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基质辅助激光解吸电离飞行时间质谱仪检测耐碳青霉烯肺炎克雷伯菌的价值

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实用医技杂志 2024年第6期31卷 409-413,F0003页

作者：吕辉范明琴陈美红陈宇航卢丹凤蔡鹏威福建省立医院南院检验科福州350028

目的评估基质辅助激光解吸电离飞行时间质谱仪(MALDITOF MS)检测耐碳青霉烯肺炎克雷伯菌(CRKP)的价值。方法收集2020年1月至2023年1月我院住院患者分离的肺炎克雷伯菌108株,以亚胺培南或美罗培南卡片筛选出CRKP和碳青霉烯酶敏感肺炎克... 详细信息

目的评估基质辅助激光解吸电离飞行时间质谱仪(MALDITOF MS)检测耐碳青霉烯肺炎克雷伯菌(CRKP)的价值。方法收集2020年1月至2023年1月我院住院患者分离的肺炎克雷伯菌108株,以亚胺培南或美罗培南卡片筛选出CRKP和碳青霉烯酶敏感肺炎克雷伯菌(CSKP),采用CLSIm100S27标准判断CRKP的药敏,采用聚合酶链反应(PCR)检测CRKP的耐药基因序列,采用MALDITOF MS检测CRKP和CSKP特征峰。以同期收集的100株肺炎克雷伯菌,对CRKP和CSKP的特征峰进行验证。结果108株肺炎克雷伯菌中25株CRKP和83株CSKP。25株CRKP均对碳青霉烯类、头孢菌素类氨曲南、喹诺酮类抗生素和氨基糖苷类抗生素存在不同程度的耐药性。83株CSKP几乎对所有类型的抗生素敏感。25株CRKP检出携带碳青霉烯酶基因25株,测序结果显示,携带blaKPC基因20株(80.0%),携带blaNDM基因3株(12.0%),携带bla OXA48基因2株(8.00%),未检出blaGES、blaIMP、blaNDM、blaVIM。CRKP的特征峰分别为4159.5 m/z、8369.7 m/z、10808.0 m/z,CSKP的特征峰分别为3599.6 m/z、10087.5 m/z。将同期收集的100株肺炎克雷伯菌进行验证,30株CRKP中,MALDITOF MS检测出28株CRKP,70株CSKP中,MALDITOF MS检测68株CSKP,MALDITOF MS检出准确度为96.0%(96/100)。结论本地区CRKP的耐药基因多为blaKPC,MALDITOF MS可快速检测CRKP,CRKP的特征峰分别为4159.5 m/z、8369.7 m/z、10808.0 m/z。

关键词：光谱法,质量,基质辅助激光解吸电离聚合酶链反应耐碳青霉烯类肺炎克雷伯菌

探讨在糖尿病诊断中采用血清C肽以及糖化血红蛋白的效果

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糖尿病新世界 2024年第1期27卷 54-56,60页

作者：王思敏陈梅红王艺娜张淑莲郭勤华联勤保障部队第910医院检验科福建泉州362000 福建省南安市医院检验科福建南安362300

目的探讨在糖尿病诊断中血清C肽以及糖化血红蛋白(Glycosylated Haemoglobin, HbA1c)的效果。方法选取2022年1月—2023年5月联勤保障部队第910医院收治的96例糖尿病患者为糖尿病组,并选取接受体检的60名健康人员为对照组,均开展血清C... 详细信息

目的探讨在糖尿病诊断中血清C肽以及糖化血红蛋白(Glycosylated Haemoglobin, HbA1c)的效果。方法选取2022年1月—2023年5月联勤保障部队第910医院收治的96例糖尿病患者为糖尿病组,并选取接受体检的60名健康人员为对照组,均开展血清C肽与HbA1c检测,比较两组检测结果。结果与对照组比较,糖尿病组血清C肽更低,HbA1c水平更高,差异有统计学意义(P均<0.05)。血清C肽联合HbA1c诊断糖尿病的准确率为87.18%,灵敏度为85.42%,特异度为90.00%。结论糖尿病诊断中血清C肽以及HbA1c可取得较好的效果,同时可为糖尿病患者的病情评估提供参考价值。

关键词：糖尿病血清C肽糖化血红蛋白诊断

The role of CCDC12 in cell proliferation

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The role of CCDC12 in cell proliferation

第八届全国医学生物化学与分子生物学第五届全国临床应用生...

作者： Sichao Guo meihong chen State Key Laboratory of Medical Molecular Biology School of Basic MedicinePeking Union Medical CollegeInstitute of Basic Medical SciencesChinese Academy of Medical Sciences

Objective:To evaluate the role of CCDC12 in the regulation of cell ***:CCDC12 variant-1 and variant-2 were cloned from human erythroleukemia K-562 *** blot was used to detect the protein level of *** level was measured by quantitative real-time *** proliferation was evaluated by MTS *** staining was used to determine the cellular localization of the proteins,and the cell apoptosis was measured by Flow cytometry analysis with Annexin V-FITC Apoptosis Detection ***:CCDC12 variant-1 encodes isoform-1 of 19 KD,and variant-2 encoded isoform-2 is slightly smaller than ***12 was expressed differentially in different types of human ***12 protein mainly localized in the nucleus,only a small amount of CCDC12 protein was observed in the ***-expression of CCDC12 variant-2 accelerated the proliferation of K-562 cell *** role of CCDC12 was affected by its expression *** CCDC12 was high expressed,the cell proliferation was *** of CCDC12 reduced the proliferation of K-562 cell and human epithelial cervical cancer cell line ***,flow cytometry analysis result excluded the possibility that the reduction of cells was caused by ***:Knockdown of CCDC12 reduced cell proliferation in several cell lines and over-expression of CCDC12 accelerated the proliferation of K-562 cell line,suggesting that CCDC12 might play a role in cell proliferation.

关键词： CCDC12 cell proliferation

Translational regulation inhibits AXIIR-induced apoptosis

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Translational regulation inhibits AXIIR-induced apoptosis

第八届全国医学生物化学与分子生物学第五届全国临床应用生...

作者： Lijuan Kong meihong chen State Key Laboratory of Medical Molecular Biology School of Basic MedicinePeking Union Medical CollegeInstitute of Basic Medical SciencesChinese Academy of Medical Sciences

Objective:Investigate AXllR-induced apoptosis and the translational regulation of AXIIR ***:Annexin V(+) apoptotic cells were detected by flow cytometry ananlysis with Annexin V-FITC Apoptosis Detection *** distribution of AXIIR was determined by immunofluorescence *** interaction between AXIIR and Caspase-8 was confirmed by *** mRNA level was either semi-quantified by reverse transcription-PCR,or measured by quantitative real-time *** level was checked by Western ***:We found that over-expression of AXIIR induced apoptosis,and this phenomenon was found in multiple human cell *** was located in cytoplasm,bound to and activated pro-Caspase-8,which subsequently activated Caspase-3/*** induced apoptosis independent of Annexin *** was not required for TRAIL-induced Caspase-8 *** the mRNA level of AXIIR in multiple cell types was considerably high,the AXIIR protein could hardly be detected in these *** of protein degradation pathways did not elevate AXIIR protein *** translation of AXIIR was regulated by its5’-untranslated regions(5’-UTR).There are four upstream open reading frames(uORF) in the5’-UTR of AXIIR mRNA,which may contribute to the translational regulation of ***:Taken together,our observations reveal that besides being a cell surface receptor of Annexin II,AXIIR could also be located in cytoplasm and acts as a novel inducer of apoptosis in human cells,partially through activating Caspase-8 in a manner that is different from conventional apoptotic *** translation of AXIIR is generally tightly inhibited in *** physiological significance of such inhibition might be to prevent cells from apoptosis.

关键词： AXIIR Apoptosis Caspase-8 5’-UTR uORF

基于改进天牛须搜索优化SVM的车辆变速箱故障诊断

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机械传动 2022年第6期46卷 154-160页

作者：乔文山华晋陈美宏浙江经济职业技术学院汽车技术学院浙江杭州310018 南京林业大学机械电子工程学院江苏南京210037 南京林业大学工程培训中心江苏南京210037

针对支持向量机(SVM)在车辆变速箱故障诊断中性能受参数影响较大的实际,在研究天牛须搜索(BAS)的基础上,提出基于改进天牛须搜索(IBAS)优化SVM的车辆变速箱故障诊断新方法。相比于BAS,IBAS对步长公式进行了修正,同时增加了高斯变异行为... 详细信息

针对支持向量机(SVM)在车辆变速箱故障诊断中性能受参数影响较大的实际,在研究天牛须搜索(BAS)的基础上,提出基于改进天牛须搜索(IBAS)优化SVM的车辆变速箱故障诊断新方法。相比于BAS,IBAS对步长公式进行了修正,同时增加了高斯变异行为,使得算法前后期的搜索能力得到了平衡且具有跳出局部最优的能力,能够获得更优的SVM参数。车辆变速箱故障诊断结果表明,所提方法有效提高了故障诊断的精度,相比于其他几种方法效果更优。

关键词：天牛须搜索支持向量机变速箱故障诊断

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1980-2020年松嫩平原生态脆弱性时空分异

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水土保持通报 2023年第4期43卷 366-374页

作者：宋春威相恒星陈美宏杜会石吉林师范大学旅游与地理科学学院吉林四平136000 吉林省生态环境遥感大数据重点实验室吉林四平136000 中国科学院东北地理与农业生态研究所吉林长春130102

[目的]探究1980—2020年松嫩平原生态脆弱性时空变化特征和驱动机制,为该区生态系统管理与可持续发展提供科学依据。[方法]从自然和人文两个角度出发,采用“压力—状态—响应”(PSR)模型构建评价指标体系,结合主成分分析法和Moran’I指... 详细信息

[目的]探究1980—2020年松嫩平原生态脆弱性时空变化特征和驱动机制,为该区生态系统管理与可持续发展提供科学依据。[方法]从自然和人文两个角度出发,采用“压力—状态—响应”(PSR)模型构建评价指标体系,结合主成分分析法和Moran’I指数开展研究。[结果]①松嫩平原生态脆弱性以极度、重度和中度脆弱性为主,共占81.72%。2000年之前生态脆弱性整体水平呈现上升趋势,2000年之后生态脆弱性整体水平不断下降。②松嫩平原生态脆弱性以轻度和中度脆弱性变化为主,变幅分别为62.19%和91.30%。③生态脆弱性变化是由于草地、水域、未利用地、建筑用地和耕地间的转化。④生态脆弱性存在空间自相关性,低—低集聚主要分布在研究区西部林地区域。[结论]近40 a松嫩平原生态脆弱性总体呈现波动降低趋势,空间上生态脆弱性自东向西逐渐增加,气温、降水等自然因素和土地利用等人文因素对生态脆弱性影响较大。

关键词：生态脆弱性空间自相关空间主成分分析生态环境松嫩平原

Role of TXNDC5 in multiple myeloma

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Role of TXNDC5 in multiple myeloma

第八届全国医学生物化学与分子生物学第五届全国临床应用生...

作者： Hongfei Zhang Lijuan Kong Ning Zhu meihong chen State Key Laboratory of Medical Molecular Biology School of Basic MedicinePeking Union Medical CollegeInstitute of Basic Medical SciencesChinese Academy of Medical Sciences

Objective:Multiple myeloma is a plasma cell malignancy which generally develops destructive osteolytic bone *** thiored oxindomain containing five(TXNDC5) is a protein-disulfide isomerase of thioredoxin *** study aimed at understanding the role of TXNDC5 in multiple ***:Western blot was used to detect the protein level of *** proliferation was evaluated by MTS *** was applied to analyze the amount of secreted Lambda light chain in multiple myeloma ***:The result showed that the protein level of TXNDC5 was much higher in multiple myeloma cell lines *** and U266 B1 than in RPMI18226 *** differential expression suggested that TXNDC5 may be involved in the phenotypes of multiple myeloma *** of TXNDC5 by specific siRNA reduced both cell proliferation and the secretion of Lambda light chain in MM.1 S ***:The above results indicate that TXNDC5 is probably involved in the development of multiple myeloma.

关键词： TXNDC5 multiple myeloma

Long noncoding RNA induces erythroid differentiation

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Long noncoding RNA induces erythroid differentiation

第八届全国医学生物化学与分子生物学第五届全国临床应用生...

作者： Chuan Qiu Jiewen Zhang Ning Zhu meihong chen State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical College

Erythroid differentiation is a tightly regulated multi-step process that has not been fully *** noncoding RNAs(IncRNAs)are emerging as important regulators of cellular process,such as development,differentiation,and *** present study aimed to explore the role of a newly defined long noncoding RNA KZJ in erythroid *** cloned two

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单采血小板和浓缩血小板在不同输注策略下的疗效分析

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中国输血杂志 2022年第6期35卷 615-618页

作者：陈梅红黄家福解放军联勤保障部队第910医院输血科福建泉州362000

目的回顾性分析单采血小板和浓缩血小板在不同输注策略下的临床效果,探索更加科学的输血方式。方法收集2020年~2021年期间本院收治的血小板减少症患者279例,按输注策略将其分为A组(单独输注单采血小板),B组(单独输注浓缩血小板),C组(单... 详细信息

目的回顾性分析单采血小板和浓缩血小板在不同输注策略下的临床效果,探索更加科学的输血方式。方法收集2020年~2021年期间本院收治的血小板减少症患者279例,按输注策略将其分为A组(单独输注单采血小板),B组(单独输注浓缩血小板),C组(单采血小板和浓缩血小板同时输注)3组,测量3组患者输注前后24 h内的血小板并计算其CCI值,比较3组患者间CCI值,输注有效率及输血不良反应的差别,以及不同血型下3组间的疗效差别。结果单采血小板单独输注的患者输注有效率更高,输血不良反应率更低,但输注疗效较低,仅为(14.63±13.48)(P<0.05);对比浓缩血小板单独输注的患者输注疗效可达(16.00±21.77),但输血有效率较低,输血不良反应率大幅升高;二者同时输注的患者则可以在保持输注疗效(16.21±16.33)的情况下大幅提高输注有效率,降低输血不良反应率。同时不同血型对血小板的输注疗效也存在影响。结论单采血小板和浓缩血小板同时输注的患者可以在保证输注量的情况下有效提高患者的输注疗效,降低输血不良反应率,是具备一定可行性及安全性的输血策略。

关键词：血小板减少症单采血小板浓缩血小板 CCI