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Microwave Assisted Extraction,Antioxidant Potential and Chromatographic Studies of Some Rasayana Drugs

Chinese Journal of Integrative Medicine 2015年第7期21卷 523-529页

作者： Ashish Mishra Shilpi Mishra Shilpi Bhargav CS.Bhargava mayank thakur Advance Institute of Biotech&Paramedical Sciences Institute for Laboratory Medicine Clinical Chemistry and Pathobiochemistry Charite University of MedicineCampus Benjamin Franklin

Objective： To study and compare the conventional extraction procedure with microwave assisted extraction （MAE） for some Ayurvedic Rasayana drugs and to evaluate their antioxidant potential and carry out the characterization of extracts by thin layer chromatography. Methods： Three Ayurvedic rasayana plants Allium sativum Linn., Bombax ceiba Linn. and Inula racemosa Hook. were evaluated for an improved MAE methodology by determining the effects of grinding degree, extraction solvent, effect of dielectric constant and duration of time on the extractive value. Antioxidant potential of all three drugs was evaluated with 2,2-diphenyl-l-picrylhydrazyl （DPPH） radical scavenging activity and reducing power was determined by using Gallic acid as standard. Further thin layer chromatographic （TLC） analysis was performed on pre-activated Silica Gel G plates and Rf value were compared with those reported for the important biomarkers. Results： The total extractive value for Allium sativum Linn. was 36.95% （w/w） and 49.95% （w/w） for ethanol extraction respectively. In case of Bombax ceiba Linn. the yield of aqueous extract by MAE was 50% （w/w） compared to 42% （w/w） in ethanol （50% v/v）. Percent yield of Inula racernosa Hook. in aqueous extract was found to be 27.55% （w/w） which was better than ethanol extract （50%） where the yield was 25.95% （w/w）. Upon antioxidant activity evaluation A. safivum extract showed an absorbance of 0.980 ± 0.92 at concentration of 500 μg with maximum reducing capacity. This was followed by B. ceiba Linn. 0.825 ± 0.98 and I. racemosa Hook. with 0.799 ± 2.01 at a concentration of 500 μ g. TLC based standardization of A. sativum Linn. extract shows single spot with Rf value of 0.38, B. ceiba Linn. extract shows Rf values were 0.23, 0.58, 0.77, 0.92 and I. racemosa Hook. extract spot had a Rf value of 0.72. Conclusions： A significant improvement in extractive values was observed as a factor of time and other advantages by using MAE technology. All three dru

关键词： antioxidant potential Ayurvedic Rasayana Allinm sativum Linn. Bombax ceiba Inula racemosa,microwave assisted extraction

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Effect of Chlorophytum Borivilianum Santapau and Fernandes on Sexual Dysfunction in Hyperglycemic Male Rats

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Chinese Journal of Integrative Medicine 2009年第6期15卷 448-453页

作者： mayank thakur Shilpi Bhargava Werner Praznik Renate Loeppert Vinod K Dixit Department of Pharmaceutical Sciences Dr. Hari Singh Gour University Department of Chemistry University of Natural and Applied Life Sciences Dr.Hari Singh Gour University

Objective:To investigate the effects of Chlorophytum borivilianum(CB) on sexual dysfunction, loss of body weight,and lack of libido in hyperglycemic rats induced with streptozotocin or ***: Wistar strain male albino rats were divided into five groups of six animals each:the control group(2% polyvinylpyrollidone solution),the streptozotocin control group(50 mg/kg),the alloxan control group(100 mg/kg), the streptozotocin + CB treated group(200 mg/kg),and the alloxan + CB treated group(200 mg/kg).O...

关键词： ayurvedic rasayana Chlorophytum borivilianum diabetes induced sexual dysfunction Safed Musli

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仙茅:对健康有众多益处的黑金(英文)

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中西医结合学报 2010年第7期8卷 613-623页

作者： Nagendra Singh Chauhan Vikas Sharma mayank thakur Vinod Kumar Dixit Department of Pharmaceutical Sciences Dr.Hari Singh Gour University

仙茅(石蒜科)是一种濒临灭绝的多年生草本植物,是印度本土药,作为强有力的适应原和春药在印度传统药物中占有重要位置。它也是多种印度传统药物处方的重要组成部分,被认为具有促进性欲、刺激免疫、保护肝脏、抗氧化、抗癌和治疗糖尿病... 详细信息

仙茅(石蒜科)是一种濒临灭绝的多年生草本植物,是印度本土药,作为强有力的适应原和春药在印度传统药物中占有重要位置。它也是多种印度传统药物处方的重要组成部分,被认为具有促进性欲、刺激免疫、保护肝脏、抗氧化、抗癌和治疗糖尿病等作用。已有报道,从仙茅中提取出多种化学成分,如植物黏胶,酚苷、皂苷和脂肪族化合物等。仙茅也是中医和日本汉方医学中各种草药处方的重要成分。本文对仙茅的多种生物活性和其不同化学成分的作用进行了综述。

关键词：仙茅催欲药医学,印度传统提取物

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南欧派利吞草石油醚提取物对雄性大鼠性行为的影响(英文)

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中西医结合学报 2010年第8期8卷 767-773页

作者： Vikas Sharma mayank thakur Nagendra S.Chauhan Vinod Kumar Dixit Department of Pharmaceutical Sciences Dr.Hari Singh Gour Vishwavidyalaya

目的:检测南欧派利吞草石油醚提取物(petroleum ether extract,PEE)对雄性大鼠性行为的影响。方法:制备南欧派利吞草PEE。32只雄性Wistar大鼠分成对照组、睾酮组、低剂量PEE组和高剂量PEE组,每组8只。对照组大鼠灌胃花生油(溶剂对照),... 详细信息

目的:检测南欧派利吞草石油醚提取物(petroleum ether extract,PEE)对雄性大鼠性行为的影响。方法:制备南欧派利吞草PEE。32只雄性Wistar大鼠分成对照组、睾酮组、低剂量PEE组和高剂量PEE组,每组8只。对照组大鼠灌胃花生油(溶剂对照),睾酮组大鼠肌肉注射0.5mg/kg睾酮,每周2次,低剂量和高剂量PEE组大鼠分别灌胃50和100mg/kgPEE,治疗持续28d。于治疗前,治疗第15天、第28天和治疗后第7天、第15天,检测大鼠体质量,治疗结束后,取4只大鼠性器官(睾丸、附睾、精囊、前列腺)称取质量,其余4只大鼠用于检测大鼠的性取向行为和性功能。结果:与对照组大鼠比较,PEE治疗28d可明显增加雄性大鼠的体质量和性器官质量,雄性大鼠易于接受雌性大鼠和对雌性大鼠趋向性增加,雄性大鼠性能力增加,表现为舔、嗅雌性大鼠肛门和生殖器,阴茎勃起指数增加,交配潜伏期缩短,交配频率增加。在PEE治疗结束后7和15d仍观察到以上行为和性指标的变化。结论:与睾酮不同的是,PEE治疗结束后7和15d仍可见明显治疗效果,提示南欧派利吞草能提高大鼠性潜能,并有延迟效应。

关键词：南欧派利吞草催欲药植物提取物石油醚提取物性行为,动物烷基酰胺大鼠

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Comparison of rare earths doping(neodymium and samarium) on structural and magnetic properties of Ni-Zn-Bi nanoferrites

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Journal of Rare Earths 2024年第3期42卷 534-542,I0004页

作者： Shilpa Taneja Preeti thakur Dinesh Kumar Yassine Slimani Atul thakur NanoLatticeX Amity University HaryanaGururam122413HaryanaIndia Nanotechnology Wing Innovative Science Research SocietyShimla177002India Centre for Nanotechnology Amity University HaryanaGururam122413HaryanaIndia Department of Biophysics Institute for Research and Medical Consultations(IRMC)Imam Abdulrahman Bin Faisal UniversityP.O.Box 1982Dammam31441Saudi Arabia School of Electronics and Information Engineering Nanjing University of Information Science&TechnologyNanjing210044China

Excellent magnetic properties in ferrites are required for high-frequency applications and for wastewater treatment. Thus, the present study shows the comparison of magnetic and structural properties of Nd and Sm substituted Ni-Zn-Bi ferrites with the series Ni_(0.5)Zn_(0.5)Bi_(0.04)Nd_(x)Fe_(1.96-x)O_4(with step size 0.002)and Ni_(0.5)Zn_(0.5)Bi_(0.04)Sm_(x)Fe_(1.96-x)O_4(with step size 0.02) prepared using citrate precursor method. The impact of the substitution of rare earth ions(Nd and Sm) on magnetic properties of the synthesized samples is observed using a vibrating sample magnetometer(VSM). The saturation magnetization values enhance considerably from 52 to 58 emu/g for Nd^(3+)ions and 39 to 57 emu/g for Sm^(3+) ions, thus, making these materials magnetically hard. Further, the higher value of coercivity is also observed ranging from133 to 167 Oe for Nd^(3+)ions and 81 to 155 Oe for Sm^(3+) ions. The shape of hysteresis loops indicates a super paramagnetic and ferromagnetic behavior in the obtained samples. The squareness ratio value is<0.5, suggesting the uniaxial anisotropy of particles and hence, these ferrites are suitable for microwaveabsorbing and in permanent magnetic materials. The X-ray diffraction(XRD) pattern shows the formation of pure cubic crystallites, where, lattice parameters range from 0.840 to 0.839 nm and from 0.838to 0.839 nm for Nd^(3+)and Sm^(3+) ions substitution, respectively. The crystallite size ranges between 28.63to 29.89 nm and 18.33 to 26.23 nm, for substitution of Nd^(3+)and Sm^(3+) ions, respectively. Field emission scanning electron microscopy(FESEM) shows the formation of homogeneous grains, whereas, energy dispersive spectrometer(EDS) counts describe the purity of the samples. The Sm concentration x = 0.10has the maximum surface area with value of 42.6 m^(2)/g which proves to be having good data storage application due to high surface area. The zero-field cooled(ZFC) and field cooled(FC) data show that Nddoped Ni-Zn-Bi nano

关键词： Citrate precursor X-ray diffraction Vibrating sample magnetometer Coercivity Field emission scanning electron microscopy Rare earths

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Identification and characterization of integron mediated antibiotic resistance in pentachlorophenol degrading bacterium isolated from the chemostat

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Journal of Environmental Sciences 2009年第6期21卷 858-864页

作者： SHARMA Ashwani thakur Indu Shekhar Environmental Biotechnology Laboratory School of Environmental SciencesJawaharlal Nehru UniversityNew Mehrauli Road

A bacterial consortium was developed by continuous enrichment of microbial population isolated from sediment core of pulp and paper mill effluent in mineral salts medium（MSM） supplemented with pentachlorophenol（PCP） as sole source of carbon and energy in the *** consortia contained three bacterial *** were identified as Escherichia coli,Pseudomonas aeruginosa and Acinetobacter *** 16S rRNA gene sequence *** *** degraded PCP through the formation of tetrachloro-p-hydroquinone（TecH）,2-chloro-1,4-benzenediol and products of ortho ring cleavage detected by gas chromatograph/mass spectrometer（GC-MS）.Out of the three acclimated PCP degrading bacterial strains only one strain,Acinetobacter *** the presence of integron gene cassette as a marker of its stability and antibiotic *** strain possessed a 4.17 kb amplicon with 22 ORF＇*** plasmid isolated from the Acinetobacter *** subjected to shotgun cloning through restriction digestion by BamHI,HindIII and SalI,ligated to pUC19 vector and transformed into *** XLBlue1α,and finally selected on MSM containing PCP as sole source of carbon and energy with ampicillin as antibiotic *** sequence analysis of recombinant clones indicated homology with integron gene cassette and multiple antibiotic resistance genes.

关键词： Acinetobacter sp. antibiotic resistance gene chemostat integron gene cassette pentachlorophenol recombinant clone

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Towards POI-based large-scale land use modeling: spatial scale, semantic granularity, and geographic context

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International Journal of Digital Earth 2023年第1期16卷 430-445页

作者： Junchuan Fan Gautam thakur Geospatial Science and Human Security Division Oak Ridge National LaboratoryOak RidgeTNUSA

The combination of spatial distribution,semantic characteristics,and sometimes temporal dynamics of POIs inside a geographic region can capture its unique land use *** previous studies on POI-based land use modeling research focused on one geographic region and select one spatial scale and semantic granularity for land use *** is a lack of understanding on the impact of spatial scale,semantic granularity,and geographic context on POI-based land use modeling,particularly large-scale land use *** this study,we developed a scalable POI-based land use modeling framework and examined the impact of these three factors on POI-based land use characterization using data from three geographic *** developed a unified semantic representation framework for POI semantics that can help fuse heterogeneous POI data ***,by combining POIs with a neural network language model,we developed a spatially explicit approach to learn the embedding representation of POIs and *** trained multiple supervised classifiers using AOI embeddings as input features to predict AOI land use at different semantic *** classification performance of different land use classes was analyzed and compared across three geographic regions to identify the semantic representativeness of POI-based AOI embedding and the impact of geographic context.

关键词： Land use POI geospatial semantic deep learning semantic granularity spatial scale

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Electroless nickel fabrication on surface modified magnesium substrates

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Defence Technology（防务技术） 2019年第4期15卷 636-644页

作者： Ayushi thakur Swaroop Gharde Balasubramanian Kandasubramanian Department of Metallurgical and Materials Engineering National Institute of Technology Hazratbal Srinagar Jammu & Kashmir 190006 India Department of Metallurgical & Materials Engineering DIAT (DU) Ministry of Defence Girinagar Pune 411025 Maharashtra India

In recent years,magnesium(Mg)has evolved as a salient material,in affiliation with electroless nickel(Ni)coating,which have found applications in automobiles,aerospace and confederate fields attributing to its excellent inherent weight sensitive ***,being acknowledged for its remarkable auxiliary properties like flexible machining,appreciable weight sensitivity and ability to be patently diecast into mesh constructs,magnesium is prejudiced by aeronautical standards predominantly for its inferior corrosion resistance *** this sense,electroless nickel plating on magnesium and its alloys has been suggested to extricate it from corrosion problem and make it more competitive in industrial and defence *** fixation of metal ions onto respective substrates accrues and alters their mechanical,electrochemical and tribological properties,destitute of any electric current *** proficiently identified technique is prosecuted with the assistance of a series of sequenced operations involving a prior pretreatment,which corresponds to the chemical cleaning of the substrate surface;electroless coating;and a later activation process which is a mild etching of the electroless coated *** susceptibility of magnesium to this methodology has advanced and propagated its exercise and applicability in aircraft,satellites and allied aeronautical ***,researchers have proposed various eco-friendly and modified duplex and composite coatings which have transmuted properties of these appendages by tailoring alloy compositions and reagents *** review article systematically colligates various considerations and evaluations on electroless nickel applications of magnesium and its alloys and explicates how it anchors its practice in the respective ***,a comprehensive analysis is devised based on the pre-existing treatment methods for accomplishing the same.

关键词： Electroless nickel plating Magnesium Magnesium alloys Corrosion-resistance Micro arc oxidation(MAO)

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Effect of pulse slippage on density transition-based resonant third-harmonic generation of short-pulse laser in plasma

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Frontiers of physics 2016年第4期11卷 163-170页

作者： Vishal thakur Niti Kant Department of Physics Lovely Professional University Phagwara-144411 Punjab India

The resonant third-harmonic generation of a tion was investigated. Because of self-focusing self-focusing laser in plasma with a density transi- of the fundamental laser pulse, a transverse intensity gradient was created, which generated a plasma wave at the fundamental wave frequency. Phase matching was satisfied by using a Wiggler magnetic field, which provided additional angular too- mentum to the third-harmonic photon to make the process resonant. An enhancement was observed in the resonant third-harmonic generation of an intense short-pulse laser in plasma embedded with a magnetic Wiggler with a density transition. A plasma density ramp played an important role in the self-focusing, enhancing the third-harmonic generation in plasma. We also examined the ef- fect of the Wiggler magnetic field on the pulse slippage of the third-harmonic pulse in plasma. The pulse slippage was due to the group-velocity mismatch between the fundamental and third-harmonic pulses.

关键词： short pulse laser pulse slippage third harmonic generation plasma

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Influence of gaseous and particulate species on neutralization processes of polar aerosol and snow——A case study from Ny-?lesund

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Journal of Environmental Sciences 2019年第2期31卷 12-25页

作者： Roseline C.thakur Meloth Thamban ESSO-National Centre for Antarctic and Ocean Research Institute of Atmospheric and Earth System Research University of Helsinki

The inter-conversion of nitrogen and sulfur species between the gas and particulate phases and their interaction with alkaline species influences the acidity of the aerosols and surface snow. To better understand these processes, a short field campaign was undertaken in Ny-?lesund,Svalbard, during 13th April 2012 to 24th April 2012. Air measurements were carried out through a particulate sampler equipped with denuders and filter packs for simultaneous collection of trace gases(HNO_3, NO_2, SO_2 and reactive nitrogen compounds) and aerosols, with daily collection of snow samples. Ionic composition of the samples was analyzed using ion chromatography technique. The results suggested that nitrate-rich aerosols are formed when PAN(peroxy acetyl nitrate) disassociates to form NO_2 and HNO_3 which further hydrolyzes to form pNO_3^-(particulate nitrate). This resulted in a high contribution of pNO_3^-(62%) to the total nitrogen budget over the study area. The acidity of the aerosols and snow evaluated through cation/anion ratio(C/A)indicated alkaline conditions with C/A > 2. The bicarbonates/carbonates of Mg^(2+) played an important role in neutralization processes of surface snow while the role of NH_3 was dominant in aerosol neutralization processes. Such neutralization processes can increase the aerosol hygroscopicity causing warming. Chloride depletion in the snow was significant as compared to the aerosols, indicating two important processes, scavenging of coarse sea salt by the snow and gaseous adsorption of SO_2 on the snow surface. However, a more systematic and long term study is required for a better understanding of the neutralization processes and chemical interconversions.

关键词： Particulate Aerosol Scavenging Neutralization Arctic

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