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Morphology and gene expression of sex‐determining region Y‐box 9 and runt‐related transcription factor 2 in centrifugally compressed cell collagen‐combined constructs (C^(6))

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Biosurface and Biotribology 2023年第3期9卷 78-83页

作者： Xu Ye masashi Yamazaki Wataru Ando Norimasa Nakamura Hiromichi Fujie Faculty of System Design Tokyo Metropolitan UniversityTokyoJapan Research Center for Medicine‐Engineering Collaboration TokyoJapan Department of Orthopaedic Surgery Kansai Rosai HospitalAmagasakiJapan Department of Orthopaedic Surgery Osaka University Graduate School of MedicineSuitaJapan Institute for Medical Science in Sports Osaka Health Science UniversityOsakaJapan Center for Advanced Medical Engineering and Informatics Osaka UniversitySuitaJapan

The authors previously developed a scaffold‐free tissue‐engineered construct(TEC)from mesenchymal stem cells(MSCs).Although the TEC exhibited even cell distribution and was successfully applied for cartilage repair in animal models,it is unsuitable for relatively large‐scale cartilage defects due to its small *** solve the problem,the authors recently developed a novel biomaterial,a centrifugally compressed cell‐collagen combined construct(C^(6))from a mixture of MSCs and atelocollagen,both of which are subjected to *** results of the previous study indicated that C^(6) exhibited high cell viability(70%)and sufficient cell distribution similar to that of the *** the present study,the morphology and gene expression of C^(6) were *** ex-amination indicated that C6 is six times thicker(approximately 1 mm)than the TEC after a 7‐day *** C^(6) remained unchanged in scale with increased cell density after a 21‐day *** electron microscopic observation indicated that C^(6) exhibited interconnected and porous microstructures,while the TEC had close‐knit *** transcriptase‐polymerase chain reaction analysis indicated that the expression of sex‐determining region Y‐box 9 and runt‐related transcription factor 2 was significantly higher in C^(6) than that in TEC.

关键词： biomaterials cartilage microstructures

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Investigation and prediction of enteral nutrition problems after percutaneous endoscopic gastrostomy

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World Journal of Gastroenterology 2009年第11期15卷 1367-1372页

作者： Shiro Yokohama Masaru Aoshima Yukiomi Nakade Junya Shindo Junichi Maruyama masashi Yoneda Department of Gastroenterology Asahikawa Rehabilitation HospitalAsahikawa078-8801Japan Division of Gastro-enterology Department of Internal MedicineAichi Medical University School of MedicineAichi 480-1195Japan Department of Rehabilitation Asahikawa Rehabilitation HospitalAsahikawa 078-8801Japan Department of Internal Medicine Asahikawa Rehabilitation HospitalAsahikawa 078-8801Japan

AIM： To investigate and predict enteral nutrition problems after percutaneous endoscopic gastrostomy （PEG）. METHODS： We retrospectively analyzed data for 252 out of 285 patients who underwent PEG at our hospital from 1999 to 2008 after PEG were defined as： Enteral nutrition problems （1） patients who required ≥ 1 mo after surgery to switch to complete enteral nutrition, or who required additional parenteral alimentation continuously; or （2） patients who abandoned switching to enteral nutrition using the gastrostoma and employed other nutritional methods. We attempted to identify the predictors of problem cases by using a logistic regression analysis that examined the patients＇ backgrounds and the specific causes that led to their problems. RESULTS： Mean age of the patients was 75 years, and in general, their body weight was low and their overall condition was markedly poor. Blood testing revealed that patients tended to be anemic and malnourished. A total of 44 patients （17.5%） were diagnosed as having enteral nutrition problems after PEG. Major causes of the problems included pneumonia, acute enterocolitis （often Clostridium difficile-related）, paralytic ileus and biliary tract infection. A multivariate analysis identified the following independent predictors for problem cases：（1） enteral nutrition before gastrectomy （a risk reduction factor）; （2） presence of esophageal hiatal hernia; （3） past history of paralytic ileus; and （4） presence of chronic renal dysfunction. CONCLUSION： Enteral nutrition problems after PEG occurred at a comparatively high rate. Patient background analysis elucidated four predictive factors for the problem cases.

关键词： Percutaneous endoscopic gastrostomy Enteral nutrition Complication, Risk factor Predictor

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Determination of the Dzyaloshinskii-Moriya interaction using pattern recognition and machine learning

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npj Computational Materials 2021年第1期7卷 198-204页

作者： masashi Kawaguchi Kenji Tanabe Keisuke Yamada Takuya Sawa Shun Hasegawa Masamitsu Hayashi Yoshinobu Nakatani Department of Physics The University of TokyoTokyo 113-0033Japan Toyota Technological Institute Nagoya 468-8511Japan Department of Chemistry and Biomolecular Science Faculty of EngineeringGifu UniversityGifu 501-1193Japan University of Electro-Communications Tokyo 182-8585Japan

Machine learning is applied to a large number of modern devices that are essential in building an energy-efficient smart *** and face recognition are among the most well-known technologies that make use of such artificial *** materials research,machine learning is adapted to predict materials with certain functionalities,an approach often referred to as materials ***,we show that machine learning can be used to extract material parameters from a single image obtained in *** Dzyaloshinskii–Moriya(DM)interaction and the magnetic anisotropy distribution of thin-film heterostructures,parameters that are critical in developing next-generation storage class magnetic memory technologies,are estimated from a magnetic domain *** simulation is used to generate thousands of random images for training and model validation.A convolutional neural network system is employed as the learning *** DM exchange constant of typical Co-based thin-film heterostructures is studied using the trained system:the estimated values are in good agreement with ***,we show that the system can independently determine the magnetic anisotropy distribution,demonstrating the potential of pattern *** approach can considerably simplify experimental processes and broaden the scope of materials research.

关键词： pattern interaction system

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Operational efficiencies and costs of an arm roll forwarder: A case study at Nasu in Tochigi Prefecture, Japan

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Journal of Forestry Research 2013年第3期24卷 577-583页

作者： Kazuhiro Aruga Yuto Otsuka Chikara Nakahata Reiko Yamaguchi masashi Saito Mayu Nagasaki Department of Forest Science Faculty of Agriculture Utsunomiya University350 Mine Utsunomiya 321-8505 Japan Frontier Agriscience and Technology Center Faculty of Agriculture Shinshu UniversityNagano 399-4598 Japan Japan Wood Energy Co. Ltd.Tokyo 198-0036

We investigated a developed arm roll forwarder at Nasu in Tochigi Prefecture, Japan. An arm roll forwarder can only load a steel container that has been fully loaded with logs beforehand, and can later unload such a container. Such a forwarder can shorten the loading and untoading times and improve operational efficiency. We examined two operation systems with an arm roll forwarder and a forwarder. In the first system the loading was done with a grapple-loader. In the second system the loading was done with a processor. The loading times of an arm roll forwarder were significantly tess than those of a forwarder. Because the optimal cycle times （excluding the waiting times for an arm roll for- warder） were significantly reduced, the costs of using an arm roll for- warder are lower, although the loading capacity was small and the hourly operation cost was high. The maximum operational efficiencies varied depending on forwarding distances. The second operation system with an arm roll forwarder exhibited the best operational efficiency within a 1,580-m forwarding distance, and beyond that distance it exhibited the highest operational efficiency when a forwarder was used. Similarly, the cost of operation of the system with an arm roll forwarder was the lowest within a 1,130-m forwarding distance, and beyond that distance the cost was the lowest when using the forwarder. Therefore, the arm roll for- warder is effective within a certain forwarding distance.

关键词： arm roll forwarder steel container operational efficiency cost forwarding distance

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Characterization of the genes involved in nitrogen cycling in wastewater treatment plants using DNA microarray and most probable number-PCR

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Frontiers of Environmental Science & Engineering 2016年第4期10卷 61-70页

作者： Junqin PANG Masami MATSUDA masashi KURODA Daisuke INOUE Kazunari SEI Kei NISHIDA Michihiko IKE Division of Sustainable Energy and Environmental Engineering Graduate School of Engineering Osaka University 2-1 Yamadaoka Suita Osaka 565-0871 Japan International Research Centre for River Basin Environment University of Yamanashi 4-3-11 Takeda Kofu Yamanashi 400-8511 Japan

To improve nitrogen removal performance of wastewater treatment plants （WWTPs）, it is essential to understand the behavior of nitrogen cycling communities, which comprise various microorganisms. This study characterized the quantity and diversity of nitrogen cycling genes in various processes of municipal WWTPs by employing two molecular-based methods：most probable number-polymerase chain reaction （MPN-PCR） and DNA microarray. MPN-PCR analysis revealed that gene quantities were not statistically different among processes, suggesting that conventional actwated sludge processes （CAS） are similar to nitrogen removal processes in their ability to retain an adequate population of nitrogen cycling microorganisms. Furthermore, most processes in the WWTPs that were researched shared a pattern：the nitS and the bacterial amoA genes were more abundant than the nirK and archaeal amoA genes, respectivelv. DNA microarray analysis revealed that several kinds of nitrification and denitrification genes were detected in both CAS and anaerobic-oxic processes （AO）, whereas limited genes were detected in nitrogen removal processes. Results of this study suggest that CAS maintains a diverse community of nitrogen cycling microorganisms; moreover, the microbial communities in nitrogen removal processes may be specific.

关键词： DNA microarray analysis Nitrogen cycling functional genes Most probable number-polymerase chainreaction (MPN-PCR)Wastewater treatment plants (WWTPs)

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Osmotic pressure of serum and cerebrospinal fluid in patients with suspected neurological conditions

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Neural Regeneration Research 2020年第5期15卷 944-947页

作者： Tetsuya Akaishi Toshiyuki Takahashi Ichiro Nakashima Michiaki Abe masashi Aoki Tadashi Ishii Department of Education and Support for Regional Medicine Tohoku University HospitalSendaiJapan Department of Neurology Tohoku University Graduate School of MedicineSendaiJapan Department of Neurology National Hospital Organization Yonezawa National HospitalYonezawaJapan Department of Neurology Tohoku Medical and Pharmaceutical UniversitySendaiJapan

Interstitial fluid movement in the brain parenchyma has been suggested to contribute to sustaining the metabolism in brain parenchyma and maintaining the function of neurons and glial *** pulsatile hydrostatic pressure gradient may be one of the driving forces of this bulk ***,osmotic pressure- related factors have not been studied until *** this prospective observational study,to elucidate the relationship between osmolality (mOsm/kg) in the serum and that in the cerebrospinal fluid (CSF),we simultaneously measured the serum and CSF osmolality of 179 subjects with suspected neurological *** osmolality was 283.6 ± 6.5 mOsm/kg and CSF osmolality was 289.5 ± 6.6 mOsm/*** the specific gravity of serum and CSF is known to be 1.024–1.028 and 1.004–1.007,respectively,the estimated average of osmolarity (mOsm/L) in the serum and CSF covered exactly the same range (i.e.,290.5–291.5 mOsm/L).There was strong correlation between CSF osmolality and serum osmolality,but the difference in osmolality between serum and CSF was not correlated with serum osmolality,serum electrolyte levels,protein levels,or quotient of *** conclusion,CSF osmolarity was suggested to be equal to serum *** is not one of the driving forces of this bulk *** factors such as hydrostatic pressure gradient should be used to explain the mechanism of bulk flow in the brain *** study was approved by the Institutional Review Board of the Tohoku University Hospital (approval *** No.2015-1-257) on July 29,2015.

关键词： brain parenchyma bulk flow cerebrospinal fluid hydrostatic pressure interstitial fluid osmolarity osmotic pressure

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Analysis of lichen substances including triterpenoids by high performance liquid chromatography with a differential refractive index detector and a photodiode array detector

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菌物学报 2011年第6期30卷 944-949页

作者： Hikari SATO Kojiro HARA masashi KOMINE Yoshikazu YAMAMOTO Graduate School of Bioresource Sciences Akita Prefectural University:Shimoshinjo-nakanoAkita 010-0195Japan

A new method for analysis of lichen triterpenoids was established using high performance liquid chromatography with the combination of a differential refractive index detector(RID) and a photodiode array detector(PDA).It is proved that this method was convenient to detect and identify aromatic and aliphatic lichen substances;it enabled quantitative analysis of substances having no or less absorption of ultraviolet rays such as *** addition,they can be measured in high accuracy compared with the TLC method.

关键词：三萜系化合物青苔 RID 菌物学

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Moving computational multi-domain method for modelling the flow interaction of multiple moving objects

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Advances in Aerodynamics 2022年第1期4卷 78-95页

作者： Momoha Nishimura masashi Yamakawa Shinichi Asao Seiichi Takeuchi Mehdi Badri Ghomizad Department of Mechanical and System Engineering Kyoto Institute of TechnologyKyotoJapan Department of Mechanical Engineering College of Industrial TechnologyHyogoJapan

This study proposes a method for modelling the flow interaction of multiple moving objects where the flow field variables are communicated between multiple separate moving computational *** of using the conventional approach with a single fixed computational domain covering the whole flow field,this method advances the moving computational domain(MCD)method in which the computational domain itself moves in line with the motions of an object *** computational domains created around each object move independently,and the flow fields of each domain interact where the flows *** eliminates the spatial restriction for simulating multiple moving ***,a shock tube test verifies that the overset implementation and grid movement do not adversely affect the results and that there is communication between the grids.A second test case is conducted in which two spheres are crossed,and the forces exerted on one object due to the other’s crossing at a short distance are *** results verify the reliability of this method and show that it is applicable to the flow interaction of multiple moving objects.

关键词： Crossing Moving boundary problems Compressible flow Moving mesh method

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Safety and effectiveness of propofol sedation during and after outpatient colonoscopy

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World Journal of Gastroenterology 2012年第26期18卷 3420-3425页

作者： Akira Horiuchi Yoshiko Nakayama masashi Kajiyama Naoyuki Kato Tetsuya Kamijima Yasuyuki Ichise Naoki Tanaka Digestive Disease Center Showa Inan General HospitalKomagane 399-4117Japan Department of Pediatrics Shinshu University School of MedicineMatsumoto 390-8621Japan Department of Metabolic Regulation Shinshu University Graduate School of MedicineMatsumoto 390-8621Japan

AIM:To study the safety and effectiveness of propofol sedation for outpatient ***:Propofol was given by bolus injection with an age-adjusted standard protocol consisting of 60 mg for patients < 70 years old,40 mg for patients age 70-89 years,and 20 mg for those ≥ 90 years,and additional injections of 20 mg propofol were given up to a maximum of 200 *** principal parameters were the occurrence of adverse events within 24 h after colonoscopy and overall satisfaction for this *** parameters included successful procedure,respiratory depression,and other ***:Consecutive patients were entered prospectively and all 2101 entered successfully completed outpatient *** mean dose of propofol used was 96.4 mg(range 40-200 mg).Younger patients required higher doses of propofol than older patients(20-40 years vs ≥ 61 years:115.3 ± 32 mg vs 89.7 ± 21 mg,P < 0.001).Transient supplemental oxygen supply was needed by five patients(0.2%);no other complications *** questionnaires were completed by 1820(87%) of 2101 patients and most rated their overall satisfaction as excellent(80%) or good(17%).The majority(65%) of patients drove home or to their office after their ***(99%) were willing to repeat the same *** incidents occurred within 24 h after ***:Propofol sedation using a dose < 200 mg proved both safe and practical for outpatient colonoscopy.

关键词： Colonoscopy Propofol Colorectal cancer

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Karyotypes of field mice of the genus Apodemus (Mammalia： Rodentia) from China

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Zoological Research 2018年第5期39卷 348-355页

作者： Masaharu Motokawa Yi Wu masashi Harada Yuta Shintaku Xue-Long Jiang Yu-Chun Li Kyoto University Museum Kyoto University Kyoto 606-8501 Japan Key Laboratory of Conservation and Application in Biodiversity of South China School of Life Sciences Guangzhou University Guangzhou Guangdong 510006 China Laboratory Animal Center Graduate School of Medicine Osaka City University Osaka 545-8585 Japan Wildlife Research Center Kyoto University Sakyo Kyoto 606-8203 Japan Japan Monkey Centre Inuyama Aichi 484-0081 Japan Kunming Institute of Zoology Chinese Academy of Sciences Kunming Yunnan 650223 China Marine College Shandong University(Weihai) Weihai Shandong 264209 China

Karyotypes of four Chinese species of field mice of the genus Apodemus were examined,including Apodemus chevrieri （diploid chromosome number,2n=48,fundamental number of autosomal arms,FNa=56）,*** （2n=48,FNa=48）,*** （2n=48,FNa=48）,and *** （2n=48,FNa=48）.Karyotypes of ***,***,and *** are reported here for the first time,providing useful information for their species *** the karyotypes of all species of Apodemus in Asia,both in this and previous studies,provides a solid overview of the chromosome evolution and species differentiation of the genus in East *** addition to allopatric speciation,chromosome rearrangements likely played an important role in the formation of the four Apodemus species groups as well as speciation within each group in East *** example,increased centromeric heterochromatin in *** may have contributed to the post-mating reproductive isolation from the *** clade.

关键词： Karyotype Chromosome evolution Speciation Taxonomy Field mice

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