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Insect Science 2023年第1期30卷 208-220页

作者： Chao Zhang meng-sha Mao Xiang-Dong Liu Department of Entomology Nanjing Agricultural UniversityNanjingChina

Wing dimorphism is a fascinating feature of the ability of insects to adapt to *** brown planthopper(BPH)Nilaparvata lugens,a serious pest of rice,can switch between the long-and short-winged *** has been known that environmental factors can affect the wing morph of ***,it is still unclear whether the effect of environment is dependent on BPH genetic backgrounds or *** the present study,we established the pure-bred lineages of short-and long-winged BPHs via multigenerational selection,and we found that survival and fecundity were similar between these 2 *** morphs of the pure-bred lineages were almost fully dependent on genetics,but independent of the environmental factors,nymphal density and rice plant stage,2 key factors affecting BPH wing *** the unselected BPH population,short-and longwinged morphs were produced depending on those 2 environmental factors,indicating the contribution of environment to wing *** the wing-selected lineages,4 developmental regulated genes of wing,NllnRI,NllnR2,NIAkt,and NIFoxo were expressed stably in the short-winged adults,but almost silenced in the long-winged ***,all these genes were expressed normally with a similar level in both the short-and long-winged adults in an unselected population except *** pure-bred lineages of long-and short-winged morphs exhibited different expression patterns of wing development-regulated genes,suggesting the genetic determination of wing *** of environmental factors on wing morphs occurred only in the genetic mix population.

关键词： environmental effect gene expression genetic determination Nilaparvata lugens pure-bred lineage wing dimorphism

Research progress on microstructure evolution of semi-solid aluminum alloys in ultrasonic field and their rheocasting

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China Foundry 2014年第4期11卷 258-267页

作者： Wu Shusen Lin Chong Lü Shulin sha meng State Key Laboratory of Materials Processing and Die & Mould Technology Huazhong University of Science and Technology

The effects of ultrasonic vibration(UV)treatment on microstructure of semi-solid aluminum alloys and the application of UV in rheocasting process are *** semi-solid slurry can be produced by high-intensity UV process for aluminum *** microstructures of Al-Si,Al-Mg and Al-Cu alloys produced by rheocasting assisted with UV are compact and with fine *** mechanical properties of the UV treated alloys are increased by about 20%-30%.Grain refinement of the alloys is generally considered because of cavitation and acoustic streaming caused by *** from these mechanisms,a hypothesis of the fuse of dendrite root caused by capillary infiltration in the ultrasonic field,as well as a mechanism of crystallites falling off from the mould-wall and crystal multiplication by mechanical vibration effect in indirect ultrasonic vibration are proposed to explain the microstructure evolution of the alloys.

关键词： ultrasonic vibration aluminum alloy microstructure rheocasting

Protein trap-engineered metal-organic frameworks for advanced enzyme encapsulation and mimicking

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Nano Research 2023年第2期16卷 3364-3371页

作者： Weiqing Xu Yu Wu Lei Jiao meng sha Xiaoli Cai Yating Wen Yifeng Chen Wenling Gu Chengzhou Zhu Key Laboratory of Pesticide and Chemical Biology of Ministry of Education International Joint Research Center for Intelligent Biosensing Technology and HealthCollege of Chemistry Central China Normal UniversityWuhan 430079China

Immobilizing enzymes within metal-organic frameworks(MOFs)enables enzymes to against extreme ***,these MOF shells are just like armors,protective but heavy,which shield the enzymes from threats while locking them in the *** exploitation of immobilization strategy and intrinsic property of MOFs themselves is of great ***,we proposed a functional protein trap strategy for efficient enzyme *** ferrocenedicarboxylic acid(Fc)was used to induce the formation of defect-rich Co-based MOFs(CoBDC-Fc).As result,the engineered protein trap can not only improve the enzyme loading but also accelerate catalytic ***,the atomically dispersed Fc sites serve as cocatalysts/cofactors and even change the conformation of enzymes in the construed ***,the obtained CoBDC-Fc/enzyme exhibits excellent recyclability and tolerance to inhospitable *** by these,the CoBDC-Fc/enzyme/antigen composites were further prepared for cascade enzyme-linked immunosorbent assay of prostate-specific antigen with satisfactory sensitivity.

关键词： enzyme immobilization metal-organic frameworks biocatalysis nanozymes immunoassay

SSR fingerprinting of 203 sweetpotato (Ipomoea batatas (L.) Lam.) varieties

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Journal of Integrative Agriculture 2018年第1期17卷 86-93页

作者： meng Yu-sha ZHAO Ning LI Hui ZHAI Hong HE shao-zhen LIU Qing-chang Key Laboratory of Sweetpotate Biology and Biotechnolog Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization of Ministry of Education China Agricultural University Beijing 100193 P.R. China

Simple sequence repeat （SSR） markers have been shown to be a powerful tool for varieties identification in plants. How- ever, SSR fingerprinting of sweetpotato varieties has been a little reported. In this study, a total of 1 294 SSIR primer pairs, including 1 215 genomic-SSR and 79 expressed sequence tag （EST）-SSR primer pairs, were screened with sweetpotato varieties Zhengshu 20 and Luoxushu 8 and their 2 F1 individuals randomly sampled, and 273 and 38 of them generated polymorphic bands, respectively. Four genomic-SSR and 3 EST-SSR primer pairs, which showed good polymorphism, were selected to amplify 203 sweetpotato varieties and gave a total of 172 bands, 85 （49.42%） of which were polymorphic. All of the 203 sweetpotato varieties showed unique fingerprint patterns, indicating the utility of SSR markers in variety iden- tification of this crop. Polymorphism information content （PIC） ranged from 0.5824 to 0.9322 with an average of 0.8176. SSR-based genetic distances varied from 0.0118 to 0.6353 with an average of 0.3100 among these varieties. Thus, these sweetpotato varieties exhibited high levels of genetic similarity and had distinct fingerprint profiles. The SSR fingerprints of the 203 sweetpotato varieties have been successfully constructed. The highly polymorphic SSR primer pairs developed in this study have the potential to be used as core primer pairs for variety identification, genetic diversity assessment and linkage map construction in sweetpotato and other plants.

关键词： EST-SSR fingerprinting genetic distance genomic-SSR sweetpotato

Novel discovery of PDPN-positive CAFs contributing to tumor-associated lymphangiogenesis through mesenchymal to lymphatic endothelial transition in intrahepatic cholangiocarcinoma

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Genes & Diseases 2023年第6期10卷 2226-2228页

作者： meng sha Chuan Shen Seogsong Jeong Ning Xu Chen Chen Hua-lian Hang Ying Tong Jie Cao Department of Liver Surgery Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Department of Biomedical Informatics CHA University School of MedicineCHA UniversitySeongnam 13488Republic of Korea Institute of Basic Medical Sciences School of MedicineCHA UniversitySeongnam 13488Republic of Korea Institute for Biomedical Informatics School of MedicineCHA UniversitySeongnam 13488Republic of Korea

Tumor-associated lymphangiogenesis is thought to be an importantmetastaticstepin intrahepaticccholangiocarcinoma(ICC),while the origin of tumor-associated lymphatic vessels within tumors is little *** fibroblasts(CAFs)enhance tumor progression through many aspects.2 CAFs have been discovered exhibiting cellular plasticity of phenotypic transition-such as epithelial-mesenchymal transition(EMT)which promotes increased migratory and invasive capabilities of cells.3However,mesenchymal-to-endothelial,(MEndT)transition is yet to be identified in tumor entities.

关键词： CAFs lymphatic mesenchymal

Nitrogen and boron co-doped graphene nanoribbons as peroxidase-mimicking nanozymes for enhanced biosensing

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Chinese Chemical Letters 2022年第1期33卷 344-348页

作者： Siyu Luo meng sha Fei Tian Xialian Li Lijie Fu Yingqiu Gu Lu-Lu Qu Guo-Hai Yang Chengzhou Zhu School of Chemistry&Materials Science Jiangsu Normal UniversityXuzhou 221116China Key Laboratory of Pesticide and Chemical Biology of Ministry of Education International Joint Research Center for Intelligent Biosensing Technology and HealthCollege of ChemistryCentral China Normal UniversityWuhan 430079China

The rational design of nanozymes with superior activities is essential for improving bioassay ***,nitrogen and boron co-doped graphene nanoribbons(NB-GNRs)are prepared by a hydrothermal method using urea as the nitrogen source and boric acid as the boron source,*** introduction of co-doped and edge structures provides high defects and active *** resultant NB-GNRs nanozymes show superior peroxidase-like activities to nitrogen-doped and boron-doped counterparts due to the synergistic *** taking advantage of their peroxidase-like activities,NB-GNRs are used for the first time to develop enzyme-linked immunosorbent assay for the detection of *** biosensors exhibit a high performance with a linear range from 0.001 ng/mL to 1000 ng/mL and a detection limit of 0.3 pg/*** to their low cost and high stability,the proposed nanomaterials show great promise in biocatalysis,immunoassay development and environmental monitoring.

关键词： Graphene nanoribbons Heteroatom doping Nanozymes Biosensing Interleukin-6

Resistance Evaluation of Soybean Germplasm from Huanghuai Region to Phytophthora Root Rot

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Agricultural Sciences in China 2011年第2期10卷 246-251页

作者： TANG Qing-hua CUI Lin-kai LI De-long DAI Ting-ting YIN Wei-xiao DONG sha-meng XING Han ZHENG Xiao-bo WANG Yuan-chao Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects Ministry of Agriculture/Nanjing Agricultural UniversityNanjing 210095P.R.China National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095P.R.China

The aim of the study was to establish a set of differential strains and to identify soybean resistant genes to Phytophthora root rot and then to apply those strains for analysis of the resistant genes Rps1a,Rps1c,and Rps1k that soybean cultivars or lines may *** formula of 125 Phytophthora sojae isolates were determined using the hypocotyls inoculation technique,the strains,which includ 6 isolates with different virulence formulas,were applied to identify the resistance of 55 soybean cultivars or lines and resistant genes were analyzed using the gene postulating *** reaction types occurred in 55 cultivars or lines and results of gene postulation indicated that 2 cultivars or lines probably carried gene Rps1c and no cultivar may carry genes Rps1a or Rps1k.A few of soybean cultivars or lines from Huanghuai Region carry Rps genes Rps1a,Rps1c and Rps1k and tend to infect by ***,so resistant cultivars or lines need to be bred and popularized actively.

关键词： Phytophthora sojae differential strains soybean Phytophthora root rot resistance gene

CISG优先适用法律模式探析——兼评我国国际商事条约适用立法及司法实践

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东北大学学报（社会科学版） 2020年第4期22卷 97-103页

作者：杨梦莎天津大学法学院天津300072

国际商事条约的统一法宗旨决定了其具有优先于国内法适用的法律规则。以《联合国国际货物销售合同公约》为典型,其优先适用规则具有明确的国际法依据。而国内法层面如何处理国际商事条约与国内法之间的法律冲突,直接影响了法官优先适用... 详细信息

国际商事条约的统一法宗旨决定了其具有优先于国内法适用的法律规则。以《联合国国际货物销售合同公约》为典型,其优先适用规则具有明确的国际法依据。而国内法层面如何处理国际商事条约与国内法之间的法律冲突,直接影响了法官优先适用国际商事条约的效果和程度。该公约主要缔约国从本国已有宪法、立法、司法制度及法律原则出发,通过不同的法律路径实现了该公约的优先适用。目前我国司法实践仍存在适用《联合国国际货物销售合同公约》的分散和混乱状态,亟需通过加强立法供给和司法指导,明晰国际商事条约与国内法的法律适用关系。

关键词：《联合国国际货物销售合同公约》统一法优先适用法律模式

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客运专线常用跨度桥梁技术特点

桥梁建设 2007年第A01期37卷 1-3页

作者：孟莎中铁大桥局集团有限公司湖北武汉430050

客运专线的建设,带动了铁路桥梁建设整体技术水平的发展,也在一定程度上反映了高速条件下铁路桥梁的发展趋势。为了今后更加科学合理地开展高速铁路建设,有必要在建设过程中总结经验、系统测试,形成高速条件下的桥梁建设的一套较完善的... 详细信息

客运专线的建设,带动了铁路桥梁建设整体技术水平的发展,也在一定程度上反映了高速条件下铁路桥梁的发展趋势。为了今后更加科学合理地开展高速铁路建设,有必要在建设过程中总结经验、系统测试,形成高速条件下的桥梁建设的一套较完善的理论和技术。介绍客运专线常用跨度桥梁的设计、施工特点。

关键词：客运专线桥梁技术特点

An Analysis of the Changes of Gulliver's Character in Gulliver's Travels

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海外英语 2016年第21期 193-194页

作者：吴蒙莎 The School of Foreign Languages Ningbo Dahongying University

Most of the Domestic and foreign studies of Gulliver's Travels focus on the irony of the novel, there are few studies on the character of Gulliver. This paper will study the changes of Gulliver's character through the analysis of his travels in the four different countries, in order to contribute to a better understanding of Gulliver.

关键词： Gulliver’s Travels Gulliver Character Travel Changes