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International Journal of Modeling, Simulation, and Scientific Computing 2020年第1期11卷 42-57页

作者： Lei Zhang Chaofeng Zhang mengya liu School of Physics and Electronics Henan University KaifengHenan 475004P.R.China

According to the relationship between truncation error and step size of two implicit second-order-derivative multistep formulas based on Hermite interpolation polynomial,a variable-order and variable-step-size numerical method for solving differential equations is *** stability properties of the formulas are discussed and the stability regions are *** deduced methods are applied to a simulation *** results show that the numerical method can satisfy calculation accuracy,reduce the number of calculation steps and accelerate calculation speed.

关键词： Numerical method variable step size variable order hermite interpolation ordinary differential equations

Blastoids generated purely from embryonic stem cells both in mice and humans

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Science China(Life Sciences) 2024年第2期67卷 418-420页

作者： Baojiang Wu Yanqiu Wang Jia Yan mengya liu Xihe Li Fuchou Tang Siqin Bao The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock Inner Mongolia UniversityHohhot 010020China Research Center for Animal Genetic Resources of Mongolia Plateau College of Life SciencesInner Mongolia UniversityHohhot 010020China Beijing Advanced Innovation Center for Genomics and Biomedical Pioneering Innovation Center College of Life SciencesPeking UniversityBeijing 100871China Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal Hohhot 011517China

Dear Editor,Embryonic stem cells(ESCs)and trophoblast stem cells(TSCs)are derived from blastocysts(Lu et al.,2001).Blastocyst-like structures(blastoids)are self-assembled structures formed by a combination of ESCs and TSCs(Rivron et al.,2018).To increase the success rate of blastoid formation and its similarity with the features of blastocysts,some reports showed that blastoid could also be derived from a combination of three cell lines,ESCs,TSCs,and extraembryonic endoderm cells(XENs),or novel type of stem cells(Sozen et al.,2018;Weatherbee et al.,2023:Wu et al.,2023;Zhang et al.,2023;Zhang et al.,2019).

关键词： purely similarity

Integrative analysis of transcriptome, DNA methylome, and chromatin accessibility reveals candidate therapeutic targets in hypertrophic cardiomyopathy

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Protein & Cell 2024年第11期15卷 796-817页

作者： Junpeng Gao mengya liu Minjie Lu Yuxuan Zheng Yan Wang Jingwei Yang Xiaohui Xue Yun liu Fuchou Tang Shuiyun Wang Lei Song Lu Wen Jizheng Wang Biomedical Pioneering Innovation Center School of Life Sciences Peking University Emergency Center Zhongnan Hospital of Wuhan University Beijing Advanced Innovation Center for Genomics (ICG) Ministry of Education Key Laboratory of Cell Proliferation and Differentiation State Key Laboratory of Cardiovascular Disease Fuwai Hospital National Center for Cardiovascular Disease Chinese Academy of Medical Science and Peking Union Medical College Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Department of Cardiovascular Surgery Fuwai Hospital National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Cardiomyopathy Ward Fuwai Hospital National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College National Clinical Research Center for Cardiovascular Diseases Fuwai Hospital National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College

Hypertrophic cardiomyopathy(HCM) is the most common inherited heart disease and is characterized by primary left ventricular hypertrophy usually caused by mutations in sarcomere genes. The mechanism underlying cardiac remodeling in HCM remains incompletely understood. An investigation of HCM through integrative analysis at multi-omics levels will be helpful for treating HCM. DNA methylation and chromatin accessibility, as well as gene expression, were assessed by nucleosome occupancy and methylome sequencing(NOMe-seq) and RNA-seq, respectively, using the cardiac tissues of HCM patients. Compared with those of the controls, the transcriptome, DNA methylome, and chromatin accessibility of the HCM myocardium showed multifaceted differences. At the transcriptome level, HCM hearts returned to the fetal gene program through decreased sarcomeric and metabolic gene expression and increased extracellular matrix gene expression. In the DNA methylome, hypermethylated and hypomethylated differentially methylated regions were identified in HCM. At the chromatin accessibility level, HCM hearts showed changes in different genome elements. Several transcription factors, including SP1 and EGR1, exhibited a fetal-like pattern of binding motifs in nucleosome-depleted regions in HCM. In particular, the inhibition of SP1 or EGR1 in an HCM mouse model harboring sarcomere mutations markedly alleviated the HCM phenotype of the mutant mice and reversed fetal gene reprogramming. Overall, this study not only provides a high-precision multi-omics map of HCM heart tissue but also sheds light on the therapeutic strategy by intervening in the fetal gene reprogramming in HCM.

关键词： hypertrophic cardiomyopathy multi-omics chromatin accessibility DNA methylation fetal gene reprogramming therapy

Cell Morphology Modified by Biomaterial Induces miR-21 Expression and Apoptosis

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Journal of Biosciences and Medicines 2016年第12期4卷 42-48页

作者： mengya liu Shuangying Gu Yue Zhou School of Biomedical Engineering and Med-X Research Institution Shanghai Jiao Tong University Shanghai China

Biochemical factors can play an important role in regulating gene expression in human umbilical vein endothelial cells (HUVECs), yet the role of biophysical factors during this process is unknown. Here, we show that physical cues, in the form of parallel microgrooves on the surface of cell adhesive substrates, can change the morphology of HUVECs as well as specific microRNA expression. Cells cultured on microgrooved poly (dimethyl siloxane) (PDMS) surface exhibited a more elongated morphology relative to those cultured on flat surfaces, and favored outgrowth along the axis of groove alignment. The level of microRNAs in the cell was screened by miRNA microchip and verified by qRT-PCR. The result showed that around 26 microRNAs have been modified significantly, among which miR-21 level was dramatically elevated. Western-blotting analysis demonstrated that PTEN, a target of miR- 21, was up-regulated in HUVECs with elongated morphology. Cell apoptosis level was significantly decreased, with was associated with the increasing of miR-21 level. These results suggested that biophysical factors can directly modify HUVECs morphology, thus induce miR-21 expression in HUVECs and its downstream biological functions such as decreasing apoptosis. This study provided evidence that surface microtopology should also be considered in designing biomaterials in tissue engineering application.

关键词： HUVECs Microgroove Topography microRNA Apoptosis

Microtopography Attenuates Endothelial Cell Proliferation by Regulating MicroRNAs

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Journal of Biomaterials and Nanobiotechnology 2017年第3期8卷 189-201页

作者： Dan Wang mengya liu Shuangying Gu Yue Zhou Song Li School of Biomedical Engineering and Med-X Research Institution Shanghai Jiao Tong University Shanghai China Department of Bioengineering and Department of Medicine University of California Los Angeles CA USA

Endothelial cell (EC) morphology can be regulated by the micro/nano topography in engineered vascular grafts and by hemodynamic forces in the native blood vessels. However, how EC morphology affects miRNA and thus EC functions is not well understood. In this study, we addressed this question by using human umbilical vein endothelial cells (HUVECs) cultured on microgrooves as a model. HUVECs were grown on either microgrooved (with 10 μm width/spacing and 3 μm depth) or smooth surfaces. HUVECs on microgrooved surface had elongated and bipolar morphology, while HUVECs on smooth surface showed cobble stone shape or non-polar morphology. EdU staining indicated that HUVECs with elongated morphology had lower proliferation rate compared to their counterpart cultured on smooth surface. Quantitative PCR analysis demonstrated that the expression of the specific microRNAs (miR-10a, miR-19a, miR-221) that targeted proliferation-related genes was all up-regulated. Consistently, the mRNA levels of their respective target genes, mitogen-activated protein kinase kinase kinase 7, Cyclin D1 and c-kit were significantly reduced by a fold change of 0.12 ± 0.01 (p < 0.01), 0.70 ± 0.23 (p 0.05) and 0.76 ± 0.21 (p < 0.05). Other miRNAs such as miR-126 and miR-181a were up-regulated as well, leading to the repression of their targets vascular cell adhesion molecule-1 and prospero homeobox-1. Our results suggested that microgrooved surface may regulate microRNA levels and thus EC functions. These results provide insight into the modulation of EC functions by microtopographic cues, and will facilitate the rational design of microstructured materials for cell and tissue engineering.

关键词： Microgroove PDMS MicroRNA Endothelial Cell

虚拟现实与超越现实:影像、智能及未来——“2023第四届数字媒体艺术与科技国际大会”综述

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当代电影 2023年第8期 173-176页

作者：刘梦雅北京电影学院数字媒体学院

在信息化、数字化日益发达的21世纪,由数字媒体技术构建起的新型社会体系的数字生活空间,映射现实世界也超越现实世界。在此背景下,探索和创新数字媒体艺术与科技的理论、实践及教育模式,以推动中国数字文化产业的高质量发展,显得尤为... 详细信息

在信息化、数字化日益发达的21世纪,由数字媒体技术构建起的新型社会体系的数字生活空间,映射现实世界也超越现实世界。在此背景下,探索和创新数字媒体艺术与科技的理论、实践及教育模式,以推动中国数字文化产业的高质量发展,显得尤为重要。这不仅是新时代中国特色社会主义文化发展的需要,也是构建以人为本,创新富有活力的数字文化强国的必然选择。

关键词：新时代中国特色社会主义数字媒体技术数字文化产业虚拟现实数字媒体艺术文化强国探索和创新信息化

3D电影的空间思维与设计——以徐克3D电影为例

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装饰 2015年第5期 34-37页

作者：刘梦雅北京电影学院

3D电影凭借特有的视听体验优势已经逐渐成为电影展示传播的重要表现力量。电影本是一门时空复合的视听艺术,3D技术作用带来极致的空间表达,快速拉近了电影与观众的距离,由"观看"转向为"体验"。本文以徐克3D电影为例着重探讨了将3D思维... 详细信息

3D电影凭借特有的视听体验优势已经逐渐成为电影展示传播的重要表现力量。电影本是一门时空复合的视听艺术,3D技术作用带来极致的空间表达,快速拉近了电影与观众的距离,由"观看"转向为"体验"。本文以徐克3D电影为例着重探讨了将3D思维作用于电影空间设计的重要性,重点探索了如何从3D电影空间设计之初,就开始将3D思维应用其中,并贯穿始终,从而营造更为逼真的空间体验,让观众毫不勉强地沉浸其中、流连忘返。

关键词： 3D电影 3D思维空间设计徐克

2020-2021年春节档国产动画电影的发展研究

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电影评介 2021年第5期 88-93页

作者：刘梦雅北京电影学院数字媒体学院

一、数据解读:2020-2021年春节档期中国动画电影先抑后扬(一)2020年中国动画电影市场整体降温1.规模缩减,作品上映数量与总票房均回落2020年,历经178天的院线空窗期,全年动画电影共上映20部,纵观2016-2020年全国动画电影上映数量(表1),... 详细信息

一、数据解读:2020-2021年春节档期中国动画电影先抑后扬(一)2020年中国动画电影市场整体降温1.规模缩减,作品上映数量与总票房均回落2020年,历经178天的院线空窗期,全年动画电影共上映20部,纵观2016-2020年全国动画电影上映数量(表1),与2019年的73部相较,减少了72.6%,与2018年的61部相较减少了67.2%。

关键词：总票房中国动画电影国产动画电影院线春节档空窗期档期数据解读

镍基高温合金表面冲击强化机制及应用研究进展

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金属学报 2023年第9期59卷 1173-1189页

作者：王磊刘梦雅刘杨宋秀孟凡强东北大学材料各向异性与织构教育部重点实验室沈阳110819 中山大学中法核工程与技术学院珠海519000

为满足不断攀升的两机涡轮动力系统的快速发展,表面冲击强化技术在涡轮转子用高温合金表面强化的应用及相应机制的研究受到了广泛关注。然而,高温合金表面硬化层在高温服役环境下的回复、再结晶行为难以避免,由此引起的表面强韧化、抗... 详细信息

为满足不断攀升的两机涡轮动力系统的快速发展,表面冲击强化技术在涡轮转子用高温合金表面强化的应用及相应机制的研究受到了广泛关注。然而,高温合金表面硬化层在高温服役环境下的回复、再结晶行为难以避免,由此引起的表面强韧化、抗疲劳效果的退化,成为制约表面冲击强化技术在先进高温合金关键部件深入应用的瓶颈。本文总结了近年来镍基高温合金表面冲击强化机制及应用研究进展,分析了表面冲击强化对镍基高温合金表面强韧性及抗疲劳的作用规律,探究了高温合金表面冲击硬化层在高温及长期时效过程中的显微组织、微结构演化及其对高温稳定性的作用机理。以期为发展镍基高温合金表面冲击强化、提高两机涡轮转子疲劳抗力提供基础。

关键词：镍基高温合金表面强化处理抗疲劳制造硬化层组织和性能高温稳定性

穿心莲内酯对神经退行性疾病的作用及其机制研究进展

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中国现代应用药学 2021年第11期38卷 1383-1388页

作者：许杜娟刘萌芽蔡邦荣刘改枝河南中医药大学药学院郑州450046 偃师市中医院河南洛阳471900

氧化应激、线粒体功能障碍以及炎症反应是神经退行性疾病如阿尔兹海默病(Alzheimer’s disease,AD)和帕金森病(Parkinson’s disease,PD)等发生和发展的关键环节。因此,保护线粒体、缓解氧化应激和抑制炎症对防治此类疾病具有重要意义... 详细信息

氧化应激、线粒体功能障碍以及炎症反应是神经退行性疾病如阿尔兹海默病(Alzheimer’s disease,AD)和帕金森病(Parkinson’s disease,PD)等发生和发展的关键环节。因此,保护线粒体、缓解氧化应激和抑制炎症对防治此类疾病具有重要意义。穿心莲内酯是穿心莲的主要有效成分,具有较好的抗炎、抗肿瘤、抗病毒、抗HIV和保肝等生物活性。近年来,越来越多研究证实穿心莲内酯在神经退行性疾病方面有着良好的应用前景,主要通过诱导线粒体自噬而保护线粒体稳态、抑制炎症小体的活化;清除活性氧,保护线粒体,或抑制小胶质细胞炎症反应保护神经元细胞。文章总结了近十年来穿心莲内酯在神经退行性疾病如AD、PD和脑缺血损伤等方面的药理活性及机制研究,以期为穿心莲内酯的研究开发和在神经退行性疾病中的临床应用提供参考。

关键词：穿心莲内酯神经退行性疾病药理活性