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Journal of Forestry Research 2020年第6期31卷 2287-2293页

作者： Silvio J.Ramos Markus Gastauer Simone K.Mitre cecı´lio F.caldeira Joyce R.Silva Antonio E.Furtini Neto Guilherme Oliveira Pedro W.M.souza filho Jose´O.Siqueira Instituto Tecnolo´gico Vale Rua Boaventura da Silva955Belem 66055-090Brazil departamento de ciencia do Solo Universidade Federal de LavrasLavras 37200-000Brazil

The primary challenge of mineland revegetation is the establishment of species able to cope with low availability of nutrients,especially in steep slopes such as of mine *** evaluated plant growth response and nutrient use efficiency(NUE)of two promising native Fabaceae species(Dioclea apurensis—liana from metalliferous savannas;Bauhinia longipedicellata—tree from Amazon rainforest)from the caraja´s Mineral Province,eastern *** were grown separately in 2-kg pots filled with mining *** were fertilized with nitrogen,phosphorus,potassium(NPK),lime,and *** results showed increments on growth of both species when nutrients were applied to the mining *** showed increases in leaf area,plant height,stem diameter,and shoot dry mass production when NPK or NPK?micronutrients were applied,while *** was responsive to application of NPK?lime or NPK?lime?***,*** showed higher NUE than ***,especially at the lowest doses of N,P and *** findings may indicate a substantial advantage of *** for mineland revegetation,as this species may require lower nutrient inputs,being,therefore,a more sustainable way to revegetate degraded areas.

关键词： Fertilizer application Mining soil Tropical forest Nutrient use efficiency

Where Are the Bromeliads? A Study on Different Trails in the Ecological Reserve of Guapiaçu, RJ

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Agricultural Sciences 2023年第2期14卷 196-201页

作者： Johnatan Jair de Paula Marchiori Vinicius de souza Oliveira Gilcéa Teixeira Fontana Boone Luan Luciano dos Santos Pereira Natália cristina Barbosa Moura Ana Beatriz Mamedes Piffer Anderson Mathias Holtz Lusiane de Sousa Ferreira Raquel cetto Sampaio Federal Rural University of Rio de Janeiro Seropédica Brazil center for Agricultural Sciences and Engineering Federal University of Espírito Santo Alegre Brazil campus Itapina Federal Institute of Espírito Santo Colatina Brazil State University Paulista “Júlio de Mesquita filho” Botucatu Brazil

Bromeliaceae are important in ecological restoration, showing easy adaptation to different environments, and constantly interacting with each other through ecological relationships, whose effects may or may not benefit the species involved. The hypothesis tested whether the population density of Hohenbergia augusta is higher in areas at a more advanced successional stage (brown trail), since the species is typical of more structured forest environments, occurring especially in the lower strata of the forest. Six plots of 10 meters (6 × 10 m) were installed, parallel to the brown and yellow trails, and with a distance of 5 m from the edge. Plots were allocated alternately between the two sides of the trail, 10 meters apart from each other when on the same side. In each plot, all H. augusta (Vell.) E. Morren bromeliads were sampled, without a minimum inclusion criterion, provided they were epiphytic phorophytes. The logarithm of the value was used for data normalization and later, the data were tested in a student’s t-test. The study pointed out that the highest population density of H. augusta occurred in the brown trail because the area is in a more advanced successional stage.

关键词： Bromeliads Atlantic Forest Ecological Restoration

Osmoregulators in Hymenaea courbaril and Hymenaea stigonocarpa under water stress and rehydration

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Journal of Forestry Research 2018年第6期29卷 1475-1479页

作者： Luma castro de souza Luana Moraes da Luz Je′ssica Taynara da Silva Martins candido Ferreira de Oliveira Neto Juscelino Goncalves Palheta Tamires Borges de Oliveira Ediane conceicao Alves Risely Ferraz de Almeida Raimundo Leonardo Lima de Oliveira Roberto cezar Lobo da costa Nariane Quaresma Vilhena Universidade estadual paulista ju′lio de mesquita filho JaboticabalSP 14884-900Brazil Universidade Federal Rural da Amazonia(UFRA) Campus CapanemaCapanema 68700-030Brazil Universidade Federal Rural da Amazonia Campus BelemBelem 66077-830Brazil Universidade Federal de Vicosa(UFV) Vicosa 36570-900Brazil Universidade Estadual Paulista Ju′lio de Mesquita Fiho(UNESP) Jaboticabal 14884-900Brazil Universidade do Estado do Para′(UEPA) Bele′m 66113-200Brazil

The objective of this work was to evaluate the effect of different water deficiency and rehydration levels on the concentrations of osmoregulators in two plant species（Hymenaea courbaril and H. Stigonocarpa） in the Amazon. We adopted a 2×5×5 factorial system,referring to 2 species（H. courbaril and H. stigonocarpa）and 5 stages of hydration and rehydration. The five hydration and rehydration stages were established in:（1）control treatment E0;（2） Plants with 13 days of stress after incubation—E13;（3） Plants with 26 days of stress E26;（4）The plants that were established after 26 days after incubation and rehydrated for two days（RD2）;（5） rehydrated for two days（RD4）. The plants that were established after 26 days after incubation and rehydrated for four days. The experiment totaled fifty young plants with five *** measurements were performed at the beginning of the experiment（E0） at 13（E13） and 26（E26） days after the water stress, in which the plants were rehydrated,repeating the analyses after two（RD2） and four（RD4）days. Both species increased the sucrose concentration by18%, with a decrease of 52% in starch content. The RD4 time presented the highest mean starch concentration(0.19 mmol g-1 of the residue for H. courbaril and0.27 mmol g;of residue for H. stigonocarpa). Increased proline concentrations were recorded for controls until RD2 for both species. For glycine betaine, the highest increases in treatments E26 and RD2 were observed for the H. courbaril species. Our rehydration period was not sufficient for total recovery of pre-stress concentrations of all studied solutes.

关键词： Amazonia Water stress Osmotic adjustment Proline Sucrose

Levels of Trihalomethanes in Stored Water from High and Fundamental Schools:comparison between Two Temporal Data Sets

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Journal of Water Resource and Protection 2018年第6期10卷 577-586页

作者： Ricardo Andreola Anny Rosi Mannigel Graciene de souza Bido Thaise Moser Teixeira Edison Schmidt filho Jussara Ricardo de Oliveira Andre Ribeiro da costa Rebecca Manesco Paixao centro Universitário de Maringá(UNIcESUMAR) MaringáBrazil Universidade Estadual de Maringá(UEM) MaringáBrazil

Epidemiological studies have been investigating the relationship between chlorination byproducts exposure and cancer. Studies showed the incidence of colon, rectum and bladder cancer in laboratory animals when halogenated byproducts were administered to them, such as trihalomethanes. Based on this fact, in this work, two data sets of water quality parameters were analyzed with focus on total trihalomethanes (THMt). These two data sets are from two different time periods (one in 2014 and other in 2017). All the samples were collected in the same months, in both data sets. The samples were taken from its same corresponding sampling points in both periods of time. Trihalomethanes (THMs) are undesired byproducts of chlorination and its formation occurs when the chlorine used in water treatment reacts with natural organic matter, which is present in natural waters, during the disinfection process. The aim of this research was to investigate the THMs levels in storage water from the chlorination performed by the Water Treatment Station (WTS) of the Maringá-Parana-Brazil;also, to compare the results obtained with the maximum allowable values (MAVs) established by the consolidation Resolution n.05/2017, current law of water quality in Brazil. Water samples were collected in eight high and fundamental schools of Maringá-Paraná-Brazil and analyzed through the gas chromatography method by the use of mass spectrometry detector with purge-and-trap concentrator (Gc-MS) for THM. Furthermore, parameters such as pH and residual chlorine were analyzed following the methodology proposed by the Standard Methods for the Examination of Water and Wastewater. The study results to THMt show that the maximum value of 21.5 μg/L obtained is within the MAV of 100 μg/L. chloroform was the compound with higher concentrations in all samples in THMt analysis. Results of residual chlorine and pH also are within the MAVs. Studies like this are important to continuous monitoring of the water quality d

关键词： Water Treatment chlorination Byproducts cancer Tap Water

Study of the roles of caspase-3 and nuclear factor kappa B in myenteric neurons in a P2X7 receptor knockout mouse model of ulcerative colitis

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World Journal of Gastroenterology 2023年第22期29卷 3440-3468页

作者： Henrique Inhauser Riceti Magalhães Felipe Alexandre Machado Roberta Figueiroa souza Marcos Antônio Ferreira caetano Vanessa Ribeiro Figliuolo Robson coutinho-Silva Patricia castelucci department of Surgery School of Veterinary Medicine and Animal SciencesUniversity of São PauloSão Paulo 05508-270Brazil department of Anatomy University of São PauloSão Paulo 05508-000Brazil Biophysics Institute carlos chagas filho Federal University of Rio de JaneiroRio de Janeiro 21941-902Brazil

BAcKGROUND The literature indicates that the enteric nervous system is affected in inflammatory bowel diseases(IBDs)and that the P2X7 receptor triggers neuronal ***,the mechanism by which enteric neurons are lost in IBDs is *** To study the role of the caspase-3 and nuclear factor kappa B(NF-κB)pathways in myenteric neurons in a P2X7 receptor knockout(KO)mouse model of *** Forty male wild-type(WT)c57BL/6 and P2X7 receptor KO mice were euthanized 24 h or 4 d after colitis induction by 2,4,6-trinitrobenzene sulfonic acid(colitis group).Mice in the sham groups were injected with *** mice were divided into eight groups(n=5):The WT sham 24 h and 4 d groups,the WT colitis 24 h and 4 d groups,the KO sham 24 h and 4 d groups,and the KO colitis 24 h and 4 d *** disease activity index(DAI)was analyzed,the distal colon was collected for immunohistochemistry analyses,and immunofluorescence was performed to identify neurons immunoreactive(ir)for calretinin,P2X7 receptor,cleaved caspase-3,total caspase-3,phospho-NF-κB,and total NF-κ*** analyzed the number of calretinin-ir and P2X7 receptor-ir neurons per ganglion,the neuronal profile area(μm^(2)),and corrected total cell fluorescence(cTcF).RESULTS cells double labeled for calretinin and P2X7 receptor,cleaved caspase-3,total caspase-3,phospho-NF-κB,or total NF-κB were observed in the WT colitis 24 h and 4 d *** number of calretinin-ir neurons per ganglion was decreased in the WT colitis 24 h and 4 d groups compared to the WT sham 24 h and 4 d groups,respectively(2.10±0.13 vs 3.33±0.17,Pcantly different between the KO *** calretinin-ir neuronal profile area was increased in the WT colitis 24 h group compared to the WT sham 24 h group(312.60±7.85 vs 278.41±6.65,Pclear profile area was decreased in the WT colitis 4 d group compared to the WT sham 4 d group(104.63±2.49 vs 117.41±1.14,P<0.01).The number of

关键词： cell death Enteric nervous system Gastroenterology Inflammatory bowel diseases P2X7 receptor Purinergic signaling

Effect of Growth Regulators in Production and Rooting of coffea arabica ***

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American Journal of Plant Sciences 2018年第4期9卷 628-636页

作者： Paulo Fernando Marques cavalcanti filho Diego corona Baitelle Sílvio de Jesus Freitas Waldinei souza da Silva Paulo cesar dos Santos Weverton Pereira Rodrigues Silverio de Paiva Freitas Ismael Lourenco de Jesus Freitas Tamara Locatelli Abraao carlos Verdin filho Guilherme Bessa Miranda department of Plant Science State University of Northern Rio de JaneiroCampos dos GoitacazesRio de Janeiro StateBrazil

The plants of c. arabica do not naturally produce a large number of orthotropic sprouts, hindering clonal multiplication by cutting, which would be the simplest technique with a lower cost of production compared to other methods of vegetative propagation. An alternative form, used successfully in the propagation of eucalyptus, is propagation by minicutting, which consists of pruning the shoot apex of the plant, forming the ministumps, which in variable time interval emits the shoots that will be used to make minicutting. In c. arabica plants the number of shoots can be increased with the application of growth regulators and fertilizers. So, the purpose of this work was to verify the effect of growth regulators and foliar fertilizers on the emission and development of orthotropic shoots of c. arabica. The experiment was installed in a randomized complete block design, with ten treatments composed by growth regulators and fertilizers: T1 (control);T2 (Stimulate + Sturdy + Enervig);T3 (Stimulate + Vitakelp + Biozyme);T4 (Tiba + Sturdy + Enervig);T5 (Tiba + Vitakelp + Biozyme);T6 (Brs + Sturdy + Enervig) T7 (Brs + Vitakelp + Biozyme);T8 (Stimulate);T9 (TIBA);T10 (Brs-Brassinoesteroid), each plot consisting of two plants. Biometric and physiological parameters were evaluated for the ministumps, as well as the biometric parameters of the shoots and plant survival. The results showed that there was no influence of the treatments on the biometric parameters of the ministumps. A greater number of orthotopic sprouts were obtained in treatments with the TIBA growth regulator, as well as a greater plant survival of the minicuttings, after 25 days of transplanting in a greenhouse. It was concluded that TIBA treatments influenced the production and quality of clonal minicuttings of c. arabica.

关键词： Arabica coffee Ministumps clonal Propagation

Serum 1,3-beta-D-glucan as a noninvasive test to predict histologic activity in patients with inflammatory bowel disease

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World Journal of Gastroenterology 2021年第9期27卷 866-885页

作者： Katia Farias e Silva Hayandra F Nanini cynthia Machado cascabulho Siane L B Rosas Patricia T Santana Antonio Joséde V carneiro Elias Anaissie Marcio Nucci Heitor Siffert Pereira de souza department of clinical Medicine School of MedicineFederal University of Rio de JaneiroRio de Janeiro 21941-913Brazil Laboratory of Innovations in Therapies Education and BioproductsInstituto Oswaldo CruzRio de Janeiro 21040-360Brazil clinical Trial and consulting Services CincinnatiOH 45267United States Internal Medicine D'Or Institute for Research and Education(IDOR)Rio de Janeiro 22281-100Brazil

BAcKGROUND 1,3-beta-D-glucan(BG)is a ubiquitous cell wall component of gut *** hypothesized that the serum levels of BG could reflect active intestinal inflammation in patients with inflammatory bowel *** To determine whether the serum BG concentrations correlate with intestinal *** A prospective observational study was performed in a tertiary referral center,from 2016 to 2019,in which serum BG was determined in 115 patients with crohn’s disease(cD),51 with ulcerative colitis(Uc),and 82 controls using a photometric detection *** activity was determined by ileocolonoscopy,histopathology,magnetic resonance enterography,and biomarkers,including fecal calprotectin(Fc),c-reactive protein,and a panel of *** ability of BG to detect active vs inactive disease was assessed using the area under the receiver operating characteristic *** subgroup analysis,serial BG was used to assess the response to therapeutic *** The serum BG levels were higher in cD patients than in controls(P=0.0001).The BG levels paralleled the endoscopic activity in cD patients and histologic activity and combined endoscopic and histologic activity in both cD and Uc *** area under the curve(AUc)in receiver operating characteristic analysis to predict endoscopic activity was 0.694[95%confidence interval(cI):0.60-0.79;P=0.001]in cD,and 0.662(95%cI:0.51-0.81;P=0.066)in Uc *** AUc in receiver operating characteristic analysis to predict histologic activity was 0.860(95%cI:0.77-0.95;PcD,and 0.786(95%cI:0.57-0.99;P=0.015)in Uc *** cut-off values of BG for both endoscopic and histologic activity were 60μg/mL in cD,and 40μg/mL in Uc *** analysis showed that the results based on BG of 40 and 60μg/mL were more specific for predicting endoscopic activity(71.8%and 87.2%for cD;and 87.5%and 87.5%for Uc,respectively)than Fc(53.3%and 66.7%for cD;and 20%and 80%for Uc,respectively);and

关键词： crohn’s disease Ulcerative colitis Inflammatory bowel disease Beta-glucan Histologic activity Noninvasive test

deep sedation during gastrointestinal endoscopy: Propofol-fentanyl and midazolam-fentanyl regimens

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World Journal of Gastroenterology 2013年第22期19卷 3439-3446页

作者： Marcos Eduardo Lera dos Santos Fauze Maluf-filho Dalton Marques chaves Sergio Eiji Matuguma Edson Ide Gustavo de Oliveira Luz Thiago Ferreira de souza Fernanda c Simoes Pessorrusso Eduardo Guimares Hourneaux de Moura Paulo Sakai department of Gastroenterology Hospital das Clínicas University of Sao Paulo Medical School Sao Paulo CEP 05403-900 Brazil cancer Institute of Sao Paulo State Sao Paulo CEP 01246-000 Brazil

AIM: To compare deep sedation with propofol-fentanyl and midazolam-fentanyl regimens during upper gastrointestinal endoscopy. METHODS: After obtaining approval of the research ethics committee and informed consent, 200 patients were evaluated and referred for upper gastrointestinal endoscopy. Patients were randomized to receive propofol-fentanyl or midazolam-fentanyl (n = 100/group).We assessed the level of sedation using the observer's assessment of alertness/sedation (OAA/S) score and bispectral index (BIS). We evaluated patient and physician satisfaction, as well as the recovery time and complication rates. The statistical analysis was performed using SPSS statistical software and included the MannWhitney test, χ 2 test, measurement of analysis of variance, and the κ statistic. RESULTS: The times to induction of sedation, recovery, and discharge were shorter in the propofolfentanyl group than the midazolam-fentanyl group. According to the OAA/S score, deep sedation events occurred in 25% of the propofol-fentanyl group and 11% of the midazolam-fentanyl group (P = 0.014). Additionally, deep sedation events occurred in 19% of the propofol-fentanyl group and 7% of the midazolamfentanyl group according to the BIS scale (P = 0.039). There was good concordance between the OAA/S score and BIS for both groups (κ = 0.71 and κ = 0.63, respectively). Oxygen supplementation was required in 42% of the propofol-fentanyl group and 26% of the midazolam-fentanyl group (P = 0.025). The mean time to recovery was 28.82 and 44.13 min in the propofolfentanyl and midazolam-fentanyl groups, respectively (P complications in either group. Although patients were equally satisfied with both drug combinations, physicians were more satisfied with the propofol-fentanyl combination. cONcLUSION: deep sedation occurred with propofolfentanyl and midazolam-fentanyl, but was more frequent in the former. Recovery was faster in the propofol-fentanyl group.

关键词： Endoscopy deep sedation Anesthetic administration Anesthetic dose Adverse effects

Effects of climate and Agricultural Practices on Temporal Progress of Sclerotinia sclerotiorum for Soybean in Brazil

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Agricultural Sciences 2016年第7期7卷 425-438页

作者： Gustavo castilho Beruski André Belmont Pereira David souza Jaccoud-filho Felipe Fadel sartori Paulo cesar Sentelhas department of Biosystems Engineering University of Sao Paulo—ESALQ/USP Piracicaba Brazil department of Soil Science and Agricultural Engineering State University of Ponta Grossa Ponta Grossa Brazil department of Plant Sciences and crop Protection State University of Ponta Grossa Ponta Grossa Brazil

currently the soybean crop is affected by the white mold (Sclerotinia sclerotiorum). The disease can reduce the crop yield and quality and decrease the prices of agricultural lands. The aim of the current research was to assess epidemiology of white mold on soybean crop grown at Arapoti, PR, Brazil, utilizing data related to agricultural practices and local meteorological factors. The experiment was conducted in a naturally infested area. The soybean crop was sown on October 18th, 2011. The experimental design adopted herein was a randomized block in a factorial combination with 4 row spacings (0.35, 0.45, 0.60, 0.75 m) and 4 plant populations (150, 200, 250, 300 thousand plants per hectare) and 4 replications. The temporal analysis of the epidemic was evaluated using mathematical models, such as Logistics, Monomolecular and Gompertz, in order to determine the best model that described the progress of the disease as a function of local meteorological elements. For the incidence data it has been shown that both logistic and monomolecular models were those that were best fitted to the experimental data. For severity, the best model related to the experimental data was the logistic one. Either for incidence or for severity, air temperature was considered to be the environmental factor most affecting the progress of the disease. The variability in the apparent infection rates of white mold on soybean was not affected by different row spacings and plant populations;therefore, suggesting that macroclimatic variations prevailed in such a fashion to mitigate the effect of cultural practices adopted in the field.

关键词： Meteorological Data Sclerotinia sclerotiorum (Lib.) de Bary Glycine max Row Spacing Plant Population Epidemiology

Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis

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World Journal of Gastroenterology 2012年第32期18卷 4278-4287页

作者： Rodrigo Goulart Pacheco christiano costa Esposito Lucas cM Müller Morgana TL castelo-Branco Leonardo Pereira Quintella Vera Lucia A chagas Heitor Siffert P de souza Alberto Schanaider department of Surgery Experimental Surgery CenterSurgical Sciences Postgraduate ProgramMedical SchoolFederal University of Rio de JaneiroRio de JaneiroRJ 21941-913Brazil Laboratory of cellular Immunology Department of Histology and EmbryologyInstitute of Biomedical SciencesFederal University of Rio de JaneiroRio de JaneiroRJ 21941-913Brazil department of Pathology Medical SchoolFederal University of Rio de JaneiroRio de JaneiroRJ 21941-913Brazil department of Internal Medicine Hospital Universitario Clementino Fraga FilhoFederal University of Rio de JaneiroRio de JaneiroRJ 21941-913Brazil

AIM:To investigate whether butyrate or glutamine enemas could diminish inflammation in experimental diversion ***:Wistar specific pathogen-free rats were submitted to a Hartmann's end colostomy and treated with enemas containing glutamine,butyrate,or *** were administered twice a week in the excluded segment of the colon from 4 to 12 wk after the surgical ***-up colonoscopy was performed every 4 wk for 12 *** effect of treatment was evaluated using video-endoscopic and histologic scores and measuring interleukin-1β,tumor necrosis factor-alpha,and transforming growth factor beta production in organ cultures by enzyme linked immunosorbent ***:colonoscopies of the diverted segment showed mucosa with hyperemia,increased number of vessels,bleeding and mucus *** with either glutamine or butyrate induced significant reductions in both colonoscopic(P cal scores(P densities of collagen fibers in tissue(P = 0.015;P = 0.001),the number of goblet cells(P = 0.021;P = 0.029),and the rate of apoptosis within the epithelium(P = 0.043;P = 0.011) to normal *** high levels of cytokines in colon explants from rats with diversion colitis significantly decreased to normal values after treatment with butyrate or ***:The improvement of experimental diversion colitis following glutamine or butyrate enemas highlights the importance of specific luminal nutrients in the homeostasis of the colonic mucosa and supports their utilization for the treatment of human diversion colitis.

关键词：结肠炎丁酸引水实验炎症酶联免疫吸附试验纤维化谷氨酸