Identification and characterization of Mini1, a gene regulating rice shoot development

作     者：Yunxia Fang Jiang Hu Jie Xu Haiping Yu Zhenyuan Shi Guosheng Xiong Li Zhu Dali Zeng Guangheng Zhang Zhenyu Gao Guojun Dong Meixian Yan Longbiao Guo Yonghong Wang Qian Qian 

作者机构：State Key Laboratory of Rice Biology China National Rice Research Institute Chinese Academy of Agricultural Sciences College of Agriculture and Biotechnology Zhejiang University Key Laboratory of Crop Physiology Ecology and Genetic Breeding Ministry of Education Jiangxi Agricultural University State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental Biology The Chinese Academy of Sciences 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2015年第57卷第2期

页      面：151-161页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by grants from the National Natural Science Foundation of China (No. 31201194) 

主　　题：HOTHEAD mini rice shoot development 

摘      要：The aerial parts of higher plants are generated from the shoot apical meristem （SAM）. In this study, we isolated a small rice （Oryza sativa L.） mutant that showed premature termination of shoot development and was named mini rice 1 （mini1）. The mutant was first isolated from a japonica cultivar Zhonghua11 （ZH11） subjected to ethyl methanesulfonate （EMS） treatment. With bulked segregant analysis （BSA） and mapbased cloning method, Mini1 gene was finally fine-mapped to an interval of 48.6 kb on chromosome 9. Sequence analyses revealed a single base substitution from G to A was found in the region, which resulted in an amino acid change from Gly to Asp. The candidate gene Os09go363900 was predicted to encode a putative adhesion of calyx edges protein ACE （putative HOTHEAD precursor） and genetic complementation experiment confirmed the identity of Minil. Os09go36：3900 contains glucose-methanol-choline （GMC） oxidoreductase and NAD（P）-binding Rossmann-like domain, and exhibits high similarity to Arabidopsis HOTHEAD （HTH）. Expression analysis indicated Minil was highly expressed in young shoots but lowly in roots and the expression level of most genes involved in auxin biosynthesis and signal transduction were reduced in mutant. We conclude that Mini1 plays an important role in maintaining SAM activity and promoting shoot development in rice.