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Mechanisms by which fibroblast growth factor 20 improves motor performance in a mouse model of Parkinson’s disease

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Neural Regeneration Research 2019年第8期14卷 1438-1444页

作者： Ai-Qin Wang Li-Na Kong Ming-zhu meng Xiu-He Zhao Si Chen Xiao-Tang Wang Qilu Hospital of Shandong University

Genome-wide studies have reported that Parkinson’s disease is associated with abnormal expression of various growth factors. In this study, male C57 BL/6 mice aged 10 weeks were used to establish Parkinson’s disease models using an intraperitoneal injection of 60 mg/kg 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. 28 days later, 10 or 100 ng fibroblast growth factor 20 was injected intracerebroventricularly. The electrophysiological changes in the mouse hippocampus were recorded using a full-cell patch clamp. Expression of Kv4.2 in the substantia nigra was analyzed using a western blot assay. Serum malondialdehyde levels were analyzed by enzyme-linked immunosorbent assay. The motor coordination of mice was evaluated using the rotarod test. The results showed that fibroblast growth factor 20 decreased A-type potassium current in neurons of the substantia nigra, increased long-term potentiation amplitude in the hippocampus, and downregulated Kv4.2 expression. A high dose of fibroblast growth factor 20 reduced serum malondialdehyde levels and enhanced the motor coordination of mice. These findings confirm that fibroblast growth factor 20 has a therapeutic effect on the toxicity induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, and its mechanism of action is associated with the inhibition of A-type K+ currents and Kv4.2 expression. All animal procedures were approved by the Animal Care and Use Committee of Qilu Hospital of Shandong University, China in 2017(approval No. KYLL-2017-0012).

关键词： nerve regeneration Parkinson’s disease 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine fibroblast growth factor 20 A-type potassium current long-term potentiation Kv4.2 oxidative stress malondialdehyde motor performance neural regeneration

Liver fat accumulation measured by high-speed T2-corrected multiecho magnetic resonance spectroscopy can predict risk of cholelithiasis

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World Journal of Gastroenterology 2020年第33期26卷 4996-5007页

作者： Hong Chen Wei-Ke Zeng Guang-Zi Shi Ming Gao meng-zhu Wang Jun Shen Department of Radiology Sun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhou 510120Guangdong ProvinceChina Department of MR Scientific Marketing Siemens HealthineersGuangzhou 510120Guangdong ProvinceChina

BACKGROUND Liver fat accumulation is associated with increased cholesterol synthesis and hypersecretion of biliary cholesterol,which may be related to the development of *** To investigate whether liver fat accumulation measured by high-speed T2-corrected multi-echo magnetic resonance spectroscopy(MRS)is a risk factor for *** Forty patients with cholelithiasis and thirty-one healthy controls were retrospectively *** participants underwent high-speed T2-corrected multi-echo single-voxel MRS of the liver at a 3T MR *** proton density fat fraction(PDFF)and R2 value were *** parameters and waist circumference(WC)were ***’s correlation analysis was used to analyze the relationship between PDFF,R2,and WC *** logistic regression analysis was carried out to determine the significant predictors of the risk of *** operating characteristic curve(ROC)analysis was used to evaluate the discriminative performance of significant *** Patients with cholelithiasis had higher PDFF,R2,and WC values compared with healthy controls(5.8%±4.2%vs 3.3%±2.4%,P=0.001;50.4±24.8/s vs 38.3±8.8/s,P=0.034;85.3±9.0 cm vs 81.0±6.9 cm,P=0.030;respectively).Liver iron concentration extrapolated from R2 values was significantly higher in the cholelithiasis group(2.21±2.17 mg/g dry tissue vs 1.22±0.49 mg/g dry tissue,P=0.034)than in the healthy *** was positively correlated with WC(r=0.502,P<0.001)and R2(r=0.425,P<0.001).Multivariate logistic regression analysis showed that only PDFF was an independent risk factor for cholelithiasis(odds ratio=1.79,95%CI:1.22-2.62,P=0.003).ROC analysis showed that the area under the curve of PDFF was 0.723 for discriminating cholelithiasis from healthy controls,with a sensitivity of 55.0%and specificity of 83.9%when the cut-off value of PDFF was 4.4%.CONCLUSION PDFF derived from high speed T2-corrected multi-echo MRS can predict the risk o

关键词： Magnetic resonance spectroscopy Cholelithiasis Liver fat accumulation Steatosis Iron

Inhibitive effect of TAK-242 on Tenon’s capsule fibroblasts proliferation in rat eyes

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International Journal of Ophthalmology(English edition) 2019年第11期12卷 1699-1707页

作者： Liang Liang meng-Nan zhu Bao-Ji Chen Zheng Wang Li-Ye He Rang Zhang Department of Ophthalmology The First College of Clinical Medical ScienceChina Three Gorges UniversityYichang 443003Hubei ProvinceChina Department of Ophthalmology Yichang Central People’s HospitalYichang 443003Hubei ProvinceChina Department of Ophthalmology Xianning Central HospitalXianning 437100Hubei ProvinceChina

AIM: To study the inhibition effect of TAK-242 on the proliferation of rat eye Tenon's capsule fibroblasts via the toll-like receptor 4(TLR4) signaling ***: SD rat Tenon's capsule fibroblasts were extracted and cultured, then the cells were divided into normal control group, lipopolysaccharide(LPS) group(10 g/m L LPS) and TAK-242 group(1 μmol/L TAK-242, and 10 μg/m L LPS after 30 min). The expressions of TLR4, transforming growth factor-β1(TGF-β1) and interleukin-6(IL-6) in each group were detected by Western blot and reverse transcriptase-polymerase chain reaction(RT-PCR). Cell proliferation was detected by cell counting kit-8(CCK-8).RESULTS: Double immunofluorescent labeling in the extracted cells showed negative keratin staining and positive vimentin staining. Western blot showed that the LPS group had the highest expression of TLR4 and TGF-β1(P0.05). RT-PCR showed that the IL-6 m RNA expression in LPS group was the highest in the three groups(P<0.01). CONCLUSION: TAK-242 inhibits the proliferation of LPSinduced Tenon's capsule fibroblasts and the release of inflammatory factors by regulating the TLR4 signalingpathway, providing a new idea for reducing the scarring of the filter passage after glaucoma filtration surgery.

关键词： Tenon's capsule fibroblasts fibrosis TAK-242 rat

Development of re-crosslinkable dispersed particle gels for conformance improvement in extremely high-temperature reservoirs

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Petroleum Science 2022年第6期19卷 2922-2931页

作者： Dao-Yi zhu Run-Tian Luo Yang Liu Jun-Hui Qin Qi Zhao Hong-Jun Zhang Wan-Sheng Wang Zi-Yuan Wang meng-En zhu Yi-Peng Wang Peng-Bo Li China University of Petroleum-Beijing at Karamay Karamay 834000XinjiangChina

Micro-scale and nano-scale dispersed gel particles(DPG)are capable of deep migration in oil reservoirs due to their deformability,viscoelasticity,and suitable particle ***,it has been widely studied and applied in reservoir conformance control in recent ***,for highly permeable channels,their plugging performance is still *** addition,conventional in situ cross-linked polymer gels(ISCPGs)have fast gelation time under extremely high-temperature conditions,which often causes problems such as difficulty in ***,a re-cross linkable dispersed particle gel(RDPG)system applied for conformance control in highly permeable channels of extremely high-temperature petroleum reservoirs was *** particle size distribution,gelation time,gel strength,injection performance,and perfo rmance strength in po rous media were investigated using a laser particle size meter,the Sydansk bottle test method,rheometer,and core displacement experiments,*** show that the RDPG suspension can be stable for more than 6 months at room temperature with storage modulus G’much lower than 10 *** can pass through the pore throat by elastic deformation effect and does not cause strong ***,it can undergo re-crosslinking reaction at 150℃to form a strong bulk *** gel strength G’of re-crosslinked RDPG can be as high as 69.3 Pa,which meets the strength requirement of conformance *** RDPG suspension has the properties of easy injection,and it also has strong plugging,and high-temperature resistance after re-crosslinked in the core,which can be a very promising material for conformance improvement in extremely high-temperature reservoirs.

关键词： Dispersed particle gel Re-crosslinking Conformance improvement High-temperature petroleum reservoir Injection performance

Green transformation of manufacturing industry for ecological protection in Jiangsu Province

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Ecological Economy 2017年第4期10卷 363-367页

作者： zhu meng School of Management Yangzhou Polytechnic College

Under the new normal economy, the manufacturing industry is developing rapidly as the focus of economic development in Jiangsu. Its rapid development has brought great opportunities to our society, but at the same time, great challenges has been brought to the natural environment. The resource consumption and environmental pollution problems have become increasingly prominent. In the face of this situation, we must try every means to reduce pollution and save energy. Therefore, the transition trend of manufacturing industry in the future is bound to take the green economy as the background. Thus, this paper analyzes the drawbacks of the manufacturing industry in Jiangsu province. And the status of traditional manufacturing enterprises and advantages of green manufacturing are analyzed. Then, the paper proposed to explore the practice of green manufacturing mode for the ecological protection, so as to achieve the industrial transformation and upgrading of manufacturing industry in Jiangsu province.

关键词： manufacturing industry green manufacturing transformation and upgrading clean production Jiangsu province

3D printed grafts with gradient structures for organized vascular regeneration

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International Journal of Extreme Manufacturing 2024年第3期6卷 505-520页

作者： Yuewei Chen Zhongfei Zou Tao Fu zhuang Li Zhaojie Zhang meng zhu Qing Gao Shaofei Wu Guosheng Fu Yong He Jiayin Fu State Key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China School of Mechanical Engineering Guizhou UniversityGuiyang 550025People’s Republic of China Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province Department of CardiologySir Run Run Shaw HospitalZhejiang UniversityHangzhou 310016People’s Republic of China School of Mechanical Engineering Guizhou Institute of TechnologyGuiyang 550003People’s Republic of China Department of Oral and Maxillofacial Surgery The Second Affiliated Hospital of Zhejiang University School of MedicineSchool of Stomatologyand Key Laboratory of Oral Biomedical Research of Zhejiang ProvinceHangzhouZhejiang 310006People’s Republic of China

Synthetic vascular grafts suitable for small-diameter arteries(<6 mm) are in great ***,there are still no commercially available small-diameter vascular grafts(SDVGs) in clinical practice due to thrombosis and stenosis after in vivo *** designing SDVGs,many studies emphasized reendothelization but ignored the importance of reconstruction of the smooth muscle layer(SML).To facilitate rapid SML regeneration,a high-resolution 3D printing method was used to create a novel bilayer SDVG with structures and mechanical properties mimicking natural *** by the collagen alignment of SML,the inner layer of the grafts had larger pore sizes and high porosity to accelerate the infiltration of cells and their circumferential alignment,which could facilitate SML reconstruction for compliance restoration and spontaneous *** outer layer was designed to induce fibroblast recruitment by low porosity and minor pore size and provide SDVG with sufficient mechanical *** month after implantation,the arteries regenerated by 3D-printed grafts exhibited better pulsatility than electrospun grafts,with a compliance(8.9%) approaching that of natural arteries(11.36%) and significantly higher than that of electrospun ones(1.9%).The 3D-printed vascular demonstrated a three-layer structure more closely resembling natural arteries while electrospun grafts showed incomplete endothelium and immature *** study shows the importance of SML reconstruction during vascular graft regeneration and provides an effective strategy to reconstruct blood vessels through 3D-printed structures rapidly.

关键词： small-diameter vascular graft smooth muscle layer 3D printing endothelialization

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利用虚光子和康普顿散射对同步辐射的分析

强激光与粒子束 2005年第3期17卷 477-480页

作者：孟现柱聊城大学物理科学与信息工程学院山东聊城252059

从虚光子的康普顿散射出发,给出了一种对同步辐射进行解释的新理论。当带电粒子在加速器中做圆周运动时,加速器的磁场可以看作周期静磁场,可以等效为虚光子。当该虚光子与加速器中运动的带电粒子发生康普顿散射时,就会转换为实光子辐射... 详细信息

从虚光子的康普顿散射出发,给出了一种对同步辐射进行解释的新理论。当带电粒子在加速器中做圆周运动时,加速器的磁场可以看作周期静磁场,可以等效为虚光子。当该虚光子与加速器中运动的带电粒子发生康普顿散射时,就会转换为实光子辐射出去。给出了同步辐射实光子的波长的计算公式,并从合肥国家同步实验室和北京同步辐射装置的峰值特征波长以及斯坦福直线加速器中心的Ec/E3e 值、强磁场中子星极区的同步辐射等方面验证了该公式的正确性。

关键词：高能物理虚光子实光子康普顿散射同步辐射

UPLCeMS/MS for the determination of azilsartan in beagle dog plasma and its application in a pharmacokinetics study

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Asian Journal of Pharmaceutical Sciences 2015年第3期10卷 247-253页

作者： Cheng Gong Junfeng Wang Yinghua Sun Dawei Ding Lu Zhong meng zhu Jin Sun Xiangrong Zhang Shenyang Pharmaceutical University No.103Wenhua RoadShenyang 110016China Shandong Weigao Pharm.Co.Ltd. Weihai 264200China

The purpose of the study is to develop an ultra performance liquid chromatographytandem mass spectrometry(UPLCeMS/MS)to determinate the concentration of azilsartan in the dog *** precipitated by methanol,the plasma sample containing azilsartan and diazepam(internal standard,IS)was determined by UPLCeMS/*** mobile phase consisted of acetonitrile-water was pumped at a flow rate of 0.3 ml/min in gradient *** 2.6 m XB-C18 column(502.1 mm,100Å;Phenomenex,USA)were used for LC *** column temperature was 30℃ and the injection volume was 5 *** electrospray ionization(ESI)and multiple reaction monitoring(MRM)were applied at the transitions of m/z 457/279(azilsartan)and m/z 285/193(diazepam),*** developed method was identified a good linearity over a concentration range of 2.5e5000 ng/*** lower limit of quantitation(LLOQ)was 2.5 ng/*** intraday and inter-day precision(relative standard deviation,RSD%)were less than 10%and accuracy(relative error,RE%)was less than 5%at three quality control *** extraction recovery of azilsartan at three quality control levels were 82.41±0.68%,98.66±11.00%,102.43±0.82%.And the recovery for IS(100 ng/ml)was 91.75±0.54%.A validated UPLCeMS/MS method was firstly developed for the quantification of azilsartan in dog plasma and it was applied to the pharmacokinetics study.

关键词： Azilsartan UPLCeMS/MS Pharmacokinetics study

Serum gastrin-17 concentration for prediction of upper gastrointestinal tract bleeding risk among peptic ulcer patients

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World Journal of Clinical Cases 2021年第35期9卷 10948-10955页

作者： Jun-Xian Wang Yu-Ping Cao Peng Su Wei He Xiao-Ping Li Ya-meng zhu Department of Gastroenterology The Second People’s Hospital of Anhui ProvinceHefei 230011Anhui ProvinceChina

BACKGROUND Serum gastrin-17(G-17),pepsinogen I(PGI),and pepsinogen II(PGII)concentrations regulate gastric acid secretion,and hypersecretion of gastric acid increases the risks of peptic ulcer and upper gastrointestinal *** associations suggest that serum G-17,PGI,and(or)PGII may predict gastrointestinal bleeding risk among peptic ulcer *** To evaluate the efficacies of serum G-17,PGI,PGII,and PGI/PGII ratio(PGR)for predicting upper gastrointestinal bleeding among peptic ulcer *** A total of 199 patients diagnosed with peptic ulcer confirmed by gastroscopy and positivity for Helicobacter pylori by the 14C-urea breath test were recruited,including 107 patients with simple peptic ulcer and 92 cases complicated by upper gastrointestinal *** PGI,PGII,G-17,and PGR were measured by immune methods and compared between bleeding and non-bleeding groups by univariate *** specificity and sensitivity of PGs and G-17 for evaluating upper gastrointestinal bleeding risk were then assessed by constructing receiver operating characteristic(ROC)*** Serum G-17 was significantly higher among peptic ulcer patients with upper gastrointestinal bleeding compared to simple peptic ulcer patients(25.34±14.29 vs 8.84±8.03 pmol/L,t=9.822,P0.05).The risk of bleeding was significantly higher among peptic ulcer patients with elevated serum G-17(>15 pmol/L)compared to patients with normal or low serum G-17(73.2%vs 27.4%,χ2=40.72,P<0.01).The area under the ROC curve for serum G-17 was 0.866±0.024,and a cut-off of 9.86 pmol/L yielded 90.2%sensitivity and 68.2%specificity for distinguishing peptic ulcer with and without upper gastrointestinal *** Serum G-17 is significantly upregulated in peptic ulcer patients and higher levels are predictive of upper gastrointestinal ***,serum PGI,PGII,and PGR

关键词： Peptic ulcer Upper gastrointestinal bleeding Gastrin Pepsinogen Receiver operating characteristic curve