Identification and characterization of integron mediated antibiotic resistance in pentachlorophenol degrading bacterium isolated from the chemostat

作     者：SHARMA Ashwani THAKUR Indu Shekhar 

作者机构：Environmental Biotechnology LaboratorySchool of Environmental SciencesJawaharlal Nehru UniversityNew Mehrauli Road 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2009年第21卷第6期

页      面：858-864页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 0903[农学-农业资源与环境] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 0713[理学-生态学] 

基　　金：We are indebted to Department of Science and Technology  Ministry of Science and Technology  Government of India  New Delhi for providing funds in the form of research projects 

主　　题：Acinetobacter sp. antibiotic resistance gene chemostat integron gene cassette pentachlorophenol recombinant clone 

摘      要：A bacterial consortium was developed by continuous enrichment of microbial population isolated from sediment core of pulp and paper mill effluent in mineral salts medium（MSM） supplemented with pentachlorophenol（PCP） as sole source of carbon and energy in the *** consortia contained three bacterial *** were identified as Escherichia coli,Pseudomonas aeruginosa and Acinetobacter *** 16S rRNA gene sequence *** *** degraded PCP through the formation of tetrachloro-p-hydroquinone（TecH）,2-chloro-1,4-benzenediol and products of ortho ring cleavage detected by gas chromatograph/mass spectrometer（GC-MS）.Out of the three acclimated PCP degrading bacterial strains only one strain,Acinetobacter *** the presence of integron gene cassette as a marker of its stability and antibiotic *** strain possessed a 4.17 kb amplicon with 22 ORF＇*** plasmid isolated from the Acinetobacter *** subjected to shotgun cloning through restriction digestion by BamHI,HindIII and SalI,ligated to pUC19 vector and transformed into *** XLBlue1α,and finally selected on MSM containing PCP as sole source of carbon and energy with ampicillin as antibiotic *** sequence analysis of recombinant clones indicated homology with integron gene cassette and multiple antibiotic resistance genes.