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Molecular methods for detection of pathogenic viruses of respiratory tract——A review

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Asian Pacific Journal of Tropical Biomedicine 2018年第5期8卷 237-244页

作者： md.shahidul Kabir Department of Microbiology Institutional Quality Assurance Cell Stamford University

Human respiratory system is harbored by a vast array of transient and normal microbial flora.A number of pathogenic viruses were diagnosed from samples in different occasions from mild to severe infections of respiratory *** methods were developed for detection of these viruses during last two *** acid amplification methods were introduced for rapid and accurate diagnosis of pathogenic *** detection systems were employed to identify a panel of pathogenic viruses,which requires specialized kits and instruments in some *** review summarizes different types of molecular approaches which were developed with time and applied for the detection of pathogenic viruses associated with infections of the respiratory system.

关键词： Respiratory system Virus Molecular method Detection

Sorbitol-based osmotic diarrhea:Possible causes and mechanism of prevention investigated in rats

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World Journal of Gastroenterology 2006年第47期12卷 7635-7641页

作者： md.shahidul islam Ei Sakaguchi Laboratory of Animal Nutri-tion Faculty of Agriculture Okayama University 1-1-1 Tsushima-naka Okayama City 700-8530 Japan

AIM: To study the possible causes of sorbitol (S)-based diarrhea and its mechanism of reduction by rice gruel (RG) in cecectomized rats. METHODS: S was dissolved either in distilled water or in RG (50 g/L) and ingested as a single oral dose (1.2 g/kg body mass, containing 0.5 g/L phenol red as a re-covery marker) by S (control) and S + RG groups (n = 7), respectively. This dose is over the laxative dose for hu-mans. Animals were sacrificed exactly 1 h after dose in-gestion, without any access to drinking water. The whole gastro-intestinal tract was divided into seven segments and sampled to analyze the S and marker remaining in its ***: Gastric-emptying and intestinal transit were comparatively slower in the S + RG group. Also, the S absorption index in the 3rd and last quarter of the small intestine (24.85 ± 18.88% vs 0.0 ± 0.0% and 39.09 ± 32.75% vs 0.0 ± 0.0%, respectively, P < 0.05) was sig-nificantly higher in the S + RG group than in the control group. The S absorption index and the intestinal fluid volume are inversely related to each other. CONCLUSION: The intestinal mal-absorption of S is the main reason for S-based osmotic diarrhea. Where RG en-hanced the absorption of S through passive diffusion, the degree of diarrhea was reduced in cecectomized rats.

关键词： Cecectomized rat Sorbitol Diarrhea Rice gruel Phenol red

Recent therapeutic targets for the prevention and management of diabetic complications

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世界糖尿病杂志:英文版(电子版) 2023年第9期14卷 1330-1333页

作者： md.shahidul islam Lu Cai Michael Horowitz Department of Biochemistry School of Life SciencesUniversity of KwaZulu-NatalDurban 4000KwaZulu-NatalSouth Africa Pediatric Research Institute University of LouisvilleLouisvilleKY 40202United States Department of Medicine University of AdelaideAdelaide 5005Australia

Diabetes and associated complications represent major global public health issues which are associated with impaired quality of life and premature *** some diabetic complications have decreased in the developed world,the majority are still prevalent,with an increasing trend in the developing *** used therapies are mainly‘glucocentric’,focusing on the optimization of glycemic control to prevent,delay or manage diabetes-associated complications-other common comorbidities,such as dyslipidemia and hypertension are often *** a number of novel therapeutic approaches have been reported recently,some of them have not received comparable attention in relation to either further studies or potential clinical *** editorial briefly discusses some recent therapeutic approaches to the prevention and management of diabetes and its associated complications,as well as potential directions for future research and development in this area.

关键词： Diabetic complications Oxidative stress Phytochemicals Zinc Silent information regulator 1 FOXO Micro RNA

Whole Leea macrophylla ethanolic extract normalizes kidney deposits and recovers renal impairments in an ethylene glycol-induced urolithiasis model of rats

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Asian Pacific Journal of Tropical Medicine 2012年第7期5卷 533-538页

作者： Abu Nasim Nizami md Atiar Rahman Nazim Uddin Ahmed md.shahidul islam Department of Biochemistry and Molecular Biology University of ChittagongChittagong-4331Bangladesh Department of Biochemistry School of BiochemistryGenetics and MicrobiologyUniversity of KwaZulu-NatalDurban 4000South Africa Bangladesh Council of Scientific and Industrial Research(BCSIR) Chittagong-4220Bangladesh

Objective:To investigate the antilithiatic effect of the whole Leea macrophylla(L macrophylla) Roxb(Leeaeeae) ethanol extract in ediylene glycol-induced urolithiasis model of ***: Forty two seven weeks old male wistar albino rats were randomly divided into two major groups namely:preventive(n=18) and therapeutic(n=24).Preventive group was further subdivided into 3 groups of 6 rats namely:preventive control(PC),preventive lithiatic control(PLC) and preventive lithiatic ***(PLLM).Similarly,therapeutic group was also subdivided into 4 groups of 6 rats namely:therapeutic control(TC),therapeutic lithiatic control(TLC).therapeutic lithitatic L macrophylla(TLLM) and therapeutic lithiatic cystone(TLCYS).The lithiasis was induced by 0.75%(v/v) ethylene glycol in the drinking water of all groups except the PC and TC *** urinary ionic parameters such as calcium,inorganic phosphate,oxalate,magnesium & creatinine and renal morphology were altered by ediylene glycol,which were partially recovered by 14 d preventive and almost fully recovered by 28 d therapeutic intervention trials with L macrophylla extract(500 mg/kg BW daily).Results:Significant difference on recovery was observed between preventive and dierapeutic interventional ***-urolithiatic effect of cystone was significantly(P<0.001) higher than extracts.L macrophylla extract was found nontoxic in the acute toxicity ***:The results of this study demonstrated very promising antiurolithiatic effect of *** extract with preventive and therapeutic treatments in this experimental condition.

关键词： Urolithiasis Ethylene glycol Leea macrophylla Urinary parameter Rats

Potential therapeutic targets for the prevention of diabetic nephropathy:Glycyrrhetinic acid

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World Journal of Diabetes 2023年第12期14卷 1717-1720页

作者： Lu Cai Michael Horowitz md.shahidul islam Pediatric Research Institute Department of PediatricsUniversity of LouisvilleLouisvilleKY 40202United States Department of Medicine University of AdelaideAdelaide 5005Australia Department of Biochemistry School of Life SciencesUniversity of KwaZulu-NatalDurban 4000KwaZulu-NatalSouth Africa

Uncontrolled hyperglycemia or poorly managed disease increases the propensityfor a number of diabetes-related complications targeting major organs includingthe heart, eyes, and kidney. Although the mechanisms by which diabetes inducescardiovascular diseases include oxidative stress and inflammation, when insulinresistance remains the key to the pathogenesis, as implicated in the two reviews inthis issue. This editorial mainly comments on the potential preventive applicationof glycyrrhetinic acid (or 18β-GA) in relation to diabetic nephropathy. The therapeuticor preventive effects of 18β-GA, as a hydrolytic product of glycy-rrhizicacid that is a component of licorice, have been appreciated in other disorders, buthave received much less attention in relation to diabetic complications. A study inthis issue has identified 18β-GA as a therapeutic for preventing diabeticnephropathy and provides evidence to support efficacy in cultured human renaltubule cells in vitro. Although it represents a pilot study, the observations supporta new therapeutic approach that warrants further ex-ploration.

关键词： Insulin resistance Diabetic cardiomyopathy Diabetic nephropathy Glycyrrhetinic acid Licorice Chinese herbal remedy

Vaccine efficacy and SARS-CoV-2 control in California and *** the session 2020e2026:A modeling study

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Infectious Disease Modelling 2022年第1期7卷 62-81页

作者： md Shahriar Mahmud md Kamrujjaman md Mashih Ibn Yasin Adan md Alamgir Hossain md Mizanur Rahman md.shahidul islam Muhammad Mohebujjaman md Mamun Molla Department of Computer Science and Engineering State University of BangladeshDhaka1205Bangladesh Department of Mathematics University of DhakaDhaka1000Bangladesh Department of Mathematics and Statistics University of CalgaryCalgaryABCanada Computational Biology Research Lab(CBRL) Department of PharmacyJagannath UniversityDhaka1100Bangladesh Hitotsubashi Institute for Advanced Study Hitotsubashi UniversityNaka KunitachiTokyo186-8601Japan Department of Mathematics and Physics Texas A&M International UniversityLaredoTX78041USA Department of Mathematics&Physics North South UniversityDhaka1229Bangladesh Center for Applied Scientific Computing(CASC) North South UniversityDhaka1229Bangladesh

Background:Besides maintaining health precautions,vaccination has been the only prevention from SARS-CoV-2,though no clinically proved 100%effective vaccine has been developed till *** this stage,to withhold the debris of this pandemic-experts need to know the impact of the vaccine efficacy rates,the threshold level of vaccine effectiveness and how long this pandemic may extent with vaccines that have different efficacy *** this article,a mathematical model study has been done on the importance of vaccination and vaccine efficiency rate during an ongoing ***:We simulated a five compartment mathematical model to analyze the pandemic scenario in both California,and whole *** considered four vaccines,Pfizer(95%),Moderna(94%),AstraZeneca(79%),and Johnson&Johnson(72%),which are being used rigorously to control the SARS-CoV-2 pandemic,in addition with two special cases:a vaccine with 100%efficacy rate and no vaccine under ***-CoV-2 related data of California,and *** used in this ***:Both the infection and death rates are very high in *** model suggests that the pandemic situation in California will be under control in the last quartile of the year 2023 if vaccination program is continued with the Pfizer *** this time,six waves may happen from the beginning of the immunization where the case fatality and recovery rates will be 1.697%and 98.30%,***,according to the considered model,this period might be extended to the mid of 2024 when vaccines with lower efficacy rates are *** the other hand,the daily cases and deaths in the *** be under control at the end of 2026 with multiple *** the number of susceptible people will fall down to none in the beginning of 2027,there is less chance to stop the vaccination program if vaccinated with a vaccine other than a 100%effective vaccine or Pfizer,and at that case vaccination program must run till the mid of *** to this s

关键词： Vaccine Model California Control measurement SARS-CoV-2

Morphological and Genetical Variability among Rhizoctonia solani Isolates Causing Sheath Blight Disease of Rice

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Rice science 2016年第1期23卷 42-50页

作者： Zakiah Rahman MONI md Ansar ALI md.shahidul ALAM md Asif RAHMAN md Rejwan BHUIYAN md Salim MIAN Khandakar md IFTEKHARUDDAULA md Abdul LATIF Mohammad Ashik Iqbal KHAN Plant Breeding Division Bangladesh Rice Research Institute Director Research Bangladesh Rice Research Institute Department of Botany Rajshahi University Department of Genetics and Plant Breeding Bangabandhu Sheikh Mujibur Rahman Agricultural University Plant Pathology Division Bangladesh Rice Research Institute

Eighteen isolates of Rhizoctonia solani collected from infected rice plants in four different locations of Bangladesh were studied by using morphological characters and molecular markers. Anastomosis study with a reference isolate confirmed that all the isolates belonged to R. solani. Significant variation was observed in sclerotial size, shape and distribution. Un-weighted pair group method with arithmetic mean dendrogram constructed based on the Gower＇s general similarity coefficient showed that these isolates were grouped into four clusters at the 0.68 similarity coefficent according to morphological characters. Cluster I was a major cluster consisting of 13 isolates, while clusters Ⅱ to Ⅳ consisted of 1 or 2 isolates. Analyses by variable number of tandem repeat and amplified fragment length polymorphism markers showed that the isolates were grouped into five and three clusters at a similarity coefficient of 0.64 and 0.69, respectively. Although most of the variability was found between isolates from different regions as expected, significant variation was observed within the isolates collected from similar agro-ecological regions. Our results suggest the presence of different races of R. solani within the same local geographic regions.

关键词： rice Rhizoctonia solani fungal variability molecular marker morphological character

Meeting report of the chief editorial board meeting for World Journal of Diabetes 2021

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World Journal of Diabetes 2021年第12期12卷 1969-1978页

作者： Jin-Lei Wang Jia-Ping Yan Xiang Li md.shahidul islam Jian-Bo Xiao Lu Cai Na Ma Li Ma Lian-Sheng Ma Baishideng Publishing Group Inc PleasantonCA 94566United States Department of Biochemistry School of Life SciencesUniversity of KwaZulu-Natal(Westville Campus)Durban 4000South Africa Faculty of Sciences Department of Analytical and Food ChemistryNutrition and Bromatology GroupUniversidade de VigoOurense 32004Spain Pediatric Research Institute Department of PediatricsUniversity of LouisvilleLouisvilleKY 40202United States

The 2021 online editorial board meeting of the World Journal of Diabetes(WJD)was held on November 9,***-Lei Wang,General Manager on behalf of the Baishideng Publishing Group,and Professor islam,one of the Editors-in-Chiefs(EiCs)of the WJD,organized the *** EiCs and 18 Baishideng Publishing Group staff attended the *** meeting goal was to brief the EiCs on the journal’s performance,discuss the issues of concern of the EiCs,and gather ideas for the journal’s development in *** of November 8,the WJD had received 287 manuscripts since the year’s start,among which 122 met the criteria for *** numbers represent an increase of 117.4%for submissions and 110.3%for publications compared to those in ***,how to effectively control the academic quality of manuscripts and attract highquality original article submissions remain a *** EiCs provided feedback and suggestions centered on three topics:(1)Who should and how to control the academic quality of the manuscripts;(2)How the EiCs perform their responsibilities;and(3)The distinctive and shared responsibilities of the publisher and the EiCs.

关键词： World Journal of Diabetes Baishideng Editorial board meeting Journal development Bibliometrics Scientific literature

Effect of Plant Extracts on Seed Borne Fungi of Jute

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American Journal of Plant Sciences 2018年第13期9卷 2580-2592页

作者： md. Abdul Ahad md. shahidul islam Nur Fatema Nupur Department of Plant Pathology Bangladesh Agricultural University Mymensingh Bangladesh Department of Plant Pathology Yunnan Agricultural University Kunming China Department of Fisheries Management Bangladesh Agricultural University Mymensingh Bangladesh

The aim of this study was to determine the prevalence of seed-borne fungal pathogens of jute and their control with plant extract. The experiment was carried out in seed pathology centre, Bangladesh Agricultural University to obtain relevant information about the health of jute seeds and find out the efficacy of extracts as seed-treater. Two varieties viz., Deshipat (Corchorus capsularis) and Tossapat (Corchorus olitorius) were selected for this study and jute seeds were collected from two different locations of Bangladesh. The major predominant identified fungi were Colletotrichum corchori, Macrophomina phaseolina, Fusarium spp., and Botryodiplodia theobromae. Least seed-borne fungi were found in the variety of Tossapat. Low germination of seeds was found related to high prevalence of seed-borne fungal infections. Highest seed-borne infection was obtained in the variety of Deshipat. In this study, we evaluated some major plant extracts such as garlic clove (Allium sativum), neem leaves (Azadirachta indica), allamanda leaves (Allamanda cathartica L.) and marigold leaves (Tagetes spp.) for observing the action of these plant extracts on seed borne fungi of Jute. The study revealed that the extract of Garlic (Allium sativum), and Biskatali (Polygonum hydropiper) increased the germination of seeds and garlic?(Allium sativum) extract was also found to be most effective in controlling the seed-borne infection among all the treatments. Although the inhibiting capacity of the extracts of Allamanda (Allamanda cathartica L.) and Biskatali (Polygonum hydropiper) was found to be similar and Biskatali extract was found to have the second position among all the treated plant extracts in inhibiting the growth of isolated fungal pathogens.

关键词： Botanical Treatment Medicinal Plant Bio-Control Seed Jute