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Characterization and comparison of two C-type lectins with novel key motifs from mud crab Scylla paramamosain

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Journal of Oceanology and Limnology 2022年第4期40卷 1628-1639页

作者： Boxin ZENG Taiwei DONG Yanting XIA Shun YANG mengmeng huang Hui FEI College of Life Sciences and Medicine Zhejiang Sci-Tech UniversityHangzhou 310018China Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine Zhejiang Sci-Tech UniversityHangzhou 310018China

As an important pattern recognition receptor(PRR)in the innate immune system,C-type lectin plays an important role in the innate immune process of invertebrates.Two C-type lectins Sp CTL-C and Sp CTL-D were characterized from mud crab(Scylla paramamosain).The predicted Sp CTL-C and Sp CTL-D proteins both contain a single carbohydrate-recognition domain(CRD)with key motif Gln-Pro-Ala(QPA)and Met-Pro-Ala(MPA),respectively.Sp CTL-C and Sp CTL-D transcripts distributed in all examined tissues,and the expression level was the highest in hepatopancreas.As PRR,the purifi ed recombinant proteins r Sp CTL-C and r Sp CTL-D have high affi nity for three kinds of pathogen-associated molecular patterns(PAMPs):β-glucan,lipopolysaccharide,and peptidoglycan.Besides,r Sp CTL-D can bind to all nine microorganisms tested,while r Sp CTL-C can bind to seven microorganisms except for Staphylococcus aureus and Micrococcus luteus.Both r Sp CTL-C and r Sp CTL-D showed agglutination activity towards fungi Pichia pastoris and Saccharomyces cerevisiae.However,r Sp CTL-C and r Sp CTL-D exhibited diff erent antimicrobial activities:r Sp CTL-D has a certain inhibitory eff ect on the growth of Vibrio fl uvialis and M.luteus,while r Sp CTL-C has no obvious inhibitory activity.The results show that r Sp CTL-C and r Sp CTL-D had better phagocytosis-promoting eff ect on M.luteus than the negative control.Meanwhile,both r Sp CTL-C and r Sp CTL-D had certain encapsulation-promoting activity.Collectively,two C-type lectins with novel key motifs make an important impact as PRR in immune response towards pathogens.At the same time,they play diff erent functions in the innate immunity of mud crab S.paramamosain.

关键词： Scylla paramamosain immune response innate immunity C-type lectin

On-demand assembly of polymeric nanoparticles for longer-blood-circulation and disassembly in tumor for boosting sonodynamic therapy

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Bioactive Materials 2022年第12期7卷 242-253页

作者： Mei Wen Nuo Yu Shiwen Wu mengmeng huang Pu Qiu Qian Ren Meifang Zhu Zhigang Chen State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringDonghua UniversityShanghai201620China

Sonodynamic therapy (SDT) is one of the promising strategies for tumor therapy, but its application is usually hindered by fast clearance in blood-circulation, abnormal tumor microenvironment, and inefficient generation of reactive oxygen species. To solve these problems, we proposed an on-demand assembly-disassembly strategy, where the assembly is favorable for longer-blood-circulation and then the disassembly in tumor is favorable for boosting SDT. Hematoporphyrin monomethyl ether (HMME) as the model of organic sonosensitizers were conjugated with hyaluronic acid (HA). Then HA-HMME was mixed with catalase (CAT) and assembled into polymeric nanoparticles (CAT@HA-HMME NPs) with size of ~80 nm. CAT@HA-HMME NPs exhibit good biocompatibility and a longer blood half-time (t1/2 = 4.17 h) which is obviously longer than that (~0.82 h) of HMME molecules. After HA receptor-mediated endocytosis of cancer cells, CAT@HA-HMME NPs can be cleaved by endogenous hyaluronidase, resulting in the on-demand disassembly in tumor to release HA-HMME molecules and CAT. The CAT catalyzes the endogenous H_(2)O_(2) into O_(2) to relieve the hypoxic microenvironment, and the released HA-HMME exhibits a higher ROS generation ability, greatly boosting SDT for the inhibition of tumor growth. Therefore, the on-demand assembly-disassembly strategy may provide some insight in the design and development of nanoagents for tumor therapy.

关键词： Polymeric nanoparticles On-demand assembly Longer-blood-circulation On-demand disassembly Sonodynamic therapy

德国综合安全观:理论缘起、历史演进与实践评估

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国际安全研究 2024年第1期42卷 108-135,165,166页

作者：黄萌萌中国社会科学院欧洲研究所北京100732

德国首份《国家安全战略》以“综合安全”为要义,具有理论逻辑和历史脉络可循。德国综合安全观兴起于冷战后,其内在逻辑是对欧洲主义安全观的继承性发展,聚焦两极秩序瓦解后的经济与社会秩序稳定以及应对域外冲突,注重非传统安全,安全... 详细信息

德国首份《国家安全战略》以“综合安全”为要义,具有理论逻辑和历史脉络可循。德国综合安全观兴起于冷战后,其内在逻辑是对欧洲主义安全观的继承性发展,聚焦两极秩序瓦解后的经济与社会秩序稳定以及应对域外冲突,注重非传统安全,安全参与者包含国家与非国家行为体。克里米亚危机后,传统军事威胁回归欧陆,德国综合安全观“防御性”与“韧性”融合并重。俄乌冲突冲击欧洲安全秩序,德国综合安全观愈发重视国家与联盟防御,并通过“多元化”与“联盟政治”等手段推进“去风险”策略,增强经济与社会韧性,同时也呈现出泛安全化、价值观化与权力政治的特质,其影响力辐射至“印太”地区与对华政策上。然而,德国综合安全政策实践仍面临诸多挑战。防御上,德国提升国防的政治承诺与其军事资源尚不匹配。韧性上,德国“去风险”策略受国际权力对比变化、中国市场红利、德国政界与商界意见的异质性以及德国国内经济成本的制约。为此,中国应客观看待德国安全政策转型的影响。

关键词：综合安全观德国国家安全战略经济去风险德国安全政策转型

Protective efficacy evaluation of immunogenic protein AHA_3793 of Aeromonas hydrophila as vaccine candidate for largemouth bass Micropterus salmoides

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Journal of Oceanology and Limnology 2023年第1期41卷 392-400页

作者： Shun YANG Idefonce MKINGULE Long LIU Wenqi CHEN Xiangyu YUAN Zixuan MA Liang LIANG Shichao QIAN mengmeng huang Hui FEI College of Life Sciences and Medicine Zhejiang Sci-Tech UniversityHangzhou 310018China Zhejiang Development&Planning Institute Hangzhou 310012China Huzhou Baijiayu Biotech Co. Ltd.Huzhou 313000China

Aeromonas hydrophila is a Gram-negative pathogen that can infect various fish,including largemouth bass(Micropterus salmoides),which have caused huge economic losses.In present study,largemouth bass anti-A.hydrophila antibodies were produced,then a highly immunogenic outer membrane proteins,AHA_3793,was identified by combined western blotting and mass spectrometry analysis.Moreover,AHA_3793 was expressed,and its immunogenicity was further verified by western blotting.Subsequently,the protective efficacy of AHA_3793 were evaluated in largemouth bass.The results showed that rAHA_3793 could produce a relative percentage survival(RPS)of 61.76% for largemouth bass against A.hydrophila challenge.ELISA analysis showed the specific serum antibodies of largemouth bass against rAHA_3793 and A.hydrophila in vaccinated group in weeks 4 and 5 after immunization were significantly higher than those in control group,which suggested that rAHA_3793 induced production of specific serum antibodies against rAHA_3793 and A.hydrophila.The qRT-PCR analysis showed that expressions of CD4-2 and MHC Ⅱα were also significantly up-regulated after immunization.These results collectively demonstrated that rAHA_3793 could induce a strong humoral immune response of largemouth bass,and then produce high immune protection ef fects against A.hydrophila infection.

关键词： Micropterus salmoides Aeromonas hydrophila AHA_3793 subunit vaccine immune response

缩口T型微通道内纳米流体吸收CO_(2)的流动与传质研究

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化工学报 2024年第1期75卷 221-230页

作者：赵若晗黄蒙蒙朱春英付涛涛高习群马友光天津大学化工学院化学工程联合国家重点实验室天津300072 辽阳石油化纤公司亿方工业公司辽宁辽阳111003

构建了一种气相缩口的T型微通道,研究了二氧化硅(SiO_(2))纳米流体吸收CO_(2)过程的气液两相流动与传质性能。在实验范围内,观察到了泡状流、串珠流、紧密弹状流和弹状-环状流。随着气相流速的增加,泡状流的气泡生成频率f和比表面积a快... 详细信息

构建了一种气相缩口的T型微通道,研究了二氧化硅(SiO_(2))纳米流体吸收CO_(2)过程的气液两相流动与传质性能。在实验范围内,观察到了泡状流、串珠流、紧密弹状流和弹状-环状流。随着气相流速的增加,泡状流的气泡生成频率f和比表面积a快速增大,串珠流的f和a变化很小,紧密弹状流的f和a逐渐减小。随着连续相和分散相流速的增大以及纳米颗粒浓度的升高,液侧体积传质系数均表现出增大的趋势。与等宽T型通道相比,缩口T型微通道的最大比表面积增幅达29.6%。结果表明气相入口的缩径效应可有效提高气液两相流的传质面积,有利于气液传质性能的改善和提高。

关键词：二氧化碳捕集纳米流体微通道传质过程强化

盐酸西替利嗪滴剂联合丙酸氟替卡松吸入气雾剂治疗儿童过敏性鼻炎合并支气管哮喘疗效观察

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儿科药学杂志 2024年第2期30卷 28-31页

作者：黄萌萌董亮亮王红梅聊城市第二人民医院山东聊城252600

目的:观察盐酸西替利嗪滴剂联合丙酸氟替卡松吸入气雾剂治疗过敏性鼻炎(AR)合并支气管哮喘(BA)患儿的疗效及安全性。方法:选取2020年1月-2022年6月我院收治的AR合并BA患儿84例,按随机数表法分为联合组与对照组各42例。对照组给予丙酸氟... 详细信息

目的:观察盐酸西替利嗪滴剂联合丙酸氟替卡松吸入气雾剂治疗过敏性鼻炎(AR)合并支气管哮喘(BA)患儿的疗效及安全性。方法:选取2020年1月-2022年6月我院收治的AR合并BA患儿84例,按随机数表法分为联合组与对照组各42例。对照组给予丙酸氟替卡松吸入气雾剂吸入治疗,联合组则采用盐酸西替利嗪滴剂联合丙酸氟替卡松吸入气雾剂治疗,比较两组患儿治疗前后AR和BA严重程度评分、肺功能指标[第1秒用力呼气量(FEV1)、呼气流速(PEF)、用力肺活量(FVC)]、血清酶学[可溶性细胞间粘附分子-1(sICAM-1)、可溶性血管细胞间粘附分子-1(sVCAM-1)、白细胞介素-4(IL-4)、IL-10]、血清淀粉样蛋白(SAA)水平及不良反应发生情况。结果:治疗后,联合组AR与BA严重程度评分低于对照组,FEV1、PEF及FVC水平高于对照组(P均0.05)。结论:采用盐酸西替利嗪滴剂联合丙酸氟替卡松吸入气雾剂治疗AR合并BA患儿,能显著减轻临床症状,有效改善肺功能,调节血清酶学指标与SAA水平,且不会增加不良反应的发生。

关键词：盐酸西替利嗪滴剂丙酸氟替卡松过敏性鼻炎支气管哮喘血清酶学血清淀粉样蛋白

Predicting the International Roughness Index of JPCP and CRCP Rigid Pavement:A Random Forest(RF)Model Hybridized with Modified Beetle Antennae Search(MBAS)for Higher Accuracy

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Computer Modeling in Engineering & Sciences 2024年第5期139卷 1557-1582页

作者： Zhou Ji mengmeng Zhou Qiang Wang Jiandong huang College of Civil and Environmental Engineering Hunan University of Science and EngineeringYongzhou425006China School of Mines China University of Mining and TechnologyXuzhou221116China School of Civil Engineering Guangzhou UniversityGuangzhou510006China

To improve the prediction accuracy of the International Roughness Index(IRI)of Jointed PlainConcrete Pavements(JPCP)and Continuously Reinforced Concrete Pavements(CRCP),a machine learning approach is developed in this study for the modelling,combining an improved Beetle Antennae Search(MBAS)algorithm and Random Forest(RF)model.The 10-fold cross-validation was applied to verify the reliability and accuracy of the model proposed in this study.The importance scores of all input variables on the IRI of JPCP and CRCP were analysed as well.The results by the comparative analysis showed the prediction accuracy of the IRI of the newly developed MBAS and RF hybrid machine learning model(RF-MBAS)in this study is higher,indicated by the RMSE and R values of 0.2732 and 0.9476 for the JPCP as well as the RMSE and R values of 0.1863 and 0.9182 for the CRCP.The accuracy of this obtained result far exceeds that of the IRI prediction model used in the traditional Mechanistic-Empirical Pavement Design Guide(MEPDG),indicating the great potential of this developed model.The importance analysis showed that the IRI of JPCP and CRCP was proportional to the corresponding input variables in this study,including the total joint faulting cumulated per KM(TFAULT),percent subgrade material passing the 0.075-mm Sieve(P_(200))and pavement surface area with flexible and rigid patching(all Severities)(PATCH)which scored higher.

关键词： Cement pavement JPCP CRCP RF-MBAS IRI

不同时机超声引导腹直肌鞘阻滞对术后镇痛的影响

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中国现代医生 2024年第15期62卷 59-62,78页

作者：付品国李兴旺杨建军黄梦朦温州医科大学附属第二医院(育英儿童医院)麻醉与围术期医学科、小儿麻醉学教育部重点实验室、浙江省麻醉学重点实验室浙江温州325000

目的探讨单孔腹腔镜妇科手术超声引导腹直肌鞘阻滞的最佳时机。方法选取2021年8月至2022年1月温州医科大学附属第二医院妇科病房63例择期行单孔腹腔镜下妇科良性肿物切除手术的患者为研究对象,根据随机数字表法将其分为E组(32例)和O组(3... 详细信息

目的探讨单孔腹腔镜妇科手术超声引导腹直肌鞘阻滞的最佳时机。方法选取2021年8月至2022年1月温州医科大学附属第二医院妇科病房63例择期行单孔腹腔镜下妇科良性肿物切除手术的患者为研究对象,根据随机数字表法将其分为E组(32例)和O组(31例),剔除3例,最终纳入60例,每组各30例。E组患者采用术前超声引导腹直肌鞘阻滞,O组患者采用术后超声引导腹直肌鞘阻滞。比较两组患者拔管后即刻(T_(1))、术后12h(T_(2))、术后24h(T_(3))静息、活动状态的数字疼痛量表(numerical rating scale,NRS)评分;比较两组患者术中切皮前后的平均动脉压及心率;比较两组患者术中瑞芬太尼使用量、术后羟考酮使用量、术后镇痛补救率;比较两组患者入室时长及手术时长;记录两组患者神经阻滞并发症的发生情况。结果整体分析发现:两组患者静息NRS评分时间点比较及交互作用差异均有统计学意义(P<0.05),活动NRS评分时间点比较差异有统计学意义(P<0.05)。进一步两两比较,组内比较:O组患者T_(2)、T_(3)时点静息NRS和活动NRS评分均显著低于T_(1)时点(P<0.05);E组患者T_(3)时点静息NRS评分显著低于T_(1)时点,T_(2)、T_(3)时点活动NRS评分均显著低于T_(1)时点(P<0.05)。组间比较:E组患者T_(1)时点静息NRS和活动NRS评分均显著低于O组(P<0.05)。切皮后,O组患者的平均动脉压显著高于本组切皮前,心率显著快于本组切皮前(P<0.05)。两组均无腹腔穿刺、穿刺点感染、局部麻醉药中毒等情况发生。结论单孔腹腔镜妇科手术中,术前超声引导下腹直肌鞘神经阻滞,在术后早期可为患者提供更佳的镇痛效果,且患者术中切皮前后心率和平均动脉压变化更小,值得推荐。

关键词：单孔腹腔镜手术腹直肌鞘阻滞超前镇痛术后镇痛

Analysis of Calcined Red Mud Properties and Related Mortar Performances

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Fluid Dynamics & Materials Processing 2024年第5期20卷 901-913页

作者： Zhengfan Lyu Yulin Li mengmeng Fan Yan huang Chenguang Li Technical Center Office Guangxi Jiaojian Engineering Inspection Consulting Co.Ltd.Nanning530001China Technical Center Office Guangxi Road Construction Engineering Group Co.Ltd.Nanning530001China Nanning Road Construction Technology and Road Construction Materials Engineering Technology Research Centre Guangxi Road Construction Engineering Group Co.Ltd.Nanning530001China

Red mud(RM)is a low-activity industrial solid waste,and its utilization as a resource is currently a hot topic.In this study,the micro characteristics of red mud at different calcination temperatures were analyzed using X-ray diffraction and scanning electron microscopy.The performance of calcined red mud was determined through mortar strength tests.Results indicate that high-temperature calcination can change the mineral composition and microstructure of red mud,and increase the surface roughness and specific surface area.At the optimal temperature of 700°C,the addition of calcined red mud still leads to a decrease in mortar strength,but its activity index and flexural coefficient increase by 16.2%and 11.9%with respect to uncalcined red mud,reaching values of 0.826 and 0.974,respectively.Compared with the control group,the synergistic activation of calcined red mud with slag can increase the compressive and flexural strength of the mortar by 12.9%and 1.5%,reaching 8.7 and 62.4 MPa,respectively.Correspondingly,the activity index and flexural coefficient of the calcined RM and GGBS(Ground Granulated Blast furnace Slag)mixtures also increase to 1.015 and 1.130,respectively.

关键词： Red mud slag thermal activation synergistic activation mortar test microscopic properties