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Bee Pollen in Transylvania （Romania）： Palynological Characterization and ORACFL Values of Lipophilic and Hydrophilic Extracts of Monofloral Pollen Pellets

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Journal of Agricultural Science and Technology(A) 2016年第1期6卷 18-37页

作者： Oltica Giorgiana Stanciu Daniel Severus Dezmirean maria graca campos Life Science Institute Department of Animal Science Technologies University of Agricultural Sciences and Veterinary Medicine3-5 Manastur Street Cluj-Napoea 400372 Romania Coimbra Chemistry Centre (CQC FCT Unit 313) and Laboratory of Pharmacognosy Faculty of Pharmacy University ofCoimbra Polo III Azinhaga De Santa Coimbra Coimbra 3000-3701 Portugal

A melissopalynological study carried out on 35 bee pollen samples from Romania （Transylvania） shows predominant species as Taraxacum officinale, Tilia sp., Ferbascum sp., Zea mays L., Onobrychis viciifolia Scop., Geranium sanguineum L., Filipendula ulmaria L., Cydonia oblonga L., Calluna vulgaris L. and Brassica sp.. This is the first time for the contribution of total phenolic （TP） and total carotenoid （TC） content to the modified oxygen radical absorbance capacity （ORACFL） values in 29 monofloral samples. Hydrophilic ORACFL （H-ORACFL） values ranged between 7.13-10.12 μmol TE/g and 6.94-22.46 μmol TE/g, whereas lipophilic ORACFL （L-ORACFL）values were between 2.19-7.79 μmol TE/g and 1.65-9.96 μmol TE/g, in multifloral and monofloral samples, respectively. It has proved the complex involvement of botanical origins on the antioxidant features with a specific occurrence. Some monofloral samples presented particular high antioxidant potential, such as Salix sp., Taraxacum officinale, Matricaria chamomilla, Cichorium intybus and the poorly studied Cirsium arvense （L.） Scop. and Scilla bifolia L.. New variables, such as soil characteristics, climates together with botanical origins, were introduced in a multivariate analysis of antioxidant data matrix, given a possible important involvement of all of them in affecting, not only the phytochemical composition, but thereafter the antioxidant capacity. All these data could be crucial to a new way of gathering, by beekeepers, depending on the market demand and the purposes for the product that has a potential for further therapeutic bioactivities investigations, and added value in the enrichment of certain products.

关键词： Pollen ORAC antioxidant flavonoids carotenoids.

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Boron neutron capture therapy of cancer:where do we stand now?

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Cancer Communications 2024年第8期44卷 889-892页

作者： Rolf F.Barth Gong Wu maria da graca H.Vicente John C Grecula Nilendu Gupta Department of Pathology The Ohio State UniversityColumbusOhioUSA Mass Spectrometry and Proteomics Facility The Ohio State UniversityColumbusOhioUSA Department of Chemistry Louisiana State UniversityBaton RougeLouisianaUSA Department of Radiation Oncology The Ohio State UniversityColumbusOhioUSA

1 INTRODUCTION,This is the third Editorial/Commentary that one of us(***)has written relating to boron neutron capture therapy(BNCT)[1,2].For those readers who are unfamiliar with BNCT we would refer them to several recent comprehensive reviews[3–5].The second Editorial ended on a hopeful note that with the introduction of acceleratorbased neutron sources(ABNSs),BNCT would enter into the mainstream of radiation therapy[2].

关键词： boron 1,2 neutron

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Zea mays L. Pollen： An Approach to Its Quality Control

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Journal of Agricultural Science and Technology(B) 2015年第8期5卷 513-522页

作者： maria graca campos Norma Almaraz-Abarca] Miguel Pires Matos Nelson Mortagua Gomesl VanildaAparecida Arruda Ortrud Monika Barth Alex Silva Freitas D6bora Cristina Amancio' Ligia Bicudo Almeida-Muradian Coimbra Chemistry Center Faculty of Sciences and Technology and Faculty of Pharmacy University of Coimbra Polo 111Azinhaga de Santa Comba Coimbra 3000-548 Portugal Interdisciplinary Research Centre for Integral Regional Development-Durango National Polytechnic Institute Sigma 119 20 deNoviembre 11 Durango 34220 DGO Mexico Department of Food and Experimental Nutrition Pharmaceutical Science School University of S6o Paulo Av. Prof. Lineu Prestes580 Bloco 14 CEP055800-000 Sao Paulo Brazil Instituto Oswaldo Cruz Fundaao Oswaldo Cruz Avenida Brasi14365 Rio de Janeiro 21040-900 Brazil

Zea mays L. is one of the biggest cropping systems among the sustainable development agronomy. Pollen from this crop source is unexplored and apiculture can be a good partner adding value to the product and creating new jobs helping to solve some social issues as unemployment. However, food safety is crucial, thus the aim of this study was to explore the flavonoid/phenolic profiles from Z. mays L. pollen as a fingerprint for this plant identification and also to demonstrate how the method of bee pollen samples （honeybee collected pollen） is applied. For this purpose, several sources ofZ. mays L. pollen were analyzed, including corn hybrids and genetic modified samples collected at the breeding fields. For this work, samples were taken at several years from 2000 to 2012 and collected from different countries and locations, such as Portugal, Mexico and Brazil. Results showed, for the first time, that the fingerprint （flavonoid/phenolic profile） for Z. mays L. pollen does not change over the time of sampling neither with the region of harvesting. The high performance liquid chromatography-diode array detector （HPLC/DAD） fingerprints of phenolic/flavonoid extract from Z. mays remain unchanged for all samples analyzed from different countries, hybrids and/or genetic modified plants. This is also the first study reporting these phenolic compounds not only in pollen collected directly from hybrid plants, but also in Z. mays bee pollen. The described fingerprinting method is easy, fast and accurate for the characterization of Z. mays L. pollen samples and complete microscopic analysis because it is species-specific.

关键词： Flavonoids phenols Zea mays pollen bee pollen food safely phenolic fingerprinting palynological analysis oxygenradical absorbance capacity (ORAC).

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Mapping the clinical practice of traditional,complementary and integrative medicine in oncology in Western countries:A multinational cross-sectional survey

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Journal of Integrative Medicine 2024年第1期22卷 64-71页

作者： Matthias Huemer Sandro graca Sarah Bitsche Guenter Hofmann Mike Armour Martin Pichler Palliative Care Unit Division of OncologyDepartment of Internal MedicineMedical University of Graz8036 GrazAustria Northern College of Acupuncture York YO16LJUnited Kingdom School of Health and Society Faculty of EducationHealth and WellbeingUniversity of WolverhamptonWolverhampton WV11LYUnited Kingdom National Institute of Complementary Medicine Health Research Institute Western Sydney UniversityPenrith NSW 2751Australia Translational Health Research Institute Western Sydney UniversityPenrith NSW 2751Australia Medical Research Institute of New Zealand Wellington 6021New Zealand Translational Oncology University Hospital of Augsburg86156 AugsburgGermany

Background Many cancer patients seek adjunctive therapies to biomedical cancer treatments at some point of their disease *** acupuncture is increasingly recommended by leading oncological associations,limited evidence exists concerning the evidence-informed practice and adherence to current guidelines of traditional complementary and integrative medicine(TCIM)practitioners treating cancer *** An international online-survey assessed the demographical data,clinical practice,and sources of information used by TCIM practitioners in Austria,Germany,United States of America,Australia,and New *** In total,404 respondents completed the survey,of which 254(62.9%)treated cancer *** practitioners were acupuncturists and herbalists(57.1%),had(16.8±9.9)years of clinical experience and see a median of 2(1,4)cancer patients per *** cancer(61.8%)is the most common cancer type seen in TCIM *** TCIM treatments are frequently concurrent with the patient’s cancer specific treatment(39.9%),which is also reflected by the main goal of a TCIM treatment to alleviate side effects(52.4%).However,only 28.0%of the respondents are in contact with the treating *** to the respondents,pain is most effectively treated using acupuncture,while herbal medicine is best for cancer-related *** practitioners mostly use certified courses(33.1%)or online databases(28.3%)but often believe that experts are more reliable to inform their practice(37.0%)than research publications(32.7%).Conclusion Acupuncturists and herbalists commonly treat cancer *** practitioners use TCIM as an adjunct to biomedicine as supportive care and use it largely in accordance with current oncological guidelines.

关键词： Integrative medicine Acupuncture Oncology Evidence-informed practice International survey

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Iron overload and immunity

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World Journal of Gastroenterology 2007年第35期13卷 4707-4715页

作者： graca Porto maria De Sousa ICBAS Abel Salazar Institute for the Biomedical SciencesPortoIBMCInstitute of Molecular and Cellular BiologyPortoPortugal HGSASanto António General HospitalPorto 823 4150Portugal ICBAS Abel Salazar Institute for the Biomedical SciencesPortoIBMCInstitute of Molecular and Cellular BiologyPortoPortugal

Progress in the characterization of genes involved in the control of iron homeostasis in humans and in mice has improved the definition of iron overload and of the cells affected by it. The cell involved in iron overload with the greatest effect on immunity is the macrophage. Intriguing evidence has emerged, however, in the last 12 years indicating that parenchymal iron overload is linked to genes classically associated with the immune system. This review offers an update of the genes and proteins relevant to iron metabolism expressed in cells of the innate immune system, and addresses the question of how this system is affected in clinical situations of iron overload. The relationship between iron and the major cells of adaptive immunity, the T lymphocytes, will also be reviewed. Most studies addressing this last question in humans were performed in the clinical model of Hereditary Hemochromatosis. Data will also be reviewed demonstrating howthe disruption of molecules essentially involved in adaptive immune responses result in the spontaneous development of iron overload and how they act as modifiers of iron overload.

关键词： Iron Iron overload Innate immunity Adaptive immunity

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Effect of lead chloride on spermatogenesis and sperm parameters in mice

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Asian Journal of Andrology 2004年第3期6卷 237-241页

作者： António graca Joao Ramalho-Santos maria de Lourdes Pereira Department of Zoology University of CoimbraCoimbraPortugal Department of BiologyUniversity of Aveiro3810 AveiroPortugalDepartment of ZoologyUniversity of CoimbraCoimbraPortugalDepartment of BiologyUniversity of Aveiro3810 AveiroPortugal

Aim: To evaluate the effect of acute lead chloride exposure on testis and sperm parameters in mice. Methods: PbCl2, 74 mg/kg, was daily administered to sexually mature male mice for 3 days and the effects on the testicular histology and ultrastructure as well as the motility and density of spermatozoa in cauda epididymis were observed. An additional group of mice were treated for 1-3 days and were allowed to recover for 32 days to determine the reversibility of lead-induced changes. Results: The testicular weight, seminiferous tubular diameter and sperm counts were significantly decreased following 3 days of PbCl2 treatment, but were unaffected by shorter-term exposures. The changes caused by lead are mostly reversible. Conclusion: Acute lead chloride exposure injures the fertility parameters of male mice and the effects are partially reversible.

关键词： lead chloride seminiferous tubules epididymis semen analysis

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G protein-coupled estrogen receptor 1(GPER) activation triggers different signaling pathways on neurons and astrocytes

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Neural Regeneration Research 2019年第12期14卷 2069-2070页

作者： Claudio Roque graca Baltazar CICS-UBI - Health Sciences Research Centre University of Beira Interior Covilh. Portugal Faculty of Health Sciences University of Beira Interior Covilh. Portugal

Estradiol (E2) is the most potent and prevalent form of estrogen, a well-known hormone that regulates multiple tissues and functions in humans. In the brain, E2 regulates processes as diverse as learning, memory, cognition, mood, as well as neurodevelopment and neurodegeneration. The actions of E2 are mediated by classical estrogen receptors (ERs;α and β), and by the G protein-coupled estrogen receptor 1 (GPER or GPR30)(Prossnitz and Arterburn, 2015). Classical ER are predominantly present in the nucleus and cytoplasm, with less than 2% present on the plasma membrane, and mediate genomic cellular effects that occur in the time frame of hours to days (Prossnitz and Arterburn, 2015).

关键词： multiple tissues functions astrocytes

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DNA Compaction and Decompaction:Bulk and Interfaces

DNA Compaction and Decompaction:Bulk and Interfaces

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中国化学会第十届胶体与界面化学会议

作者： maria da graca Miguel Chemistry Department Coimbra University3004-535 CoimbraPortugal

＜正＞ Polyvalent cationic cosolutes induce DNA complexation,compaction and *** physico-chemicalresearch on various mixed systems of DNA and cationic amphiphiles and polyions in bulk and at interfaces will *** of DNA with simple cationic surfactants will be reviewed,followed by DNA interaction withcationic-anionic *** also include interaction with thermodynamically stable catanionic vesicles,built onclassical and aminoacid-based *** phase map determinations and cryo-transmission electronmicroscopy,an overview from phase behaviour to microstructure will be presented for several *** will alsoaddress DNA compaction and decompaction phenomena in different mixed systems,and their dependence on theamphiphile hydrophobicity and mixture.

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Cloud of strings in f(R) gravity

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Chinese Physics C 2018年第6期42卷 44-52页

作者： J.P.Morals graca Iarley P.Lobo Ines G.Salako Departamento de Fisica Universidade Federal do ParanaCuritibaPRBrazil Departamento de Fisica Universidade Federal da ParafbaCaixa Postal 5008CEP 58051-970Joao PessoaPBBrazil Institut de Mathematiques et de Sciences Physiques(IMSP)01 BP 613 Porto-Novo Benin

We derive the solution for a spherically symmetric string cloud configuration in a ＆dimensional spacetime in the framework of f（R） theories of gravity. We also analyze some thermodynamic properties of the joint black hole - cloud of strings solution. For its Hawking temperature, we found that the dependence of the mass with the horizon is significantly different in both theories. For the interaction of a black hole with thermal radiation, we found that the shapes of the curves are similar, but shifted. Our analysis generalizes some known results in the literature.

关键词： modified theories of gravity black hole thermodynamics gravitational aspects of string theory

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Scalar resonant frequencies and Hawking effect of an f(R) global monopole

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Chinese Physics C 2017年第9期41卷 138-145页

作者： H. S. Vieira J. P. Morals graca V. B. Bezerra Institute of Cosmology Department of Physics and Astronomy Tufts University Medford Massachusetts 02155 USA Departamento de Fisica Universidade Federal da Parafba Caixa Postal 5008 CEP 58051-970 Joao Pessoa PB Brazil Instituto de Fisica Universidade Federal do Parana Curitiba PR Brazil

Massive scalar fields are considered in with a global monopole in f（R） gravity. The exact background is obtained and is given in terms of the Heun functions to study the Hawking radiation and the gravitational field produced by a Schwarzschild black hole solution of the radial part of the Klein-Gordon equation in this general Heun functions. We apply the properties of the general the resonant frequencies of scalar particles.

关键词： massive scalar field general Heun function black hole radiation energy level

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