Pathogenesis-related protein genes involved in race-specific allstage resistance and non-race specific high-temperature adultplant resistance to Puccinia striiformis f. sp. tritici in wheat

作     者：Sumaira Farrakh Meinan Wang Xianming Chen 

作者机构：Department of Plant Pathology Washington State University Department of Biosciences COMSATS Institute of Information Technology USDA-ARS Wheat Health Genetics and Quality Research Unit 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2018年第17卷第11期

页      面：2478-2491页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：supported by the U.S. Department of Agriculture, Agricultural Research Service (2090-22000018-00D) the Washington Grain Commission, USA (13C3061-5665) the Idaho Wheat Commission, USA (13C3061-5665 13C-3061-4232) The Fulbright fellowship 

主　　题：wheat stripe rust plant resistance pathogen-related protein qRT-PCR 

摘      要：Interactions of the stripe rust pathogen （Puccinia striiformis f. sp responses. Among various genes involved in the plant-pathogen related （PR） protein genes determine different defense responses tritici） with wheat plants activate a w^ae range OT nost nteractions, the expressions of particular pathogenesis-Different types of resistance have been recognized and utilized for developing wheat cultivars for resistance to stripe rust. All-stage resistance can be detected in seedling stage and remains at high levels throughout the plant growth stages. This type of resistance is race-specific and not durable. In contrast, plants with only high-temperature adult-plant （HTAP） resistance are susceptible in seedling stage, but become resistant when plants grow older and the weather becomes warmer. HTAP resistance controlled by a single gene is partial, but usually non-race specific and durable. The objective of this study was to analyze the expression of PR protein genes involved in different types of wheat resistance to stripe rust. The expression levels of 8 PR protein genes （PR1, PRI.2, PR2, PR3, PR4, PR5, PR9 and PRIO） were quantitatively evaluated at 0, 1, 2, 7 and 14 days after inoculation in single resistance gene lines of wheat with all-stage resistance genes YrTrl, Yr76, YrSP and YrExp2 and lines carrying HTAP resistance genes Yr52, Yr59, Yr62 and Yr7B. Races PSTv-4 and PSTv-37 for compatible and incompatible interactions were used in evaluation of PR protein gene expression in wheat lines carrying all-stage resistance genes in the seedling- stage experiment while PSTv-37 was used in the HTAP experiment. Analysis of quantitative real-time polymerase chain reaction （qRT-PCR） revealed that all of the PR protein genes were involved in the different types of resistance controlled by different Yr genes. However, these genes were upregulated at different time points and at different levels during the infection process among the wheat lines with different Yr genes for eith