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基于双层双能CT衍生的肺灌注图鉴别急性肺栓塞和慢性血栓栓塞性肺动脉高压

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国际医学放射学杂志 2024年第4期 495-495页

作者： R.j.Gertz F.gerhardt M.Pienn S.Lennartz j.R.Kr?ger L.Caldeira 刘晓彤

摘要目的旨在评估双层双能CT(dlDECT)衍生的肺灌注影像在鉴别急性肺栓塞（PE）和慢性血栓栓塞性肺动脉高压（CTEPH）中的应用。方法回顾性纳入在dlDECT上行CT

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Oxygen-deficient titanium dioxide supported cobalt nano-dots as efficient cathode material for lithium-sulfur batteries

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j.urnal of Energy Chemistry 2020年第9期29卷 390-397,I0012页

作者： You Li Xiao Zhang Guihua Liu Ashton gerhardt Katelyn Evans Aizhong j.a Zisheng Zhang Hebei Provincial Key Laboratory of Green Chemical Technology and High Efficient Energy Saving Tianjin Key Laboratory of Chemical Process SafetySchool of Chemical Engineering and TechnologyHebei University of TechnologyTianjin 300130China Department of Chemical and Biological Engineering University of OttawaOttawaOntario K1N 6N5Canada

Lithium sulfur(Li-S)batteries demonstrate great promise for efficient energy storage systems once the lithium polysulfide(LPS)shuttling and sluggish redox kinetics can be well ***,we developed a sea urchin-structured oxygen-deficient titanium dioxide semiconductor anchored with cobalt nano-dots(Co@TiO2-x)as a high-performance multifunctional sulfur host material for Li-S *** sea urchin-structured Co@TiO2-x offers strong structural stability and strengthened chemical interaction towards ***,the incorporation of Co nano-dots into TiO2 leads to increased oxygen vacancies,which augments the electrical conduction and benefits LPS conversion acceleration as *** a result,the oxygen vacancy-rich Co@TiO2-x composite exhibits excellent conductivity,strong LPS confinement and promoted sulfur electrochemical kinetics,rendering enhanced LPS shuttling inhibition and rapid redox *** to these features,the Co@TiO2-x/S cathode exhibits a discharge capacity of 803 mAh g-1at 1 C and a good cyclic stability upon 500 cycles with a low capacity fading rate of 0.07%per *** synergistic design of conductive multifunctional LPS barrier is also promising to enlighten the material engineering in other energy storage applications.

关键词： Titanium dioxide Oxygen vacancy Cobalt nano-dots Lithium-sulfur battery Conversion kinetics

A 96-wells fluidic system for high-throughput screenings under laminar high wall shear stress conditions

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Microsystems & Nanoengineering 2023年第5期9卷 203-217页

作者： Catarina Gonçalves Fonseca Vânia Silvério David Barata Wolfgang Giese Holger gerhardt Susana Cardoso Claudio Areias Franco Instituto de Medicina Molecular j.ão Lobo Antunes Faculdade de MedicinaUniversidade de LisboaLisboaPortugal INESC Microsistemas and Nanotecnologias INESC-MNLisboaPortugal Department of Physics Instituto Superior TécnicoUniversidade de LisboaLisboaPortugal Max Delbrück Center for Molecular Medicine BerlinGermany DZHK(German Centre for Cardiovascular Research) Partner Site BerlinBerlinGermany Charité–Universitätsmedizin Berlin BerlinGermany Universidade Católica Portuguesa Católica Medical SchoolCatólica Biomedical Research CentreLisbonPortugal

The ability of endothelial cells to respond to blood flow is fundamental for the correct formation and maintenance of a functional and hierarchically organized vascular *** flow responses,in particular related to high flow conditions,have been associated with atherosclerosis,stroke,arteriovenous malformations,and neurodegenerative ***,the molecular mechanisms involved in high flow response are still poorly ***,we described the development and validation of a 96-wells fluidic system,with interchangeable cell culture and fluidics,to perform high-throughput screenings under laminar high-flow *** demonstrated that endothelial cells in our newly developed 96-wells fluidic system respond to fluid flow-induced shear stress by aligning along the flow direction and increasing the levels of KLF2 and *** further demonstrate that our 96-wells fluidic system allows for efficient gene knock-down compatible with automated liquid handling for high-throughput screening ***,we propose that this modular 96-well fluidic system is an excellent platform to perform genome-wide and/or drug screenings to identify the molecular mechanisms involved in the responses of endothelial cells to high wall shear stress.

关键词： system laminar fluid

Effects of New and Aged Polyethylenterephthalat and Polylactic Acid on <i>Gammarus fossarum</i>(Crustacea: Amphipoda) during Long-Term Exposures

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j.urnal of Environmental Protection 2020年第5期11卷 359-376页

作者： Almut gerhardt LimCo International GmbH Konstanz Germany

The freshwater amphipod Gammarus fossarum (Koch, 1835) was exposed in four subsequent experiments of each 45 - 48 days to 1 cm2 plastic foil (Polyethylenterephthalat (PET), Polylactic acid (PLA)) with mortality, feeding, and behavior in the Multispecies Freshwater Biomonitor©(MFB) (spontaneous locomotion, gill ventilation) being studied once a week. Mortality was generally high and similar in all experiments, with a slight increase in PLA treatments. PLA exposed animals showed higher ventilation, but spontaneous locomotion was equally high in all treatments during all experiments, showing high reproducibility. Feeding rates reached up to 50% of the provided alder leaf in all treatments and in all experiments. PLA foils started to age by surface cracks, deformation at the edges and loss of transparency esp. in the 4th run with 135 d old foils. Both types of foils were equally colonized with biofilm during 45 days’ exposures, starting with ciliates and flagellates, followed by diatoms and green filamentous algae at last. Some taxa differed in absolute amounts in the 4 subsequent experiments due to seasonal succession. There was only marginal weight loss of the foils during 180 days of exposure. The biopolymer PLA started to degrade slowly after 180 d, whereas PET remained morphologically unchanged. The presence of the plastic foils in the beakers independent of their age had no significant effects on survival, behavior, and feeding of G. fossarum during 45 d of exposure.

关键词： Toxicity Plastic Biopolymer Biofilm Gammarus fossarum

Behavioural Responses of <i>Crangon crangon</i>(Crustacea, Decapoda) to Reduced Seawater pH Following Simulated Leakage from Sub-Sea Geological Storage

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j.urnal of Environmental Protection 2013年第7期4卷 61-67页

作者： gerhardt Almut Shaw Bamber 1International Research Institute of Stavanger (IRIS) Stavanger Norway 2LimCo International GmbH Konstanz Germany. International Research Institute of Stavanger (IRIS) Stavanger Norway

Carbon capture and storage (CCS) in sub-sea geological formations is being developed and promoted to mitigate CO2 discharges to the atmosphere from point sources such as power stations. There remain some questions on the risks associated with the possible loss of gas from storage and the environmental harm this could pose to marine organisms associated with the sea bed in these regions. This study investigated the effect of exposing the common shrimp (Crangon crangon) to reduced pH conditions and presents the results of stepwise pH-reductions (0.2 pH units from pH 7 down to pH 6). Behaviour was monitored continuously throughout 8 hours of exposure. In three subsequent experiments we could show a consistent and repeatable behavioural response pattern consisting of immediate avoidance reactions expressed as “shooting behaviour” following each pH-reduction every hour. The animals responded in a rapid manner to the shifts at all pH values, suggesting that these animals are sensitive to even relatively small changes. The results indicate that repeated acute pH-stress caused by CO2-leakage from carbon storage sites might affect the behaviour and subsequent fitness of natural populations of common shrimps. Changes in behaviour are likely to lead to increased predation on these animals and migration away from affected areas.

关键词： Behaviour Avoidance Shrimp pH CO2 Inj.ction CCS Acidification

Agrobacterium tumefaciens-mediated transformation of Eucalyptus urophylla clone BRS07-01

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j.urnal of Forestry Research 2020年第2期31卷 507-519页

作者： Gisela Manuela de Franca Bettencourt Carlos Ricardo Soccol Thais Salete Giovanella Luziane Franciscon Daiane Rigoni Kestring Isabel Rodrigues gerhardt j.liana Degenhardt-Goldbach Department of Bioprocess Engineering and Biotechnology Federal University of ParanaAvenida Cel.Francisco Heraclito dos Santos210CuritibaParanaBrazil Department of Agronomy Federal University of ParanaRua dos Funcionarios1540CuritibaParanaBrazil Embrapa Forestry Estrada da RibeiraKm 111ColomboParanaBrazil Embrapa Agricultural Informatics Av.Andre Tosello209CampinasSao PauloBrazil

Genetic transformation is becoming routine for engineering specific traits in important clones of recalcitrant species such as Eucalyptus;however,the efficiency is still low for most species,so many researchers still use seeds instead of clones as initial *** work aimed to develop a genetic transformation protocol,based on a highly efficient in vitro organogenesis protocol,for an Eucalyptus urophylla clone selected in our breeding *** growth regulators were evaluated for indirect organogenesis and *** a two-step protocol,the combination of callus induction media supplemented with 0.5 μM thidiazuron+0.5 μM naphthaleneacetic acid(NAA)and shoot induction media supplemented with 5.0 μM benzylaminopurine+1.0 lM NAA allowed up to 85.6%shoot formation with more shoots per explants when compared with other *** plants expressing the uidA gene were obtained using Agrobacterium tumefaciens and selected for kanamycin resistance.A RAPD analysis was used to check for somaclonal *** tests using 11 RAPD primers,we did not observe somaclonal variation in the in vitro stages evaluated.

关键词： Eucalyptus Genetic transformation In vitro regeneration Plant growth regulators Somaclonal variation

身体的“大理性”(上)--论查拉图斯特拉的第四篇演说

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现代哲学 2010年第3期 87-93页

作者： Volker gerhardt[德] 江晶静(译) 德国科学院联盟科研委员会北京大学北京100871

在查拉图斯特拉以“蔑视身体者”为题的第四篇演说中可以找到一个表达方式，它无比精辟地阐明了“存在”最为深邃庞大的谜题之一。它就是“身体的‘大理性＇”这一用语。

关键词：演说图斯身体理性 “存在” 深邃

身体的“大理性”(下)——论查拉图斯特拉的第四篇演说

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现代哲学 2010年第4期 72-79页

作者： Volker gerhardt[德] 江晶静（译）

查拉图斯特拉在给予蔑视身体的人以唯一忠告——这忠告完全可以通过上下文前后一致地推导出来，即让他们变成“哑巴”——之后，引用了一个孩子的言论，虽然他完全了解，这其实是大多数成年人会使用的说话方式：“‘我是身体和灵魂’—... 详细信息

查拉图斯特拉在给予蔑视身体的人以唯一忠告——这忠告完全可以通过上下文前后一致地推导出来，即让他们变成“哑巴”——之后，引用了一个孩子的言论，虽然他完全了解，这其实是大多数成年人会使用的说话方式：“‘我是身体和灵魂’——孩子如此说道。为什么人们不像孩子这样说呢？”（39）

关键词：身体图斯演说理性说话方式上下文孩子成年人

A Review of the DCP-DN Pavement Design Method for Low Volume Sealed Roads: Development and Applications

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j.urnal of Transportation Technologies 2019年第4期9卷 397-422页

作者： Philip Paige-Green gerhardt Daniel Van Zyl Department of Civil Engineering Tshwane University of Technology Pretoria South Africa Mycube Asset Management Systems (Pty) Ltd. Cape Town South Africa.

Widespread implementation of the DCP-DN design method for low volume roads has been promoted internationally over the past decade or so. The method has progressed from a simple determination of the in situ CBR investigation based on DCP-CBR correlations with respective cover requirements to a more sophisticated method using the DCP penetration data directly and omitting any need to use correlations with the CBR. This paper summarises the development of the method, and some of its advantages and compares the design structures with other recognised and widely implemented designs.

关键词： Low Volume Roads Design Dynamic Cone Penetrometer DCP CBR