检索结果-南通市图书馆

Essential role of small bowel capsule endoscopy in reclassification of colonic inflammatory bowel disease type unclassified

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Endoscopy 2017年第1期9卷 34-40页

作者： Sara Monteiro Francisca Dias de Castro Pedro Boal Carvalho Bruno Rosa maria joao moreira Rolando Pinho Miguel Mascarenhas Saraiva José Cotter Department of Gastroenterology Hospital da Senhora da Oliveira-Guimaraes ManopH-Endo-scopy and Digestive Motility Laboratory Portugal and CUF Institute School of Medicine University of Minho ICVS/3B’s PT Government Associate Laboratory

AIM To evaluate the role of small bowel capsule endoscopy(SBCE) on the reclassification of colonic inflammatory bowel disease type unclassified(IBDU).METHODS We performed a multicenter, retrospective study including patients with IBDU undergoing SBCE, between 2002 and 2014. SBCE studies were reviewed and the inflammatory activity was evaluated by determining the Lewis score(LS). Inflammatory activity was considered significant and consistent with Crohn's disease(CD) when the LS ≥ 135. The definitive diagnosis during follow-up(minimum 12 mo following SBCE) was based on the combination of clinical, analytical, imaging, endoscopic and histological *** Thirty-six patients were included, 21 females(58%) with mean age at diagnosis of 33 ± 13(15-64) years. The mean follow-up time after the SBCE was 52 ± 41(12-156) mo. The SBCE revealed findings consistent with significant inflammatory activity in the small bowel(LS ≥ 135) in 9 patients(25%); in all of them the diagnosis of CD was confirmed during follow-up. In 27 patients(75%), the SBCE revealed no significant inflammatory activity(LS < 135); among these patients, the diagnosis of Ulcerative Colitis(UC) was established in 16 cases(59.3%), CD in 1 case(3.7%) and 10 patients(37%) maintained a diagnosis of IBDU during follow-up. A LS ≥ 135 at SBCE had a sensitivity = 90%, specificity = 100%, positive predictive value = 100% and negative predictive value = 94% for the diagnosis of *** SBCE proved to be fundamental in the reclassification of patients with IBDU. Absence of significant inflammatory activity in the small intestine allowed exclusion of CD in 94% of cases.

关键词： Inflammatory bowel disease Inflammatory bowel disease type unclassified Capsule endoscopy Crohn’s disease Lewis score Reclassification

Effects of natural mineral-rich water consumption on the expression of sirtuin 1 and angiogenic factors in the erectile tissue of rats with fructose-induced metabolic syndrome

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2014年第4期16卷 631-638页

作者： Cidalia D Pereira Milton Severo Luisa Rafael maria joao Martins Delminda Neves Department of Biochemistry Faculty of Medicine University of Porto Department of Clinical Epidemiology Predictive Medicine and Public Health Faculty of Medicine University of Porto Department of Clinical Pathology Sao Joao Hospital Centre EPE Department of Experimental Biology Faculty of Medicine and Institute for Molecular and Cell Biology (IBMC) University of Porto Portugal.

Consuming a high-fructose diet induces metabolic syndrome （MS）-Iike features, including endothelial dysfunction. Erectile dysfunction is an early manifestation of endothelial dysfunction and systemic vascular disease. Because mineral deficiency intensifies the deleterious effects of fructose consumption and mineral ingestion is protective against MS, we aimed to characterize the effects of 8weeks of natural mineral-rich water consumption on the structural organization and expression of vascular growth factors and receptors on the corpus cavernosum （CC） in 10% fructose-fed Sprague-Dawley rats （FRUCT）. Differences were not observed in the organization of the CC either on the expression of vascular endothelial growth factor （VEGF） or the components of the angiopoietins/Tie2 system. However, opposing expression patterns were observed for VEGF receptors （an increase and a decrease for VEGFR1 and VEGFR2, respectively） in FRUCT animals, with these patterns being strengthened by mineral-rich water ingestion. Mineral-rich water ingestion （FRUCTMIN） increased the proportion of smooth muscle cells compared with FRUCT rats and induced an upregulatory tendency of sirtuin I expression compared with the control and FRUCT groups. Western blot results were consistent with the dual immunofluorescence evaluation. Plasma oxidized low-density lipoprotein and plasma testosterone levels were similar among the experimental groups, although a tendency for an increase in the former was observed in the FRUCTMIN group. The mineral-rich water-treated rats presented changes similar to those observed in rats treated with MS-protective polyphenol-rich beverages or subjected to energy restriction, which led us to hypothesize that the effects of mineral-rich water consumption may be more vast than those directly observed in this study.

关键词： angiopoietins erectile tissue hypersaline sodium-rich naturally sparkling mineral water receptors sirtuin 1 vascularendothelial growth factor

New-onset diabetes after kidney transplantation:Incidence and associated factors

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Diabetes 2018年第7期9卷 132-137页

作者： Vania Gomes Florbela Ferreira JoséGuerra maria joao Bugalho Endocrinology Diabetes and Metabolism DepartmentSanta Maria HospitalLisbon 1649-035Portugal Nephrology and Kidney Transplantation Department Santa Maria HospitalLisbon 1649-035Portugal

AIM To determine the incidence and associated factors of new-onset diabetes after transplantation(NODAT) in a Portuguese central hospital. METHODS This single-center retrospective study involved consecutive adult nondiabetic transplant recipients, who had undergone kidney transplantation between January 2012 and March 2016. NODAT was diagnosed according to the criteria of the American Diabetes Association. Data were collected from an institutional database of the Nephrology and Kidney Transplantation Department(Santa maria Hospital, Lisbon, Portugal) and augmented with data of laboratorial parameters collected from the corresponding patient electronic medical records. Exclusion criteria were preexisting diabetes mellitus, missing information and follow-up period of less than 12 mo. Data on demographic and clinical characteristics as well as anthropometric and laboratorial parameters were also collected. Patients were divided into two groups: With and without NODAT-for statistical *** A total of 156 patients received kidney transplantduring the study period, 125 of who were included in our analysis. NODAT was identified in 27.2% of the patients(n = 34; 53% female; mean age: 49.5 ± 10.8 years; median follow-up: 36.4 ± 2.5 mo). The incidence in the first year was 24.8%. The median time to diagnosis was 3.68 ± 5.7 mo after transplantation, and 76.5% of the patients developed NODAT in the first 3 mo. In the group that did not develop NODAT(n = 91), 47% were female, with mean age of 46.4 ± 13.5 years and median follow-up of 35.5 ± 1.6 mo. In the NODAT group, the pretransplant fasting plasma glucose(FPG) levels were significantly higher [101(96.1-105.7) mg/d L vs 92(91.4-95.8) mg/d L, P = 0.007] and pretransplant impaired fasting glucose(IFG) was significantly more frequent(51.5% vs 27.7%, P = 0.01). Higher pretransplant FPG levels and pretransplant IFG were found to be predictive risk factors for NODAT development [odds ratio(OR): 1.059, P = 0.003; OR: 2.77

关键词： New-onset diabetes after transplant Incidence Kidney transplantation Impaired fasting glucose Immunosuppression

Effect of lead chloride on spermatogenesis and sperm parameters in mice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2004年第3期6卷 237-241页

作者： António Graca joao Ramalho-Santos maria de Lourdes Pereira Department of Zoology University of CoimbraCoimbraPortugal Department of BiologyUniversity of Aveiro3810 AveiroPortugalDepartment of ZoologyUniversity of CoimbraCoimbraPortugalDepartment of BiologyUniversity of Aveiro3810 AveiroPortugal

Aim: To evaluate the effect of acute lead chloride exposure on testis and sperm parameters in mice. Methods: PbCl2, 74 mg/kg, was daily administered to sexually mature male mice for 3 days and the effects on the testicular histology and ultrastructure as well as the motility and density of spermatozoa in cauda epididymis were observed. An additional group of mice were treated for 1-3 days and were allowed to recover for 32 days to determine the reversibility of lead-induced changes. Results: The testicular weight, seminiferous tubular diameter and sperm counts were significantly decreased following 3 days of PbCl2 treatment, but were unaffected by shorter-term exposures. The changes caused by lead are mostly reversible. Conclusion: Acute lead chloride exposure injures the fertility parameters of male mice and the effects are partially reversible.

关键词： lead chloride seminiferous tubules epididymis semen analysis

Rat cardiomyocyte H9c2(2-1)-based sulforhodamine B assay as a promising in vitro method to assess the biological component of effluent toxicity

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2020年第10期32卷 163-170页

作者： Elsa T Rodrigues Susana F Nascimento maria joao Moreno Paulo J Oliveira Miguel A Pardal University of Coimbra Centre for Functional EcologyDepartment of Life SciencesCalcada Martim de FreitasCoimbra 3000-456Portugal University of Coimbra Coimbra Chemistry CenterDepartment of ChemistryCoimbra 3004-535Portugal Centre for Neuroscience and Cell Biology Uniuersity of CoimbraUC BiotechBiocant ParkCantanhede 3060-197Portugal

The treatment of wastewaters is crucial to maintain the ecological status of receiving waters,and thereby guarantee the protection of aquatic life and human *** quality evaluation is conventionally based on physicochemical parameters,but increasing attention has been paid to integrate physicochemical and biological ***,the regulatory use of fish in biological testing methods has been subject to various ethical and cost concerns,and in vitro cell-based assays have thus become an important topic of ***,the present study intends:(a) to evaluate the efficiency of two different sample pre-concentration techniques (lyophilisation and solid phase extraction) to assess the toxicity of municipal effluents on rat cardiomyoblast H9c2(2-1) cells,and (b) maximizing the use of the effluent sample collected,to estimate the environmental condition of the receiving *** gathered results demonstrate that the H9c2(2-1) sulforhodamine B-based assay is an appropriate in vitro method to assess biological effluent toxicity,and the best results were attained by lyophilising the sample as *** to its response,the H9c2(2-1) cell line might be a possible alternative in vitro model for fish lethal testing to assess the toxicity of municipal *** physicochemical status of the sample suggests a high potential for eutrophication,and iron exceeded the permissible level for wastewater discharge,possibly due to the addition of ferric chloride for wastewater *** general,the levels of carbamazepine and sulfamethoxazole are higher than those reported for other countries,and both surpassed the aquatic protective values for long-term exposure.

关键词： Municipal wastewater treatment plant effluent Lyophilisation Solid phase extraction Cell-based assays H9c2(2-1)cell line SRB assay

Animal models of acute gastric mucosal injury: Macroscopic and microscopic evaluation

在线全文

学校读者我要写书评

暂无评论

Animal Models and Experimental Medicine 2019年第2期2卷 121-126页

作者： Sandra Simoes Rui Lopes maria Celeste Dias Campos maria joao Marruz maria Eugenia Meirinhos da Cruz Luisa Corvo Faculdade de Farmacia Instituto de Investigacao do Medicamento (iMed. ULisboa) Universidade de Lisboa Lisboa Portugal Servico de Anatomia Patologica Hospital Curry Cabral Lisboa Portugal ANATOMIK Avenida Campo Grande 168 1700‐094 Lisboa Portugal Hospital do Mar Bobadela Portugal

Acute gastric mucosal injury is a common disorder of the gastrointestinal tract and the search for new therapeutics is ongoing. The aim of this study is to update and expand the information related to the most widely used rat models of acute gastric ulcer, the ethanol‐induced ulcer and the indomethacin‐induced ulcer. These two models are compared in terms of macroscopic and microscopic features. Experimentally, ethanol was given orally in a single dose and indomethacin was subcutaneously injected into male Wistar rats. After ulcerative challenges, the stomachs were removed and visually inspected. Anti‐ulcerative drugs were used to validate the models. Histological analysis of the stomachs determined the microscopic score. The methodology used for model evaluation applied to macroscopic and microscopic gastric lesions. With these methods it was possible to induce lesions in the gastric mucosa. Microscopic evaluation permitted assessment of the inflammatory and apoptotic impact in the mucosa not observable by macroscopic evaluation. Groups of animals were treated with two standard drugs: sulcralfate suspension or lansoprazole solution. Both drugs reduced macroscopic and microscopic lesions, particularly the hemorrhagic ones. Both models induced acute gastric mucosal injury and no single evaluation method can address all the aspects of the pathology of gastric lesions. As a complement to macroscopic evaluation, microscopy appears to be a relevant tool to selectively identify specific aspects of the development of mucosal injury, quantify the extent of lesions, and contribute to an appropriate interpretation of results. The score systems established here offer a reliable method for testing antiulcer drugs.

关键词： animal models gastric ulcer histology pre-clinical assays

Common bean SNP alleles and candidate genes affecting photosynthesis under contrasting water regimes

在线全文

学校读者我要写书评

暂无评论

Horticulture Research 2021年第1期8卷 110-123页

作者： Susana Trindade Leitao maria Catarina Bicho Priscila Pereira maria joao Paulo Marcos Malosetti Susana de Sousa Araujo Fred van Eeuwijk maria Carlota Vaz Patto Instituto de Tecnologia Quimica e Biológica Antonio Xavier Universidade Nova de LisboaOeirasPortugal Wageningen University&Research WageningenThe Netherlands Present address:Instituto Superior de Agronomia Universidade de LisboaLisboaPortugal Present address:Nunhems Vegetable Seeds NunhemThe Netherlands Present address:Association BLC3-Technology and Innovation Campus Centre Bio R&D UnitOliveira do HospitalLisboaPortugal

Water deficit is a major worldwide constraint to common bean(Phaseolus vulgaris L.)production,being photosynthesis one of the most affected physiological *** gain insights into the genetic basis of the photosynthetic response of common bean under water-limited conditions,a collection of 158 Portuguese accessions was grown under both well-watered and water-deficit *** gas-exchange parameters were measured and photosynthetic pigments *** same collection was genotyped using SNP arrays,and SNP-trait associations tested considering a linear mixed model accounting for the genetic relatedness among accessions.A total of 133 SNP-trait associations were identified for net CO 2 assimilation rate,transpiration rate,stomatal conductance,and chlorophylls a and b,carotenes,and xanthophyll *** of these associations were detected under water-deficit and 43 under well-watered conditions,with only two associations common to both *** candidate genes revealed that stomatal regulation,protein translocation across membranes,redox mechanisms,hormone,and osmotic stress signaling were the most relevant processes involved in common bean response to water-limited *** candidates are now preferential targets for common bean water-deficit-tolerance ***,new sources of water-deficit tolerance of Andean,Mesoamerican,and admixed origin were detected as accessions valuable for breeding,and not yet explored.

关键词： breeding water assimilation

High-precision geochronology of Mesozoic magmatism in Macao,Southeast China:Evidence for multistage granite emplacement

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Geoscience Frontiers 2020年第1期11卷 243-263页

作者： Pedro Quelhas Agata Alveirinho Dias joao Mata Donald Wayne Davis maria Luísa Ribeiro Institute of Science and Environment University of Saint JosephMacaoChina Instituto Dom Luiz Faculdade de CiênciasUniversidade de LisboaLisboaPortugal Jack Satterly Geochronology Laboratory Department of Earth SciencesUniversity of TorontoTorontoOntCanada Laboratório Nacional de Engenharia e Geologia LisboaPortugal

Six new high precision U-Pb zircon ID-TIMS ages plus thirteen in situ high spatial resolution U-Pb zircon LA-MC-ICPMS ages are reported from Jurassic plutonic(metaluminous to weakly peraluminous biotite granites)and Jurassic to Cretaceous hypabyssal(dacites)rocks from *** its relatively small area(~30 km^2),the new ages tightly constrain the Macao granitic magmatism to two periods ranging from 164.5±0.6 Ma to 162.9±0.7 Ma and 156.6±0.2 Ma to 155.5±0.8 Ma,separated by ca.6 *** zircons point to the existence of a basement with ages up to Paleo-Proterozoic and late Archean in the *** addition,younger dacitic rocks were dated at 150.6±0.6 Ma and<120 Ma.U-Pb zircon ages and whole-rock REE data of Macao granites indicate that the first pulse is also represented in Hong Kong and Southeast(SE)China,while magmatism with the chemical characteristics of the second pulse seems to not be represented outside *** two granitic magmatic pulses have distinct mineralogical and geochemical features that support their discrete nature rather than a continuum of comagmatic activity and suggest that the Macao granitic suite was incrementally assembled during a period of ca.9 Ma,a hypothesis also extendable to the neighboring Hong Kong region for a time lapse of ca.24 *** Macao,the transition from granitic magmatism(Middle to Upper Jurassic)to the younger dacite dykes(Upper Jurassic to Lower Cretaceous)most likely corresponds to a change in the regional tectonic setting,from an extensional regime related with foundering of the subducting paleoPacific plate during the Early Yanshanian period to the reestablishment of a normal subduction system in SE China during the Late Yanshanian period.

关键词： Granites U-Pb geochronology Zircon Jurassic Yanshanian orogeny Macao

Unlocking the in vitro anti-Trypanosoma cruzi activity of halophyte plants from the southern Portugal

在线全文

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2016年第8期9卷 714-719页

作者： Marta Oliveira Policarpo Ademar Sales Junior maria joao Rodrigues Marina DellaGreca Luisa Barreira Silvane maria Fonseca Murta Alvaro Jose Romanha Luisa Custodio Center of Marine Sciences (CCMAR) University of AlgarveCampus de Gambelas 8005-139 FaroPortugal Centro de Pesquisas Rene Rachou FIOCRUZ Belo HorizonteMinas GeraisBrazil Dipartimento di Chimica Organica e Biochimica Universita` Federico Ⅱ Complesso Universitario Monte Sant'AngeloVia Cintia 4I-80126NapoliItaly

Objective:To evaluate the in vitro anti-Trypanosoma cruzi（***） activity of organic extracts prepared from halophyte species collected in the southern coast of Portugal（Algarve）,and chemically characterize the most active ***:Acetone,dichloromethane and methanol extracts were prepared from 31 halophyte species and tested in vitro against trypomastigotes and intracellular amastigotes of the Tulahuen strain of *** most active extract was fractionated by preparative HPLC-DAD,affording 11 *** most selective fraction was fully characterized by ***:From 94 samples tested,one was active,namely the root dichloromethane extract of Juncus acutus(IC50 < 20 μg/mL).This extract was fractionated by HPLC,affording 11 fractions,one of them containing only a pure compound（juncunol）,and tested for anti-parasitic *** 8(IC50 = 4.1 μg/mL) was the most active,and was further characterized by *** major compounds were phenanthrenes,9,10-dihydrophenanthrenes and ***:Our results suggest that the compounds identified in fraction 8 are likely responsible for the observed anti parasitic *** research is in progress aiming to isolate and identify the specific active *** the best of our knowledge,this is the first report on the in vitro anti *** activity of halophyte species.

关键词： Chagas disease Halophytes Trypanosoma cruzi Phenanthrenes Juncunol