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ALK,the Key Gene for Gelatinization Temperature,is a Modifier Gene for Gel Consistency in Rice

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Journal of Integrative Plant Biology 2011年第9期53卷 756-765页

作者： Zhenyu Gao Dali Zeng Fangmin Cheng Zhixi Tian Longbiao Guo yan Su meixian yan Hua Jiang Guojun Dong Yuchen Huang Bin Han Jiayang Li Qian Qian State Key Laboratory of Rice Biology China National Rice Research InstituteChinese Academy of Agricultural ScienceHangzhou 310006China State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China National Center for Gene Research Chinese Academy of SciencesShanghai 200233China Department of Agronomy Huajia Chi CampusZhejiang UniversityHangzhou 310029China

Gelatinization temperature（GT） is an important parameter in evaluating the cooking and eating quality of ***,the phenotype,biochemistry and inheritance of GT have been widely studied in recent *** map-based cloning revealed that GT was controlled by ALK gene,which encodes a putative soluble starch synthase *** vector and RNAi vector were constructed and transformed into Nipponbare mediated by *** and molecular analyses of transgenic lines provided direct evidence for ALK as a key gene for ***,amylose content,gel consistency and pasting properties were also affected in transgenic *** of four nonsynonymous single nucleotide polymorphisms in coding sequence of ALK were identified as essential for *** on the single nucleotide polymorphisms（SNPs）,two new sets of SNP markers combined with one cleaved amplified polymorphic sequence marker were developed for application in rice quality breeding.

关键词： ALK gelatinization temperature gel consistency rice.

OsGRAS19 May Be a Novel Component Involved in the Brassinosteroid Signaling Pathway in Rice

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Molecular Plant 2013年第3期6卷 988-991页

作者： LishengChen Guosheng Xiong Xia Cui meixian yan Ting Xu Qian Qian Yongbiao Xue Jiayang Li Yonghong Wang State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Present address: Molecular Cellular and Developmental Biology University of Michigan Ann Arbor MI 48105 USA State Key Laboratory of Rice Biology China National Rice Research Institute Chinese Academy of Agricultural Sciences Hangzhou 310006 China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China

Dear Editor, The brassinosteroid （BR） signaling pathway has been well elucidated in Arabidopsis. The identification of BR-signaling components in rice suggested that the primary pathway is conserved in both dicot a... 详细信息

关键词： OsGRAS19 Component Involved

Identification and characterization of Mini1, a gene regulating rice shoot development

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Journal of Integrative Plant Biology 2015年第2期57卷 151-161页

作者： Yunxia Fang Jiang Hu Jie Xu Haiping Yu Zhenyuan Shi Guosheng Xiong Li Zhu Dali Zeng Guangheng Zhang Zhenyu Gao Guojun Dong meixian yan Longbiao Guo Yonghong Wang Qian Qian State Key Laboratory of Rice Biology China National Rice Research Institute Chinese Academy of Agricultural Sciences College of Agriculture and Biotechnology Zhejiang University Key Laboratory of Crop Physiology Ecology and Genetic Breeding Ministry of Education Jiangxi Agricultural University State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental Biology The Chinese Academy of Sciences

The aerial parts of higher plants are generated from the shoot apical meristem （SAM）. In this study, we isolated a small rice （Oryza sativa L.） mutant that showed premature termination of shoot development and was named mini rice 1 （mini1）. The mutant was first isolated from a japonica cultivar Zhonghua11 （ZH11） subjected to ethyl methanesulfonate （EMS） treatment. With bulked segregant analysis （BSA） and mapbased cloning method, Mini1 gene was finally fine-mapped to an interval of 48.6 kb on chromosome 9. Sequence analyses revealed a single base substitution from G to A was found in the region, which resulted in an amino acid change from Gly to Asp. The candidate gene Os09go363900 was predicted to encode a putative adhesion of calyx edges protein ACE （putative HOTHEAD precursor） and genetic complementation experiment confirmed the identity of Minil. Os09go36：3900 contains glucose-methanol-choline （GMC） oxidoreductase and NAD（P）-binding Rossmann-like domain, and exhibits high similarity to Arabidopsis HOTHEAD （HTH）. Expression analysis indicated Minil was highly expressed in young shoots but lowly in roots and the expression level of most genes involved in auxin biosynthesis and signal transduction were reduced in mutant. We conclude that Mini1 plays an important role in maintaining SAM activity and promoting shoot development in rice.

关键词： HOTHEAD mini rice shoot development

Some factors involved in microprojectile-mediated indica rice transformation

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Rice Science 1997年第4期 3-4页

作者： XUE Rui QIU Xiaohui yan meixian HUANG Danian CNRRI,Hangzhou 310006 CAO Shouyun TIAN Wenzhong Institute of Genetics,Academia Sinica,Beijing 100101,China

Immature embryos and calli derived from im-mature or mature embryos of the 16 indica ricevarieties have been transformed by using parti-cle *** improve growth state ofsubcultured calli,we set up a variety of pa-rameters to optimize the important factors in-volved in Biolistic system,and added varioushormones,mannitol,and MES to CC or NB

关键词： CC NB rate Some factors involved in microprojectile-mediated indica rice transformation than

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减温减压器在流化床干燥器的实际应用

中国仪器仪表 2022年第8期 62-65页

作者：孙正光严梅仙邱悦吉林石化公司电仪中心吉林吉林132021 吉林石化建修中心吉林吉林132021 广东石化公司化工二部广东揭阳522000

本文简要论述了流化床干燥器工艺流程,介绍了减温减压器结构及组成,并对减温减压器日常维护及故障处理进行描述,并详细论述了减温减压器的控制系统组成和PID控制经验调试。

关键词：流化床减温减压器控制系统 PID控制

右美托咪定联合甲磺酸罗哌卡因肋间神经阻滞改善多发性肋骨骨折患者疼痛、睡眠的研究

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中国医师进修杂志 2019年第6期42卷 518-521页

作者：严美仙赵伟胡惠森浙江大学医学院附属第二医院兰溪分院麻醉科浙江兰溪321100

目的研究右美托咪定联合甲磺酸罗哌卡因行肋间神经旁注射进行神经阻滞对多发性肋骨骨折患者疼痛、睡眠的影响。方法选取2017年9月至2018年9月浙江大学医学院附属第二医院兰溪分院收治的178例多发性肋骨骨折患者,按随机数字表法分为对照... 详细信息

目的研究右美托咪定联合甲磺酸罗哌卡因行肋间神经旁注射进行神经阻滞对多发性肋骨骨折患者疼痛、睡眠的影响。方法选取2017年9月至2018年9月浙江大学医学院附属第二医院兰溪分院收治的178例多发性肋骨骨折患者,按随机数字表法分为对照组(甲磺酸罗哌卡因)88例和试验组(右美托咪定联合甲磺酸罗哌卡因)90例,入院第二天在超声引导下行肋间神经阻滞。观察、比较两组阻滞后各时间点的静息痛、咳嗽痛、夜间疼痛等评分,以及阻滞后3天睡眠情况等。结果在4~7根肋骨骨折患者中,16 h内试验组中静息疼痛评分均明显低于对照组[(1.3 ± 0.6)分比(1.7 ± 0.7)分、(2.1 ± 0.4)分比(2.2 ± 0.6)分、(2.2 ± 0.3)分比(2.3 ± 0.3)分];而在咳嗽疼痛评分中,试验组在24 h内疼痛评分明显优于对照组[(1.6 ± 0.8)分比(2.5 ± 0.9)分、(3.0 ± 0.7)分比(3.3 ± 0.8)分、(3.3 ± 0.7)分比(4.0 ± 0.9)分、(4.9 ± 1.0)分比(5.4 ± 1.0)分],差异均有统计学意义(P < 0.05)。在大于7根肋骨骨折患者中,在12 h之内,无论是静息疼痛评分还是咳嗽疼痛评分比较,试验组均明显优于对照组,差异有统计学意义(P < 0.05)。阻滞后第一天、第二天,试验组中失眠评分明显低于对照组[(2.5 ± 0.7)分比(4.2 ± 1.6)分、(2.8 ± 0.8)分比(4.5 ± 1.5)分],差异有统计学意义(χ2值分别为7.347、7.989,P < 0.05);两组均未发生不良反应。结论对于多发性肋骨骨折患者,应用右美托咪定联合甲磺酸罗哌卡因行肋间神经旁注射,能改善镇痛效果,延长镇痛时间,改善睡眠质量。

关键词：肋骨骨折肋间神经神经传导阻滞镇痛右美托咪定

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离心泵封液系统液位测量误差分析

中国仪器仪表 2022年第7期 90-92页

作者：张俊赫孙海英罗炎贻严梅仙吉林石化(揭阳)分公司广东揭阳522000 广东石化公司化工二部广东揭阳522000 吉林石化建修公司吉林吉林132021

本文介绍了离心泵双端密封工作原理,论述了离心泵密封液系统液位测量原理,分析了密封液系统测量误差,并提出改进意见。

关键词：双端面密封封液系统误差分析

两个水稻DH群体发芽期和幼苗前期耐碱性状QTL定位比较

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分子植物育种 2008年第3期6卷 439-450页

作者：程海涛姜华颜美仙董国军钱前郭龙彪沈阳农业大学农学院沈阳110161 水稻生物学国家重点实验室中国水稻研究所杭州310006

利用两个不同的加倍单倍体群体,在0.15%Na2CO3胁迫下,以发芽期和幼苗前期的发芽势(GE)等10个性状碱害相对值作为耐碱指标,进行耐碱性状QTL定位比较。碱害相对值相关分析表明,多个性状的碱害相对值存在显著或极显著相关。采用QTLMapper1.... 详细信息

利用两个不同的加倍单倍体群体,在0.15%Na2CO3胁迫下,以发芽期和幼苗前期的发芽势(GE)等10个性状碱害相对值作为耐碱指标,进行耐碱性状QTL定位比较。碱害相对值相关分析表明,多个性状的碱害相对值存在显著或极显著相关。采用QTLMapper1.6统计软件对碱害相对值进行QTL分析。DH-1群体定位到10个主效QTL和15个上位性QTL,DH-2群体定位到14个主效QTL和15个上位性QTL。两群体定位列线比对发现:1个控制相对根长的QTL,qRRL3-1,两群体定位在第3染色体对应的区域(CT339-G62和RM7-RM3280)。其他性状两群体没有定位在相同的区域,但存在多个重要的数量基因座位,如CT158-CT550、RM3755-RM418和RM1349-RM1061等区域。在这些相同基因座位上两群体都检测到控制不同性状的主效QTL或上位性QTL,耐碱性QTL可能存在多效性、连锁性,在不同遗传背景下加性效应和上位性效应可以相互转化。多个QTL不仅与水稻耐碱性有关,还与多种抗逆性有关。这些结果将有利于耐碱性分子机制的剖析和强耐碱性水稻品种的选育。

关键词：水稻 DH群体发芽期和幼苗前期耐盐碱性状 QTL

Isolation and functional analysis of CS-2b gene for chlorate sensitivity in rice

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Isolation and functional analysis of CS-2b gene for chlorate...