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Aeromonas veronii isolated from climbing perch(Anabas testudineus)suffering from epizootic ulcerative syndrome(EUS)

Aquaculture and Fisheries 2023年第3期8卷 288-295页

作者： Rakib Ehsan Ashikur Rahman Sulav Indra Paul md.Ali Arman Ador md.shameul haque Tasmina Akter md.Mahbubur Rahman Institute of Biotechnology and Genetic Engineering Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur1706Bangladesh Department of Fisheries Management Faculty of FisheriesBangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur1706Bangladesh

Motile Aeromonads are responsible for various diseases in fishes that cause significant economic losses in *** *** also frequently isolated from fish suffering from Epizootic Ulcerative Syndrome(EUS).The present study was conducted to isolate and identify *** from Vietnam climbing perch(Anabas testudineus)suffering from EUS,evaluate the pathogenicity of the isolates,and find out antibiogram profile and susceptibility of the isolates to medicinal plant *** climbing perch(***)suffering from EUS was collected from fish farms located at Savar,Dhaka and bacteria were isolated from the external ulcerative lesion,and kidney and liver of the infected fish on nutrient agar *** phenotypic identification of the bacterial isolates was performed following morphological,physiological and biochemical characterization *** 16S rRNA gene sequencing was accomplished for further molecular identification of five selected *** evaluate the pathogenicity of the isolates in vivo artificial infection challenge test was conducted through immersion *** in vivo pathogenicity test was done at three different temperatures(18◦C,28◦C,and 37◦C)to examine the effects of temperature on the pathogenicity of the *** commercial antibiotics were used to find out the antibiogram profile of the ***,susceptibility of the isolates was screened against 19 medicinal plants extracts.A total of 38 out of 46 isolates isolated from Vietnam climbing perch(***)suffering from EUS were phenotypically identified as Aeromonas *** randomly selected isolates were further confirmed as *** by analyzing the 16S rRNA gene sequence *** pathogenicity of the isolates was found moderate to high(41%-80%mortality)in the artificially challenged *** highest and lowest mortality was obtained in the fish challenged with the isolates BFKA33 and BFKA12,*** isolates were found resis

关键词： Aeromonas veronii Anabas testudineus,Epizootic ulcerative syndrome Antibiogram Medicinal plant extracts

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