Inhibitive effect of TAK-242 on Tenon’s capsule fibroblasts proliferation in rat eyes

作     者：Liang Liang Meng-Nan Zhu Bao-Ji Chen Zheng Wang Li-Ye He Rang Zhang 

作者机构：Department of OphthalmologyThe First College of Clinical Medical ScienceChina Three Gorges UniversityYichang 443003Hubei ProvinceChina Department of OphthalmologyYichang Central People’s HospitalYichang 443003Hubei ProvinceChina Department of OphthalmologyXianning Central HospitalXianning 437100Hubei ProvinceChina 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2019年第12卷第11期

页      面：1699-1707页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by National Natural Science Foundation Program of China (No.81770920) Hubei Health and Family Planning Commission Youth Talent Project (No. WJ2017Q037) 

主　　题：Tenon's capsule fibroblasts fibrosis TAK-242 rat 

摘      要：AIM: To study the inhibition effect of TAK-242 on the proliferation of rat eye Tenon s capsule fibroblasts via the toll-like receptor 4(TLR4) signaling ***: SD rat Tenon s capsule fibroblasts were extracted and cultured, then the cells were divided into normal control group, lipopolysaccharide(LPS) group(10 g/m L LPS) and TAK-242 group(1 μmol/L TAK-242, and 10 μg/m L LPS after 30 min). The expressions of TLR4, transforming growth factor-β1(TGF-β1) and interleukin-6(IL-6) in each group were detected by Western blot and reverse transcriptase-polymerase chain reaction(RT-PCR). Cell proliferation was detected by cell counting kit-8(CCK-8).RESULTS: Double immunofluorescent labeling in the extracted cells showed negative keratin staining and positive vimentin staining. Western blot showed that the LPS group had the highest expression of TLR4 and TGF-β1(P0.05). RT-PCR showed that the IL-6 m RNA expression in LPS group was the highest in the three groups(P0.01). CONCLUSION: TAK-242 inhibits the proliferation of LPSinduced Tenon s capsule fibroblasts and the release of inflammatory factors by regulating the TLR4 signalingpathway, providing a new idea for reducing the scarring of the filter passage after glaucoma filtration surgery.