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Ferulic Acid in Paper Mulberry and Its Wastewater from Pulps Production

Journal of Chemistry and Chemical Engineering 2010年第7期4卷 58-62页

作者： mayuree kanlayavattanakul nattaya lourith School of Cosmetic Science Mae Fah Luang University Chiang Rai 57100 Thailand

Wastewater from paper mulberry （Broussonetia papyrifera） pulps production was subjected to biological activities evaluations and phenolics characterization. The EtOAc extract was found to be the best antioxidant （IC50 = 0.4408 mg/mL） and tyrosinase inhibitor （IC50 = 1.6340 mg/mL） comparing to vitamin C （1C50 = 0.0011 mg/mL） and kojic acid （IC50 = 0.0244 mg/mL）, respectively, and the highest ferulic acid content （4.450 ±0.0077 ppm）. This fraction was further column chromatographed and antioxidant activity was archived （IC50 = 0.075 mg/mL） as well as tyrosinase inhibitory effect （IC50 = 0.042 mg/mL） in addition to ferulic acid （45.310 ± 0.0115 ppm）. Ferulic acid in paper mulberry was compared. Reflux with H2O gave the highest ferulic acid content （37.848 ± 1.2470 ppm） followed by a mixture of MeOH and H2O （2.002 ± 0.0370 ppm） and MeOH （1.185 ± 0.0320 ppm）, respectively. The reflux H2O was partitioned with EtOAc that extracted most of ferulic acid （256.678 ± 0.3760 ppm） and left less in the aqueous layer （9.408 ± 0.2820 ppm）.

关键词： Paper mulberry broussonetia papyrifera wastewater tyrosinase inhibitor DPPH-radical scavenging activity.

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Preparation and characterization of nanoparticles from quaternized cyclodextrin-grafted chitosan associated with hyaluronic acid for cosmetics

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Asian Journal of Pharmaceutical Sciences 2018年第5期13卷 498-504页

作者： Sakhiran Sakulwech nattaya lourith Uracha Ruktanonchai mayuree kanlayavattanakul School of Cosmetic Science Mae Fah Luang University Phytocosmetics and Cosmeceuticals Research Group Mae Fah Luang University National Nanotechnology Center National Science and Technology Development Agency

Hyaluronic acid(HA, 20–50 kDa) is a hydrophilic macromolecule with anti-wrinkle effects and moisturizing properties. However, its high molecular weight prevents it from penetrating into the deeper layers of the skin and, thus, limits its benefits to topical effects. Thus, the objective of this study is to prepare nanoparticles of quaternized cyclodextrin-grafted chitosan(QCD-g-CS) associated with HA in different molar ratios of QCD-g-CS and HA. The conjugation of the carboxylic moieties of HA and the amides of QCD-g-CS was confirmed by Fourier-transform infrared spectroscopy. Thus, the system was optimized to create nanoparticles with a small size(235.63 ± 21.89 nm), narrow polydispersity index(0.13 ± 0.02), and zeta potential of 16.07 ± 0.65 m V. The association efficiency and loading efficiency were determined by ultra-performance liquid chromatography as 86.77 ± 0.69% and 10.85 ± 0.09%, respectively. The spherical morphology of the obtained nanoparticles was confirmed by transmission electron microscopy. Moreover, the in-vitro hydrating ability was significantly higher( P < 0.001) than that of bulk HA(3.29 ± 0.41 and 1.71 ± 0.05 g water/g sample, respectively). The safety of these nanoparticles at concentrations in the range of 0.01–0.10 mg/ml was confirmed via tests on human skin fibroblasts. Together, these results demonstrate that the developed nanoparticles are promising for future applications in cosmetics.

关键词： Hyaluronic acid QCD-g-CS Polymeric nanoparticles Ionic gelation Delivery system

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Dendrobium orchid polysaccharide extract: Preparation,characterization and in vivo skin hydrating efficacy

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Chinese Herbal Medicines 2019年第4期11卷 400-405页

作者： mayuree kanlayavattanakul Tamonpatch Pawakongbun nattaya lourith School of Cosmetic Science Mae Fah Luang UniversityChiang Rai 57100Thailand Phytocosmetics and Cosmeceuticals Research Group Mae Fah Luang UniversityChiang Rai 57100Thailand

Objective:Dendrobium spp.,the major globally commercializing tropical orchid,has been continuously used in Chinese ***,preparation and standardization of this therapeutic orchid including its clinical evidence for topical application are sparely to be ***:Maceration of the white orchid(Dendrobium *** Sanan)in water was varied on the extraction *** extraction yields and total polysaccharide content of the extracts were *** best extract condition was selected,standardized,and evaluated on safety and skin hydrating efficacy in human ***:The extraction for 3 h gave the extract with polysaccharide content that significantly(P<0.001)greater than the other extraction times with the following specification:yield[(35.60±2.51)%],moisture content[(7.65±0.21)%],total polysaccharide,sugar,reducing sugar and ash content[(72.95±2.37)%,(34.38±0.00)%,(16.28±0.00)%,and(0.12±0.00)%],p H(4.42±0.01),viscosity[(15.07±0.12)cps]swelling and water absorption capacities[(10.00±0.00)%,(1.19±0.24)g/g].This antioxidative polysaccharide caused no skin irritation with a better skin hydrating efficacy than the untreated skin and the benchmark as examined in 22 Thai ***:White Dendrobium is ready to be supplied as a specialty ingredient with a safe and efficient profile for skin dryness *** innovative application of the identified traditional Chinese medicinal herb flows in the main stream of the consumers’preferences and demand upon natural derived products.

关键词： clinical study moisturizer orchid phytopharmaceuticals polysaccharide skin hydrating

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