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Proteomic profile of human monocytic cells infected with dengue virus

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Asian Pacific Journal of Tropical Biomedicine 2016年第11期6卷 914-923页

作者： Viviana mart.nez-Betancur Marleín mart.nez-Gutierrez Programa de Estudio y Control de Enfermedades Tropicales-PECET Universidad de Antioquia Grupo de Investigación en Ciencias Animales-GRICA Facultad de Medicina Veterinaria y Zootecnia Universidad Cooperativa de Colombia

Objective: To identify the changes in the proteome of U937 cells infected with dengue virus(DEnV).Methods: In this study, differentiated U937 cultures were infected with two DEnV-2strains, one of which was associated with dengue(DEnV-2/nG) and the other one with severe dengue(DEnV-2/16681), with the aim of determining the cellular proteomic profiles under different infection conditions. Cellular proteins were extracted and separated by two-dimensional electrophoresis, and those proteins with differential expression profiles were identified by mass spectrometry. The obtained results were correlated with cellular viability, the number of infectious viral particles, and the viral DnA/protein ***: In comparison with non-infected cultures, in the cells infected with the DEnV-2/nG strain, nine proteins were expressed differentially(five were upregulated and four were downregulated); in those cultures infected with the DEnV-2/16681 strain, six proteins were differentially expressed(two were downregulated and four were upregulated). The downregulated proteins included fatty acid-binding protein, heterogeneous nuclear ribonucleoprotein 1, protein disulfide isomerase, enolase 1, heat shock 70 k Da protein 9, phosphotyrosyl phosphatase, and annexin IV. The upregulated proteins included heat shock 90 k Da protein AA1, tubulin beta, enolase 1, pyruvate kinase,transaldolase and phospholipase ***: Because the monocyte/macrophage lineage is critical for disease pathogenicity, additional studies on these proteins could provide a better understanding of the cellular response to DEnV infection and could help identify new therapeutic targets against infection.

关键词： Dengue virus Proteomic Macrophages Monocytes U937 cells

A new player in the beneficial effects of exercise on the aged brain

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Signal Transduction and Targeted Therapy 2020年第1期5卷 901-902页

作者： M.Llorens-mart.n Department of Molecular neuropathology Centro de Biología Molecular“Severo Ochoa”CBMSOCSIC-UAMMadridSpain Center for networked Biomedical Research on neurodegenerative Diseases(CIBERnED) MadridSpain Department of Molecular Biology Faculty of SciencesUniversidad Autónoma de MadridMadridSpain

In a recent article in Science,Horowitz et *** glycosylphosphatidylinositol(GPI)-degrading enzyme Gpld1 as the most critical player in the blood that can transfer the effects of exercise on adult neurogenesis and cognition to sedentary aged *** also demonstrate the importance of this liver-to-brain axis in ameliorating age-related regenerative and cognitive impairments.

关键词： tar player critical

Main factors affecting lacertid lizard thermal ecology

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Integrative Zoology 2019年第3期14卷 293-305页

作者： Zaida ORTEGA Francisco Javier mart.n-VALLEJO Postgraduate Program in Ecology and Conservation Federal University of Mato Grosso do SulCampo GrandeMato Grosso do SulBrazil Department of Statistics University of SalamancaSpain

The thermal ecology of ectotherms has been studied for almost 2 centuries,but additional attention is currently being paid to it,to understand how organisms deal with the environment in a climate change context.A consensus is still far away due to the large number of factors involved and their complex *** this context,3 analyses in lacertid lizards were carried out:(i)a meta-analysis,to test for differences between body and air temperatures from 71 populations;(ii)a meta-analysis concerning correlations between body and air temperatures from 60 populations;and(iii)a multimodel inference of thermoregulation effectiveness indices from 45 *** importance of different factors,including body size,habitat,insularity,altitude,climate and season,was evaluated in all analyses to model the response variables.A strong seasonality effect was observed,with a consistent pattern of less effective thermoregulation in summer compared to other *** was the second most important factor,with a consistent higher thermoregulation effort in populations occurring at high elevations(>1000 m above sea level).Other factors,such as insularity or body size,can also be important,but did not exhibit a clear ***,thermoregulation was less affected by climate and habitat type.

关键词： lizards meta-analysis seasonality temperature thermoregulation

neuroprotective therapy for retinal neurodegenerative diseases by stem cell secretome

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neural Regeneration Research 2021年第1期16卷 117-118页

作者： Ricardo Usategui-mart.n Ivan Fernandez-Bueno Instituto Universitario de Oftalmobiología Aplicada(IOBA) Retina GroupUniversidad de ValladolidValladolidSpain Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León Red Temática de Investigación Cooperativa en SaludOftaredInstituto de Salud Carlos IIIValladolidSpain

Retinal neurodegenerative diseases like agerelated macular degeneration,glaucoma,diabetic retinopathy or retinitis pigmentosa are the most frequent causes of incurable low vision and blindness *** had been estimated that the prevalence of these diseases varies between 1/750 and 1/5000 depending on the region,the level of consanguinity or ethnicity(na et al.,2017).The functional and structural complexity of the retina makes it susceptible to multiple types of pathogenic damage.

关键词： diseases protective degenerative

Effect of insoluble materials on the volumetric behavior of rock salt

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Journal of Rock Mechanics and Geotechnical Engineering 2021年第1期13卷 84-97页

作者： Mejda Azabou Ahmed Rouabhi Laura Blanco-mart.n MInES ParisTech PSL Research UniversityCentre de Géosciences35 rue St HonoréFontainebleau77300France

This paper focuses on the presence of nodules of insoluble materials within salt specimens,and their effect on the volumetric strain measurements and the dilatancy *** analyzed experimental results of over 120 conventional triaxial compression tests,and found that in 20%of the cases,the volumetric strain measurements were *** also noted that the natural variability of the specimens can lead to a non-negligible data scattering in the volumetric strain measurements when different specimens are subjected to the same *** is expected given the small magnitude of those strains,but it occasionally implies that the corresponding specimens are not representative of the volumetric behavior of the studied *** order to understand these results,we numerically investigated salt specimens modeled as halite matrices with inclusions of *** of triaxial compression tests on these structures proved that such heterogeneities can induce dilatancy,and their presence can lead to the appearance of tensile zones which is physically translated into a micro-cracking *** modeling approach is validated as the patterns displayed in the numerical results are identical to that in the *** was then employed to explain the observed irregularities in experimental *** studied the natural variability effect as well and proposed a methodology to overcome the issue of specimen representativity from both deviatoric and volumetric perspectives.

关键词： Rock salt Dilatancy Material heterogeneity natural variability Triaxial tests Virtual laboratory

Does forest restoration influence soil infiltrability? A case study in the restored woodland of Sierra de ávila(Central Spain)

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Journal of Mountain Science 2021年第7期18卷 1778-1793页

作者： MOnGIL-MAnSO Jorge nAVARRO-HEVIA Joaquín SAn mart.n Roberto Hydrology and Conservation Research Group Catholic University“Santa Teresa de Jesús”ofÁvilaÁvila 05005Spain Forest Water and Soil Research GroupPalencia 34004Spain Department of Agricultural and Forestry Engineering University of ValladolidPalencia 34004Spain Department of Statistics and Operations Research University of ValladolidPalencia 34004Spain

Infiltration,as a major component of the hydrological cycle,plays an important role in ecosystems,river flooding,and soil ***,this process has been studied on different soils,with different vegetation cover,and under different climate ***,it is still necessary to know how infiltration rates depend on land degradation,vegetation cover,forest management,and forest restoration,since soil infiltration is related to soil hydrological function and hydrological ecosystem *** aim of our study is to analyze the way reforestation and check dam construction have helped to improve soil infiltration rates in comparison with old,degraded land,different soils and vegetation covers in Central ***,three infiltration tests were carried out by means of a simple methacrylate infiltrometer ring,in four sampling plots,for five types of land use:(i) native holm oak forest,(ii) 60-year-old reforested pine wood,(iii) shrubs,(iv) sediment wedges of check dams,and(v) gullies and degraded *** results show much higher infiltration rates in the soil of 60-yearold pine reforestation sites(1198.00 mm·h^(-1)),and in the sediment wedges of check dams(1088.00 mm·h^(-1)),than in those of degraded hillslopes(365.00 mm·h^(-1)) and shrubland(420.80 mm·h^(-1)).The rates were also shown to be close to those from the remaining patches of native holm oak woodland(770.40 mm·h^(-1)).We also found that organic matter,humus and litter depth,and height of vegetation and cover,all improve soil infiltration rates,while slope degree,presence of coarse elements,stoniness,clay content,bulk density,and electric conductivity inhibit the *** was additionally seen that pine reforestation and check dam construction caused degraded land to recover its hydrological conditions to a level that is quite close to that of the ancient oak holm native forest,alongside ameliorating the hydrological cycle in the *** information will be very useful for decisi

关键词： Soil hydrology Permeability Runoff Reforestation Land restoration

Habitat and food preferences of European rabbits in core and edge populations along the invasion front PatagoniaMonte,Argentina

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Current Zoology 2024年第3期70卷 310-319页

作者： Sabrina Yasmin Bobadilla María A.Dacar Fabián M.Jaksic Ricardo A.Ojeda María Fernanda Cuevas Ecología de Mamíferos de Tierras Secas IADIZACCT-Mendoza CONICETAv.Ruiz Leal s/nParque General San MartínMendoza 5500MendozaArgentina Laboratorio de Filogeografía Taxonomía Integrativa y Ecología(LFTIE)IADIZACCT-Mendoza CONICETAv.Ruiz Leal s/nParque General San MartínMendoza 5500MendozaArgentina Center of Applied Ecology and Sustainability(CAPES) Pontifcia Universidad Católica de ChileAv.Libertador Bernardo O'Higgins 340Santiago 8320000Región MetropolitanaChile

The European rabbit Oryctolagus cuniculus is an exotic herbivorous mammal undergoing an active phase of geographical expansion in the arid ecosystems of *** Adaptive Flexibility Hypothesis states that populations at the range edge(new populations)will exhibit greater fexibility in the use of resources compared with populations located in the range core(older populations).The objective of this work was to compare the rabbit’s use of spatial and trophic resources in relation to the establishment time of their *** sampling was carried out for 2 years(2017 and 2018)in sites with different establishment times for rabbit *** sampling stratifed by type of habitat was applied using 115 fxed strip transects of 1,000 m^(2) laid out across the study *** rabbit signs were recorded in each transect,and environmental and anthropic variables were *** results show that the individuals from the range edge are more selective in the use of habitat than those from the range *** the microhabitat level,we observed a pattern in the particular components of habitat use by rabbits mainly linked to food availability and proximity to *** a trophic perspective,rabbits could show fexible adjustment to novel conditions and environments in the range *** variability in resource use by the European rabbit confrms its ecological fexibility,pivotal for their advance toward new environments in Argentina.

关键词： Arid ecosystems invasive species novel environments range invasion spatial use trophic use

Mind the gap among patches in arid plant communities:rapid root proliferation in response to n addition

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Journal of Plant Ecology 2019年第1期12卷 89-95页

作者： Maria Fernanda Reyes mart.n RAguiar Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura(IFEVA)and Consejo nacional de Investigaciones Científicas y Técnicas(COnICET) Universidad de Buenos AiresAv.San Martín 4453Buenos Aires C1417DSEArgentina

Aims In arid communities,it has been proposed that individual plants can extend their roots beyond their canopy exploring neighbour-ing bare ground *** becomes relevant in systems where the vegetation is distributed in patches surrounded by bare ***,whether roots of different species may be overlapping under bare ground areas is still *** factors control-ling root responses when no plants appear to be directly influ-encing the gap among patches are still *** aim of our study was to detect perennial grasses responses to an n enrich-ment *** In a semi-arid steppe(Patagonia,Argentina),we buried root traps filled with sieved soil with and without n addition,under bare soil *** were harvested after 4 and 6 *** neighbour-hoods(30 cm in diameter)included at least three of the dominant tussock *** harvests,we identified species in the traps by root traits and quantified diversity,biomass and specific relative growth *** Findings Bare ground areas show simultaneous root growth of different *** of perennial grass roots was higher with n addition than without it in the first harvest(4 months),but this difference disappeared in the second harvest(6 months).Root biomass was maximal after 6 months in n addition *** preferred by herbivores(Bromus pictus and Poa ligularis)showed rapid growth and responses to n *** between harvests may be an indicative that n pulses interact with rising temperatures and soil water content as growing season progress.

关键词： belowground community ecology grass species roots Patagonian steppe patchiness root growth rates zone of influence

The role of Islet-1 in cell specification,differentiation,and maintenance of phenotypes in the vertebrate neural retina

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neural Regeneration Research 2015年第12期10卷 1951-1952页

作者： Gervasio mart.n-Partido Javier Francisco-Morcillo Departamento de Biología Celular Facultad de CienciasUniversidad de Extremadura

Many blinding diseases,such as retinitis pigmentosa,age-related macular degeneration,and glaucoma involve the permanent loss of retinal neurons,especially photoreceptors or the centrally projecting retinal ganglion **... 详细信息

关键词： cell RGCs The role of Islet-1 in cell specification,differentiation,and maintenance of phenotypes in the vertebrate neural retina