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Humoral Immune Response of Immunized Sows with Recombinant Proteins of Enterotoxigenic <i>Escherichia coli</i>

World Journal of Vaccines 2015年第1期5卷 60-68页

作者： Daniele Araujo Pereira Caio Abércioda Silva mario augusto ono Odilon Vidotto Marilda Carlos Vidotto Depto de Medicina Veterinária Preventiva Universidade Estadual de Londrina Londrina Brazil Depto de Zootecnia Universidade Estadual de Londrina Londrina Brazil Depto Ciências Patológicas Universidade Estadual de Londrina Londrina Brazil

Enteric disorders in pigs are related to the fimbriae F4 (K88), F5 (K99), F6 (987P), F41 and F18 of enterotoxigenic Escherichia coli (ETEC). Immunization of sows with adhesins is important to stimulate the production of antibodies and the consequent transfer of these to the piglets via colostrum to prevent diarrhea during the neonate period and after weaning. The objective of this study was to evaluate the immune response of the sows immunized with recombinant ETEC proteins (F4, F5, F6, F18 and F41). The immune response of the sows immunized with the recombinant proteins was compared with a commercial vaccine containing ETEC bacterins. The study was performed on a commercial farm and included nine pregnant sows divided into three groups: G1 was vaccinated with recombinant proteins (n = 3);G2 was vaccinated with the commercial vaccine (n = 3);and G3 was vaccinated with sterile buffered saline (PBS) (n = 3). All the sows were fed a balanced diet without antibiotics and water ad libitum. The recombinant fimbriae stimulated the specific humoral immune response of the immunized sows. There was a statistically significant increase in the levels of antibodies to the fimbriae F4 (K88), F5 (K99), F6 (987P) and F18 in the sows vaccinated with the recombinant proteins compared with the control group. The colostrum IgG titers for all fimbriae in all the immunized sows were significantly increased compared to the control group. Additionally, all the piglets exhibited significantly increased antibody levels relative to all fimbriae when compared with those in the unimmunized control group, demonstrating successful antibody transfer via colostrum of the sows to the piglets.

关键词： Humoral Immune Response ETEC Recombinant Fimbriae F4 F5 F6 F18 F41

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Weathering Trump2.0

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China Today 2025年第1期74卷 20-21页

作者： augusto SOTO Dialogue with China Project in Barcelona Spain

While the 47^(th) *** has declared a trade war on all nations,China has the strength to get ahead despite the complex rivalry.“History doesn’t repeat itself,but it often rhymes.”This saying by celebrated American writer Mark Twain,a contemporary of the American Civil War(1861-1865)and the Crimean War(1853-1856),resonates uncannily once again as Donald Trump takes charge in the United States.

关键词： Trump saying contemporary

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Potential of non-Western medicines in chemoradiotherapy for cervical cancer

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World Journal of Clinical Cases 2025年第2期13卷 21-23页

作者： Takashi ono Masashi Koto Department of Radiation Oncology Faculty of MedicineYamagata UniversityYamagata 990-9585Japan

This editorial explores the potential integration of non-Western medicine into radiotherapy for cervical *** radiotherapy remains a radical treatment for cervical cancer,its associated toxicity and decline in quality of life can significantly impact patients’***,most treatments are supportive,with no specific treatment options available in Western ***-Western medicine,often less toxic and easier to administer,has shown promising results when used alongside radiotherapy for cervical *** these potential benefits,challenges such as limited evidence and restricted application areas *** non-Western medicines may offer potential improvements in chemoradiotherapy outcomes for cervical cancer,further research is necessary to substantiate these benefits.

关键词： Cervical cancer Radiotherapy Non-Western medicine Kampo Herbal medicine

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Long-term experimental study on gravitational sedimentation of water aluminum oxide nanofluid at different volumetric concentrations

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International Journal of Sediment Research 2023年第3期38卷 303-315页

作者： mario Misale Johan augusto Bocanegra Annalisa Marchitto DIME-Department of Mechanics Energetics Management and Transportation Thermal Engineering and Environmental Conditioning Division University of Genoa

The stability of nanofluids is critical in engineering applications. The sedimentation of the nanoparticles in the base fluid limits the stability of the nanofluid. By measuring the absorbance of a visible laser through water aluminum oxide nanofluids it is possible to observe the evolution of the sedimentation process. A simple experimental setup consisting of a thin closed test cavity（0.8 mm or 1.5 mm thickness）filled with nanofluid and a set of laser diodes-photodiodes pairs was used in the experiments to determine the absorbance and the local volumetric concentration. The evolution of the local volumetric concentration of the nanofluid was measured at 13 height positions along the test cavity. Five initial volumetric concentrations of aluminum oxide（Al2O3; 0.5%, 1.0%, 1.5%, 2.0%, 3.0% and 4.0%） were considered. Data and digital photos were acquired for a total time lapse of 260 days. The results were independent of the container thickness. The same general trend was consistent for all the initial volumetric concentrations, i.e., the sedimentation rate decreases in time. The time evolution of the volumetric concentration was compared with results from a modified Mason-Weaver model. The comparison was possible by setting a variable sedimentation velocity in the model, suggesting that the sedimentation is affected by the dynamics of nanoparticle clusters with diverse sizes produced by agglomeration.

关键词： Pure nanofluid Aluminum oxide Nanoscale alumina particles Colloid sedimentation Nanoparticle settling Mason-Weaver model

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Reassessing the AMPK-MTORC1 balance in autophagy in the central nervous system

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Neural Regeneration Research 2025年第11期20卷 3209-3210页

作者： Marta García-Juan mario Villa Irene Benito-Cuesta Lara Ordónez-Gutiérrez Francisco Wandosell Centro de Biología Molecular“Severo Ochoa”(CSIC-UAM) Nicolas Cabrera 1Universidad Autónoma de MadridMadridSpain Fisiología Animal Departamento de BiologíaFacultad de CienciasUniversidad Autónoma de MadridMadridSpain Department of Clinical Neuroscience CMM Karolinska Universitetssjukhuset SolnaStockholmSweden Departamento de Bioquímica y Biología Molecular Universidad Complutense de MadridMadridSpain Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas(CIBERNED) MadridSpain

Autophagy is a cellular degradation and recycling system,indispensable for cellular and organ development,homeostasis,and *** cellular process is evolutionarily highly conserved to quality control of many proteins and dysfunctional organelles,which finally recycle components as amino *** process is effective during normal physiology as part of anabolism and plays an additional important role during starvation(Dikic and Elazar,2018).Different types of autophagy have been chara cterized based on their dynamic,mechanism of action,target substrates,and protein *** of them are macroautophagy(hereafter called"autophagy"),microa utophagy,and chape rone-media ted autophagy(Fleming et al.,2022).

关键词： homeostasis system hereafter

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High-Throughput Corn Image Segmentation and Trait Extraction Using Chlorophyll Fluorescence Images

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Plant Phenomics 2021年第1期3卷 129-143页

作者： augusto Souza Yang Yang Institute for Plant Sciences Purdue UniversityWest LafayetteINUSA

Plant segmentation and trait extraction for individual organs are two of the key challenges in high-throughput phenotyping(HTP)*** address this challenge,the Ag Alumni Seed Phenotyping Facility(AAPF)at Purdue University utilizes chlorophyll fluorescence images(CFIs)to enable consistent and efficient automatic segmentation of plants of different species,age,or color.A series of image analysis routines were also developed to facilitate the quantitative measurements of key corn plant traits.A proof-of-concept experiment was conducted to demonstrate the utility of the extracted traits in assessing drought stress reaction of corn *** image analysis routines successfully measured several corn morphological characteristics for different sizes such as plant height,area,top-node height and diameter,number of leaves,leaf area,and angle in relation to the *** from the proof-of-concept experiment showed how corn plants behaved when treated with different water regiments or grown in pot of different ***-throughput image segmentation and analysis basing on a plant’s fluorescence image was proved to be efficient and *** trait on the segmented stem and leaves of a corn plant demonstrated the importance and utility of this kind of trait data in evaluating the performance of corn plant under *** collected from corn plants grown in pots of different volumes showed the importance of using pot of standard size when conducting and reporting plant phenotyping data in a controlled-environment facility.

关键词： image sizes proof

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Data I/O management approach for the post-hoc visualization of big simulation data results

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International Journal of Modeling, Simulation, and Scientific Computing 2018年第3期9卷 77-92页

作者： Jorji Nonaka Eduardo C.Inacio Kenji ono mario A.R.Dantas Yasuhiro Kawashima Tomohiro Kawanabe Fumiyoshi Shoji RIKEN Advanced Institute for Computational Science Kobe Japan Kyushu University FukuokaJapan Federal University of Santa Catarina(UFSC)Florian´opolis Brazil Fujitsu NaganoJapan

Leading-edge supercomputers,such as the K computer,have generated a vast amount of simulation results,and most of these datasets were stored on the file system for the post-hoc analysis such as *** this work,we first investigated the data generation trends of the K computer by analyzing some operational log data *** verified a tendency of generating large amounts of distributed files as simulation outputs,and in most cases,the number of files has been proportional to the number of utilized computational nodes,that is,each computational node producing one or more *** that the computational cost of visualization tasks is usually much smaller than that required for large-scale numerical simulations,a flexible data input/output(I/O)management mechanism becomes highly useful for the post-hoc visualization and *** this work,we focused on the xDMlib data management library,and its flexible data I/O mechanism in order to enable flexible data loading of big computational climate simulation *** the proposed approach,a pre-processing is executed on the target distributed files for generating a light-weight metadata necessary for the elaboration of the data assignment mapping used in the subsequent data loading *** evaluated the proposed approach by using a 32-node visualization cluster,and the K *** the inevitable performance penalty associated with longer data loading time,when using smaller number of processes,there is a benefit for avoiding any data replication via copy,conversion,or *** addition,users will be able to freely select any number of nodes,without caring about the number of distributed files,for the post-hoc visualization and analysis purposes.

关键词： Scientific visualization distributed data data management K computer.

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Ultrafast reorientation of the Néel vector in antiferromagnetic Dirac semimetals

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npj Computational Materials 2021年第1期7卷 1562-1568页

作者： Atsushi ono Sumio Ishihara Department of Physics Tohoku UniversitySendai 980-8578Japan

Antiferromagnets exhibit distinctive characteristics such as ultrafast dynamics and robustness against perturbative fields,thereby attracting considerable interest in fundamental physics and technological ***,it was revealed that the Néel vector can be switched by a current-induced staggered(Néel)spin-orbit torque in antiferromagnets with the parity-time symmetry,and furthermore,a nonsymmorphic symmetry enables the control of Dirac ***,the real-time dynamics of the magnetic and electronic structures remain largely ***,we propose a theory of the ultrafast dynamics in antiferromagnetic Dirac semimetals and show that the Néel vector is rotated in the picosecond timescale by the terahertz-pulse-induced Néel spin-orbit torque and other torques originating from magnetic *** reorientation accompanies the modulation of the mass of Dirac fermions and can be observed in real time by the magneto-optical *** results provide a theoretical basis for emerging ultrafast antiferromagnetic spintronics combined with the topological aspects of materials.

关键词： materials Dirac metals

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Synergy of single-cell sequencing analyses and in vivo lineage-tracing approaches:A new opportunity for stem cell biology

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BIOCELL 2022年第5期46卷 1157-1162页

作者： YUKI MATSUSHITA WANIDA ono NORIAKI ono School of Dentistry University of Texas Health Science Center at HoustonHouston77054USA

Single-cell sequencing technologies have rapidly progressed in recent years,and been applied to characterize stem cells in a number of ***(postnatal)stem cells are generally identified using combinations of cell surface markers and transcription ***,it has been challenging to define micro-heterogeneity within“stem cell”populations,each of which stands at a different level of *** stem cells become defined at a single-cell level,their differentiation path becomes clearly ***,this viewpoint discusses the potential synergy of single-cell sequencing analyses with in vivo lineage-tracing approaches,with an emphasis on practical considerations in stem cell biology.

关键词： Single-cell sequencing in vivo lineage-lineage skeletal stem cells(SSCs) mesenchymal stem cells(MSCs) bone marrow stromal cells(BMSCs)

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Influence of oxygen flow rate and compost addition on reduction of organic matter in aerated waste layer containing mainly incineration residue

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Journal of Environmental Sciences 2013年第1期25卷 53-58页

作者： Hiroshi Asakura Kei Nakagawa Kazuto Endo Masato Yamada Yusaku ono Yoshiro ono Nagasaki University 1-14 Bunkyo-machi Nagasaki 852-8521Japan National Institute for Environmental Studies Onogawa 16-2TsukubaIbaraki 305-8506Japan Nippon Institute of Technology 4-1 GakuendaiMiyashiro-machiMinamisaitama-gunSaitama 345-8501Japan Kyoto Institute of Technology MatsugasakiSakyo-kuKyoto 606-8585Japan

Landfilling municipal solid waste incineration （MSWI） residue alkalizes the waste layer, causing a subsequent decrease in microbial activity and a delay in the decomposition of organic matter. In this study, efficiencies of neutralization of the leachate and organic matter decomposition in the waste layer in a column filled with MSWI residue using aeration and compost addition were evaluated. Total organic carbon （TOC） reduction in the waste layer is large at high oxygen flow rate （OFR）. To effectively accelerate TOC reduction in the waste layer to which compost was added, a high OFR exceeding that by natural ventilation was required. At day 65, the pH of the leachate when OFR was above 102 mol-O2/（day.m3） was lower than that when OFR was below 101 mol-Oz/（day.m3）. At the same OFR, the pH of waste sample was lower than that of waste sample with compost. Although leachate neutralization could be affected by compost addition, TOC reduction in the waste layer became rather small. It is possible that humic substances in compost prevent the decomposition of TOC in MSWI residue.

关键词： waste landfill aeration neutralization acceleration of stabilization

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