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Oxytocin in pig seminal plasma is positively related with in vivo fertility of inseminated sows

Journal of Animal Science and Biotechnology 2022年第2期13卷 430-440页

作者： lorena Padilla marina lópez-arjona Silvia Martinez-Subiela Heriberto Rodriguez-Martinez Jordi Roca Isabel Barranco Department of Medicine and Animal Surgery Faculty of Veterinary MedicineUniversity of MurciaE-30100 MurciaSpain Department of Biomedical&Clinical Sciences(BKV) BKH/Obstetrics&GynaecologyFaculty of Medicine and Health SciencesLinköping UniversitySE-58185 LinköpingSweden Department of Veterinary Medical Sciences University of BolognaIT-40064 Ozzano dell’EmiliaBolognaItaly

Background:Identification of relevant in vivo biomarkers for fertility remains a challenge for the livestock *** of the small peptide hormone oxytocin(OXT),involved in male reproductive function and present in the seminal plasma(SP)of several species could be a robust *** study characterized concentrations of SPOXT in ejaculates from boars used in artificial insemination(AI)programs aiming to evaluate its relationship with sperm quality variables and in vivo fertility of their liquid-stored *** OXT concentrations(ng/ml)were measured in 169 ejaculates from 61 boars of the Duroc,Pietrain,landrace and large White breeds using a direct competitive immunoassay test based on AlphalISA®***(ejaculate volume,sperm concentration,total sperm count)and sperm parameters(motility,viability,intracellular generation of reactive oxygen species,plasma membrane fluidity)were assessed at 0 h and 72 h in AI-semen samples stored at 17℃.In vivo fertility included only 18 large White and landrace boars whose AI-semen was used to inseminated>100 sows and evaluated both farrowing rate and litter size of 3,167 ***:The results showed that SP-OXT differed between boars and between ejaculates within boar(Plandrace and large White).Ejaculates with higher SP-OXT concentration/ml(hierarchically grouped;Plarger volume and came from younger boars(Plates of boars showing positive farrowing rate deviation exhibited higher(Pl than those with negative farrowing rate ***:The SP concentrations of OXT are boar,ejaculate and age dependent,and positively related with ejaculate volume and farrowing rates of liquid-stored semen AI-doses.

关键词： Artificial insemination Ejaculate Fertility Oxytocin Pig Semen quality Seminal plasma

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Adipose-derived mesenchymal stem/stromal cells:from the lab bench to the basic concepts for clinical translation

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BIOCEll 2018年第3期42卷 67-77页

作者： Yesica Romina FRONTINI-lópez Aldana Daniela GOJANOVICH Diego MASONE Diego Martín BUSTOS marina UHART laboratorio de Integración de Señles Celulares.IHEM-CONICET Universidad Nacional de Cuyo MendozaArgentina Facultad de Ingeniería Universidad Nacional de CuyoMendozaArgentina Facultad de Ciencias Exactas y Naturales Universidad Nacional de CuyoMendozaArgentina

In the last years,much work has shown that the most effective repair system of the body is represented by stem cells,which are defined as undifferentiated precursors that own unlimited or prolonged self-renewal ability,which also have the potential to transform themselves into various cell types through *** tissues that form the body contain many different types of somatic cells,along with stem cells that are called‘mesenchymal stem(or stromal)cells’(MSC).In certain circumstances,some of these MSC migrate to injured tissues to replace dead cells or to undergo differentiation to repair *** discovery of MSC has been an important step in regenerative medicine because of their high ***,the finding of a method to isolate MSC from adipose tissue,so called‘adipose-derived mesenchymal stem cells’(ASC),which share similar differentiation capabilities and isolation yield that is greater than other MSC,and less bioethical concerns compared to embryonic stem cells,have created self-praised publicity to procure almost any treatment with ***,we review the current techniques for isolation,culture and differentiation of human ASC(hASC),and describe them in *** also compile some advantages of the hASC over other stem cells,and provide some concepts that could help finding strategies to promote their therapeutic efficiency.

关键词： hASC Tissue regeneration Cell therapy

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Determination of l-norvaline and l-tryptophan in dietary supplements by nano-lC using an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11- dihydroquinidine-silica hybrid monolithic column

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Journal of Pharmaceutical Analysis 2020年第1期10卷 70-77页

作者： Dongsheng Xu Elena Sanchez-lopez Qiqin Wang Zhengjin Jiang María luisa marina Departamento de Química Analítica Química Física e Ingeniería QuímicaUniversidad de AlcalaCtra.Madrid-Barcelona Km.33.60028871Alcala de Henares(Madrid)Spain Institute of Pharmaceutical Analysis College of PharmacyJinan UniversityGuangzhou510632China Department of Pharmacy and Guangdong Province Key laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine&New Drug Research Jinan UniversityGuangzhou510632China Instituto de Investigacion Química“Andres M.del Río”(IQAR) Universidad de AlcalaCtra.Madrid-Barcelona Km.33.60028871Alcala de Henares(Madrid)Spain

An analytical methodology based on an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine(MQD)-silica hybrid monolithic column was developed for the enantioseparation of 9-fluorenylmethoxycarbonyl(FMOC)derivatized amino acids by nano-liquid *** mobile phase was optimized including the apparent pH,content of ACN,and concentration of the buffer to obtain a satisfactory enantioresolution performance.27 FMOC derivatized amino acids including 19 protein and 8 non-protein amino acids were tested,and 19 out of them were enantiomerically discriminated obtaining baseline separation for 11 of *** characteristics of the method were evaluated for norvaline and tryptophan in terms of linearity,precision,accuracy,limits of detection(lOD)and quantitation(lOQ)showing good performance to be applied to the enantiomeric determination of these amino acids in dietary *** and lOQ values were 9.3 and 31 mM for norvaline enantiomers and 7.5 and 25 mM for tryptophan enantiomers,*** contents of D-norvaline and D-tryptophan were below their respective lODs in all the analyzed *** of l-tryptophan and l-norvaline showed good agreement with the labeled contents except for one sample which did not show presence of l-norvaline,contrary to the label indication.

关键词： 9-Fluorenylmethoxycarbonyl Amino acids Chiral separation Nano-lC Quinidine-silica hybrid monolithic column

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Conservative management of perforated duodenal diverticulum: A case report and review of the literature

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World Journal of Gastroenterology 2008年第12期14卷 1949-1951页

作者： David Martínez-Cecilia Alvaro arjona Sánchez Manuel Gómez álvarez Eva Torres Tordera Antonio luque Molina Victor Valentí Azcárate Javier Briceo Delgado Francisco Javier Padillo Pedro lópez-Cillero Sebastián Rufián Pea Hospital Universitario Reina Sofia Av. Menendez Pidal sn. 14005. Córdoba Spain. Clínica Universitaria de Navarra Avenida de Pio XII 36 Pamplona 31008 Spain.

Duodenal diverticula are a relatively common condition. They are asymptomatic, unless they become complicated, with perforation being the rarest but most severe complication. Surgical treatment is the most frequently performed approach. We report the case of a patient with a perforated duodenal diverticulum, which was diagnosed early and treated conservatively with antibiotics and percutaneous drainage of secondary retroperitoneal abscesses. We suggest this method could be an acceptable option for the management of similar cases, provided that the patient is in good general condition and without septic signs.

关键词： Duodenal diverticula Perforation Conservativemanagement

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Comparison of bioavailability and transporters gene expression of four iron fortificants added to infant cereals

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Food Bioscience 2022年第6期50卷 291-299页

作者： Ana M.Caballero Valcárcel Rubén lópez Nicolás Carmen Frontela Saseta Carlos A.González Bermúdez Carmen Martínez Graciá marina Santaella Pascual Food Science and Technology Department Veterinary FacultyUniversity of MurciaRegional Campus of International Excellence Campus Mare NostrumUniversity of Murcia30100MurciaSpain

Iron deficiency is one of the most important nutritional disorders around the world. To reduce this health problem in infants, most countries have developed different iron fortification programs as a long-term strategy. However, it is necessary to select the best iron source to get the highest bioavailability with the lowest impact on the organoleptic properties of the foods. The aim of this research was to assess the in vitro bioavailability and intestinal cellular transport of four iron sources (electrolytic iron: EI, ferrous fumarate: FF, a micronized and dispersible ferric pyrophosphate: MDFP, and ferrous sulphate heptahydrated: FSH) used to fortify infant cereals. Fortified cereals were in vitro digested and soluble fractions were then incubated in Caco-2 cells to study iron retention, transport and uptake. Moreover, mRNA expression of iron transporters and ferritin formation were also measured in cells incubated with the iron standard salts. Iron solubility, uptake percentage, transport and uptake efficiencies by the Caco-2 cells were significantly higher in cereals fortified with FSH than with EI, obtaining intermediate values for the other two iron fortificants. MDFP induced higher expression of iron transporters Zip-14 and HEPH than other iron sources. FSH promoted the highest iron storage protein ferritin, followed by FF and MDFP, while EI presented the lowest in vitro bioavailability due to its low solubility. This work demonstrated that iron absorption, storage, and gene expression of transporters depend on the source of iron. These are important challenges to be taken into consideration when assessing the potential of iron fortificants.

关键词： Iron fortification Caco-2 cells Ferritin Gene expression Infant cereals Iron bioavailability

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Spontaneous liver rupture as first sign of polyarteritis nodosa

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World Journal of Hepatology 2016年第32期8卷 1414-1418页

作者： Irene Gómez-luque Felipe Alconchel Rubén Ciria M Dolores Ayllón Antonio luque marina Sánchez Pedro lópez-Cillero Javier Briceno General Surgery Reina Sofía University Hospital Department of Surgery Faculty of Medicine and NursingUniversity of Córdoba Unit of Hepatobiliary Surgery and liver Transplant Reina Sofía University Hospital Department of Pathology Reina Sofía University Hospital

Polyarteritis nodosa(PAN) is one of the systemic vasculitis that affects the media wall of arteries of small and medium diameter. Diagnosis proves difficult due to the unspecific symptoms that dominate the clinical profile. liver involvement is very diverse, ranging from the development of cirrhotic liver disease to acute abdomen presentation that requires surgery because of liver rupture. The management of these patients requires an expert multidisciplinary team. There are several cases in the literature that describe a sudden liver rupture as the first manifestation of a PAN. In this paper we present the case of a 75 years old patient without any previous disease, who is subjected to major hepatic resection for spontaneous liver rupture.

关键词： Polyarteritis nodosa Spontaneous liver rupture liver surgery Vasculitis Rheumatology

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Development of an Infusion Based on Romaine lettuce and Banana for Treating Insomnia

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Agricultural Sciences 2020年第6期11卷 529-539页

作者： Daniel Revelo-Cáceres María Jesús Oliveras-lópez marina Villalón-Mir Miguel Navarro-Alarcón Herminia lópez-García de la Serrana Facultad de Farmacia Universidad de Granada Granada Espa&#241 a Departamento de Biología Molecular e Ingeniería Bioquímica Universidad Pablo de Olavide Sevilla Espa&#241 a

An infusion was developed based on green romaine lettuce leaves and banana. Further, its sensorial acceptability was evaluated as a possible alternative treatment for insomnia. The romaine lettuces and bananas used were washed, chopped, dehydrated and ground. Optimal lettuce dehydration time was determined through charting the weight lost and examining the curve. This was found after 180 minutes at 60°C. Two formulas were developed, the first with 1.1 g of dehydrated romaine lettuce and 0.2 g of dehydrated banana, and the second with 1.1 g of dehydrated romaine lettuce and 0.4 g of dehydrated banana. Sensory analysis was conducted using sorting tests for taste, aroma, paired preferences and intention to purchase. Tasters preferred the formula with a lower quantity of dehydrated banana. 85% of tasters indicated that they would buy this infusion due to its potential functional properties for overcoming insomnia.

关键词： Infusion Romaine lettuce Banana Insomnia Sensory Analysis

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Cholecystokinin in the Pathogenesis of Bulimia Nervosa

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International Journal of Clinical Medicine 2011年第4期2卷 509-515页

作者： Helena Trebbau lópez Rosa María Molina-Ruiz laura Reyes Molón marina Díaz-Marsá 不详

Objective: This review aims to describe the role of the hormone cholecystokinin (CCK) in the pathogenesis of bulimia nervosa (BN), the perpetuation of this illness and the possibility of its use as a target for future therapeutic advances. Methods: Search for cholecystokinin AND bulimia nervosa in Pubmed Central, with no limits, identified 38 articles published up to the present date. Results: It is well established that CCK is altered in the pathogenesis of BN, and that its main role is in the perpetuation of the disorder rather than the cause of it. Discussion: Additional studies will be needed to further understand the mechanisms by which CCK regulates orexigenic pathways. If an orally active, longer acting analogue of CCK could be developed, it would be of significant interest as an appetite suppressant and a key adjuvant in the treatment of patients suffering from BN, particularly in refractory cases.

关键词： Satiety Cholecystokinin Bulimia Nervosa

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“Magic” of our gastric cancer results on perioperative chemotherapy

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World Journal of Gastrointestinal Pathophysiology 2016年第3期7卷 283-287页

作者： Carlos león-Espinoza Fernando lópez-Mozos Roberto Marti-Obiol marina Garces-Albir Joaquin Ortega-Serrano Department of Digestive and General Surgery Hospital Clínico Universitario de Valencia

AIM: To determine reproducibility of perioperative chemotherapy for gastric cancer(GC) on our settings by identifying patient's overall survival and comparing them to larger ***: Retrospective analysis of our series,where we present our eleven-year's experience on GC managed according to perioperative approach of three preoperative chemotherapy cycles followed by surgery and finally three postoperative chemotherapy *** scheme used was Xelox(Oxaliplatin and Capecitabine).Epidemiologic parameters as well as surgical variables were analysed,presented,and compared to other series with similar *** was estimated by Kaplan Meier/log rank method and also compared to these ***: Mean age was 65 years *** survival in our series was 37.7%,similar to other groups using perioperative *** was 4% and morbidity 30%,which are also similar to those *** curves were compared to larger studies,finding similarities on *** survival analysis between chemotherapy responders and non-responders didn't reach statically significant ***: Perioperative chemotherapic scheme can be reproduced on our setting with good results and without increasing morbidity or mortality.

关键词： Stomach neoplasms Perioperative period Chemotherapy

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Physicochemical and Functional Characterization of <i>Mucuna pruries</i>Depigmented Starch for Potential Industrial Applications

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International Journal of Organic Chemistry 2015年第1期5卷 1-10页

作者： Maira Rubi Segura-Campos Sonia marina lópez-Sánchez Arturo Castellanos-Ruelas David Betancur-Ancona luis Chel-Guerrero Facultad de Ingeniería Química Universidad Autónoma de Yucatán Mérida México

Starch is a very important biopolymer in the food industry. The velvet bean (M. pruriens) is an excellent potential starch source containing approximately 520 g starch per kg. The objective of this study was to evaluate the physicochemical and functional properties of velvet bean depigmented starch. The starch granules appear oval and spherical shaped. The colour registered l*, a*, b* values of 44.9, 0.324 and 0.341 respectively. The chemical composition registered values of moisture, ash, fat, protein, fibre and NFE of 110.5, 5.8, 5.7, 0.0, 34 and 954.5 g/kg respectively, as well as amylose levels of 215.3 g/kg. Gelatinization onset (To), peak (Tp) and final (Tf) temperatures were of 74.23°C, 80.57°C and 86.39°C. The solubility (3.1% - 16.2%), swelling power (SP) (2.86% - 16.17%) and water absorption capacity (WAC) (2.67 - 15.95 g water/g starch) were directly correlated to temperature (60°C - 90°C). The enthalpy values (4.10 - 13.47 j/g) were directly correlated to the time (1 - 21 days). The retrogradation increased as time increased. The viscosity of M. pruriens depigmented starch decreased slightly during the heating stages and then increased during cooling and the refrigeration and freezing stability registered syneresis ranges from 17.65 to 23.18 ml/50ml and from 16.4 to 22.6 ml/50ml respectively, indicating that the depigmented starch was unstable in heating-cooling processes.

关键词： Depigmented Starch Functional Properties Mucuna pruriens Physicochemical Properties

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