Synergy of single-cell sequencing analyses and in vivo lineage-tracing approaches:A new opportunity for stem cell biology

作     者：YUKI MATSUSHITA WANIDA ONO NORIAKI ONO 

作者机构：School of DentistryUniversity of Texas Health Science Center at HoustonHouston77054USA 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2022年第46卷第5期

页      面：1157-1162页

核心收录：

学科分类：0401[教育学-教育学] 04[教育学] 

基　　金：This research was supported by National Institute of Health Grants R01DE026666 and R01DE030630(to NO)and R01DE029181(to WO) 

主　　题：Single-cell sequencing in vivo lineage-lineage skeletal stem cells(SSCs) mesenchymal stem cells(MSCs) bone marrow stromal cells(BMSCs) 

摘      要：Single-cell sequencing technologies have rapidly progressed in recent years,and been applied to characterize stem cells in a number of ***(postnatal)stem cells are generally identified using combinations of cell surface markers and transcription ***,it has been challenging to define micro-heterogeneity within“stem cellpopulations,each of which stands at a different level of *** stem cells become defined at a single-cell level,their differentiation path becomes clearly ***,this viewpoint discusses the potential synergy of single-cell sequencing analyses with in vivo lineage-tracing approaches,with an emphasis on practical considerations in stem cell biology.