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Trichomonas vaginalis perturbs the junctional complex in epithelial cells

cell Research 2005年第9期 29-41页

作者： Rodrigo Furtado MADEIRO da cOSTA,Wanderley de SOUZA,marlene BENcHIMOL,John F ALDERETE,José Andrés MORGADO-DíAZ Programa de Pós Graduao em ciências Morfológicas Universidade Federal do Rio de Janeiro Rio de Janeiro RJ 20231- 050 Brazil Grupo de Biologia Estrutural Diviso de Biologia celular centro de Pesquisa Instituto Nacional de cancer Rio de Janeiro RJ 20231-050 Brazil Grupo de Biologia Estrutural Diviso de Biologia celular centro de Pesquisa Instituto Nacional de cancer Rio de Janeiro RJ 20231-050 Brazil Laboratório de Ultraestrutura celular Hertha Meyer Instituto de Biofísica carlos chagas Filho Universidade Federal do Rio de Janeiro centro de ciências da Saúde Rio de Janeiro RJ 21944-900 Brazil Laboratório de Ultraestrutura celular Universidade Santa úrsula Rio de Janeiro RJ 22231-010 Brazil Department of Microbiology University of Texas Health Science center San Antonio TX 78229-3900 USA Grupo de Biologia Estrutural Diviso de Biologia celular centro de Pesquisa Instituto Nacional de cancer Rio de Janeiro RJ 20231-050 Brazil

Trichomonas vaginalis, a protist parasite of the urogenital tract in humans, is the causative agent of trichomonosis, which in recent years have been associated with the cervical cancer development. In the present study we analyzed the modifications at the junctional complex level of caco-2 cells after interaction with two isolates of T. vaginalis and the influence of the iron concentration present in the parasite’s culture medium on the interaction effects. Our results show that T. vaginalis adheres to the epithelial cell causing alterations in the junctional complex, such as: (a) a decrease in transepithelial electrical resistance; (b) alteration in the pattern of junctional complex proteins distribution as observed for E-cadherin, occludin and ZO-1; and (c) enlargement of the spaces between epithelial cells. These effects were dependent on (a) the degree of the parasite virulence isolate, (b) the iron concentration in the culture medium, and (c) the expression of adhesin proteins on the parasite surface.

关键词： Trichomonas vaginalis caco-2 junctional complex proteins transepithelial electrical resistance adhesins.

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Germination of Encholirium spectabile *** Schult.&*** in Response to Temperature and Water Stress

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American Journal of Plant Sciences 2014年第15期5卷 2237-2246页

作者： marlene Feliciano Figueiredo Francisco carlos Barboza Nogueira charles Lobo Pinheiro Selma Freire de Brito Sebastiao Medeiros Filho University of Vale do Acarau—UVA Campus BetaniaCity of SobralBrazil Seed Analysis Laboratory Department of PhytotecnicFederal University of Ceara—UFCCampus do PiciCity of FortalezaBrazil

A study was conducted to examine the effects of different temperatures and water stress on Encholirium spectabile seeds. We evaluated the germination percentage, speed and time of germination of small (4.01 mm), at temperatures of 15°c, 20°c, 25°c, 30°c, 35°c, 40°c and 45°c and a photoperiod of 12 hours of light. Seeds were subjected to osmotic potentials of 0, -0.2, -0.4, -0.6, -0.8, -1.0, -1.2 and -1.4 MPa, induced by solutions of polyethylene glycol. The results indicated an excellent germination of medium and large seeds at the temperatures of 20°c, 25°c, 30°c and 35°c. The temperature of 35°c provided the best mean germination time of large seeds and a higher speed rate of medium and large seeds. E. spectabile seeds germinate in a wide range of water deficit of -0.2 to -1.2 MPa. Germination reduced at concentrations of -1.0 and -1.2 MPa. No germination occurred at -1.4 MPa. Larger seeds showed the higher germination potential than medium and small seeds at the temperatures of 25°c, 30°c and 35°c and in the range ofwater deficit of -0.2 to -1.2 Mpa.

关键词： Seeds Size Native Species Osmotic Potential Polyethylene Glycol Semiarid Region

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治疗用单克隆抗体的超敏反应:表型和内型

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中华临床免疫和变态反应杂志 2018年第5期12卷 583-596页

作者： Ghislaine Annie c.Isabwe marlene Garcia Neuer Leticia de las Vecillas Sanchez Donna-Marie Lynch FNP-Bc,Kathleen Marquis Mariana castells 顾建青(翻译) 王良录(审校) 不详中国医学科学院、北京协和医学院、北京协和医院变态(过敏)反应科

背景单克隆抗体的广泛的应用导致了超敏反应(HSRs)发生率的增加,这阻碍了单克隆抗体作为一线治疗方法在临床上的应用。HRSs的症状、诊断方法及定向治疗方法均尚未标准化。目的我们基于临床表型、潜在的内型和生物标志物进行脱敏的治疗... 详细信息

背景单克隆抗体的广泛的应用导致了超敏反应(HSRs)发生率的增加,这阻碍了单克隆抗体作为一线治疗方法在临床上的应用。HRSs的症状、诊断方法及定向治疗方法均尚未标准化。目的我们基于临床表型、潜在的内型和生物标志物进行脱敏的治疗方法提出一种新的基于循证医学的m Ab HSR分类方法。方法描述了104例患者对16种m Abs HSR的表型、内型和生物标志物,并与526次皮下及静脉脱敏治疗结果进行比较。结果初始反应呈现4种模式:Ⅰ型样反应占63%,细胞因子释放反应占13%,混合反应占21%,Ⅳ型迟发反应占3%。与之形成鲜明对比的是,在脱敏治疗期间突发的HSRs为23%,其中52%为细胞因子释放反应,32%为Ⅰ型反应,12%为混合型反应,4%为Ⅰ型伴Ⅳ型迟发超敏反应。58例患者对10种m Abs进行皮肤试验,41%的患者出现阳性。在脱敏治疗期间,1例患者血清类胰蛋白酶水平升高,8例患者IL-6水平升高,均为细胞因子释放表型。结论 m Abs引起的HSRs可界定为Ⅰ型、细胞因子释放型、混合(Ⅰ型/细胞因子释放型)型和Ⅳ型反应,可通过生物标志物如皮肤试验、类胰蛋白酶和IL-6进行鉴别分型。当确诊为m Abs HSR时,这些表型有助于患者接受个性化和精准医学治疗,并为脱敏治疗的实施提供依据。脱敏治疗是一种安全有效的治疗手段,可使出现过变态反应的m Abs仍能作为一线治疗方法。

关键词：单克隆抗体超敏反应表型内型生物标志物类胰蛋白酶白细胞介素-6 皮肤试验脱敏疗法精准医学

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WH2(WASP Homolog 2)Motif Dependent Elongated F-actin Bundle Formation Induced by Missing in Metastasis(MIM) Protein

WH2(WASP Homolog 2)Motif Dependent Elongated F-actin Bundle ...

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中国细胞生物学学会第九次会员代表大会暨青年学术大会

作者： Ying Wang~1 Kang Zhou~2 Xi Zhan~2 2 University of Maryland marlene Stewart Greenebaum cancer center,center for Vascular and Inflammatory Diseases,MD 21201,USA 湖南师范大学生命科学学院

MIM 作为新的细胞骨架蛋白,具有很强的形态学功能。其 N 末端 IMD(IRSp53 and MIM shared domain) 区域具有纤维肌动蛋白(F-actin)结合以及纤维肌动蛋白束(F-actin bundle)形成功能,而 c 末端 WH2为球形肌动蛋白(G-actin)结合区域。在... 详细信息

MIM 作为新的细胞骨架蛋白,具有很强的形态学功能。其 N 末端 IMD(IRSp53 and MIM shared domain) 区域具有纤维肌动蛋白(F-actin)结合以及纤维肌动蛋白束(F-actin bundle)形成功能,而 c 末端 WH2为球形肌动蛋白(G-actin)结合区域。在细胞系中过量表达 MIM 诱导多种细胞形态,其中 LNcaP 中全长 MIM 蛋白

关键词： WH2 missing in metastasis F-actin bundle

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Biofabrication and biomanufacturing in Ireland and the UK

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Bio-Design and Manufacturing 2024年第6期7卷 825-856页

作者： Jack F.Murphy Martha Lavelle Lisa Asciak Ross Burdis Hannah J.Levis cosimo Ligorio Jamie Mc Guire marlene Polleres Poppy O.Smith Lucinda Tullie Juan Uribe-Gomez Biqiong chen Jonathan I.Dawson Julien E.Gautrot Nigel M.Hooper Daniel J.Kelly Vivian S.W.Li Alvaro Mata Abhay Pandit James B.Phillips Wenmiao Shu Molly M.Stevens Rachel L.Williams James P.K.Armstrong Yan Yan Shery Huang Department of Engineering University of CambridgeCambridge CB21PZUK Department of Translational Health Sciences Bristol Medical SchoolUniversity of BristolBristol BS13NYUK Department of Biomedical Engineering University of StrathclydeGlasgow G40NWUK Department of Materials Institute of Biomedical EngineeringImperial College LondonLondon SW72AZUK Department of Bioengineering Institute of Biomedical EngineeringImperial College LondonLondon SW72AZUK Department of Eye and Vision Science Institute of Life Course and Medical SciencesUniversity of LiverpoolLiverpool L78TXUK Biodiscovery Institute University of NottinghamNottingham NG72RDUK Department of chemical and Environmental Engineering University of NottinghamNottingham NG72RDUK centre for Human Development Stem Cells and RegenerationHuman Development and HealthFaculty of MedicineUniversity of SouthamptonSouthampton S0166YDUK Division of Neuroscience School of Biological SciencesFaculty of BiologyMedicine and HealthUniversity of ManchesterManchester M139PTUK UcL centre for Nerve Engineering Department of PharmacologyUCL School of PharmacyUniversity College LondonLondon WC1N 1AXUK Stem cell and cancer Biology Laboratory The Francis Crick InstituteLondon NW11ATUK cÚRAM SFI Research Centre for Medical DevicesUniversity of GalwayGalway H91 W2TYIreland School of Mechanical and Aerospace Engineering Queen’s University BelfastBelfast BT95AHUK School of Engineering and Materials Science Queen Mary University of LondonLondon E14NSUK Geoffrey Jefferson Brain Research centre Manchester Academic Health Science CentreNorthern Care Alliance and University of ManchesterManchester M139PLUK Trinity centre for Biomedical Engineering Trinity Biomedical Sciences InstituteTrinity College DublinDublin 2Ireland Department of Mechanical Manufacturing and Biomedical EngineeringSchool of EngineeringTrinity College DublinDublin 2Ireland Advanced Materials and Bioengineering Research centre(AMBER) Royal College of Surgeons in Ireland and T

As we navigate the transition from the Fourth to the Fifth Industrial Revolution,the emerging fields of biomanufacturing and biofabrication are transforming life sciences and *** sectors are benefiting from a synergy of synthetic and engineering biology,sustainable manufacturing,and integrated design *** techniques such as 3D bioprinting,tissue engineering,directed assembly,and self-assembly are instrumental in creating biomimetic scaffolds,tissues,organoids,medical devices,and biohybrid *** field of biofabrication in the United Kingdom and Ireland is emerging as a pivotal force in bioscience and healthcare,propelled by cutting-edge research and *** on the production of biologically functional products for use in drug delivery,in vitro models,and tissue engineering,research institutions across these regions are dedicated to innovating healthcare solutions that adhere to ethical standards while prioritising sustainability,affordability,and healthcare system benefits.

关键词： Bioprinting Drug delivery Biomaterials Tissue engineering Sustainability Biohybrid

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cancer and covid-19 in the Department of Internal Medicine and Medical Oncology at the National Teaching Hospital Hubert Koutoukou Maga of cotonou, about 4 cases: A Review of the Literature

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Open Journal of Internal Medicine 2021年第4期11卷 298-309页

作者： Azon Kouanou Angèle Agbodandé Kouessi Anthelme Sokadjo Yves Morel Missiho Mahoutin Semassa Ghislain Faladé Adélakoun Ange Géoffroy Murhula Katabana Delphin Kouanou Olamidé marlene Marie Dénise Ahoussoussi cornelly Tokpanoudé Elodie Zannou Djimon Marcel Internal Medicine-Medical Oncology Unity of the National Teaching Hospital center (NTHc)-Hubert Kougougou Maga (HKM) of cotonou Cotonou Benin Internal Medicine Department University Clinics Bukavu (U.O.B) Democratic Republic of the Congo

coronavirus Disease-19 (cOVID-19) manifests itself by a respiratory attack that can go from mild to severe forms. The factors favoring the severe forms are age, arterial hypertension, diabetes mellitus, and cancer. We report 4 cases of cOVID-19 on cancer, followed in the Department of Internal Medicine and Medical Oncology of National Teaching Hospital center Hubert Kougougou Maga (NTHc-HKM). case 1: A hypertensive 65-year-old female patient with a severe form of cOVID-19 on an FIGO (International Federation of Gynecology and Obstetrics) IVB endometrioid adenocarcinoma with pulmonary metastasis: unfavorable evolution under treatment with death. case 2: A 56-year-old hypertensive patient with severe cOVID-19 with pulmonary metastatic recurrence of SBRIII, HER2 positive invasive ductal carcinoma of the left breast: favorable evolution with cure of cOVID-19, subsequent death related to active tumor. case 3: A 37-year-old patient with a moderate form of cOVID-19 with hepatocellular carcinoma complicating post alcoholic cirrhosis, cHILD c: favorable evolution with cure of cOVID-19, patient lost to follow-up. case 4: A 56-year-old patient, hypertensive, type II diabetic, presenting a severe form of cOVID-19 on a field of ovarian serous adenocarcinoma of high grade FIGO IV A with pleuropulmonary metastasis: favorable evolution with cure of cOVID-19, subsequent stroke, patient followed in palliative home hospitalization. The diagnosis of cOVID-19 in cancer with pulmonary metastases is difficult. The pulmonary location of tumors seems to be a factor favoring severe forms.

关键词： cOVID-19 cancer NTHc-HKM

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极高强度他汀治疗对冠状动脉粥样硬化消退的影响——ASTEROID试验

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美国医学会杂志（中文版） 2006年第4期25卷 215-223页

作者： Steven E. Nissen Stephen J. Nicholls Ilks Sipahi Peter Libby Joel S. Raichlen christie M. Ballantyne Jean Davignon Raimund Erbel Jean charles Fruchart Jean-claude Tardif Paul Schoenhagen Tim crowe Valerie cain Kathy Wolski marlene coormastic E. Murat Tuzcu 仝其广（译）王淑敏（译）胡大一（校） Departments of cardiovascular Medicine Diagnostic Radiology cleveland clinic Lerner School of Medicine Cleveland Ohio Donald W. Reynolds cardiovascular clinical Research center Department of Medicine Brigham and Women＇s Hospital and Harvard Medical School Boston Mass AstraZeneca Wilmington Del Section of Atherosclerosis and Lipoprotein Research Department of Medicine Baylor College of Medicine and Center for Cardiovascular Disease Prevention Methodist DeBakey Heart Center Houston Tex Hyperlipidemia and Atherosclerosis Research Group Clinical Research Institute of Montreal Montreal Quebec Department of cardiology Universitat Duisburg-Essen Duisburg Germany Departement de Recherche sur les Lipoproteines et I＇Atherosclerose Pasteur de Lille Inserm U545 et Faculte de Pharmacie Universite du Droit et de la Sante de Lille 2 Lille France MHI Research center Montreal Heart Institute Montreal Quebec 不详

背景：以前的血管内超声（intravascular ultrasound，IVUS）试验证实，他汀治疗可减缓或阻止动脉粥样硬化的进展，但是迄今尚无应用动脉粥样斑块体积百分比（percent atheroma volume，PAV）证实粥样硬化消退的确切证据。PAV是最严格... 详细信息

背景：以前的血管内超声（intravascular ultrasound，IVUS）试验证实，他汀治疗可减缓或阻止动脉粥样硬化的进展，但是迄今尚无应用动脉粥样斑块体积百分比（percent atheroma volume，PAV）证实粥样硬化消退的确切证据。PAV是最严格的评价病变进展和消退的IVUS测量指标。目标：评价极高强度他汀治疗是否能逆转IVUS确定的冠状动脉粥样硬化。设计和地点：于美国、加拿大、欧洲和澳大利亚53个社区和3级保健中心进行前瞻性开标盲法终点试验（A Study to Evaluate the Effect of Rosuvastatin on Intravascular Ultrasound-Derived coronary Atheroma Burden，ASTEROID）。应用马达驱动回撤IVUS评价基线和治疗24个月时冠状动脉粥样斑块负荷。每对基线和随访IVUS测量结果均进行盲法分析。病例：从2002年11月到2003年10月，507例患者有基线IVUS检查结果，并接受至少1个剂量的研究药物。在24个月后，349例患者具有可用于评估的系列IVUS检查结果。干预：所有患者均接受瑞舒伐他汀40ms／d强化治疗。主要观测指标：预先设定了两个一级疗效指标：PAV变化和基线最严重病变10min节段动脉粥样斑块体积变化。二级疗效指标为整个动脉标准化总斑块体积的变化。结果：平均（SD）LDL—c水平由基线时的130．4（34．3）ms／dL降至60．8（20．0）ms／扎，平均下降了53．2％（P〈0．001）。平均（SD）HDL-c水平从基线时的43．1（11．1）ms／dL升至49．0（12．6）ms／dL，平均增加了14．7％（P〈0．001）。整个血管PAV平均（SD）变化为-0．98％（3．15％），中位数为-0．79％（97．5％cI，-1．21％～-0．53％）（与基线比较，P〈0．001）。最严重病变10min节段斑块体积平均（SD）变化为-6．1（10．1）mm^3，中位数为-5．6mm^3（97．5％cI，-6．8～-4．0mm^3）（与基线比较，P〈0．001）。总斑块体积变化中位数降低了6．8％，平均减少了-14．7（25．7）mm^3，中位数为-12．5mm^3（95％cI，-15．1～-10．5mm^3）（与基线比较，P〈0．001）。不良事件少见，与其他他汀试验相似。结论：应用瑞舒伐他汀40ms／d进行极高强度他汀治疗可使LDL-c平均水平达到60．8ms／dL，使HDL—c增加14．7％。这导致所有3个预先设定的IVUS斑块负荷指标均显示动脉粥样硬化消退。因此，将LDL-c降至低于目前指南规定的水平，同时显著提高HDL-c，可以使冠心病患者动脉粥样硬化斑块消退。这些变化对临床预后的影响尚需进一步研究确定。

关键词：冠状动脉粥样硬化瑞舒伐他汀强化治疗斑块消退冠状动脉粥样斑块 Intravascular 试验强度 Rosuvastatin 冠心病患者

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