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BCRP基因在非小细胞肺癌组织和非癌肺组织中的表达及意义

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癌症 2001年第3期20卷 274-278页

作者：毛友生 Austin doyle Weidong Yang Yuetong Wei mark j.Krasna douglas d.Ross 中国医学科学院中国协和医科大学肿瘤医院胸外科北京100021 Greenebaum Cancer Center University of Maryland Medical School

目的：进一步研究从人类乳腺癌细胞株 (MCF-7/AdrVp)中克隆出的、对蒽环类抗癌药耐药的 BCRP基因在非癌肺组织及非小细胞肺癌组织中的表达。方法：从术后即刻获得的正常肺组织和有活力的非小细胞肺癌组织新鲜标本中及时提取细胞总 RNA... 详细信息

目的：进一步研究从人类乳腺癌细胞株 (MCF-7/AdrVp)中克隆出的、对蒽环类抗癌药耐药的 BCRP基因在非癌肺组织及非小细胞肺癌组织中的表达。方法：从术后即刻获得的正常肺组织和有活力的非小细胞肺癌组织新鲜标本中及时提取细胞总 RNA，通过 RT-PCR及 PCR法获得并扩增 BCRP基因的 cdNA产物，再经凝胶电泳分离 BCRP基因 cdNA带，然后再通过转膜技术把这些 BCRP基因 cdNA产物转移到杂交膜上，用 Southern blot杂交技术检测 BCRP基因的表达程度。结果：细胞总 RNA分别从 8个只有肺癌组织标本和 12对同时获取的肺癌组织及非癌肺组织标本中成功提取，然后用变性凝胶电泳鉴定提取 RNA的质量。其中 4例标本因术中缺血时间太长，或有坏死炎症或术前放化疗等原因，所提 RNA有降解而放弃进一步的检测。通过 RT-PCR及 PCR方法从 16例可用标本中获得并扩增 BCRP基因的 cdNA产物，再通过转膜及 Southern blot杂交技术，最终发现 BCRP基因在正常肺组织中均有不同程度的表达（ 10/10），而在非小细胞肺癌组织标本中只有一半病人的标本有不同程度的表达 (8/16)。结论： BCRP基因可能是一个在非癌肺组织中常规表达的基因，而在部分病人的肺癌组织中可能此基因有缺失或被抑制。因此。

关键词： BCRP基因多药耐药性非小细胞肺癌基因表达

Antibody mediated rejection causing dialysis dependent acute renal failure,partially rescued with Eculizumab

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实用器官移植电子杂志 2018年第4期6卷 270-272页

作者： mark j Lerman Afzal Nikaein Michael Kuperman Transplant Services at Medical City dallas Hospital Dallas 75201TexasAmerican Texas Medical Specialty Inc.Dallas 75201TexasAmerican john Hopkins University Medical Center Baltimore 21201MarylandAmerican

抗体介导排斥反应(AMR)是导致移植肾失功的主要原因。AMR的诊断依据为患者存在供者特异性抗体(dSA)、移植肾活检标本管周毛细血管C4d染色阳性以及急性排斥反应的组织学表现。目前AMR的治疗方法包括血浆置换、静脉免疫球蛋白和抗Cd20治... 详细信息

抗体介导排斥反应(AMR)是导致移植肾失功的主要原因。AMR的诊断依据为患者存在供者特异性抗体(dSA)、移植肾活检标本管周毛细血管C4d染色阳性以及急性排斥反应的组织学表现。目前AMR的治疗方法包括血浆置换、静脉免疫球蛋白和抗Cd20治疗。这些方法对严重的AMR没有效果。Eculizumab是一种人源化的抗C5单克隆抗体,能抑制补体末端的激活。最新的研究已经表明Eculizumab能够用于治疗严重的AMR或者预防AMR。本文介绍了一例应用Eculizumab治疗有效的AMR,而该病例对传统的治疗AMR方法无效。

关键词：移植肾染色阳性患者免疫球蛋白

A 24 hour naproxen dose on gastrointestinal distress and performance during cycling in the heat

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Sports Medicine and Health Science 2020年第1期2卷 19-24页

作者： dawn M.Emerson j.mark davis Stephen CL.Chen Toni M.Torres-McGehee Craig E.Pfeifer Charles C.Emerson joseph d.Bivona justin V.Stone department of Health Sportand Exercise SciencesUniversity of KansasLawrenceKansasUSA department of Exercise Science University of South CarolinaColumbiaSCUSA division of Exercise and Sport Science Bob Jones UniversityGreenvilleSouth CarolinaUSA Applied Sport and Exercise Sciences The ParkUniversity of GloucestershireCheltenham GL502RHUnited Kingdom department of Athletics University of Missouri-Kansas CityKansas CityUSA

Using a double-blind,randomized and counterbalanced,cross-over design,we assessed naproxen's effects on gastrointestinal(GI)distress and performance in eleven volunteers(6 male,5 female).Participants completed 4 trials:1)placebo and ambient);2)placebo and heat;3)naproxen and ambient;and 4)naproxen and *** variables were one placebo or 220 mg naproxen pill every 8 h(h)for 24 h and ambient(22.7±1.8℃)or thermal environment(35.7±1.3℃).Participants cycled 80 min at a steady heart rate then 10 min for maximum *** exertion was measured throughout *** distress was assessed pre-,during,post-,3 h post-,and 24 h post-cycling using a GI index for upper,lower,and systemic *** statistically significant differences occurred between conditions at any time for GI symptoms or perceived exertion,distance,or heart rate during maximum effort.A 24 h naproxen dose did not significantly affect performance or cause more frequent or serious GI distress when participants were euhydrated and cycling at moderate intensity in a thermal environment.

关键词： NSAId Gastrointestinal bleeding Heart rate Perceived exertion

The Piks allele of the NLR immune receptor Pik breaks the recognition of AvrPik effectors of rice blast fungus

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journal of Integrative Plant Biology 2023年第3期65卷 810-824页

作者： Gui Xiao Wenjuan Wang Muxing Liu Ya Li jianbin Liu Marina Franceschetti Zhaofeng Yi Xiaoyuan Zhu Zhengguang Zhang Guodong Lu mark j.Banfield jun Wu Bo Zhou State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research CenterChangsha 410128China International Rice Research Institute Metro Manila 1301Philppines Guangdong Provincial Key Laboratory of High Technology for Plant Protection Plant Protection Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510640China department of Plant Pathology College of Plant ProtectionNanjing Agricultural Universityand Key Laboratory of Integrated Management of Crop Diseases and PestsMinistry of EducationNanjing 210095China State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Fujian Agriculture and Forestry UniversityFuzhou 350002China department of Biochemistry and Metabolism John Innes CentreNorwich Research ParkNorwich NR47UHUK

Arms race co-evolution of plant–pathogen interactions evolved sophisticated recognition mechanisms between host immune receptors and pathogen effectors. different allelic haplotypes of an immune receptor in the host mount distinct recognition against sequence or non-sequence related effectors in pathogens. We report the molecular characterization of the Piks allele of the rice immune receptor Pik against rice blast pathogen, which requires two head-to-head arrayed nucleotide-binding sites and leucine-rich repeat proteins. Like other Pik alleles, both Piks-1 and Piks-2 are necessary and sufficient for mediating resistance. However, unlike other Pik alleles, Piks does not recognize any known AvrPik variants of Magnaporthe oryzae. Sequence analysis of the genome of an avirulent isolate V86010 further revealed that its cognate avirulence(Avr) gene most likely has no significant sequence similarity to known AvrPik variants. Piks-1 and Pikm-1 have only two amino acid differences within the integrated heavy metal-associated(HMA) domain. Pikm-HMA interacts with AvrPik-A,-d, and-E in vitro and in vivo,whereas Piks-HMA does not bind any AvrPik variants. Characterization of two amino acid residues differing Piks-1 from Pikm-1 reveal that Piks-E229Q derived from the exchange of Glu229 to Gln229 in Piks-1 gains recognition specificity against AvrPik-d but not AvrPik-A or-E, indicating that Piks-E229Q partially restores the Pikm spectrum. By contrast,Piks-A261V derived from the exchange of Ala261 to Val261 in Piks-1 retains Piks recognition *** conclude that Glu229 in Piks-1 is critical for Piks breaking the canonical Pik/AvrPik recognition pattern. Intriguingly, binding activity and ectopic cell death induction is maintained between Piks-A261V and AvrPik-d, implying that positive outcomes from ectopic assays might be insufficient to deduce its immune activity against the relevant effectors in rice and rice blast interaction.

关键词： AvrPik co-evolution NBS-LRR Piks recognition

Enhanced π-π Interactions Between a C_(60) Fullerene and a Buckle Bend on a double-Walled Carbon Nanotube

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Nano Research 2010年第2期3卷 92-97页

作者： Sandeep Gorantla Stanislav Avdoshenko Felix Börrnert Alicja Bachmatiuk Maria dimitrakopoulou Franziska Schäffel Ronny Schönfelder jürgen Thomas Thomas Gemming jamie HWarner Gianaurelio Cuniberti jürgen Eckert Bernd Büchner mark H.Rümmeli IFW dresden P.O.Box 270116D-01171 DresdenGermany Institute for Materials Science and Max Bergmann Center of Biomaterials Dresden University of TechnologyDresden 01062Germany department of Materials University of OxfordParks Rd.Oxford OX13PHUnited Kingdom

In situ low-voltage aberration corrected transmission electron microscopy(TEM)observations of the dynamic entrapment of a C_(60) molecule in the saddle of a bent double-walled carbon nanotube is *** fullerene interaction is non-covalent,suggesting that enhancedπ-πinteractions(van der Waals forces)are *** molecular dynamics calculations confirm that the increased interaction area associated with a buckle is sufficient to trap a ***,they show hopping behavior in agreement with our experimental *** findings further our understanding of carbon nanostructure interactions,which are important in the rapidly developing field of low-voltage aberration corrected TEM and nano-carbon device fabrication.

关键词： Carbon nanotubes fullerenes low-voltage transmission electron microscopy molecule trap

Global blue carbon accumulation in tidal wetlands increases with climate change

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National Science Review 2021年第9期8卷 145-155页

作者： Faming Wang Christian j.Sanders Isaac R.Santos jianwu Tang mark Schuerch Matthew L.Kirwan Robert E.Kopp Kai Zhu Xiuzhen Li jiacan Yuan Wenzhi Liu Zhian Li Xiaoliang Research Station for Tropical Coastal Ecosystems Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems and the CAS Engineering Laboratory for Ecological Restoration of Island and Coastal Ecosystems South China Botanical Garden Chinese Academy of Sciences Center of Plant Ecology Core Botanical Gardens Chinese Academy of Sciences Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) State Key Laboratory of Estuarine and Coastal Research and Institute of Eco-Chongming East China Normal University National Marine Science Centre School of Environment Science and Engineering Southern Cross University department of Marine Sciences University of Gothenburg Lincoln Centre for Water and Planetary Health School of Geography University of Lincoln Virginia Institute of Marine Science College of William and Mary department of Earth and Planetary Sciences and Rutgers Institute of Earth Ocean and Atmospheric Sciences Rutgers University department of Environmental Studies University of California department of Atmospheric and Oceanic Sciences Fudan University CAS Key Laboratory of Aquatic Botany and Watershed Ecology Wuhan Botanical GardenChinese Academy of Sciences

Coastal tidal wetlands produce and accumulate significant amounts of organic carbon(C) that help to mitigate climate change. However, previous data limitations have prevented a robust evaluation of the global rates and mechanisms driving C accumulation. Here, we go beyond recent soil C stock estimates to reveal global tidal wetland C accumulation and predict changes under relative sea level rise, temperature and precipitation. We use data from literature study sites and our new observations spanning wide latitudinal gradients and 20 countries. Globally, tidal wetlands accumulate 53.65(95%CI: 48.52–59.01)Tg C yr-1, which is ～30% of the organic C buried on the ocean floor. Modeling based on current climatic drivers and under projected emissions scenarios revealed a net increase in the global C accumulation by2100. This rapid increase is driven by sea level rise in tidal marshes, and higher temperature and precipitation in mangroves. Countries with large areas of coastal wetlands, like Indonesia and Mexico, are more susceptible to tidal wetland C losses under climate change, while regions such as Australia, Brazil, the USA and China will experience a significant C accumulation increase under all projected scenarios.

关键词： coastal wetlands blue C C burial rate global change

An external quantum efficiency of>20%from solutionprocessed poly(dendrimer)organic light-emitting diodes

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npj Flexible Electronics 2018年第1期2卷 213-218页

作者： Fatemeh Maasoumi Ross d.jansen-van Vuuren Paul E.Shaw Emma V.Puttock Ravi Chandra Raju Nagiri jake A.McEwan mark Bown jenny L.O’Connell Christopher j.dunn Paul L.Burn Ebinazar B.Namdas Centre for Organic Photonics&Electronics(COPE) School of Chemistry&Molecular Biosciences and School of Mathematics&PhysicsUniversity of QueenslandSt.LuciaQLD 4072Australia CSIRO Manufacturing Research WayClaytonVIC 3168Australia

Controlling the orientation of the emissive dipole has led to a renaissance of organic light-emitting diode(OLEd)research,with external quantum efficiencies(EQEs)of>30%being reported for phosphorescent *** highly efficient OLEds are generally manufactured using evaporative methods and are comprised of small-molecule heteroleptic phosphorescent iridium(III)complexes blended with a host and additional layers to balance charge injection and *** area OLEds for lighting and display applications would benefit from low-cost solution processing,provided that high EQEs could be ***,we show that poly(dendrimer)s consisting of a non-conjugated polymer backbone with iridium(III)complexes forming the cores of firstgeneration dendrimer side chains can be co-deposited with a host by solution processing to give highly efficient *** bilayer devices comprising the emissive layer and an electron transport layer gave an EQE of>20%at luminances of up to≈300 cd/m^(2),showing that polymer engineering can enable alignment of the emissive dipole of solution-processed phosphorescent materials.

关键词： quantum dipole light

Male and female meadow voles Microtus pennsylvanicus respond differently to scent marks from the top- middle-, and bottom-scent donors of an over-mark

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Current Zoology 2011年第4期57卷 441-448页

作者： Michael H. FERKIN Nicholas j. HOBBS Benjamin d. FERKIN Adam C.FERKIN daniel A. FERKIN department of Biological Sciences The University of Memphis Ellington Hall Memphis TN 38152 USA

Previous studies have shown that individuals responded preferentially to the mark of the top-scent donor relative to that of the bottom-scent donor of an over-mark. However, terrestrial mammals are likely to encounter over-marks consisting of the scent marks of more than two same-sex conspecifics in the intersections of runways, near the nests of sexually receptive female conspecifics, and inside and along the borders of the territories of conspecifics. We determined how meadow voles, Microtus pennsylvanicus, respond to the marks of the top-, middle-, and bottom-scent donors of an over-mark. We tested the hypothesis that voles exposed to an over-mark will respond preferentially to the scent marks that were deposited more recently, the scent marks that were on top or near the top of the over-mark, compared to the scent marks that were deposited earlier or near the bot- tom of the over-mark. Voles spent more time investigating the mark of the top-scent donor than that of the either the middle- or bottom-scent donor. However, males but not female voles spent more time investigating the middle-scent mark than the bot- tom-scent mark. We also tested the hypothesis that voles evaluate and respond to over-marks differently from single scent marks. Voles spent more time investigating the marks of the top-, middle-, and bottom-scent donors compared to scent marks that were not part of the over-mark. Voles can distinguish among the overlapping scent marks of three scent donors and sex differences exist in the values they appear to attach to each of these scent marks [Current Zoology 57 （4）： 441-448, 2011 ].

关键词： Over-marks Voles Scent marks Olfactory communication

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解决磁化率填图问题的卡尔曼滤波器

世界地质 1989年第1期8卷 81-93页

作者： mark Pilkington d.j.Crossley 李松

当在分割成若干单元的场源区寻求磁化强度或磁化率分布时,在磁法解释中出现了线性反演问题。通常是用反褶积或矩阵方法求解。我们在本文提出一种简单的磁场产生过程的状态变量模型,在这个模型中中,地下被划分许多无限延深的垂直棱柱。... 详细信息

当在分割成若干单元的场源区寻求磁化强度或磁化率分布时,在磁法解释中出现了线性反演问题。通常是用反褶积或矩阵方法求解。我们在本文提出一种简单的磁场产生过程的状态变量模型,在这个模型中中,地下被划分许多无限延深的垂直棱柱。假定每个棱柱的磁化强度是邻近棱柱磁化强度的线性组合加上一个随机部分。用离散卡尔曼滤波器得到磁化强度分布的估计值。人工会成数据的试验表明该方法优于惯用的及褶积方法。该方法已被应用到Abitibi绿岩带的部分地区从而证明了在圈定本区主要岩性上的有效性。

关键词：卡尔曼滤波器磁化率滤波器参数磁法解释线性反演变量模型绿岩带线性组合主要岩性维纳滤波器