food as it is thermodynamically metastable.A slow cooling rate and minimal fluctuation are essential for achieving stable supercooling ***,a stepwise algorithm was adopted for supercooling storage in this *** and olive flounder were stored at 3℃(refrigeration),􀀀18℃(freezing),and􀀀2℃(supercooling)for 12 *** were maintained in a supercooled state and unfrozen during the storage *** stored after the supercooling treatment were superior with respect to drip loss and water holding capacity(WHC)compared to frozen samples,regardless of the type of *** and total volatile basic nitrogen values of olive flounder was higher than those in salmon owing to the higher water and protein content in olive flounder than in ***,the supercooled samples inhibited the increase in trimethylamine and volatile basic nitrogen *** growth was ***,a stepwise algorithm for stable supercooled storage was achieved,which effectively preserved fish quality better than freezing and refrigeration storage.

关键词： Salmon Olive flounder Supercooling Stepwise algorithm Freshness