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Journal of the Operations Research Society of China 2020年第4期8卷 601-617页

作者： megumi yamamoto Nobuo Umemura Hiroyuki Kawano School of Contemporary International Studies Nagoya University of Foreign StudiesIwasaki-choNisshin 470-0197Japan School of Media and Design Nagoya University of Arts and SciencesNagoya 470-0196Japan Faculty of Science and Engineering Nanzan UniversityNagoya 466-8673Japan

We are developing a Moodle plug-in,which is an AES(automated essay scoring)support system for the basic education of university *** system evaluates essays based on rubric,which has five evaluation viewpoints“Contents,Structure,Evidence,Style,and Skill”.Vocabulary level is one of the scoring items of *** is calculated using Japanese Language Learners’Dictionaries constructed by Sunakawa et *** this does not fully cover the words used in the student-level essays,we found that there is a problem with the accuracy of the vocabulary level *** this paper,we propose to construct comprehensive Japanese vocabulary difficulty level dictionaries using Japanese Wikipedia as the *** apply Latent Dirichlet Allocation(LDA)to the Wikipedia corpus and find the word appearance probability as one of the indexes of word *** use the TF-IDF value instead of the LDA value of the words,which rarely *** a result,we constructed highly comprehensive Japanese vocabulary difficulty level *** confirmed that the vocabulary level can be scored for all words in the test dataset by using the constructed dictionaries.

关键词： Automated essay scoring Vocabulary level Dictionary Wikipedia Corpus LDA Rubric

蛋白组谱揭示甲基汞暴露的普通狨猴（Callithrix jacchus）大脑区域性蛋白改变

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蛋白组谱揭示甲基汞暴露的普通狨猴（Callithrix jacchus）大脑区...

中国毒理学会第九次全国毒理学大会

作者：邵月婷 megumi yamamoto Laurie H.M Chan Department of Biology University of Ottawa 广州医科大学公共卫生学院 Department of Basic Medical Science National Institute for Minamata Disease

目的甲基汞(MeHg)是常见环境污染物及神经毒素,20世纪50年代发生在日本的水俣病是误食甲基汞污染的水产品造成的以神经系统为主要症状的公害病。成人病例主要表现为选择性的损害大脑距状裂和中央前皮质,但其具体的生物学机制尚不清楚。... 详细信息

目的甲基汞(MeHg)是常见环境污染物及神经毒素,20世纪50年代发生在日本的水俣病是误食甲基汞污染的水产品造成的以神经系统为主要症状的公害病。成人病例主要表现为选择性的损害大脑距状裂和中央前皮质,但其具体的生物学机制尚不清楚。本研究的主要目的是应用蛋白组学的方法鉴定并分析MeHg暴露下的普通狨猴大脑两个皮层区域(前额叶和枕叶包括距状沟)显著变化的蛋白表达及其下游参与的生物学功能和信号通路。材料和方法 6只普通狨猴共分两组,分别喂食MeHg(1.5 mg·kg·d溶于半胱氨酸溶液中)及半胱氨酸对照组,连续喂食两周,停药两周。麻醉后取出狨猴大脑标本,分离额叶和枕叶,应用蛋白组学技术HPLC-ESI-MS/MS(高效液相色谱-电喷雾-串联质谱)研究甲基汞(MeHg)暴露对普通狨猴额叶枕叶两区域的影响。Cytoscape及IPA平台用于狨猴大脑两区域显著变化蛋白组的聚类、富集分析以及信号通路分析。免疫印迹实验用于验证质谱预测结果。结果在MeHg给药组和对照组的大脑前叶和枕叶分别筛选出1045和1062个蛋白质,两区域受MeHg影响显著变化的蛋白分别为62和89个(P<0.05)。生物功能富集分析结果显示在狨猴大脑额叶和枕叶,脂质代谢过程(Corr. P=6.14E-112)与蛋白水解(Corr. P=9.74E-112)作用均受到影响。在额叶,细胞周期、细胞分化功能、硫化物代谢过程、微管运动、甘油脂质代谢过程为受MeHg影响的主要功能。与额叶相比,枕叶中显著变化的蛋白主要主要集中在转运功能、碳水化合物代谢过程、化学物引起的内环境稳态失衡以及体液水平调节障碍。通路富集分析结果显示MeHg主要影响额叶抗利尿激素调节的水再吸收功能,这可部分解释狨猴大脑病理观察到的皮层水肿现象。免疫印迹实验进一步证实了质谱预测的蛋白表达(DLG4,GPX1,AQP4,DYNC1LI2)结果。结论上述结果表明MeHg可诱导额叶和枕叶区域特异性蛋白发生变化,但所致两区域的终点效应(如能量受损、水转运障碍)相似。APOE和GPX1可能是MeHg引起枕叶多重功能改变的两个潜在的关键蛋白。本研究首次报道了MeHg神经毒性暴露下灵长类大脑顶叶和额叶区域性蛋白组的变化,结果将有助于更好的理解人类MeHg中毒症状及效应发生的分子机制。

关键词：甲基汞蛋白组学神经毒性普通狨猴

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甲基汞暴露对普通狨猴肾脏蛋白组的影响

甲基汞暴露对普通狨猴肾脏蛋白组的影响

Identification of telomere maintenance gene variations related to lung adenocarcinoma risk by genome-wide association and whole genome sequencing analyses

2018环境与健康学术会议--精准环境健康：跨学科合作的挑战

作者：邵月婷蒋义国董薇 megumi yamamoto Gleb Mironov Laurie H.MChan 广州医科大学公共卫生学院预防医学系加拿大渥太华大学生物系日本国家水俣病研究中心基础医学系

目的研究甲基汞暴露下普通狨猴肾脏蛋白组的变化特点,以及肾脏生物功能、细胞组分、信号转导通路及其相关蛋白的变化。方法普通狨猴(Callithrix jacchus)分别暴露于氯化甲基汞(1.5mg/kg,L-半胱氨酸),和非暴露组(给予同样体积的L-半胱氨... 详细信息

目的研究甲基汞暴露下普通狨猴肾脏蛋白组的变化特点,以及肾脏生物功能、细胞组分、信号转导通路及其相关蛋白的变化。方法普通狨猴(Callithrix jacchus)分别暴露于氯化甲基汞(1.5mg/kg,L-半胱氨酸),和非暴露组(给予同样体积的L-半胱氨酸)2周。继而停止给药2周,麻醉取材狨猴肾脏。LC-ESI-MS/MS鉴定蛋白质,两种生物信息学工具(Cytoscape与Webgestalt)对MeHg暴露和非暴露的狨猴肾脏蛋白组进行比较分析,通过搜索人源性数据库对显著变化的蛋白质进行分析。Immuno blot用于核实液相质谱结果。结果 MeHg暴露与非暴露组间蛋白质表达差异显著。共筛选出914个蛋白质,155个蛋白发生显著变化p<0.05(60个上调,29个下调)。Gene Ontology(GO)富集分析显示普通狨猴肾脏受MeHg影响的生物过程主要涉及甘油三酯生物合成过程,谷氨酰胺氨基酸生物合成、跨膜运输功能蛋白及蛋白水解(p<0.05);组分分析结果显示内吞小泡、液泡腔、质膜组分及内质网伴侣复合体为受MeHg影响的细胞器;转运蛋白活性、肽酶活性、特别是作用于L-氨基酸肽的肽酶活性、丝氨酸型内肽酶活性为受MeHg影响的分子功能(p<0.05)。KEGG信号通路富集分析结果显示肾脏受MeHg影响的主要信号通路分别为脂肪细胞因子信号通路、甘油脂代谢、维生素代谢及精氨酸生物合成(p<0.05)。Immunoblot结果显示与脂类代谢相关的ACLY蛋白,及抗氧化应激GPX-1蛋白含量的变化与质谱预测结果一致。结论 MeHg主要通过诱导氧化应激、引起脂质过氧化的形式损伤肾脏。甲基汞靶向肾脏的转运系统如囊泡、质膜、内膜系统。ACLY及ACSL1蛋白为受甲基汞暴露后肾脏损伤的重要蛋白。

关键词：甲基汞肾毒性普通狨猴 LC-ESI-MS/MS(液相色谱-电喷雾串联质谱)

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Cancer Communications 2024年第2期44卷 287-293页

作者： Kouya Shiraishi Atsushi Takahashi Yukihide Momozawa Yataro Daigo Syuzo Kaneko Takahisa Kawaguchi Hideo Kunitoh Shingo Matsumoto Hidehito Horinouchi Akiteru Goto Takayuki Honda Kimihiro Shimizu Masahiro Torasawa Daisuke Takayanagi Motonobu Saito Akira Saito Yuichiro Ohe Shun-ichi Watanabe Koichi Goto Masahiro Tsuboi Katsuya Tsuchihara Sadaaki Takata Tomomi Aoi Atsushi Takano Masashi Kobayashi Yohei Miyagi Kazumi Tanaka Hiroyuki Suzuki Daichi Maeda Takumi Yamaura Maiko Matsuda Yoko Shimada Takaaki Mizuno Hiromi Sakamoto Teruhiko Yoshida Yasushi Goto Tatsuya Yoshida Taiki Yamaji Makoto Sonobe Shinichi Toyooka Kazue Yoneda Katsuhiro Masago Fumihiro Tanaka megumi Hara Nobuo Fuse Satoshi S.Nishizuka Noriko Motoi Norie Sawada Yuichiro Nishida Kazuki Kumada Kenji Takeuchi Kozo Tanno Yasushi Yatabe Kuniko Sunami Tomoyuki Hishida Yasunari Miyazaki Hidemi Ito Mitsuhiro Amemiya Hirohiko Totsuka Haruhiko Nakayama Tomoyuki Yokose Kazuyoshi Ishigaki Toshiteru Nagashima Yoichi Ohtaki Kazuhiro Imai Ken Takasawa Yoshihiro Minamiya Kazuma Kobayashi Kenichi Okubo Kenji Wakai Atsushi Shimizu Masayuki yamamoto Motoki Iwasaki Koichi Matsuda Johji Inazawa Yuichi Shiraishi Hiroyoshi Nishikawa Yoshinori Murakami Michiaki Kubo Fumihiko Matsuda Yoichiro Kamatani Ryuji Hamamoto Keitaro Matsuo Takashi Kohno Division of Genome Biology National Cancer Center Research InstituteTokyoJapan Department of Clinical Genomics National Cancer Center Research InstituteTokyoJapan Laboratory for Statistical Analysis Center for Integrative Medical SciencesRIKENYokohamaJapan Department of Genomic Medicine Research InstituteNational Cerebral and Cardiovascular CenterOsakaJapan Laboratory for Genotyping Development Center for Integrative Medical SciencesRIKENYokohamaJapan Center for Antibody and Vaccine Therapy Research HospitalInstitute of Medical ScienceThe University of TokyoTokyoJapan Department of Medical Oncology and Cancer Center and Center for Advanced Medicine against CancerShiga University of Medical ScienceOtsuJapan Division of Medical AI Research and Development National Cancer Center Research InstituteTokyoJapan Center for Genomic Medicine Graduate School of MedicineKyoto UniversityKyotoJapan Department of Medical Oncology Japanese Red Cross Medical CenterTokyoJapan Department of Thoracic Oncology National Cancer Center Hospital EastJapan Department of Thoracic Oncology National Cancer Center HospitalTokyoJapan Department of Cellular and Organ Pathology Graduate School of MedicineAkita UniversityAkitaJapan Department of Respiratory Medicine Tokyo Medical and Dental UniversityBunkyo-kuTokyoJapan Division of General Thoracic Surgery Department of SurgeryShinshu University School of MedicineMatsumotoNaganoJapan Department of General Surgical Science Division of General Thoracic SurgeryGunma University Graduate School of MedicineIntegrative Center of General SurgeryGunma University HospitalGunmaJapan Department of Gastrointestinal Tract Surgery Fukushima Medical University School of MedicineFukushimaJapan StaGen Co. LtdTokyoJapan Division of Thoracic Surgery National Cancer Center HospitalTokyoJapan Department of Thoracic Surgery National Cancer Center Hospital EastChibaJapan Division of Translational Informatics Exploratory Oncology Research and Clinical Trial Center(EPOC

Dear editor,Lung carcinoma is responsible for the highest fatal-ity rate among cancer-related deaths globally,with lung adenocarcinoma(LADC)emerging as the prevailing sub-type.

关键词： adenocarcinoma lung Lung

Identifying bereaved grievers with greatest medical or social service needs in Japan

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Family Medicine and Community Health 2022年第1期10卷 1-9页

作者： Carl Becker Yozo Taniyama megumi Kondo-Arita Noriko Sasaki Shinya Yamada Kayoko yamamoto Policy Science Unit School of MedicineKyoto UniversityKyotoJapan Department of Religious Studies Tohoku UniversitySendaiJapan Nakayama International Center Osaka Medical CollegeTakatsukiJapan Department of Healthcare Economics and Quality Management Graduate School of MedicineKyoto UniversityKyotoJapan Department of Ethnology National Museum of Japanese HistorySakuraJapan Department of Nursing Tenri Health Care UniversityTenriJapan

Objective Severe grief adversely affects the health of bereaved families,potentially burdening medical and community health *** for effective community health maintenance must identify the people likely to face severe effects of *** present study identified characteristics of mourners who experience severe grief within a year of bereavement to confirm whether this grief increased their reliance on Japanese medical and social *** We conducted a nationwide postal survey of Japanese bereaved within the previous year,to compare those reporting daily or overwhelming‘heavy’grief to those with less heavy grief,in terms of demographic and socioeconomic details,daily work and non-work activity,frequency of medical and social service ***/participants In 2019,with the support of the Ministry of Education and the All Japan Funeral Co-Operation,we distributed approximately 5500 questionnaires to Japanese who had presided at funerals within the past year for anonymous *** January of 2020,we received 1078 complete voluntary responses from bereaved *** Half of the‘heavy grief’group(n=143)reported adverse effects on health and daily life,including needs for pharmacological,medical or welfare *** of husbands or children were particularly connected to severe grief;‘unexpected’death from cancer caused the greatest ***(even part-time)buffered against severe grief;grief was greater for the unemployed and substantially worse for those who lost significant income at the same time as they lost loved *** These findings suggest that prior counselling should reduce the shock of bereavement and economic loss,which increases subsequent medical *** professionals and community health workers can use the above factors to target in advance the family members in greatest danger of heavy grief,to intervene lest grief adversely affect their physical and psychological health after ber

关键词： service sided return

Experimental antithrombotic effect of potatoes harvested in the autumn

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Health 2012年第2期4卷 108-112页

作者： Junichiro yamamoto megumi Masuda Kanae Hyodo Masahiro Iwasaki Division of Metabolism and Clinical Nutrition Kansai Electric Power Hospital Osaka Japan Laboratory of Physiology Faculty of Nutrition Kobe Gakuin University and Cooperative Research Center of Life Sciences Kobe Gakuin University Kobe Japan

Prevention of arterial thrombotic diseases has a high priority in developed countries. While an inappropriate diet is known to enhance the risk for acute thrombotic events, a regular diet with proven antithrombotic effects might be beneficial in the prevention of such diseases. The present study is part of a series of investigations aimed to assess the possible antithrombotic activity of various fruits and vegetables. Previously we demonstrated antithrombotic effect of specific potato varieties harvested in the spring. The present study aimed to test seven varieties of potatoes, which were harvested in the autumn. The in vitro Global Thrombosis Test (GTT) was used for selection, which was then followed by the He-Ne laser-induced in vivo thrombosis test in mice treated with potato filtrates orally. We have shown that all seven potato varieties showed some antithrombotic effect and the heat-resistant effect of four varieties was highly significant. Our present findings add further components to a diet containing fruits and vegetables with antithrombotic effect.

关键词： Cardiovascular Disease Stroke Antithrombotic Activity Global Thrombosis Test (GTT) Vegetables

2-Octyl-Cyanoacrylate Skin Adhesive Used as a Splinting Material in Auricular Surgery

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Modern Plastic Surgery 2016年第4期6卷 21-26页

作者： Naoto yamamoto Satoshi Yanagibayashi Ryuichi Yoshida Hiroyuki Ogi megumi Takikawa Akio Nishijima Eri Maruyama Department of Plastic and Reconstructive Surgery New Tokyo Hospital Chiba Japan Department of Plastic and Reconstructive Surgery Asakadai Central General Hospital Saitama Japan

In cases of auricular surgery, postoperative dressings are thought to be important for keeping auricular contour and in helping to prevent from dressing failures due to edema or subcutaneous hematoma, which may result in fibrous or cartilaginous proliferation. However, it is often difficult to achieve success with standard dressings because of the complicated shape of the auricle. We used 2-octyl-cyanoacrylate skin adhesive to dress the auricle after different types of auricular procedures (five cases of cryptotia, two of prominent ear, two of severe auricular laceration, two of skin grafting and one of flap repair of the partial auricle defect). The 2-octyl-cyanoacrlaate skin adhesive was applied to the suture line and the operated and peripheral areas for wider coverage. No dressing materials were placed over the surface. In all cases, the desired outcome was achieved, without subcutaneous hematoma, wound dehiscence, and wound infection. Contact dermatitis caused by the skin adhesive was not observed in any of the cases. Dressing and splinting after auricular surgery can be simply and successfully achieved using 2-octyl-cyanoacrylate skin adhesive. There is no need for more complicated dressings and post-surgical dressing changes, resulting in higher patient satisfaction.

关键词： Auricular Surgery Postoperative Dressings Splinting Material 2-Octyl-Cyanoacrylate Skin Adhesive

在膳食诱导的肥胖小鼠中,硝苯地平改善缺血引起的血管重建过程

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中华高血压杂志 2012年第6期20卷 598-598页

作者： Tetsutaro Kito Rei Shibata megumi Kondo Takashi yamamoto Hirohiko Suzuki Masakazu Ishii Toyoaki Murohara 罗冬梅叶鹏

肥胖是心血管疾病的危险因素之一，与血管功能受损相关。硝苯地平作为钙拮抗剂的一种，有许多除了降低血压以外的功能，如改善内皮细胞功能，降低氧化应激。

关键词：肥胖小鼠血管重建硝苯地平膳食诱导缺血内皮细胞功能心血管疾病危险因素