检索结果-南通市图书馆

High-level expression of recombinant IgG1 by CHO K1 platform

在线全文

学校读者我要写书评

暂无评论

Frontiers of Chemical Science and Engineering 2015年第3期9卷 376-380页

作者： Ningning Xu Jianfa Ou Al-Karim (AI) Gilani Lufang Zhou margaret Liu Department of Chemical and Biological Engineering The University of Alabama Tuscaloosa AL 35294 USA Departments of Medicine and Biomedical Engineering The University of Alabama at Birmingham Birmingham AL 35294 USA

The Chinese Hamster Ovary （CHO K1） cell was used to express a targeted anti-cancer monoclonal antibody by optimizing the platform of the construction of production cell line in this study. The adherent CHO K1 was first adapted to suspension culture in chemical defined medium. Then the glutamine synthetase （GS） vector was applied to construct a single plasmid to overexpress a monoclonal antibody IgG1. Post transfection, the produc- tion of cell pool was optimized by glutamine-free selection and amplification using various concentrations of methio- nine sulfoximine. The best cell pool ofCHO K1/IgG1 was used to screen the top single clone using the limiting dilution cloning. Finally, a high IgG1 production of 780 mg/L was obtained from a batch culture. This study demonstrated that the construction of high producing cell line, from gene to clone, could be completed within six month and the gene amplification improved protein production greatly.

关键词： Chinese hamster ovary (CHO) monoclonal antibody IgG1 amplification cell line development

Feasibility of sustaining the upland conversion program by establishing a domestic carbon market

在线全文

学校读者我要写书评

暂无评论

Forestry Studies in China 2008年第4期10卷 205-211页

作者： WANG Zan-xin margaret M. CALDERON School of Development Studies Yunnan University Kunming 650091 E R. China College of Forestry and Natural Resources University of the Philippines Los Banos Laguna 4031 Philippines

To sustain the upland conversion program （UCP） in China after the government compensation expires, we suggest an establishment of a domestic carbon market where forest carbon from the UCP can be traded. Taking southwest China＇s Yunnan Province as an example, we explored the feasibility of switching the UCP to a carbon offset project. The breakeven carbon price which is equivalent to the opportunity cost of agricultural cultivation was estimated and then compared with the carbon price in the international market. We found that it is feasible to change the UCP to a carbon offset project if the duration is longer than 10 years at a discount rate of 7%, and if the recent bid price （147.2 Yuan.t-1） for Chinese carbon offset project prevails. The feasibility is better for converted land with lower productivity when the project duration is given. For a given site index, the feasibility is lower as pro- ject length is reduced. The results of sensitivity analysis show that the feasibility will be enhanced as the discount rate increases; however, the changes in the price of agricultural products and the amount of sequestered carbon have insignificant effects on the choice of sites and project duration.

关键词： upland conversion program carbon sequestration domestic carbon market

Systematic review of novel ablative methods in locally advanced pancreatic cancer

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2014年第9期20卷 2267-2278页

作者： margaret G Keane Konstantinos Bramis Stephen P Pereira Giuseppe K Fusai Institute for Liver and Digestive Health University College London Royal Free Campus London NW3 2PF United Kingdom HPB and Liver Transplant Unit Royal Free Hospital London NW3 2QG United Kingdom

Unresectable locally advanced pancreatic cancer with or without metastatic disease is associated with a very poor *** standard therapy is limited to chemotherapy or *** regimens have been shown to have a substantial survival advantage and novel treatment strategies are urgently *** and laser based ablative techniques are widely used in many solid organ *** studies in the pancreas were associated with significant morbidity and mortality,which limited widespread *** to the various applications,in particular combining the techniques with high quality imaging such as computed tomography and intraoperative or endoscopic ultrasound has enabled real time treatment monitoring and significant improvements in *** conducted a systematic review of the litera-ture up to October *** studies suggest that ablative therapies may confer an additional survival benefit over best supportive care but randomised studies are required to validate these findings.

关键词： Pancreatic cancer Radiofrequency ablation Photodynamic therapy Cryoablation Microwave ablation High frequency focused ultrasound Irreversible electroporation

Drosophila ubiquitin E3 ligase dSmurf is required for synapse remodeling and axon pruning by glia

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2017年第1期44卷 67-70页

作者： Changyan Chen Shuai Yin Wenze Cao margaret S. Ho Institute of Intervention Vessel Shanghai 10th People's HospitalShanghai Key Laboratory of Signaling and Diseases Research School of Life Science and Technology Tongji University 1239 Siping Road Shanghai 200092 China Research Center for Translational Medicine Shanghai 250092 China Key Laboratory of Arrhythmias of the Ministry of Education of China Shanghai East Hospital No. 150 Jimo Road Shanghai 200092 China Department of Anatomy and Neurobiology 1239 Siping Road Tongji University School of Medicine Shanghai 200092 China

Animal behaviors and higher-order functions rely on complex neural circuits built by synaptic connections （synapses） to deliver messages among different brain cells. As the major mediator in the nervous system, neur... 详细信息

关键词： Drosophila ubiquitin E3 ligase dSmurf is required for synapse remodeling and axon pruning by glia

鸡蛋中全氟辛烷磺酸以及相关全氟化合物的污染现状

在线全文

学校读者我要写书评

暂无评论

科学通报 2008年第2期53卷 147-152页

作者：王媛杨伟贤山下信义谷保佐知苏敏嘉 margaret B.murphy 林群声香港城市大学生物化学系海洋污染和保护研究中心香港 National Institute of Advanced Industrial Science and Technology (AIST) 16-10nogawa Tsukuba Ibaraki 305-8569 Japan

目前环境中全氟有机化合物(PFCs)的污染引起了人们的广泛关注.近期的研究发现,中国人血中全氟辛烷磺酸(PFOS)的浓度明显高于其他国家.本研究的目的是:(1)检测中国市场鸡蛋中PFCs的浓度和组成;(2)对人类健康进行初步的风险评价.采集于中... 详细信息

目前环境中全氟有机化合物(PFCs)的污染引起了人们的广泛关注.近期的研究发现,中国人血中全氟辛烷磺酸(PFOS)的浓度明显高于其他国家.本研究的目的是:(1)检测中国市场鸡蛋中PFCs的浓度和组成;(2)对人类健康进行初步的风险评价.采集于中国8个地区的鸡蛋样品用来检测11个全氟有机化合物.结果显示蛋黄几乎100%含有PFOS,而蛋白里未检测到(<0.08ng/g(湿重)).在全氟烷基磺酸盐(perfluoroalkylsulfonates)中,只有PFOS在所有鸡蛋中被检测出.而在全氟烷基羧酸(perfluoroalkycarboxylates)中,全氟十一酸(perfluoroundecanoic acid,PFUnDA)在所有鸡蛋中被检测出,全氟辛酸(perfluorooctanoicacid,PFOA)和全氟癸酸(perfluorodecanoic acid,PFDA)分别在75%和50%的鸡蛋样品中被检测出.鸡蛋中PFOS的浓度范围是45.0～86.9ng/g(湿重).风险评价结果显示,目前中国鸡蛋中PFOS的浓度不会对人类产生即时危害。

关键词： PFOS 鸡蛋人类健康风险评价蛋黄蛋白

Entanglement and nonlocality in multi-particle systems

在线全文

学校读者我要写书评

暂无评论

Frontiers of physics 2012年第1期7卷 72-85页

作者： margaret D. Reid (1) Qiong-Yi He (1) Peter D. Drummond (1) 1. ARC Centre of Excellence for Quantum-Atom Optics Centre for Atom Optics and Ultrafast Spectroscopy Swinburne University of Technology Melbourne 3122 Australia

Entanglement, the Einstein-Podolsky Rosen （EPR） paradox and Bell＇s failure of local-hidden- variable （LHV） theories are three historically famous forms of ＂quantum nonlocality＂. We give experimental criteria for these three forms of nonlocality in multi-particle systems, with the aim of better understanding the transition from microscopic to macroscopic nonlocality. We examine the nonlocality of N separated spin J systems. First, we obtain multipartite Bell inequalities that address the correlation between spin values measured at each site, and then we review spin squeezing inequal- ities that address the degree of reduction in the variance of collective spins. The latter have been particularly useful as a tool for investigating entanglement in Bose Einstein eondensates （BEC）. We present solutions for two topical quantum states： multi-qubit Greenberger-Horne Zeilinger （GHZ） states, and the ground state of a two-well BEC.

关键词： entanglement quantum nonlocality multi-particle two-well Bose-Einstein condensates(BEC)

How does the tea L-theanine buffer stress and anxiety

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2022年第3期11卷 467-475页

作者： Liwen Wang margaret Brennan Shiming Li Hui Zhao Klaus WLange Charles Brennan College of Food Science and Technology Hebei Agricultural UniversityBaoding 071001China Department of Wine Food and Molecular BiosciencesLincoln UniversityChristchurch 7647New Zealand Hubei Key Laboratory of EFGIR Huanggang Normal UniversityHuanggang 438000China Tianjin Key Laboratory of Food and Biotechnology University of CommerceTianjin 300134China Department of Experimental Psychology University of RegensburgRegensburg 93040Germany School of Science RMITMelbourneVIC 3000Australia

Tea(Camellia sinensis)is widely considered to promote feelings of calming and *** effect is attributed to L-theanine(L-γ-glutamylethylamide)in tea,a non-protein amino acid mainly derived from tea *** a naturally occurring structural analogue of glutamate,L-theanine competes for the receptors with glutamate and is able to pass the blood-brain barrier to exert its relaxation *** review focuses on the relaxation effect of L-theanine,including animal models and the latest human trials as well as the potential molecular mechanisms regarding neuron stem *** biological efficacy of dietary L-theanine in the food matrix has been further discussed in this review in relation to the physiological changes in the gastrointestinal tract and bindings of L-theanine with other food components.

关键词： L-theanine Relaxation Stress Anxiety Food matrix

Developing Exon-Primed Intron-Crossing （EPIC） markers for population genetic studies in three Aedes disease vectors

在线全文

学校读者我要写书评

暂无评论

Insect Science 2015年第3期22卷 409-423页

作者： Vanessa Linley White Nancy margaret Endersby Janice Chan Ary Anthony Hoffmann Andrew Raymond Weeks Department of Genetics Bio21 Institute the University of Melbourne Victoria 3010 Australia

Aedes aegypti, Aedes notoscriptus, and Aedes albopictus are important vectors of many arboviruses implicated in human disease such as dengue fever. Genetic markers applied across vector species can provide important information on population structure, gene flow, insecticide resistance, and taxonomy, however, robust microsatellite markers have proven difficult to develop in these species and mosquitoes generally. Here we consider the utility and transferability of 15 Ribosome protein （Rp） Exon-Primed Intron- Crossing （EPIC） markers for population genetic studies in these 3 Aedes species. Rp EPIC markers designed for Ae. aegypti also successfully amplified populations of the sister species, Ae. albopictus, as well as the distantly related species, Ae. notoscriptus. High SNP and good indel diversity in sequenced alleles plus support for amplification of the same regions across populations and species were additional benefits of these markers. These findings point to the general value of EPIC markers in mosquito population studies.

关键词： Aedes aegypti Aedes albopictus Aedes notoscriptus Exon-Primed Intron- Crossing (EPIC) indels Ribosome protein (Rp) SNPs

Chylous ascites in colorectal surgery:A systematic review

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Surgery 2021年第6期13卷 585-596页

作者： Zi Qin Ng margaret Han Han Nien Beh Simon Keelan Department of General Surgery St John of God Midland HospitalMidland 6056Western AustraliaAustralia Department of General Surgery Joondalup Health CampusJoondalup 6027Western AustraliaAustralia

BACKGROUND Chylous ascites is a rare complication in colorectal surgery with limited *** To systematically review all available evidence to describe the incidence,clinical presentation,risk factors and management *** The systematic review was performed through PubMed,MEDLINE,EMBASE and Cochrane and cross-checked up to November *** data collated included:Demographics,indications(benign vs malignant),site of disease,surgical approach,extent of lymphadenectomy,day to and method of diagnosis of chylous ascites and management *** A total of 28 studies were included in the final analysis(426 cases).Patient age ranged from 31 to 89 *** except one case were performed for *** the 426 cases,195 were right-colonic,121 left-colonic,103 pelvic surgeries and 7 *** majority were diagnosed during the same inpatient stay by recognition of typical drain appearance and increased *** cases were diagnosed during outpatient visits with increased abdominal distention and subsequently underwent *** cases were managed successfully non-operatively(fasting with prolonged drainage,total parenteral nutrition,somatostatin analogues or a combination of these).Only three cases required surgical intervention after failing conservative management and subsequently resolved *** factors identified include:Right-colonic surgery/tumour location,extent of lymphadenectomy and number of lymph nodes *** Chylous ascites after colorectal surgery is a relatively rare *** the majority of cases resolved without surgical intervention,preventative measures should be undertaken such as meticulous dissection and clipping of lymphatics during lymphadenectomy to prevent morbidity.

关键词： Chylous Chyle Ascites Leak Colon Rectum Lymphadenectomy