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Journal of Integrative Agriculture 2018年第11期17卷 2478-2491页

作者： Sumaira Farrakh meinan wang Xianming Chen Department of Plant Pathology Washington State University Department of Biosciences COMSATS Institute of Information Technology USDA-ARS Wheat Health Genetics and Quality Research Unit

Interactions of the stripe rust pathogen （Puccinia striiformis f. sp responses. Among various genes involved in the plant-pathogen related （PR） protein genes determine different defense responses tritici） with wheat plants activate a w^ae range OT nost nteractions, the expressions of particular pathogenesis-Different types of resistance have been recognized and utilized for developing wheat cultivars for resistance to stripe rust. All-stage resistance can be detected in seedling stage and remains at high levels throughout the plant growth stages. This type of resistance is race-specific and not durable. In contrast, plants with only high-temperature adult-plant （HTAP） resistance are susceptible in seedling stage, but become resistant when plants grow older and the weather becomes warmer. HTAP resistance controlled by a single gene is partial, but usually non-race specific and durable. The objective of this study was to analyze the expression of PR protein genes involved in different types of wheat resistance to stripe rust. The expression levels of 8 PR protein genes （PR1, PRI.2, PR2, PR3, PR4, PR5, PR9 and PRIO） were quantitatively evaluated at 0, 1, 2, 7 and 14 days after inoculation in single resistance gene lines of wheat with all-stage resistance genes YrTrl, Yr76, YrSP and YrExp2 and lines carrying HTAP resistance genes Yr52, Yr59, Yr62 and Yr7B. Races PSTv-4 and PSTv-37 for compatible and incompatible interactions were used in evaluation of PR protein gene expression in wheat lines carrying all-stage resistance genes in the seedling- stage experiment while PSTv-37 was used in the HTAP experiment. Analysis of quantitative real-time polymerase chain reaction （qRT-PCR） revealed that all of the PR protein genes were involved in the different types of resistance controlled by different Yr genes. However, these genes were upregulated at different time points and at different levels during the infection process among the wheat lines with different Yr genes for eith

关键词： wheat stripe rust plant resistance pathogen-related protein qRT-PCR

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Effect of Alkaline Electrolyzed Water on Performance Improvement of Green Concrete with High Volume of Mineral Admixtures

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Journal of Renewable Materials 2021年第11期9卷 2051-2065页

作者： Guibin Liu meinan wang Qi Yu Qiuyi Li Liang wang Center for Technological Development Qingdao Qingjian New Material Group Co.Ltd.Qingdao266108China College of Civil Engineering&Architecture Qingdao Agricultural UniversityQingdao266109China

The strength and durability of concrete will be significantly reduced at high volume of mineral admixture,and the poor early strength of concrete also still needs to be *** this investigation,a highly active alkaline electrolyzed waters was used as mixing water to improve the early strength and enhance the durability of green concrete with high volume mineral admixture,the influences of alkaline electrolyzed water(AEW)on hydration activity of mineral admixture and durability of concrete were *** results showed that compared with natural tap water,AEW can accelerate early hydration process of cement in concrete and produce comparatively more hydrated products,leading to a 13.6%higher compressive strength than that of ordinary concrete at early age,but the improvement effect of AEW concrete was relatively reduced at long-term ***,the activity of mineral admixtures could be stimulated by AEW to some extent,the strength and durability performance of AEW concrete after double doping 25%slag and 25%fly ash can still reach the level of ordinary cement concrete without mineral *** SEM micromorphology of 7 d hydrated natural tap water cement paste was observed to be flaky and tabular,but the AEW cement pastes present obvious cluster and granulation *** SEM microstructure of AEW concrete with mineral admixtures is more developed and denser than ordinary tap water concrete with mineral ***,the AEW probably could realize the effective utilization of about 50%mineral admixture amount of concrete without strength loss,the cement production cost and associated CO_(2) emission reduced,which has a good economic and environmental benefit.

关键词： Alkaline electrolyzed water durability improvement green concrete mineral admixture micromorphology

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Gene expression profiling of Puccinia striiformis *** during development reveals a highly dynamic transcriptome

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Journal of Genetics and Genomics 2011年第8期38卷 357-371页

作者： Xueling Huang Xianming Chen Tristan Coram meinan wang Zhensheng Kang State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F University Yangling Shaanxi 712100 PR China Department of Plant Pathology Washington State University Pullman WA 99164-6430 USA United States Department of Agriculture Agricultural Research Service Wheat Genetics Quality Physiology and Disease Research Unit Pullman WA 99164-6430 USA

Puccinia striiformis f. sp. tritici （PsO causes stripe rust, one of the most important diseases of wheat worldwide, cDNA libraries had been constructed from urediniospores, germinated urediniospores and haustoria. However, little is known about the expression patterns of the genes related to the infection process and sporulation of the pathogen. In this study, a custom oligonucleotide microarray was constructed using sequences of 442 gene transcripts selected from Pst cDNA libraries. The expression patterns of the genes were determined by hybridizing the microarray with cDNA from Pst in vitro and Pst-infected wheat leaves. The time course study identified 55 transcripts that were differentially expressed during the infection process in a compatible interaction. They were identified to have functions related to the following biological processes, including carbohydrate and lipid metabolism, energy, cell signaling, protein synthesis, cell structure and division. In an incompatible interaction, 17 transcripts of the pathogen were differentially expressed in resistant wheat leaves inoculated with an avirulent Pst race, ten of which had similar expression patterns to those in the compatible interaction. Several candidates for pathogenicity and virulence/avirulence related genes were also identified. The results of quantitative real-time PCR validated the expression patterns of some selected genes. The study demonstrates that the custom oligonucleotide microarray technology is useful to determine the expression patterns of the pathogen genes involved in different types of the host--pathogen interactions and stages of development.

关键词： Puccinia striifonnis Stripe rust Yellow rust Expression profiling Microarray Wheat

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Establishment of Unstable Flow Model and Well Testing Analysis for Viscoelastic Polymer Flooding

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World Journal of Engineering and Technology 2023年第2期11卷 273-280页

作者： Zheng Lv meinan wang Bohai Oilfield Research Institute of CNOOC Ltd. Tianjin Branch Tianjin China

At present, the polymer solution is usually assumed to be Newtonian fluid or pseudoplastic fluid, and its elasticity is not considered on the study of polymer flooding well testing model. A large number of experiments have shown that polymer solutions have viscoelasticity, and disregarding the elasticity will cause certain errors in the analysis of polymer solution seepage law. Based on the percolation theory, this paper describes the polymer flooding mechanism from the two aspects of viscous effect and elastic effect, the mathematical model of oil water two-phase three components unsteady flow in viscoelastic polymer flooding was established, and solved by finite difference method, and the well-test curve was drawn to analyze the rule of well test curve in polymer flooding. The results show that, the degree of upward warping in the radial flow section of the pressure recovery curve when considering polymer elasticity is greater than the curve which not considering polymer elasticity. The relaxation time, power-law index, polymer injection concentration mainly affect the radial flow stage of the well testing curve. The relaxation time, power-law index, polymer injection concentration and other polymer flooding parameters mainly affect the radial flow stage of the well testing curve. The larger the polymer flooding parameters, the greater the degree of upwarping of the radial flow derivative curve. This model has important reference significance for well-testing research in polymer flooding oilfields.

关键词： Polymer Flooding Viscoelasticity Well Testing Mathematical Model Seepage Law

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Variation in Telial Formation of <i>Puccinia striiformis</i>in the United States

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American Journal of Plant Sciences 2019年第5期10卷 826-849页

作者： Anmin Wan meinan wang Xianming Chen Department of Plant Pathology Washington State University Pullman USA United States Department of Agriculture Agricultural Research Service Wheat Health Genetics and Quality Research Unit Pullman USA

Puccinia striiformis, the causal agent of stripe rust in wheat, barley, and various wild grasses, produces urediniospores and teliospores on these primary or auxiliary hosts. Telial formation, which stops producing infectious urediniospores, is affected by various host and environmental conditions. However, it is not clear if variation exists among different isolates in the United States. To determine the differences in the pathogen population, 1423 isolates collected in the United States from 2013 to 2016 were tested for telial formation in the seedling stage at a diurnal temperature profile of 4°C - 20°C. The percentages of telial formation varied greatly among the isolates. Of the 1423 isolates, 62.97% produced telia with the percentages of telial formation ranging from 1% to 95% under the test conditions. The formation of telia was significantly affected by the year and the month when the isolates were collected. The epidemiological regions or states, host plants (wheat, barley, and grasses), and races of the isolates did not significantly affect telial formation. However, significant effects on telial formation were observed by interactions between year and region, year and race, month and region and among year, month, and region, as well as between year and month. The results showed that telial formation is a complex trait under the genetic control of the pathogen isolates for adaptation to different environments. Further studies are needed to identify genes involved in the formation of telia and the relationship of telial formation to the survival, aggressiveness, fitness, and evolution of the pathogen.

关键词： Puccinia striiformis Stripe Rust Telial Formation Wheat

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Injection Scheme Optimization of Foam Profile Control on Block S103

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Engineering（科研） 2014年第1期6卷 7-11页

作者： Hongjun Yin Chuan Liu meinan wang Accumulation and Development of Unconventional Oil and Gas State Key Laboratory Cultivation Base Jointly-Constructed by Heilongjiang Province and the Ministry of Science and Technology Northeast Petroleum University Daqing China Key Laboratory of Ministry of Education PRC Northeast Petroleum University Daqing China

Facing problems with oil production decreasing quickly and water content increasing gradually in S103 well area, the Foam Profile Control was studied on the basis of its confirmed agent formula. The facies-controlled geological model of S103 well area was generated using random model based on Petrel software and using numerical simulation based on CMG software. Gas liquid alternating injection pattern was optimized as the optimal solution through the simulation optimization. Two months are optimized as the optimal solution through the simulation optimization. The cumulative oil production is 0.933 × 107 kg which is higher than the value of subsequent water flooding and the other three. Finally, it reaches the goals of slowing down the production decline and controlling the water rising.

关键词： Foam Profile Control Numerical Simulation Injection Pattern Injection Cycle

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Research on Optimization Design of Deep Profile Control Injection Scheme in Block S

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Open Journal of Applied Sciences 2023年第11期13卷 2047-2054页

作者： meinan wang Shaopeng wang Wei Zhang Xiaoqi Chen Junting Zhang Bohai Oilfield Research Institute of CNOOC Ltd.-Tianjin Branch Tianjin China

In view of the rapid decline rate of oil production and gradual increase of water cut in oil wells in Block S, based on the determination of the formula of foam profile control agent, the optimization study of foam profile control injection scheme was carried out. Petrel software is used to establish a facies controlled geological model based on stochastic modeling method in Block S, and CMG software is used for numerical simulation to design 7 sets of foam profile control prediction schemes. Dynamic data and numerical simulation methods are used to optimize foam injection mode and injection cycle. By simulating and calculating, the optimal injection method of alternating gas and liquid injection and the optimal plan with an injection period of 2 months were selected. Through the calculation results of various research plans, it can be seen that the water content of the optimal plan has decreased significantly, and the oil production rate has increased significantly, in order to slow down production decline, control water content rise, extend the economic recovery period of the oilfield, and achieve the goal of improving oil recovery.

关键词： Foam Profile Control Numerical Simulation Injection Method Injection Cycle

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Study on Optimization of Polymer Enhanced Foam Formulation System

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Engineering（科研） 2023年第11期15卷 742-748页

作者： meinan wang Yuejie wang Hongyou Zhang Xiaoyan Yang Xiaoming Chen Bohai Oilfield Research Institute of CNOOC Ltd.—Tianjin Branch Tang Gu Tianjin China

Taking the pilot test area of polymer enhanced foam flooding in Y oilfield as the prototype, a numerical core model was established, and the polymer enhanced foam formulation system was optimized by analyzing the resistance factor and the change rule of oil recovery of different formulation systems. Research shows that the higher the polymer concentration, the greater the resistance factor, and the more obvious the sealing effect formed in the formation. The concentration of surfactant has a greater impact on the resistance factor. With the increase of surfactant concentration, the resistance factor increases significantly, and the plugging effect of foam agent on core is significantly enhanced. With the increase of gas-liquid ratio, the resistance factor first increases and then decreases. When the gas-liquid ratio is 1:1, the resistance is the largest, and the foam agent has the strongest plugging effect on the core. The optimal formula system of polymer enhanced foam flooding in Y oilfield is: polymer concentration is 1200 mg/L, surfactant concentration is 0.25 wt%, gas-liquid ratio is 1:1.

关键词： Enhanced Foam Polymer Concentration Surfactant Concentration Gas-Liquid Ratio

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Research and Practice on the Profile Control Characteristics of Emulsions in Bohai Oil Field

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Journal of Geoscience and Environment Protection 2018年第8期6卷 52-59页

作者： Dongdong Yang meinan wang Feifei Miao Shaopeng wang Junting Zhang Bohai Oilfield Research Institute of CNOOC Ltd.-Tianjin Branch Tang Gu Tianjin China

In order to study the profile control characteristics of emulsions in porous medium, the heterogeneous experimental model was established, by which the experimental scheme was designed with different permeabilities, matching degree of particle size and concentration of emulsions. Based on experimental results of physical simulation, the concept of distributing rate and changing coefficient was introduced to quantitatively evaluate the profile control characteristics of emulsions. The results of the study show that, as the permeability ratio and emulsion concentration increase, the profile control characteristics of emulsions will enhance. The profile control characteristics of emulsions will be the best. The profile control scheme with emulsions was implemented in Bohai X oil field, The research results can be used for reference to design the profile control scheme in Bohai heterogeneous reservoir.

关键词： Emulsions Profile Control Characteristics Physical Simulation Heterogeneous Reservoir Practice

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Diagnostic Method and Adaptability Analysis of Multiple Water Breakthroughs in Horizontal Well in Combined Well Pattern

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Journal of Geoscience and Environment Protection 2023年第5期11卷 150-158页

作者： Zheng Lv meinan wang Guohao Zhang Rui Zhang Jianguo Liu Bohai Oilfield Research Institute of CNOOC Ltd.-Tianjin Branch Tianjin China

The combined well pattern has been widely used in reservoir development, with a common pattern being a horizontal well in the center for oil production and multiple vertical wells around for water injection. For this type of well pattern, determining the horizontal well is affected by which injection wells, especially when the injecting water breaks through, accurately determining the direction of water inflow will provide an important basis for targeted water well measures. Based on the production performance data of horizontal wells, the semi logarithmic relationship curves of water-oil ratio, derivative water-oil ratio, and cumulative production were used for the first time to determine the breakthrough problem of water injection in the surrounding water injection wells of horizontal wells based on their response characteristics. The adaptability of this method under different influencing factors was analyzed. Introducing the parameter of cumulative production not only preserves the variation trend of the derivative of water-oil ratio with time, but also facilitates the processing of actual production data.

关键词： Combined Well Pattern Multiple Water Breakthroughs in Horizontal Derivative of Water-Oil Ratio

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