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Straw return influences the structure and functioning of arbuscular mycorrhizal fungal community in a rice-wheat rotation system

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Pedosphere 2024年第2期34卷 339-350页

作者： Silong ZHAI Junjie XIE Zongyi TONG Bing YANG Weiping CHEN Roger Tkoide Yali MENG Xiaomin HUANG Atta Mohi Ud DIN Changqing CHEN Haishui YANG College of Agriculture Nanjing Agricultural UniversityNanjing 210095China Department of Biology Brigham Young UniversityProvo UT 84602USA Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology Agricultural College of Yangzhou UniversityYangzhou 225009China National Research Center of Intercropping The Islamia University of BahawalpurBahawalpur 63100Pakistan Jiangsu Collaborative Innovation Center for Modern Crop Production Nanjing Agricultural UniversityNanjing 210095China

Straw return is a sustainable soil fertility-building practice,which can affect soil microbial communities.However,how straw return affects arbuscular mycorrhizal fungi(AMF)is not well explored.Here,we studied the impacts of different straw management treatments over eight years on the structure and functioning of AMF communities in a rice-wheat rotation system.The straw management treatments included no tillage with no straw(NTNS),rotary tillage straw return(RTSR),and ditch-buried straw return(DBSR).The community structure of AMF was characterized using high-throughput sequencing,and the mycorrhizal functioning was quantified using an in situ mycorrhizal-suppression treatment.Different straw management treatments formed unique AMF community structure,which was closely related to changes in soil total organic carbon,available phosphorus,total nitrogen,ammonium,and nitrate.When compared with NTNS,RTSR significantly increased Shannon diversity in 0–10 cm soil layer,while DBSR increased it in 10–20 cm soil layer;DBSR significantly increased hyphal length density in the whole ploughing layer(0–20 cm),but RTSR only increased it in the subsurface soil layer(10–20 cm).The mycorrhizal responses of shoot biomass and nutrient(N and P)uptake were positive under both straw return treatments(RTSR and DBSR),but negative under NTNS.The community composition of AMF was significantly correlated to hyphal length density,and the latter was further a positive predictor for the mycorrhizal responses of plant growth and nutrient uptake.These findings suggest that straw return can affect AMF community structure and functioning,and farmers should manage mycorrhizas to strengthen their beneficial effects on crop production.

关键词： community structure hyphal length density shoot biomass shoot N uptake shoot P uptake soil physicochemical properties straw management tillage

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Identification of differentially expressed genes of primary spermatocyte against round spermatid isolated from human testis using the laser capture microdissection technique

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Cell Research 2004年第6期14卷 507-512页

作者： S.S.koide 1230 York Avenue Center for Biomedical Research Population Council NY 10021 New York USA

The method of laser capture microdissection (LCM) combined with suppressive subtractive hybridization (SSH) was developed to isolate specific germ cells from human testis sections and to identify the genes expressed during differentiation and development. In the present study, over 10,000 primary spermatocytes and round spermatid cells were successfully isolated by LCM. Using the cDNAs from primary spermatocytes and round spermatids, SSH cDNAs library of primary spermatocyte-specific was constructed. The average insert size of the cDNA isolated from 75 randomly picked white clones was 500 bp, ranging from 250 bp to 1.7 kb. Using the dot-blot method, a total of 421 clones were examined, resulting in the identification of 390 positive clones emitting strong signals. Partial sequence of cDNAs prepared from each clone was determined with an overall success rate of 84.4%. Genes encoding cytochrome c oxidase II and the rescue factor-humanin were most frequently expressed in primary spermatocytes, suggesting th

关键词： laser capture microdissection suppressive subtractive hybridization spermatogenesis cytochrome c oxidase Ⅱ humanin.

Evolution of the “Neutral” : Diverse Forms of Wide-Compatibility Haplotypes at the Locus for Reproductive Isolation

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Molecular Plant 2020年第4期13卷 539-540页

作者： Yohei koide Research Faculty of Agriculture Hokkaido UniversityKita 9 Nishi 9SapporoHokkaido 060-8589Japan

One of the biggest questions in evolutionary biology is how biological species arose.Since biological species are groups of interbreeding natural populations reproductively isolated from other such groups(Mayer,1942),reproductive isolation is the key process of the speciation.Among various isolating barriers,postzygotic barriers ranging from maladaptation to inviability or sterility in the offspring have attracted biologists’attention because of the problem of the evolution of the“unfit”offspring.A clear model to solve this problem is the Dobzhansky-Muller(DM)model(Figure 1;Coyne and Orr,2004),in which incompatibilities are generally caused by an interaction between the genes that have functionally diverged in each population without selection opposing any intermediate step(Coyne and Orr,2004).

关键词： Figure Forms breeding

Surface Activities of Calix [4]resorcinarenes Bearing Four Hydrophobic Chains and Its Solubilization for Organic Compounds

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Chemical Research in Chinese Universities 2002年第2期18卷 206-210页

作者： LI Bo Yoshifumi koide Kunio ESUMI Toyota Central R&D Labs. Inc.411YokomichiNagakuteAichi 4801192Japan Departemnt of Applied Chemistry and Institute of Colloid and Interface Science Science University of Tokyo

Calix resorcinarenes bearing four hydrophobic side chains ( Ar-Rn, Ar-Ph, and Ar-N) orient stably on water oil interface and show high solubilization capacities for organic compounds, such as long chain alcohols, benzene, toluene and dyes. The capacities are high even near the cmc of polyalkylated calix resorcinarenes, but the solubilities of organic compounds(solubilizates) decrease with the increase of the size of the solubilizates. Ar-R 6, with a chain length of six carbons, is the most effective among Ar-R n , Ar-Ph, and Ar-N; 11 fold mol of hexanol is dissolved in 2×10 -3 mol/L Ar-R 6. Moreover, Ar-R n bearing four alkyl side chains solubilizes the chain alcohols of the same chain length mostly. The solubilization capacities are presumably brought by inclusion in a large cavity of the polyalkylated calix resorcinarenes and by an efficient orientation of the solubilizates.

关键词： Calix resorcinarene Hydrophobic chain Polyalkylate Solubilizate

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Viabahn Stent Graft for Inadvertent Insertion of a Central Venous Catheter in the Subclavian Artery

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World Journal of Cardiovascular Diseases 2022年第7期12卷 397-402页

作者： Yuchen Cao Masaaki koide Masakazu Watanabe Division of Cardiovascular Surgery Seirei Hamamatsu General Hospital Hamamatsu Japan

Subclavian artery (SCA) injuries associated with central venous catheter (CVC) insertion are uncommon yet lethal complications that typically require surgical treatment. This case report presents the case of a 94-year-old man with an iatrogenic right SCA injury resulting from a misplaced CVC. Computed tomography revealed the catheter piercing the right internal jugular vein to enter the right SCA and then reaching the aortic arch. Emergent endovascular treatment was performed, and a 13-mm × 50-mm self-expanding Viabahn stent graft (W.L. Gore & Associates, Flagstaff, AZ, USA) was placed via the right brachial artery. The misplaced catheter was successfully removed under simultaneous postdeployment balloon dilatation. This case highlights the utility of the Viabahn stent graft for iatrogenic right SCA injury caused by a misplaced CVC and presents some insights and tips for a safer procedure.

关键词： Central Venous Catheter Insertion Iatrogenic Subclavian Artery Injury Viabahn Stent Graft Endovascular Treatment Surgical Techniques

Assessing the supercooling of fresh-cut onions at−5℃ using electrical impedance analysis

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Food Quality and Safety 2020年第2期4卷 55-58页

作者： Shoji koide Ami Yoneyama Takahiro Orikasa Matsuo Uemura Faculty of Agriculture Iwate UniversityMoriokaJapan Iwate Agri-Innovation Center Iwate UniversityMoriokaJapan

We supercooled fresh-cut onion at−5℃ for 12 h.After supercooling,the electric impedance properties of the samples were evaluated by electrical impedance spectroscopy over the frequency range of 42 Hz−5 MHz.The time-temperature profiles of samples indicated that the freezing point and supercooling point were−2.3℃±0.7℃ and−6.9℃±1.0℃,respectively.The results indicated that 34 of the 36 supercooled samples exhibited a definite circular arc in the Cole-Cole plot,which suggested that the cell membrane remained intact during supercooling.In the other two samples which did not exhibit a definite circular arc,the cell membrane had sustained serious damage during supercooling.Furthermore,there was large difference in drip loss percentage between supercooled samples exhibited a definite circular arc in the Cole-Cole plot and samples not exhibiting a definite circular arc.Our results suggest that fresh-cut onions can be supercooled at−5℃.

关键词： supercooling fresh-cut onion electrical impedance Cole-Cole plot supercooling point

Impact of blanching pretreatment on the drying rate and energy consumption during far-infrared drying of Paprika(Capsicum annuum L.)

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Food Quality and Safety 2018年第2期2卷 97-103页

作者： Takahiro Orikasa Naoki Ono Takashi Watanabe Yasumasa Ando Takeo Shiina Shoji koide Faculty of Agriculture Iwate UniversityMorioka IARC Kenpoku Agricultural Research Institute KunoheIwate Food Research Institute NAROTsukubaIbaraki Graduate School of Horticulture Chiba UniversityMatsudoJapan

We incorporated a superheated steam blanching pretreatment step into a paprika drying process and compared the far-infrared(FIR)drying rates,hardness of the sample surfaces,cell membrane stabilities,and energy consumption of blanched and non-blanched paprika.The average drying rate of blanched paprika samples during FIR drying was higher than that of non-blanched samples.The hardness and cell membrane stability of dried blanched samples were lower than those of non-blanched samples.We estimated that the softening of the sample surfaces and injury to the cell membranes caused the drying rate to increase.The total energy consumption of the FIR drying of paprika was reduced by approximately 30%by introducing the blanching pretreatment.These findings contribute to the development of environmentally friendly FIR drying techniques for paprika.

关键词： blanching cell membrane stability,energy consumption far-infrared drying hardness.

Cloning of rat sp56, the homologue of mouse sperm ZP3 receptor-sp56

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Cell Research 2003年第2期13卷 121-129页

作者： SS koide The Population Council Center for Biomedical Research 1230 York Avenue New York New York 100021 USA

Mouse sp56 is considered as one of the candidates for mouse zona pellucida 3 (mZP3) receptor. Up to date, its homologue has only been cloned from guinea pig. namely AM67. Based on the cDNA sequence of mouse sp56, we designed a pair of primer to amplify its homologue from rat testis cDNA. Using RT-PCR, two fragments of 743 bp and 938 bp were amplified. The PCR products show very high homology to mouse sp56. However, the 743 bp product completely lacks one of the seven Sushi domains of mouse sp56. Using the 743 bp product as the probe to detect the expression profile of sp56 in rat tissues, Northern blot shows that a -2.0 kb mRNA expresses specifically in testis. Employed the RACE method, two full cDNA sequences of rat sp56 were obtained. A Mr -42 KD band was detected in denatured and non-reducing protein sample of rat testis and sperm with anti-mouse sp56 monoclonal antibody by Western blot method. Rat sp56 was localized on rat sperm head by the indirect immunofluorescence method. Rat sp56 immunoreactivi

关键词： ZP3 receptor sp56 rat testis sperm cloning.

SEISMIC WAVE VELOCITY MONITORING OF CO_2 MIGRATION IN POROUS SANDSTONES SATURATED WITH WATER

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岩石力学与工程学报 2003年第6期22卷 1002-1007页

作者： OhsumiTakashi koideHitoshi Research Institute of Innovative Technology for the Earth Kyoto 6109-0292 JapanResearch Institute of Innovative Technology for the EarthKyoto 6109-0292 Japan

Laboratory experiments were conducted to measure the compressional wave velocities under hydrostatic pressure in Shirahama Tako sandstones with a porosity of 12% and 24%,respectively.In dry samples,the pressure dependence of velocity is ascribed to the difference in pore structures.Velocities were also measured to map the movement of the injected CO2 within water-saturated samples during CO2 injection.In the water-saturated samples,velocity changes caused by the CO2 injection are typically on the order of 10%.A series of seismic tomography experiments are conducted on porous sandstone samples to demonstrate the use of cross-well seismic profiling for monitoring the migration of CO2 in geological sequestration projects.

关键词： CO2 弹性波检测饱和砂岩流动性

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