Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis

作     者：Rodrigo Goulart Pacheco Christiano Costa Esposito Lucas CM Müller Morgana TL Castelo-Branco Leonardo Pereira Quintella Vera Lucia A Chagas Heitor Siffert P de Souza Alberto Schanaider 

作者机构：Department of SurgeryExperimental Surgery CenterSurgical Sciences Postgraduate ProgramMedical SchoolFederal University of Rio de JaneiroRio de JaneiroRJ 21941-913Brazil Laboratory of Cellular ImmunologyDepartment of Histology and EmbryologyInstitute of Biomedical SciencesFederal University of Rio de JaneiroRio de JaneiroRJ 21941-913Brazil Department of PathologyMedical SchoolFederal University of Rio de JaneiroRio de JaneiroRJ 21941-913Brazil Department of Internal MedicineHospital Universitario Clementino Fraga FilhoFederal University of Rio de JaneiroRio de JaneiroRJ 21941-913Brazil 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2012年第18卷第32期

页      面：4278-4287页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by Grants from the Brazilian Research Council Fundao de Amparo à Pesquisa do Estado do Rio de Janeiro 

主　　题：Diversion colitis Butyrate Glutamine Shortchain fatty acids Cytokines 

摘      要：AIM:To investigate whether butyrate or glutamine enemas could diminish inflammation in experimental diversion ***:Wistar specific pathogen-free rats were submitted to a Hartmann s end colostomy and treated with enemas containing glutamine,butyrate,or *** were administered twice a week in the excluded segment of the colon from 4 to 12 wk after the surgical ***-up colonoscopy was performed every 4 wk for 12 *** effect of treatment was evaluated using video-endoscopic and histologic scores and measuring interleukin-1β,tumor necrosis factor-alpha,and transforming growth factor beta production in organ cultures by enzyme linked immunosorbent ***:Colonoscopies of the diverted segment showed mucosa with hyperemia,increased number of vessels,bleeding and mucus *** with either glutamine or butyrate induced significant reductions in both colonoscopic(P 0.02) and histological scores(P 0.01) and restored the densities of collagen fibers in tissue(P = 0.015;P = 0.001),the number of goblet cells(P = 0.021;P = 0.029),and the rate of apoptosis within the epithelium(P = 0.043;P = 0.011) to normal *** high levels of cytokines in colon explants from rats with diversion colitis significantly decreased to normal values after treatment with butyrate or ***:The improvement of experimental diversion colitis following glutamine or butyrate enemas highlights the importance of specific luminal nutrients in the homeostasis of the colonic mucosa and supports their utilization for the treatment of human diversion colitis.