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A Plant SMALL RNA-BINDING PROTEIN 1 Family Mediates Cell-to-Cell Trafficking of RNAi Signals

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Molecular Plant 2020年第2期13卷 321-335页

作者： Yan Yan Byung-Kook ham Yee Hang Chong Shyi-Dong Yeh William j.Lucas Department of Plant Biology College of Biological SciencesUniversity of CaliforniaDavisCA 95616USA Department of Plant Pathology National Chung-Hsing UniversityTaichung Present address:Global Institute for Food Security University of SaskatchewanSaskatoonSK S7N 4J8Canada Present address:Department of Biology University of Saskatchewan112 Science PlaceSaskatoonSK S7N 5E2Canada

In plants,RNA interference(RNAi)plays a pivotal role in growth and development,and responses to environmental inputs,including pathogen *** intercellular and systemic trafficking of small interfering RNA(siRNA)/microRNA(miRNA)is a central component in this regulatory ***,little is known with regards to the molecular agents involved in the movement of these si/*** address this situation,we employed a biochemical approach to identify and characterize a conserved SMALL RNA-BINDING PROTEIN 1(SRBP1)family that mediates non-cell-autonomous small RNA(sRNA)*** Arabidopsis,AtSRBP1 is a glycine-rich(GR)RNA-binding protein,also known as AtGRP7,which we show binds single-stranded siRNA.A viral vector,Zucchini yellow mosaic virus(ZYMV),was employed to functionally characterized the AtSRBP1-4(AtGRP7/2/4/8)RNA recognition motif and GR ***-based studies revealed the GR domain as being necessary and sufficient for SRBP1 cell-to-cell *** together,our findings provide a foundation for future research into the mechanism and function of mobile sRNA signaling agents in plants.

关键词： RNAi small RNA-binding protein family small RNA movement non-cell-autonomous protein glycinerich protein

Plant lncRNAs are enriched in and move systemically through the phloem in response to phosphate deficiency

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j.urnal of Integrative Plant Biology 2019年第4期61卷 492-508页

作者： Zhaoliang Zhang Yi Zheng Byung-Kook ham Shupei Zhang Zhangj.n Fei William j.Lucas State Key Laboratory of Tea Biology and Utilization Anhui Agricultural University Boyce Thompson Institute for Plant Research Cornell University Global Institute for Food Security Department of Biology University of Saskatchewan Department of Plant Biology College of Biological Sciences University of California

In response to phosphate(Pi) deficiency, it has been shown that micro-RNAs(miRNAs) and mRNAs are transported through the phloem for delivery to sink tissues. Growing evidence also indicates that long noncoding RNAs(lncRNAs) are critical regulators of Pi homeostasis in plants. However, whether lncRNAs are present in and move through the phloem, in response to Pi deficiency, remains to be established. Here, using cucumber as a model plant, we show that lncRNAs are enriched in the phloem translocation stream and respond,systemically, to an imposed Pi-stress. A well-known lncRNA, IPS1, the target mimic(TM) of miRNA399,accumulates to a high level in the phloem, but is not responsive to early Pi deficiency. An additional 24 miRNA TMs were also detected in the phloem translocation stream; among them miRNA171 TMs and miR166 TMs were induced in response to an imposed Pi *** studies identified 22 lncRNAs which move systemically into developing leaves and root tips. A CU-rich PTB motif was further identified in these mobile lncRNAs. Our findings revealed that lncRNAs respond to Pi deficiency, non-cell-autonomously, and may act as systemic signaling agents to coordinate early Pi deficiency signaling, at the whole-plant level.

关键词： Plant lncRNAs enriched phloem phosphate deficiency

Enhancing the efficiency and scalability of perovskite solar cells through pseudo-halide salt addition

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j.urnal of Materials Science & Technology 2023年第34期165卷 161-169页

作者： Dohun Baek Geon Yeong Park j.ongbeom Cha Hyemi Na Dong Seok ham Min Kim School of Chemical Engineering Future Energy Research CenterJeonbuk National UniversityJeonju 54896Republic of Korea Graduate School of Integrated Energy-AI Jeonbuk National UniversityJeonju 54896Republic of Korea Chemical Materials Solutions Center Korea Research Institute of Chemical Technology(KRICT)Daejeon 34114Republic of Korea

Organic-inorganic halide perovskites have significant potential for application in next-generation solar ***,their applications are limited by challenges associated with large-area processability and long-term stability owing to the presence of pinholes and defect ***,we incorporated cesium formate in a perovskite active layer using a sequential perovskite fabrication process to eliminate the defect sites of perovskites and improve the film processability via roll-to-roll(R2R)*** addition of cesium formate salt to the PbI2 layer influences the perovskite crystal formation behavior as well as increases the perovskite crystallinity and decreases the defect ***,cesium formate addition eliminated small PbI2 grains and smoothed the perovskite surface,resulting in a large crystal grain *** formate ions interact with the PbI2 component and passivate halide vacancy defects,reducing nonradiative recombination and improving charge ***,the treated perovskite films were highly stable in air,exhibiting improved efficiency retention over time and achieving a power conversion efficiency(PCE)of>22%compared to the ***,the additive-treated perovskite film was processed using the R2R method,achieving a high PCE of>14%with minimal hysteresis.

关键词： Perovskite Solar Cells Pseudo-halide ion Stability Roll-to-roll process

Bowman layer transplantation in the treatment of keratoconus

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Eye and Vision 2018年第1期5卷 217-222页

作者： Diana C.Dragnea Rénuka S.Birbal Lisanne ham Isabel Dapena Silke Oellerich Korine van Dij. Gerrit R.j.Melles Netherlands Institute for Innovative Ocular Surgery(NIIOS) Laan op Zuid 883071AA RotterdamThe Netherlands Melles Cornea Clinic RotterdamThe Netherlands. Amnitrans EyeBank RotterdamThe Netherlands NIIOS-USA San DiegoUSA

Several treatment options corresponding to the grade of keratoconus have been *** are ultra-violet corneal crosslinking and intracorneal ring segments for mild to moderate keratoconus,and penetrating keratoplasty or deep anterior lamellar keratoplasty for the more advanced cases of *** layer transplantation was developed as a procedure for patients with advanced,progressive *** technique consists of transplanting an isolated donor Bowman layer into a mid-stromal pocket of a keratoconic cornea resulting in corneal flattening and stabilization against further ***,it aims at corneal stabilization in eyes with advanced keratoconus,and enabling continued contact lens wear for normal visual *** being a sutureless procedure and using an acellular graft,it potentially avoids commonly known suture and graftrelated complications of penetrating or deep anterior lamellar *** treatment seems to be a promising option in the management of advanced keratoconus in order to postpone or prevent a more invasive corneal surgery,while minimizing the risk of complications and allowing less stringent surveillance and less intensive medical therapy.

关键词： Cornea Advanced keratoconus Bowman layer Penetrating keratoplasty Deep anterior lamellar keratoplasty Crosslinking Intracorneal ring segments

Equilibria of Al-N and Nb-N in Molten Iron

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j.urnal of Iron and Steel Research International 1994年第1期1卷 7-12页

作者： CHEN Xiao Yi, ham Qi Yong, SOMG Bo and WAHG Rong Zhu University of Science & Technology Beij.ng

The equilibria of Al-N and Nb-N in molten iron at 1600℃ to 1700℃ by using of samplingmethod have been conducted. The thermodynamic parameters obtained areFor the reaction of 1/2N_2=[N] , lgK=-1106/T-0. 771lgK_(AiN) =- 9909/T + 3. 81=- 1 89800- 73.0/T j.mol, =- 0. 530+ 936/TlgK_(NbN)= - 34700/T+ 1 8. 37= - 664650- 352T j.mol, =- 0. 090- 258. 8/T

关键词： liquid iron Al-N equilibrium Nb-N equilibrium thermodynamic parameters

Role of E3 ubiquitin ligases in lung cancer

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World j.urnal of Clinical Oncology 2013年第3期4卷 58-69页

作者： Barbara C Snoek Leonie ham de Wilt Gerrit j.nsen Godefridus j.Peters Department of Medical Oncology VU University Medical Center1081 HV AmsterdamThe Netherlands Department of Rheumatology VU University Medical Center1081 HV AmsterdamThe Netherlands

E3 ubiquitin ligases are a large family of proteins that catalyze the ubiquitination of many protein substrates for targeted degradation by the 26S ***,E3 ubiquitin ligases play an essential role in a variety of biological processes including cell cycle regulation,proliferation and apoptosis.E3 ubiquitin ligases are often found overexpressed in human cancers,including lung cancer,and their deregulation has been shown to contribute to cancer ***,the lack of specific inhibitors in clinical trials is a maj.r issue in targeting E3 ubiquitin ligases with currently only one E3 ubiquitin ligase inhibitor being tested in the clinical *** this review,we focus on E3 ubiquitin ligases that have been found deregulated in lung ***,we discuss the processes in which they are involved and evaluate them as potential anti-cancer *** better understanding the mechanisms by which E3 ubiquitin ligases regulate biological processes and their exact role in carcinogenesis,we can improve the development of specific E3 ubiquitin ligase inhibitors and pave the way for novel treatment strategies for cancer patients.

关键词： E3 ubiquitin ligases Lung cancer Ubiquitinproteasome system Proteasome inhibitors Bortezomib Apoptosis Gene regulation DNA repair

The effects of selection for cold tolerance on cross-tolerance to other environmental stressors in Drosophila melanogaster

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Insect Science 2009年第3期16卷 263-276页

作者： Heath A. MacMillan j.llian P. Walsh Brent j. Sinclair Department of Biology The University of Western Ontario London ON N6A 5B7 Canada

Cross tolerance, whereby tolerance to one environmental stress is correlated with tolerance to other stressors, is thought to be widespread in insects. We used lines of Drosophila melanogaster Meigen （Diptera： Drosophilidae） selected for survival at a 1-h exposure to -5℃ to examine the extent to which this selection results in increased tolerance to other stresses, including high and low temperatures, desiccation and starvation. While selection improved tolerance to acute cold exposure and survival at -5℃, there was little effect of selection regime on tolerance to other stressors. There was no correlation between tolerances to any of the stressors, suggesting different mechanisms of tolerance. This supports arguments that correlations between stress tolerances during selection experiments with D. melanogaster may be coincidental. The magnitude of heat-hardening was apparently constrained by basal tolerance among lines, but the magnitude of the rapid cold-hardening response was not correlated with basal cold tolerance, implying that the relationship between inducible and basal tolerances differs at high and low temperatures.

关键词： chill coma cold tolerance cross-tolerance desiccation heat shock high temperature laboratory selection longevity rapid cold hardening starvation

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标准电池大组的内部比较

中国检验检测 1975年第3期 44-47页

作者： j.Wilbur-ham 杨华山袁杰

一、引言一组标准电池组成实验室的电动势标准。当它们保存在恒温环境下时,它们的短期稳定性确是很好的。它们的长期稳定性并不是这样好的,其中的一些电池被观察到有这样或那样的轻微漂移。除非它们全部作同样漂移,有理由认为电池组的... 详细信息

一、引言一组标准电池组成实验室的电动势标准。当它们保存在恒温环境下时,它们的短期稳定性确是很好的。它们的长期稳定性并不是这样好的,其中的一些电池被观察到有这样或那样的轻微漂移。除非它们全部作同样漂移,有理由认为电池组的平均值

关键词：残差差值剩余误差微伏相对值误差分量均方根误差最小二乘法最小二乘算法计算方法电池组内部比较

Manual mid-stromal dissection as a low risk procedure to stabilize mild to moderate progressive keratoconus

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Eye and Vision 2018年第1期5卷 233-239页

作者： Rénuka S Birbal Korine van Dij. j.ck S Parker Henny Otten Maha Belmoukadim Lisanne ham Lamis Baydoun Isabel Dapena Gerrit R j.Melles Netherlands Institute for Innovative Ocular Surgery(NIIOS)Rotterdam Laan op Zuid 883071AA RotterdamThe Netherlands Melles Cornea Clinic Rotterdam RotterdamThe Netherlands Amnitrans EyeBank Rotterdam RotterdamThe Netherlands. NIIOS-USA San DiegoUSA Parker Cornea BirminghamALUSA Visser Contact Lens Practice Nij.egen/Rotterdam RotterdamThe Netherlands

Background:To evaluate the efficacy of manual mid-stromal dissection in stabilizing progressive ***:Surgeries were performed in 16 eyes of 14 patients with progressive *** eyes were examined before and at 1 day,1 week,1,3,6 and 12 months after surgery,and every 6 months ***(simK,Kmax and pachymetry),best corrected visual acuity(BCVA)and subj.ctive refraction were recorded up to the latest follow-up visit(mean follow-up time 6.6±2.4 years).Results:All surgeries were uneventful,and no postoperative complications *** values(n=15)stabilized in 6/11 eyes(55%)with a preoperative Kmax60 D showed continued *** 11/15 eyes(73%)pachymetry was *** with spectacles remained stable in 7/12 eyes(58%)and improved≥2 Snellen lines in 5/12 eyes(42%).BCVA with a contact lens remained stable in 4/9 eyes(44%),improved≥2 Snellen lines in 3/9 eyes(33%)and deteriorated in 2/9 eyes(22%).Conclusions:Manual mid-stromal dissection was effective in 50%of keratoconic corneas with Kmax values<60 D and may be considered in cases ineligible for other interventions such as UV-crosslinking,stromal ring implantation or Bowman layer *** advantage of the procedure may be that the tissue is unaltered and that no synthetic or biological implant is required.

关键词： Keratoconus Manual cornea dissection Progressive ectasia Surgical technique