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大黄䗪虫丸联合戈舍瑞林治疗子宫内膜异位症临床观察

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中国中医药现代远程教育 2024年第22期22卷 167-169页

作者：陈美红张晓玲江西省妇幼保健院妇科江西南昌330006

目的探讨大黄䗪虫丸联合戈舍瑞林治疗子宫内膜异位症的临床效果及对患者生活质量的影响。方法选择在江西省妇幼保健院住院行腹腔镜手术的子宫内膜异位症患者随机分为两组,各60例。对照组予醋酸戈舍瑞林缓释植入剂,研究组在此基础上予大黄... 详细信息

目的探讨大黄䗪虫丸联合戈舍瑞林治疗子宫内膜异位症的临床效果及对患者生活质量的影响。方法选择在江西省妇幼保健院住院行腹腔镜手术的子宫内膜异位症患者随机分为两组,各60例。对照组予醋酸戈舍瑞林缓释植入剂,研究组在此基础上予大黄䗪虫丸,观察两组性激素水平、视觉模拟量表(VAS)评分、生存质量评价量表(SF-36)评分。结果治疗后,两组性激素水平及VAS评分均降低、SF-36评分均提高,且相对于对照组,研究组各指标改善情况更明显(P<0.05),患者生活质量得到明显改善。结论大黄䗪虫丸联合戈舍瑞林应用于子宫内膜异位症患者,存在相互补充作用,能够明显改善其疼痛症状,提高生活质量,彰显了中西医药结合的综合治疗优势,临床取得了较为满意的效果,值得推广应用。

关键词：子宫内膜异位症大黄䗪虫丸戈舍瑞林中西医结合疗法

灌木辣椒(Capsicum frutescens)抗象耳豆根结线虫的组织学及生理生化特征分析

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热带作物学报 2024年第9期45卷 1902-1911页

作者：陈梅红黄银香林珑高畅涂容生朱婕刘子记海南大学热带农林学院(农业农村学院、乡村振兴学院) 海南海口570228 热带特色林木花卉遗传与种质创新教育部重点实验室海南海口570228 中国热带农业科学院热带作物品种资源研究所海南海口571101

象耳豆根结线虫(Meloidogyne enterolobii)寄主范围广,侵染能力强,已发展为根结线虫(Meloidogyne spp.)中最具破坏性的1个种。海南地区辣椒生产受象耳豆根结线虫影响较大。利用作物本身的抗性来防治该病害是比较绿色、经济、环保的办法... 详细信息

象耳豆根结线虫(Meloidogyne enterolobii)寄主范围广,侵染能力强,已发展为根结线虫(Meloidogyne spp.)中最具破坏性的1个种。海南地区辣椒生产受象耳豆根结线虫影响较大。利用作物本身的抗性来防治该病害是比较绿色、经济、环保的办法。前期,本研究团队已从30份本地灌木辣椒(Capsicumfrutescens)资源中筛选出1份抗病种质CF25。为更好地利用该种质开展抗病育种工作,加深对其抗病机理的理解,本研究对抗病种质CF25和感病种质CF29接种象耳豆根结线虫,通过酸性品红染色和石蜡切片等方法,结果发现在根结线虫侵入量方面,CF25的线虫侵入量比CF29少;在根结线虫发育形态方面,CF25中线虫发育比CF29缓慢,且出现雌虫发育不良的现象;在根系解剖结构方面,CF25巨细胞中的内容物逐渐减少,出现空洞化现象。为初步探索CF25抗根结线虫的生理基础,对CF25和CF29进行过氧化物酶(POD)和苯丙氨酸解氨酶(PAL)活性测定。结果显示,接种后多数时间点CF25的POD和PAL活性显著高于CF29。综上所述,从组织学角度来看,灌木辣椒资源CF25对象耳豆根结线虫的抗性主要体现在抗侵入和限制取食两方面;CF25巨细胞内容物的减少可能抑制了根结线虫的进食,从而导致其发育缓慢且不良;从生理生化角度来看,CF25较高的POD活性可能有利于清除根结线虫侵染导致的活性氧积累。此外,较高的POD和PAL活性可能导致包括木质素在内的更多苯丙烷类次生代谢产物的生成,从而提高了CF25的抗病性。本研究分析了灌木辣椒资源CF25抗象耳豆根结线虫的组织学和生理生化基础,可为后续深入探究灌木辣椒抗象耳豆根结线虫机理提供依据。

关键词：象耳豆根结线虫抗性灌木辣椒组织学 POD PAL

基质辅助激光解吸电离飞行时间质谱仪检测耐碳青霉烯肺炎克雷伯菌的价值

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实用医技杂志 2024年第6期31卷 409-413,F0003页

作者：吕辉范明琴陈美红陈宇航卢丹凤蔡鹏威福建省立医院南院检验科福州350028

目的评估基质辅助激光解吸电离飞行时间质谱仪(MALDITOF MS)检测耐碳青霉烯肺炎克雷伯菌(CRKP)的价值。方法收集2020年1月至2023年1月我院住院患者分离的肺炎克雷伯菌108株,以亚胺培南或美罗培南卡片筛选出CRKP和碳青霉烯酶敏感肺炎克... 详细信息

目的评估基质辅助激光解吸电离飞行时间质谱仪(MALDITOF MS)检测耐碳青霉烯肺炎克雷伯菌(CRKP)的价值。方法收集2020年1月至2023年1月我院住院患者分离的肺炎克雷伯菌108株,以亚胺培南或美罗培南卡片筛选出CRKP和碳青霉烯酶敏感肺炎克雷伯菌(CSKP),采用CLSIm100S27标准判断CRKP的药敏,采用聚合酶链反应(PCR)检测CRKP的耐药基因序列,采用MALDITOF MS检测CRKP和CSKP特征峰。以同期收集的100株肺炎克雷伯菌,对CRKP和CSKP的特征峰进行验证。结果108株肺炎克雷伯菌中25株CRKP和83株CSKP。25株CRKP均对碳青霉烯类、头孢菌素类氨曲南、喹诺酮类抗生素和氨基糖苷类抗生素存在不同程度的耐药性。83株CSKP几乎对所有类型的抗生素敏感。25株CRKP检出携带碳青霉烯酶基因25株,测序结果显示,携带blaKPC基因20株(80.0%),携带blaNDM基因3株(12.0%),携带bla OXA48基因2株(8.00%),未检出blaGES、blaIMP、blaNDM、blaVIM。CRKP的特征峰分别为4159.5 m/z、8369.7 m/z、10808.0 m/z,CSKP的特征峰分别为3599.6 m/z、10087.5 m/z。将同期收集的100株肺炎克雷伯菌进行验证,30株CRKP中,MALDITOF MS检测出28株CRKP,70株CSKP中,MALDITOF MS检测68株CSKP,MALDITOF MS检出准确度为96.0%(96/100)。结论本地区CRKP的耐药基因多为blaKPC,MALDITOF MS可快速检测CRKP,CRKP的特征峰分别为4159.5 m/z、8369.7 m/z、10808.0 m/z。

关键词：光谱法,质量,基质辅助激光解吸电离聚合酶链反应耐碳青霉烯类肺炎克雷伯菌

探讨在糖尿病诊断中采用血清C肽以及糖化血红蛋白的效果

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糖尿病新世界 2024年第1期27卷 54-56,60页

作者：王思敏陈梅红王艺娜张淑莲郭勤华联勤保障部队第910医院检验科福建泉州362000 福建省南安市医院检验科福建南安362300

目的探讨在糖尿病诊断中血清C肽以及糖化血红蛋白(Glycosylated Haemoglobin, HbA1c)的效果。方法选取2022年1月—2023年5月联勤保障部队第910医院收治的96例糖尿病患者为糖尿病组,并选取接受体检的60名健康人员为对照组,均开展血清C... 详细信息

目的探讨在糖尿病诊断中血清C肽以及糖化血红蛋白(Glycosylated Haemoglobin, HbA1c)的效果。方法选取2022年1月—2023年5月联勤保障部队第910医院收治的96例糖尿病患者为糖尿病组,并选取接受体检的60名健康人员为对照组,均开展血清C肽与HbA1c检测,比较两组检测结果。结果与对照组比较,糖尿病组血清C肽更低,HbA1c水平更高,差异有统计学意义(P均<0.05)。血清C肽联合HbA1c诊断糖尿病的准确率为87.18%,灵敏度为85.42%,特异度为90.00%。结论糖尿病诊断中血清C肽以及HbA1c可取得较好的效果,同时可为糖尿病患者的病情评估提供参考价值。

关键词：糖尿病血清C肽糖化血红蛋白诊断

The role of CCDC12 in cell proliferation

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The role of CCDC12 in cell proliferation

第八届全国医学生物化学与分子生物学第五届全国临床应用生物化学与分子生物学2013华东六省一市生物化学与分子生物学联合学术研讨会

作者： Sichao Guo meihong chen State Key Laboratory of Medical Molecular Biology School of Basic MedicinePeking Union Medical CollegeInstitute of Basic Medical SciencesChinese Academy of Medical Sciences

Objective:To evaluate the role of CCDC12 in the regulation of cell ***:CCDC12 variant-1 and variant-2 were cloned from human erythroleukemia K-562 *** blot was used to detect the protein level of *** level was measured by quantitative real-time *** proliferation was evaluated by MTS *** staining was used to determine the cellular localization of the proteins,and the cell apoptosis was measured by Flow cytometry analysis with Annexin V-FITC Apoptosis Detection ***:CCDC12 variant-1 encodes isoform-1 of 19 KD,and variant-2 encoded isoform-2 is slightly smaller than ***12 was expressed differentially in different types of human ***12 protein mainly localized in the nucleus,only a small amount of CCDC12 protein was observed in the ***-expression of CCDC12 variant-2 accelerated the proliferation of K-562 cell *** role of CCDC12 was affected by its expression *** CCDC12 was high expressed,the cell proliferation was *** of CCDC12 reduced the proliferation of K-562 cell and human epithelial cervical cancer cell line ***,flow cytometry analysis result excluded the possibility that the reduction of cells was caused by ***:Knockdown of CCDC12 reduced cell proliferation in several cell lines and over-expression of CCDC12 accelerated the proliferation of K-562 cell line,suggesting that CCDC12 might play a role in cell proliferation.

关键词： CCDC12 cell proliferation

Translational regulation inhibits AXIIR-induced apoptosis

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Translational regulation inhibits AXIIR-induced apoptosis

第八届全国医学生物化学与分子生物学第五届全国临床应用生物化学与分子生物学2013华东六省一市生物化学与分子生物学联合学术研讨会

作者： Lijuan Kong meihong chen State Key Laboratory of Medical Molecular Biology School of Basic MedicinePeking Union Medical CollegeInstitute of Basic Medical SciencesChinese Academy of Medical Sciences

Objective:Investigate AXllR-induced apoptosis and the translational regulation of AXIIR ***:Annexin V(+) apoptotic cells were detected by flow cytometry ananlysis with Annexin V-FITC Apoptosis Detection *** distribution of AXIIR was determined by immunofluorescence *** interaction between AXIIR and Caspase-8 was confirmed by *** mRNA level was either semi-quantified by reverse transcription-PCR,or measured by quantitative real-time *** level was checked by Western ***:We found that over-expression of AXIIR induced apoptosis,and this phenomenon was found in multiple human cell *** was located in cytoplasm,bound to and activated pro-Caspase-8,which subsequently activated Caspase-3/*** induced apoptosis independent of Annexin *** was not required for TRAIL-induced Caspase-8 *** the mRNA level of AXIIR in multiple cell types was considerably high,the AXIIR protein could hardly be detected in these *** of protein degradation pathways did not elevate AXIIR protein *** translation of AXIIR was regulated by its5’-untranslated regions(5’-UTR).There are four upstream open reading frames(uORF) in the5’-UTR of AXIIR mRNA,which may contribute to the translational regulation of ***:Taken together,our observations reveal that besides being a cell surface receptor of Annexin II,AXIIR could also be located in cytoplasm and acts as a novel inducer of apoptosis in human cells,partially through activating Caspase-8 in a manner that is different from conventional apoptotic *** translation of AXIIR is generally tightly inhibited in *** physiological significance of such inhibition might be to prevent cells from apoptosis.

关键词： AXIIR Apoptosis Caspase-8 5’-UTR uORF

Role of TXNDC5 in multiple myeloma

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Role of TXNDC5 in multiple myeloma

第八届全国医学生物化学与分子生物学第五届全国临床应用生物化学与分子生物学2013华东六省一市生物化学与分子生物学联合学术研讨会

作者： Hongfei Zhang Lijuan Kong Ning Zhu meihong chen State Key Laboratory of Medical Molecular Biology School of Basic MedicinePeking Union Medical CollegeInstitute of Basic Medical SciencesChinese Academy of Medical Sciences

Objective:Multiple myeloma is a plasma cell malignancy which generally develops destructive osteolytic bone *** thiored oxindomain containing five(TXNDC5) is a protein-disulfide isomerase of thioredoxin *** study aimed at understanding the role of TXNDC5 in multiple ***:Western blot was used to detect the protein level of *** proliferation was evaluated by MTS *** was applied to analyze the amount of secreted Lambda light chain in multiple myeloma ***:The result showed that the protein level of TXNDC5 was much higher in multiple myeloma cell lines *** and U266 B1 than in RPMI18226 *** differential expression suggested that TXNDC5 may be involved in the phenotypes of multiple myeloma *** of TXNDC5 by specific siRNA reduced both cell proliferation and the secretion of Lambda light chain in MM.1 S ***:The above results indicate that TXNDC5 is probably involved in the development of multiple myeloma.

关键词： TXNDC5 multiple myeloma

Long noncoding RNA induces erythroid differentiation

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Long noncoding RNA induces erythroid differentiation

第八届全国医学生物化学与分子生物学第五届全国临床应用生物化学与分子生物学2013华东六省一市生物化学与分子生物学联合学术研讨会

作者： Chuan Qiu Jiewen Zhang Ning Zhu meihong chen State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical College

Erythroid differentiation is a tightly regulated multi-step process that has not been fully *** noncoding RNAs(IncRNAs)are emerging as important regulators of cellular process,such as development,differentiation,and *** present study aimed to explore the role of a newly defined long noncoding RNA KZJ in erythroid *** cloned two

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The Genomes of the Allohexaploid Echinochloa crus-galli and Its Progenitors Provide Insights into Polyploidization-Driven Adaptation

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Molecular Plant 2020年第9期13卷 1298-1310页

作者： Chu-Yu Ye Dongya Wu Lingfeng Mao Lei Jia Jie Qiu Sangting Lao meihong chen Bowen Jiang Wei Tang Qiong Peng Lang Pan Lifeng Wang Xiaoxiao Feng Longbiao Guo Chulong Zhang Elizabeth A.Kellogg Kenneth M.Olsen Lianyang Bai Longjiang Fan Institute of Crop Sciences&Institute of Bioinformatics Zhejiang UniversityHangzhou 310058China Shanghai Key Laboratory of Plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200235China State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006China Hunan Weed Science Key Laboratory Hunan Academy of Agriculture ScienceChangsha 410125China Agricultural Experiment Station Zhejiang UniversityHangzhou 310058China lnstitute of Biotechnology Zhejiang UniversityHangzhou 310058China Donald Danforth Plant Science Center St.LouisMO 63132USA Department of Biology Washington University in St.LouisSt.LouisMO 63130USA

The hexaploid species Echinochloa crus-galli is one of the most detrimental weeds in crop fields,especially in rice *** evolutionary history is similar to that of bread wheat,arising through polyploidization after hybridization between a tetraploid and a diploid *** this study,we generated and analyzed high-quality genome sequences of diploid(***),tetraploid(***),and hexaploid(***-galli)Echinochloa *** family analysis showed a significant loss of disease-resistance genes such as those encoding NB-ARC domain-containing proteins during Echinochloa polyploidization,contrary to their significant expansionduring wheat polyploidization,suggesting that natural selection might favor reduced investment in resistance in this weed to maximize its growth and *** contrast to the asymmetric patterns of genome evolution observed in wheat and other crops,no significant differences in selection pressure were detected between the subgenomes in *** and *** addition,distinctive differences in subgenome transcriptome dynamics during hexaploidization were observed between ***-galli and bread ***,our study documents genomic mechanisms underlying the adaptation of a major agricultural weed during *** genomic and transcriptomic resources of three Echinochloa species and new insights into the polyploidization-driven adaptive evolution would be useful for future breeding cereal crops.

关键词： breeding crops ation