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Food Science and Human Wellness 2024年第3期13卷 1645-1661页

作者： Xiuqing Yang Sizhu Li Xiaohang Li Chenxiao Zhang meijie liu Lizhong Guo Lin liu Hao Yu Shandong Provincial Key Laboratory of Applied Mycology School of Life SciencesQingdao Agricultural UniversityQingdao 266109China

White Hypsizygus marmoreus is a popular edible *** mycelium is easy to be contaminated by Penicillium,which leads to a decrease in its quality and *** could compete for limited space and nutrients through rapid growth and produce a variety of harmful gases,such as benzene,aldehydes,phenols,etc.,to inhibit the growth of *** mycelium.A series of changes occurred in *** proteome after contamination when detected by the label-free tandem mass spectrometry(MS/MS)*** proteins with up-regulated expression worked together to participate in some processes,such as the non-toxic transformation of harmful gases,glutathione metabolism,histone modification,nucleotide excision repair,clearing misfolded proteins,and synthesizing glutamine,which were mainly used in response to biological *** proteins with down-regulated expression are mainly related to the processes of ribosome function,protein processing,spliceosome,carbon metabolism,glycolysis,and *** reduction in the function of these proteins affected the production of the cell components,which might be an adjustment to adapt to growth *** study further enhanced the understanding of the biological stress response and the growth restriction adaptation mechanisms in edible *** also provided a theoretical basis for protein function exploration and edible mushroom food safety research.

关键词： Hypsizygus marmoreus Penicillium Proteomics Biological stress response Adaptation

左归丸含药血清对Hedgehog-GLi通路关键因子mRNA表达及骨吸收功能的影响

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中国骨质疏松杂志 2024年第6期30卷 807-812页

作者：廖泽绮史婧儒王雨荷赵宏艳张元月刘梅洁中国中医科学院医学实验中心北京100700

目的探讨左归丸含药血清对成骨-破骨细胞共培养体系中Hedgehog-GLi通路关键因子mRNA表达及骨吸收功能的影响。方法建立成骨细胞、破骨细胞、骨磨片共培养体系,实验分为7组:空白对照组、假手术组、OVX组、阳性对照组、左归丸组、激动剂... 详细信息

目的探讨左归丸含药血清对成骨-破骨细胞共培养体系中Hedgehog-GLi通路关键因子mRNA表达及骨吸收功能的影响。方法建立成骨细胞、破骨细胞、骨磨片共培养体系,实验分为7组:空白对照组、假手术组、OVX组、阳性对照组、左归丸组、激动剂组和激动剂+左归丸组。选用50只雌性SD大鼠,分别制备相对应组含药血清,实验各组分别加入相对应组大鼠血清。通过实时荧光定量PCR检测成骨细胞Ihh、Ptch、Smo、GLi1、OPG及RANKL的mRNA表达;并采用甲苯胺蓝染色方法检测破骨细胞骨吸收功能。结果与假手术组相比,OVX组Ihh、Ptch、Smo、GLi1mRNA表达显著上调,同时OPGmRNA表达水平显著降低,RANKL mRNA表达水平显著增加,RANKL/OPG比值明显增高。与OVX组相比,阳性对照组与左归丸组Ihh、Ptch、Smo、GLi1mRNA表达明显下调,同时OPG mRNA表达水平显著升高,RANKL mRNA表达水平显著降低,RANKL/OPG比值明显下降。OVX组加入激动剂Shh重组蛋白后,Hedgehog-GLi信号通路关键因子的mRNA表达较OVX组明显增加。与激动剂组比较,激动剂+左归丸组Ihh、Ptch、Smo、GLi1mRNA表达以及RANKL/OPG比值均明显下降。与假手术组相比,OVX组的骨吸收陷窝面积显著增加;与OVX组相比,阳性对照组和左归丸组的骨吸收陷窝面积显著下降。结论左归丸含药血清可一定程度上抑制Hedgehog-GLi通路关键因子Ihh、Ptch、Smo、GLi1的活性,进而抑制OVX所致的骨吸收增强。

关键词：左归丸骨质疏松症 Hedgehog-GLi信号通路成骨细胞破骨细胞共培养体系

“三全育人”理念在法学虚拟仿真实验教学中的渗透

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中阿科技论坛（中英文） 2024年第8期 142-146页

作者：李宏斌许赢予刘美洁喀什大学新疆喀什844000

高校是人才培养的主阵地,理应全面贯彻党的教育方针,落实立德树人根本任务,坚持德智体美劳全面发展,把全员、全程、全方位“三全育人”落到实处,办好人民满意教育,着力培养担当民族复兴大任的时代新人。“三全育人”体现了高等教育立德... 详细信息

高校是人才培养的主阵地,理应全面贯彻党的教育方针,落实立德树人根本任务,坚持德智体美劳全面发展,把全员、全程、全方位“三全育人”落到实处,办好人民满意教育,着力培养担当民族复兴大任的时代新人。“三全育人”体现了高等教育立德树人的内在要求,契合了新时代全面发展型人才培养的客观需要,激活了高校实现内涵式发展的资源要素。基于此,文章对法学虚拟仿真实验教学现状进行分析,进而深入探讨“三全育人”理念在法学虚拟仿真实验教学中的应用原则与策略,旨在实现法学实验教学的创新和发展,培养符合时代发展要求的优秀法律人才。

关键词： “三全育人”理念法律人才法学虚拟仿真实验教学

Thin paints for durable and scalable radiative cooling

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Journal of Energy Chemistry 2024年第3期90卷 176-182,I0006页

作者： Shanquan liu Fei Zhang Xingyu Chen Hongjie Yan Wei Chen meijie Chen School of Energy Science and Engineering Central South UniversityChangsha 410083HunanChina School of Intelligent Manufacturing Ecosystem Xi'an Jiaotong-Liverpool UniversitySuzhou 215400JiangsuChina

Passive daytime radiative cooling(PDRC) is environment-friendly without energy input by enhancing the coating's solar reflectance(R_(solar)) and thermal emittance(ε_(LWIR)) in the atmosphere's long-wave infrared transmission ***,high R_(solar) is usually achieved by increasing the coating's thickness,which not only increases materials' cost but also impairs heat ***,the desired high R_(solar) is vulnerable to dust pollution in the *** this work,a thin paint was designed by mixing hBN plates,PFOTS,and IPA. R_(solar)=0.963 and ε_(LWIR)=0.927 was achieved at a thickness of 150 μm due to the high backscattering ability of scatters.A high through-plane thermal conductivity(~1.82 W m^(-1) K^(-1)) also can be *** addition,the porous structure coupled with the binder PFOTS resulted in a contact angle of 154°,demonstrating excellent durability under dust *** experiments showed that the thin paint can obtain a 2.3℃ lower temperature for sub-ambient cooling than the reference PDRC coating in the ***,the above-ambient heat dissipation performance can be enhanced by spraying the thin paint on a 3D heat sink,which was 15.7℃ lower than the reference 1D structure,demonstrating excellent performance for durable and scalable PDRC applications.

关键词： Radiative cooling Heat dissipation Solar reflectance Thermal emittance Contact angle

急性缺氧致小鼠脑血管平滑肌功能改变的转录组学分析

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空军军医大学学报 2024年第2期45卷 198-205页

作者：常玮李亚娟臧克海李少华刘美杰刘峰舟薛军辉空军军医大学航空航天医学系航空航天临床医学中心陕西西安710032 空军军医大学航空航天医学系航空航天生理学教研室陕西西安710032 空军军医大学航空航天医学系飞行人员疗养与康复教研室陕西西安710032 空军军医大学西京医院空勤科陕西西安710032

目的探讨小鼠脑血管平滑肌细胞(CVSMCs)在低气压缺氧脑损伤中的作用及其可能的发生机制。方法将雄性C57BL/6J小鼠随机分为平原组(Sham组)和高原组(HA组),每组10只。采用HE染色、免疫组织化学法、免疫荧光法、Western blotting观察各组... 详细信息

目的探讨小鼠脑血管平滑肌细胞(CVSMCs)在低气压缺氧脑损伤中的作用及其可能的发生机制。方法将雄性C57BL/6J小鼠随机分为平原组(Sham组)和高原组(HA组),每组10只。采用HE染色、免疫组织化学法、免疫荧光法、Western blotting观察各组小鼠脑损伤以及脑血管平滑肌功能变化情况。分离培养另20只C57BL/6J小鼠的原代CVSMCs并构建细胞缺氧模型。采用RNA-seq技术对缺氧组(Hypoxia组)和常氧对照组(Control组)的细胞进行转录组测序,筛选出差异表达基因(DEGs)后进行GO富集分析和KEGG通路富集分析,并使用qRT-PCR对测序结果的准确性进行验证。结果模拟高原环境导致小鼠神经元损伤,并且这种损伤伴随脑血管平滑肌收缩功能改变;RNA-seq测序结果显示,Hypoxia组和Control组相比有511个DEGs(变化大于2倍;P<0.05),其中298个上调差异基因,213个下调差异基因。GO富集分析DEGs主要富集在线粒体结构功能以及糖代谢相关途径。在KEGG通路富集分析中,糖酵解/糖异生、氨基酸生物合成、RIG-I样受体信号通路较显著(P<0.05)。结论CVSMCs可通过改变能量代谢、物质代谢、信号传导等多个途径在低气压缺氧脑损伤中发挥作用。本研究为后续进一步研究CVSMCs在低气压缺氧脑损伤中的作用机制提供了新的方向和思路。

关键词：脑血管平滑肌细胞低气压缺氧脑损伤 RNA-seq GO富集分析 KEGG通路富集分析

左归丸含药血清对成骨细胞中Ihh、Ptch、Smo、GLi1蛋白表达及破骨细胞数量的影响

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中国中医基础医学杂志 2024年第6期30卷 998-1004页

作者：史婧儒廖泽绮王雨荷赵宏艳张元月刘梅洁中国中医科学院医学实验中心北京100700

目的探讨左归丸含药血清对成骨细胞中Hedgehog-GLi通路关键蛋白表达及破骨细胞数量的影响。方法选用50只雌性SD大鼠,随机分为空白对照组、假手术组、造模组。造模组大鼠切除双侧卵巢,12周后随机分为卵巢切除组(ovariectomy,OVX)、阳... 详细信息

目的探讨左归丸含药血清对成骨细胞中Hedgehog-GLi通路关键蛋白表达及破骨细胞数量的影响。方法选用50只雌性SD大鼠,随机分为空白对照组、假手术组、造模组。造模组大鼠切除双侧卵巢,12周后随机分为卵巢切除组(ovariectomy,OVX)、阳性对照组和左归丸组。阳性对照组大鼠灌服戊酸雌二醇混悬液,左归丸组大鼠灌服左归丸水煎液,连续干预7 d。末次给药1.5 h后,腹主动脉取血制备各组相应含药血清。建立成骨细胞、破骨细胞、骨磨片共培养体系,分为空白对照血清组、假手术血清组、OVX血清组、阳性对照血清组、左归丸含药血清组、激动剂组和激动剂+左归丸含药血清组,分别添加各组对应血清。免疫荧光法检测成骨细胞印度刺猬蛋白(Indian hedgehog,Ihh)、碎片蛋白(patched,Ptch)、润滑蛋白(smoothened,Smo)及GLi1蛋白表达;免疫组化法检测成骨细胞骨保护蛋白(osteoprotegerin,OPG)、核因子受体激活因子-κB配体(receptor activator for nuclear factor-κB ligand,RANKL)蛋白表达;对破骨细胞进行TRAP染色,计算TRAP+细胞数;ELISA检测共培养体系上清液中TRAP的含量。结果与假手术血清组比较,OVX血清组Ihh、Ptch、Smo、GLi1蛋白表达水平显著上调(P<0.01),RANKL/OPG比值增高;TRAP+细胞数和细胞上清液中TRAP含量亦明显增加(P<0.05)。与OVX血清组比较,左归丸含药血清组Ihh、Ptch、Smo、GLi1的蛋白表达明显下调,RANKL/OPG比值下降;TRAP+细胞数和细胞上清液中TRAP含量明显降低(P<0.05)。与激动剂组比较,加入左归丸含药血清后,Ihh、Ptch、Smo、GLi1的蛋白表达及RANKL/OPG比值均明显下降,TRAP+细胞个数及TRAP含量亦明显降低(P<0.05)。结论左归丸含药血清可通过抑制异常活跃的Hedgehog-GLi信号通路,使Ihh、Ptch、Smo、GLi1的蛋白表达水平下调,降低RANKL/OPG比值,抑制破骨细胞的数量和活性,起到减少骨丢失作用。

关键词：左归丸骨质疏松症 Hedgehog-GLi信号通路成骨细胞破骨细胞

Assessment of C-band compact polarimetry SAR for sea ice classification

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Acta Oceanologica Sinica 2016年第5期35卷 79-88页

作者： ZHANG Xi ZHANG Jie liu meijie MENG Junmin First Institute of Oceanography State Oceanic Administration College of Physics Qingdao University

The C-band synthetic aperture radar（SAR） data from the Bohai Sea of China, the Labrador Sea in the Arctic and the Weddell Sea in the Antarctic are used to analyze and discuss the sea ice full polarimetric information reconstruction ability under compact polarimetric modes. The type of compact polarimetric mode which has the highest reconstructed accuracy is analyzed, along with the performance impact of the reconstructed pseudo quad-pol SAR data on the sea ice detection and sea ice classification. According to the assessment and analysis, it is recommended to adopt the CTLR mode for reconstructing the polarimetric parameters σ_（HH）~0,σ_（VV）~0, H and α,while for reconstructing the polarimetric parameters σ_（HV）~0, ρ_（H-V）, λ_1 and λ_2, it is recommended to use the π/4 ***, it is recommended to use the π/4 mode in studying the action effects between the electromagnetic waves and sea ice, but it is recommended to use the CTLR mode for studying the sea ice classification.

关键词： sea ice SAR compact polarimetry sea ice classification assessment and analysis

Mechanisms of ＂kidney governing bones＂ theory in traditional Chinese medicine

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Frontiers of Medicine 2014年第3期8卷 389-393页

作者： Dahong Ju meijie liu Hongyan Zhao Jun Wang Institute of Basic Theory China Academy of Traditional Chinese Medicine Beijing 100700 China

Studies conducted by our group on the mechanism of ＂kidney governing bones＂ theory in traditional Chinese medicine （TCM） are reviewed in this paper. Conclusions can be summarized as follows. （1） Neuroendocrine-immune network （NIN）-osteoclast regulatory pathway OPG-RANKL-RANK is one of the mechanisms of ＂kidney governing bones.＂ Although kidney-reinforcing therapy is regarded as one of the holistic regulatory mechanisms of the body, characteristic holistic regulation in TCM can be reflected through nonselective regulation of the NIN during kidney reinforcement therapy, which can be used to treat osteoporosis through microadjustments in the microenvironment of the bone marrow. （2） Marrow exhaustion in TCM, which is the state wherein lipocytes in the bone marrow increase whereas other cells decrease, serves as the pathogenesis of osteoporosis brought about by failure of the ＂kidney governing bones.＂（3） The kidney in TCM can be regarded as a complex system comprising multiple functional units in the body, including the unit ＂governing bones.＂ Kidney deficiency refers to a deficiency in only one or more units of the kidney system and not the whole system itself, which explains the kidney-reinforcing effect of many herbs; some herbs can treat osteoporosis, but some cannot. Although both classified as kidney-reinforcing agents, the former can resolve failure of the ＂kidney governing bones＂ unit while the latter regulates the failure of other units in the kidney system. Despite the current understanding on ＂kidney governing bones＂ theory, the mechanism of ＂kidney governing bones＂ remains complicated and unresolved. Thus, further studies in this area are warranted.

关键词： kidney governing bones kidney deficiency marrow osteoporosis neuroendocrine-immune network osteoclastregulatory pathway

Shifting the Paradigm:A Fresh Look at Physical Activity Frequency and Its Impact on Mental Health,Life Satisfaction,and Self-Rated Health in Adolescents

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International Journal of Mental Health Promotion 2024年第2期26卷 83-92页

作者： Wenjie Li Yucheng Gao Guoqing liu Rongkai Hao meijie Zhang Xiaotian Li Country School of Recreation and Community Sport Capital University of Physical Education and SportsBeijing100191China Institute of Physical Education and Training Capital University of Physical Education and SportsBeijing100191China

As adolescent mental health problems are becoming a more serious issue globally,this paper explores the relationship of physical activity in adolescents and its frequency on mental health as well as examines the mediating effects of life satisfaction and self-rated health in order to provide a reference for the promotion of mental health in adolescents.A sample of 3578 Chinese high school students completed questionnaires assessing their mental health,physical activity frequency,life satisfaction,and self-rated *** mean SCL-90 value for adolescents was found to be 1.629%,and 24.73%of adolescents had varying degrees of mental health *** physical activity frequency is positively associated with improved mental health(p<0.001).Additionally,life satisfaction and self-rated health were found to play significant mediating roles in the relationship between physical activity frequency and mental ***,low-frequency physical activity had the most pronounced mediating effect on mental health through life satisfaction,while high-frequency physical activity exerted the most significant mediating effect on mental health through self-rated *** findings underscore the importance of promoting physical activity among adolescents and highlight the distinct pathways through which physical activity frequency can influence mental health *** research is needed to explore these relationships in diverse populations and settings,as well as to develop targeted intervention.

关键词： Adolescents physical activity mental health life satisfaction self-rated health

PCA-based sea-ice image fusion of optical data by HIS transform and SAR data by wavelet transform

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Acta Oceanologica Sinica 2015年第3期34卷 59-67页

作者： liu meijie DAI Yongshou ZHANG Jie ZHANG Xi MENG Junmin XIE Qinchuan China University of Petroleum (Huadong) First Institute of Oceanography State Oceanic Administration Qingdao University

Sea ice as a disaster has recently attracted a great deal of attention in China. Its monitoring has become a routine task for the maritime sector. Remote sensing, which depends mainly on SAR and optical sensors, has become the primary means for sea-ice research. Optical images contain abundant sea-ice multi-spectral in-formation, whereas SAR images contain rich sea-ice texture information. If the characteristic advantages of SAR and optical images could be combined for sea-ice study, the ability of sea-ice monitoring would be im-proved. In this study, in accordance with the characteristics of sea-ice SAR and optical images, the transfor-mation and fusion methods for these images were chosen. Also, a fusion method of optical and SAR images was proposed in order to improve sea-ice identification. Texture information can play an important role in sea-ice classification. Haar wavelet transformation was found to be suitable for the sea-ice SAR images, and the texture information of the sea-ice SAR image from Advanced Synthetic Aperture Radar （ASAR） loaded on ENVISAT was documented. The results of our studies showed that, the optical images in the hue-intensi-ty-saturation （HIS） space could reflect the spectral characteristics of the sea-ice types more efficiently than in the red-green-blue （RGB） space, and the optical image from the China-Brazil Earth Resources Satellite （CBERS-02B） was transferred from the RGB space to the HIS space. The principal component analysis （PCA） method could potentially contain the maximum information of the sea-ice images by fusing the HIS and texture images. The fusion image was obtained by a PCA method, which included the advantages of both the sea-ice SAR image and the optical image. To validate the fusion method, three methods were used to evaluate the fused image, i.e., objective, subjective, and comprehensive evaluations. It was concluded that the fusion method proposed could improve the ability of image interpretation and sea-ice ident

关键词： sea ice optical remote sensing image SAR remote sensing image HIS transform wavelet transform PCA method