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Antioxidant Action of Rosemary and Oregano Extract in Pre-Cooked Meat Hamburger

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Food and Nutrition Sciences 2018年第7期9卷 806-817页

作者： maria Raquel Manhani maria aparecida nicoletti Andrea Carla Da Silva Barretto Graciele Riveres De Jesus Camila Munhoz Gabriel Ramos De Abreu Julia Zaccarelli-Magalhaes Andre Rinaldi Fukushima Instituto Federal de Sã o Paulo(IFSP) Campus SuzanoSuzanoBrazil Faculty of Pharmaceutical Sciences University of Sao PauloSao PauloBrazil Instituto de Biociencias Letras e Ciências Exatas de Sao Jose do Rio Preto Universidade Estadual Paulista Júlio de Mesquita FilhoSao Josédo Rio PretoBrazil Universidade Sao Judas Tadeu Sao PauloBrasil Department of Pathology School of Veterinary Medicine and Animal ScienceUniversity of Sao PauloSao PauloBrazil Health Science Institute Presbiterian Mackenzie UniversitySao PauloBrazil

The main goal is to compare the antioxidant potential of rosemary and oregano natural extracts in precooked beef burger by assessing the lipid oxidation extent and sensory analysis. Five formulations (F) of hamburger were prepared from beef and mechanically separated as follows: meat containing sodium erythorbate (F1);deodorized rosemary extract (F2);oregano extract (F3);rosemary plus oregano extracts (F4) and without antioxidant addition, denominated control formulation (CF). The samples were frozen at ?18°C for 24 hours, then submitted to heat treatment in an electric oven with internal controlled temperature of 75°C, and again frozen for a period of 30 days. The lipid oxidation extent (determined by thiobarbituric acid reactive substances— TBRS) was evaluated at 0, 15 during 30 days. After heating in plate to temperature of 75°C, the samples were submitted to color, taste and odor evaluation by 40 untrained tasters. The formulations F1, F2 and F4 presented lower concentrations of TBRS, whereas CF at day zero already showed very high values, indicating oxidation of the product. The samples showed good acceptance in the sensorial analysis.

关键词： Rosemary Extract Oregano Extract Food Composition Food Preservation Food Quality Meat Product

Cassava Flour: Quantification of Cyanide Content

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Food and Nutrition Sciences 2016年第7期7卷 592-599页

作者： André Rinaldi Fukushima maria aparecida nicoletti Almir Junior Rodrigues Caroline Pressutti Jeandro Almeida Tamires Brandão Rosilene Kinue Ito Luis Antônio Bafille Leoni Helenice De Souza Spinosa USJT-Pharmacy Course Universidade S&#227o Judas Tadeu Campus Capital S&#227o Paulo Brazil FMVZ-School of Veterinary Medicine and Animal Science Universidade de S&#227o Paulo Campus Capital S&#227o Paulo Brazil Pharmacy Course Universidade Guarulhos-Ser Educacional Campus Centro S&#227o Paulo Brazi Departamento de Farmácia Faculdade de Ciências Farmacêuticas Universidade de S&#227o Paulo Campus Capital S&#227o Paulo Brazil

Cassava flours are obtained from the roots of Manihot esculenta Crantz and daily fairly consumed by the Brazilian population considering it comes to hum food, and also inserted into food baskets, besides to present low cost and easy access, in addition to being plant easily cultivated by the population. However, the food obtained with cassava may contain hydrocyanic acid, in the form of cyanogenic glycoside when those meals are not cooked properly. The oral toxicity can range 30 - 210 mg/kg body, the average daily consumption per capita in larger rural areas than in urban areas for cassava flour 19.1 g against 4.7 g, respectively. Brazil’s North and Northeast have higher consumption of cassava flour, associated with family monthly monetary income, making this region more vulnerable to poisoning. The objective of this study was to quantify cyanogenic glycosides present in some types of Brazilian cassava foods by means of spectrophotometric technique (reading at a wavelength of 530 nm). For quantitative determination of cyanide content, linamarase hydrolysis was used. The amount of cyanide was determined, and results were: artisan toasted cassava flour: 15 mg/500g, sweet cassava starch: 32.5 mg/500g, artisan dried cassava flour: 37.5 mg/500g, “bijuzada” cassava flour: 60 mg/500g, industrialized, toasted cassava flour: 115 mg/500g, industrialized, raw cassava flour: 140 mg/500g, and wet cassava flour: 225 mg/500g. Considering the chronic ingestion of cyanide present in these foods, they can cause public health problems.

关键词： Manihot esculenta Crantz Manihot Flour Cyanides Toxicity Spectrophotometry Konigi Method

Determination of Paraquat in Several Commercially Available Types of Rice

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Food and Nutrition Sciences 2018年第12期9卷 1368-1375页

作者： Thais Lopes Lima maria aparecida nicoletti Camila Munhoz Gabriel Ramos De Abreu Julia Zaccarelli Magalhaes Esther Lopes Ricci Ricci Paula A.Faria Waziry Júlia Nathalia Alves da Costa Ana Carolina Nascimento Antonio AndréRinaldi Fukushima Universidade Sao Judas Tadeu-R.Taquari Sao Paulo/SPBrazil Departamento de Farmacia.Faculdade de Ciencias Farmaceuticas.Universidade de Sao Paulo Sao Paulo/SPBrazil Instituto de Ciencias da Saude Universidade Presbiteriana MackenzieRua da ConsolacaoSao Paulo/SPBrazil Departamento de Patologia Faculdade de Medicina Veterinaria e ZootecniaUniversidade de Sao PauloSao Paulo/SPBrazil New Southeastern University Fort LauderdaleFloridaUnited States of America Veterans Affairs Medical CenterMiamiFloridaUSA Faculdade Sao Bento-Largo de Sao Bento s/no-CentroSao Paulo-SPSao Paulo/SPBrazil

Introduction: Paraquat (1,1’-dimethyl-4,4’-bipyridine-dichloride) is a herbicide used to combat weeds and improve crop productivity. Its acute toxicity can cause fatal poisoning in humans and animals and several studies have shown a strong correlation between paraquat and Parkinson’s disease. Objective: To determine the concentration of paraquat in different types of rice in Brazil. Method: Paraquat quantification is based on a complexation reaction with sodium dithionite, which generates a blueish color compound, whose absorbance was read in a spectrophotometer at the 600 nm wavelength. Results: Five samples were found to be above the maximum allowed limit, while one sample had a concentration in the upper allowed limit. The method employed did not allow quantification of black rice. Conclusion: There is a need for identification of the type of pesticide used in each examined crop as well as their respective dates of remission in order to improve the safety of agricultural practices.

关键词： Paraquat Herbicide Rice

Analysis of the genotoxic potential of low concentrations of Malathion on the Allium cepa cells and rat hepatoma tissue culture

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Journal of Environmental Sciences 2015年第10期27卷 102-111页

作者： Jaqueline Bianchi Mario Sérgio Mantovani maria aparecida Marin-Morales Department of Biology Institute of BiosciencesSao Paulo State University(UNESP) Department of General Biology Biological Science CentreUniv Estadual de Londrina

Based on the concentration of Malathion used in the field, we evaluated the genotoxic potential of low concentrations of this insecticide on meristematic and F1 cells of Allium cepa and on rat hepatoma tissue culture（HTC cells）. In the A. cepa, chromosomal aberrations（CAs）, micronuclei（MN）, and mitotic index（MI） were evaluated by exposing the cells at 1.5,0.75, 0.37, and 0.18 mg/m L of Malathion for 24 and 48 hr of exposure and 48 hr of recovery time. The results showed that all concentrations were genotoxic to A. cepa cells. However,the analysis of the MI has showed non-relevant effects. Chromosomal bridges were the CA more frequently induced, indicating the clastogenic action of Malathion. After the recovery period, the higher concentrations continued to induce genotoxic effects, unlike the observed for the lowest concentrations tested. In HTC cells, the genotoxicity of Malathion was evaluated by the MN test and the comet assay by exposing the cells at 0.09, 0.009, and0.0009 mg/5 m L culture medium, for 24 hr of exposure. In the comet assay, all the concentrations induced genotoxicity in the HTC cells. In the MN test, no significant induction of MN was observed. The genotoxicity induced by the low concentrations of Malathion presented in this work highlights the importance of studying the effects of low concentrations of this pesticide and demonstrates the efficiency of these two test systems for the detection of genetic damage promoted by Malathion.

关键词： HTC Chromosomal aberration Micronuclei Comet assay Pesticide

Microbial lipases:Propitious biocatalysts for the food industry

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Food Bioscience 2022年第1期45卷 34-44页

作者： Cleonice aparecida Salgado Clarissa Isabela aparecida dos Santos maria Cristina Dantas Vanetti Department of Microbiology Universidade Federal de Viçosa(UFV)ViçosaMGBrazil

Lipases(triacylglycerol acylhydrolases,EC 3.***.1.3)are one of the largest groups of enzymes and are used in various industrial *** of microbial origin are currently receiving increased attention for industrial application as microorganisms grow quickly and are easily genetically ***,they offer several advantages,such as catalysis of diverse reactions,high specificity,high yields,low energy consumption and reduced processing time and production *** is a relentless ongoing effort to optimise the production of microbial lipases for potential application in the food *** this context,this review highlights the most promising techniques for producing microbial lipases and the recent applications of these lipases in dairy,oils and fats,bakery and confectionery,meat,flavours and aromas and other food *** lipases are normally obtained by fermentation,but the high costs of carbon and nitrogen sources limit the *** overcome this problem,low-cost agro-industrial residues in the lipase production process are *** obtain lipases with high yields and improved characteristics,the technique of protein engineering is described as promising,and the immobilization method that allows the recycling of lipases to improve their catalytic performance is *** to their catalytic properties and versatility,lipases of microbial origin are considered extremely important catalysts in the food industry,meeting the demand for tastier foods with pleasant aromas and ***,microbial lipases are considered safe and sustainable biocatalysts.

关键词： Microbial lipases Food industry Agro-industrial residue

Rare earth metal as a dopant element:Cerium ion as an articulator in hexavalent chromium removal by magnetic iron oxides

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Journal of Rare Earths 2023年第10期41卷 1616-1626,I0006页

作者： Pricila maria Batista Chagas Maísa M.M.Lima Aline aparecida Caetano Lucas Bragança Carvalho Nayra maria de Abreu Piva maria Eduarda Resende Luiz Iara do Rosário Guimarães Laboratory of Environmental Catalysis and New Materials Department of ChemistryFederal University of LavrasCEP 37200-900LavrasMGBrazil Department of Viticulture&Enology University of CaliforniaDavisCA95616USA Institute of Science and Technology São Paulo State University18087-180SorocabaBrazil

This study highlights introducing the rare earth metal cerium(Ce(Ⅳ))into the structure of magnetite(Fe_(3)O_(4))to achieve enhanced adsorptive properties for the removal of chromium(Cr(VI))from an aqueous *** ratios of Ce(Ⅳ)were introduced into the iron oxide matrix,termed FeCe-5,FeCe-10,and *** numerical values correspond to the nominal content of the dopant element added to the synthesis *** solid materials were characterized for morphology and chemical structure,and N_(2)physical adsorption/desorption measurements were *** solid materials doped with Ce(IV)have a high surface area compared to Fe_(3)O_(4),and the solid material with the highest content of the dopant ion(Ce(IV))has a 4-fold greater surface *** increase in the dopant content in the iron oxide structure leads to a total chromium removal of 93.3%.Isotherms studies on the solid materials show that chromium adsorption follows the Langmuir *** adsorption capacity to Fe_(3)O_(4)is 12.59 mg/g and FeCe-10 is 22.49 mg/g at 35℃.By fitting the kinetic and isothermal models,it is found that for the Fe_(3)O_(4)and FeCe-10 materials Cr(VI)removal occurs in very different ways,attributed to the different surface areas and compositions of the oxide,with the formation of the goethite(α-FeOOH)*** FeCe-10reuse process was performed and the removal capacity the Cr(VI)is reduced after the first *** result is attributed to a strong and irreversible adsorption of Cr(VI)on the FeCe-10.

关键词： Bimetallic oxides Fe-Ce interaction Oxygen vacancies Adsorption Cr(Ⅵ) Rare earths

Perivascular epithelioid cell tumor of the liver coexisting with a gastrointestinal stromal tumor

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World Journal of Gastroenterology 2008年第5期14卷 800-802页

作者： Carlos Eduardo Paiva Francisco Alves Moraes Neto Abbas Agaimy maria aparecida Custódio Domingues Silvia Regina Rogatto Oncological and Hemato-oncological Center So Paulo State University Botucatu So Paulo 18610-170 Brazil Department of Pathology Amaral Carvalho Hospital Jaú So Paulo Brazil Institute of Pathology Nuremberg Clinic Center Nuremberg Germany Department of Pathology So Paulo State University Botucatu So Paulo 18610-170 Brazil Department of Urology So Paulo State University Botucatu So Paulo 18610-170 Brazil

Approximately 10% of patients with gastrointestinal stromal tumors (GIST) develop other neoplasms, either synchronously or metachronously. In this report we describe coexistence of a gastrointestinal stromal tumor and a hepatic perivascular epithelioid cell tumor (PEComa) in a 51-year-old woman with no evidence of tuberous sclerosis. A subcapsular hepatic nodule (0.8 cm in diameter) was found during surgery for symptomatic gastric neoplasm (15 cm in diameter) arising from the lesser curvature. Both tumors revealed histomorphological and immunohistochemical features confirming a diagnosis of a small incidental hepatic PEComa and a high risky extramural gastric GIST, respectively. The patient remained disease-free 25 mo after surgery with no evidence of tumor recurrence or new neoplasms. To our knowledge, this is the first report of PEComa in a patient with GIST. Hepatic lesions detected synchronously or metachronously in patients with GISTs may represent histogenetically distinct lesions and should be sampled to confirm or exclude metastatic GISTs.

关键词： Gastrointestinal stromal tumors PEComa Coexistence Liver Synchronous

Elastography as a predictor of liver cirrhosis complications after hepatitis C virus eradication in the era of direct-acting antivirals

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World Journal of Hepatology 2021年第11期13卷 1663-1676页

作者： Lucia Cerrito maria Elena Ainora Alberto nicoletti Matteo Garcovich Laura Riccardi Maurizio Pompili Antonio Gasbarrini maria Assunta Zocco CEMAD Digestive Disease Center Fondazione Policlinico Universitario"A.Gemelli"IRCCSCatholic University of Rome(Italy)Rome 00168Italy

Chronic inflammation due to hepatitis C virus(HCV)infection leads to liver fibrosis and rearrangement of liver tissue,which is responsible for the development of portal hypertension(PH)and hepatocellular carcinoma(HCC).The advent of direct-acting antiviral drugs has revolutionized the natural history of HCV infection,providing an overall eradication rate of over 90%.Despite a significant decrease after sustained virological response(SVR),the rate of HCC and liver-related complications is not completely eliminated in patients with advanced liver *** the reasons are still unclear,cirrhosis itself has a residual risk for the development of HCC and other PH-related *** elastography is a recently developed non-invasive technique for the assessment of liver *** the achievement of SVR,liver stiffness(LS)usually decreases,as a consequence of reduced inflammation and,possibly,*** studies emphasized the application of LS assessment in the management of patients with SVR in order to define the risk for developing the complications of chronic liver disease(functional decompensation,gastrointestinal bleeding,HCC)and to optimize long-term prognostic outcomes in clinical practice.

关键词： Direct-acting antiviral agents Liver stiffness Portal hypertension Hepatocellular carcinoma

Litterfall dynamics and soil carbon and nitrogen stocks in the Brazilian palm swamp ecosystems

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Forest Ecosystems 2020年第3期7卷 499-510页

作者： Gracielle de Brito Sales Taynan Aquilles Marinho Lessa Daniela aparecida Freitas maria das Dores Magalhães Veloso maria Ligia de Souza Silva Luiz Arnaldo Fernandes Leidivan Almeida Frazão Instituto de Ciências Agrárias Universidade Federal de Minas GeraisAvenida Universitária1000Montes ClarosMG39404-547Brazil Universidade Estadual de Montes Claros Avenida Professor Rui Braga S/NMontes ClarosMG39401-089Brazil Universidade Federal de Lavras Campus UniversitárioCP 3037LavrasMG37299-000Brazil

Background: This study aimed to determine the litterfall production, accumulation, decomposition rate and nutrient stocks, and to estimate the soil carbon(C) and nitrogen(N) stocks in three palm swamp ecosystems with different land use intensities in the Southeast of ***: Three palm swamp ecosystems with different land use intensities were evaluated: Agua Doce(AD),conserved area;Capivara(CV), area with small agricultural practices;and Buriti Grosso(BG), area with an intensive land use. Produced and accumulated litterfall from the Cerrado areas surrounding the palm swamps were collected from October 2014 to September 2015, and the Ca, Mg, K, and P concentrations were determined in the dry and rainy seasons. Soil samples were collected in the surrounding Cerrado and within the palm swamps until 100 cm soil depth to determine bulk density and soil C and N contents and ***: Annual litterfall production in the Cerrado surrounding palm swamps was similar in AD(3.58 Mg·ha^-1·year^-1) and CV(3.79 Mg·ha^-1·year^-1), and was lower in BG(2.84 Mg·ha^-1·year^-1), and was more intense during the dry season. Furthermore, litterfall accumulation was higher in CV(7.12 Mg·ha^-1·year^-1) and BG(6.75 Mg·ha^-1·year^-1), culminating in lower decomposition rates. AD showed the highest decomposition rate(0.60) due to its vegetation structure and conservation. The macronutrient contents from the production and accumulated litterfall had a low influence of the land use, decreasing in the following order: Ca > K > Mg > P. Soils in CV and BG palm swamp areas showed higher values of C and N contents, due to the use of land for agricultural crops and cattle raising in an extensive(rangeland) system. The BG palm swamp, showed the highest C(124.03 Mg·ha^-1) and N(10.54 Mg·ha^-1) stocks due to the land use history with different agricultural practices over ***: The litterfall dynamics in the Cerrado surrounding palm swamps was more affected by climatic variables t

关键词： Nutrient cycling Soil fertility Soil organic matter Decomposition rate