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Evidence of endometrial amino acid metabolism and transport modulation by peri-ovulatory endocrine profiles driving uterine receptivity

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Journal of Animal Science and Biotechnology 2017年第4期8卷 852-865页

作者： Moana Rodrigues Franca maressa izabel santos da silva Guilherme Pugliesi Veerle Van Hoeck Mario Binelli Department of Animal Reproduction School of Veterinary Medicine and Animal ScienceUniversity of Sao Paulo225Duque de Caxias Norte Ave.Jd.Elite13635-900 PirassunungaSPBrazil Gamete Research Centre University of AntwerpAntwerpBelgium

Background: In beef cattle, changes in the periovulatory endocrine milieu are associated with fertility and conceptus growth. A large preovulatory follicle(POF) and the resulting elevated concentrations of progesterone(P4) during diestrus positively affect pregnancy rates. Amino acids(AA) are important components of maternally derived secretions that are crucial for embryonic survival before implantation. The hypothesis is that the size of the POF and the concentration of P4 in early diestrus modulate the endometrial abundance of SLC transcripts related to AA transport and metabolism and subsequently impact luminal concentrations of AA. The follicle growth of Nelore cows was manipulated to produce two experimental groups: large POF and CL(LF-LCL group) and small POF and CL(SF-SCL group). On day 4(D4; Experiment 1) and day 7(D7; Experiment 2) after Gn RH-induced ovulation(Gn RH treatment = D0), the animals were slaughtered and uterine tissues and uterine washings were collected. q RT-PCR was used to evaluate the expression levels of AA transporters in D4 and D7 endometrial *** concentrations of AA were quantified in D4 and D7 uterine washings by ***: Transcript results show that, on D4, SLC6 A6, SLC7 A4, SLC17 A5, SLC38 A1, SLC38 A7 and SCLY and on D7 SLC1 A4, SLC6 A1, SLC6 A14, SLC7 A4, SLC7 A7, SLC7 A8, SLC17 A5, SLC38 A1, SLC38 A7, SLC43 A2 and DDO were more abundant in the endometria of cows from the LF-LCL group(P dance of transcripts related to AA transport and metabolism in the endo

关键词： Amino acids Beef cattle Sex steroids Uterus

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Immune response to inactivated bacterial vector carrying the recombinant K39 antigen of Leishmania infantum in mice

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Asian Pacific Journal of Tropical Biomedicine 2024年第5期14卷 199-206页

作者： Lucelina S.Araújo Bruno B.silva Eduarda N.F.N.santos Arnaldo S.Bezerra Samuel S.Frota Assis R.Montenegro Eridan O.P.T.Florean Maurício Fvan Tilburg Maria izabel F.Guedes Laboratory of Biotechnology and Molecular Biology Northeast Biotechnology NetworkState University of CearáFortalezaBrazil Faculty of Veterinary Medicine State University of CearáFortalezaBrazil

Objective:To evaluate the immunological response elicited by an inactivated bacterial vector carrying the K39 antigen of Leishmania infantum,and a purified ***:Mice were subjected to the following treatments:(1)Purified recombinant K39(rK39)protein at a 20μg dose with complete Freund’s adjuvant;(2)Inactivated Escherichia coli(BL21 DE3)carrying the K39 protein at an equivalent total protein content of 200μg;(3)Inactivated bacteria lacking the K39 protein;(4)Non-immunized control *** monitoring was *** groups were challenged by intraperitoneal injection of 10^(7) Leishmania infantum *** euthanasia,the liver and spleen were collected to analyze the levels of TNF,IFN-γ,IL-12,IL-4,and ***:Mice immunized with purified rK39 or the inactivated bacterial vector carrying the K39 antigen of Leishmania infantum showed a long-lasting immune response with high levels of polyclonal antibodies specifically recognizing the recombinant *** IgG1 subclass was the predominant immunoglobulin;however,the induction of IgG2a and the profile of cytokines produced were indicative of the induction of a mixed-type ***:The inactivated bacterial vector carrying the K39 antigen,as well as the purified antigen can induce a long-lasting immune response in immunized mice,predominantly favouring a Th2 profile response.

关键词： Visceral leishmaniasis K39 Inactivated bacterial vector Vaccine Immune response Th1 Th2 Leishmania infantum

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In Silico and in Vitro Analysis of Pyrazolone Derivatives against Zika Virus and Identification of Potential NS5 Methyltransferase Inhibitors by Molecular Docking

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Journal of Biosciences and Medicines 2024年第7期12卷 214-229页

作者： Luciene Soares silva Tiago Soares Barbosa Maria Leonisa Sanchez-Nuñez Aldenise Mont Serrat Rosa da silva Vitor Won-Held Rabelo Leonardo dos santos Corrêa Amorim Leonardo Alves Miceli Percilene Fazolin Vegi Alice Bernardino Helena Carla Castro izabel Christina Nunes de Palmer Paixão Science and Biotechnology Graduate Program Department of Cellular and Molecular Biology Institute of Biology Fluminense Federal University Niteri Brazil Science and Biotechnology Graduate Program Department of Cellular and Molecular Biology Institute of Biology Fluminense Federal University Niterói Brazil Laboratory of Antibiotics Biochemistry Teaching and Molecular Modeling Institute of Biology Fluminense Federal University Niterói Brazil Laboratory of Organic Chemistry at the Institute of Chemistry Institute of Biology Fluminense Federal University Niterói Brazil

Zika virus (ZIKV), a mosquito-borne flavivirus, has been associated with benign infections for decades. However, it has become a public health concern due to its association with severe fetal and neurological complications. Although many efforts have been made to control ZIKV infection, approved vaccines or antiviral drugs are still lacking. Consequently, the development of new effective anti-ZIKV agents is urgently needed. In this context, we investigated the antiviral potential of pyrazolone derivatives against ZIKV replication using in silico and in vitro methods. The four pyrazolone derivatives evaluated (1a, 1b, 1c, and 1d) inhibited over 50% of ZIKV replication with low cytotoxicity. Among them, compound 1b exhibited the most potent activity (EC50 = 4.3 μM) and the highest selectivity (SI = 342). Mechanism of action studies indicated that these compounds act at early stages of virus replication, and compound 1b can also directly inactivate ZIKV particles. Molecular docking studies suggested that these compounds can bind to and block the activity of ZIKV NS5 methyltransferase. Finally, pharmacokinetic and toxicological predictions have reinforced the safety and drug-like profiles of these derivatives. In conclusion, the pyrazolone scaffold proved to be valuable for anti-ZIKV drug development, and the derivatives studied deserve further investigation.

关键词： Zika Pyrazolone NS5 Methyltransferase Antivirals

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Antidiabetic effects of galactomannans from Adenanthera pavonina *** streptozotocin-induced diabetic mice

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Asian Pacific Journal of Tropical Medicine 2018年第2期11卷 116-122页

作者： Icaro Gusmao Pinto Vieira Francisca Noelia Pereira Mendes Sabrina Cesar da silva Raquel Teixeira Terceiro Paim Bruno Bezerra da silva Stephen Rathinaraj Benjamin Eridan Orlando Pereira Tramontina Florean Maria izabel Florindo Guedes Technological Development Park(PADETEC) Federal University of CearaPici Campus60455-970FortalezaCearaBrazil Department of Health and Nutrition State University of CearaAv.Dr.Silas Munguba 1700Itaper campus60714-903ForalezaCearaBrazil

Objective: To evaluate the antidiabetic effect of galactomannans extracted from Adenanthera pavonina's ***(GAP) in streptozotocin(STZ) induced diabetic ***:The preliminary galactomannan yield from Adenanthera pavonina *** and extraction products composition were *** chemical characterization methods like thin layer chromatography, Fourier transform infrared spectroscopy, ~1H and ^(13)C nuclear magnetic resonance, and molecular weight by gel permeation chromatography have been employed to characterize the extracted *** mice were divided in four groups: Normal control, diabetic control, GAP(1% and 2%) treated and standard drug treated *** mice received treatment daily for 30 *** was induced by STZ at a single dose of 120 mg/*** weight, water and food intake, fasting blood glucose, total cholesterol and triglycerides were *** analysis of pancreas and liver were performed to evaluate STZ-induced tissue ***: The isolated and extracted galactomannan from Adenanthera pavonina was confirmed by various chemical characterization *** exhibited a 1.46:1 mannose: galactose ratio, and high molar *** GAP enriched food decreased glycaemia, total cholesterol and *** didn't interfere on food intakes or body weight, although it increased water ***, the relative liver weight indicated toxic galactomannan effects on the histopathological changes of the pancreas in STZ induced ***: It is concluded that GAP is a natural product that contains potent galactomannan and is useful in preventing and treating diabetes.

关键词： Adenanthera pavonina Galactomannans Diabetes Glycaemia Total cholesterol Triacylglycerol

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Antihyperglycemic effect of Passiflora glandulosa cav. fruit rinds flour in streptozotocin-induced diabetic mice

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Asian Pacific Journal of Tropical Medicine 2018年第9期11卷 510-517页

作者： Erlandia Alves Magalhaes Queiroz Raquel Teixeira Terceiro Paim Sandra Machado Lira Jose Ytalo Gomes da silva Carla Laine silva Lima Marcelo Oliveira Holanda Stephen Rathinaraj Benjamin Icaro Gusmao Pinto Vieira Maria izabel Florindo Guedes Biotechnology and Molecular Biology Laboratory State University of CearaFortaleza Ceara Brazil Technological Development Park(PADETEC) Federal University of CearaPici Campus Fortaleza Ceara Brazil

Objective:To investigate the effect of administration of Passiflora glandulosa(***) fruit rinds flour on streptozotocin(STZ)-induced diabetic ***:The preliminary phytochemical screening and parameters such as centesimal composition and brine shrimp toxicity were *** in vivo study Swiss female mice were divided into four groups:NC-normal control;DC-diabetic control animals receiving saline;MET-diabetic animals receiving metformin(200 mg/kg);PFRF-diabetic animals receiving *** fruit rinds flour(200 mg/kg).All of them were treated for 28 *** was used in a single dose of 120 mg/kg to establish diabetic *** weight,water and food intake,fasting blood glucose were *** analysis of pancreas and liver were performed to evaluate STZ-induced tissue ***:Phytochemical screening showed the presence of flavanones and *** *** fruit rinds flour was non-toxic by the brine shrimp *** fruit rinds flour also reduced the loss of body weight and significantly decreased food intake in the diabetic ***,a significant reduction in blood glucose was observed for 15 d and this was maintained on 21 d and 28 d when compared with diabetic ***,the *** fruit rinds flour has a favourable effect on the histopathological changes of the pancreas in STZ induced ***:It is concluded that *** fruit rinds flour is a natural product that contains potent antioxidant compounds and presents good prospects for the improvement of diabetic mellitus by reducing serum glucose levels.

关键词： Diabetes mellitus Passiflora Blood glucose Histology Streptozotocin Mice

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The influence of porosity on tablet subdivision

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Particuology 2020年第6期18卷 192-196页

作者： Maira T.Teixeira Livia L.Sa-Barreto izabel C.silva Tais Gratieri Guilherme M.Gelfuso Ricardo N.Marreto Mariana Landin Marcilio Cunha-Filho Laboratory of Food Drugs and Cosmetics(LTMAC)University of Brasilia(UnB)70910-900 BrasiliaDFBrazil Laboratory of Nanosystems and Drug Delivery Devices(NanoSYS) Federal University of GoiasGoianiaGO.Brazil Departamento de Farmacologia Farmacia y Tecnologia Farmaceutica1+D Farma Group(Gl-1645)Universidade de Santiago de CompostelaSpain

A tablet microstructure,especially the porosity,is a crucial parameter that influences the mechanical ***,tablet subdivisions were studied as a functi on of tablet *** tablets were manufactured in the presenee of different diluents,namely microcrystalline cellulose,LudipressR,or lactose ***,the add让ion of Camphor was investigated,which was thereafter sublimated with a view of obtaining tablets having varying degrees of *** assays were corre lated to the subdivision performs *** increase in porosity reduced the hardness and increased the tablet friability,adversely impacting the *** all the excipients,an increase in relative porosity>90%represented the threshold level from which an inadequate subdivision *** in crease in tablet porosity led to a reduction in mechanical resista nee,which,combined with a heterogeneous and disc on tinuous distribution of pores within the matrix,resulted in poor subdivision *** trolling tablet porosity is an important consideration when designing tablets having a subdivision purpose.

关键词： Tablet subdivision Porosity Mass variation Mass loss Friability Hardness

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Construction and Validation of an Educational Technology for Mammary Health Promotion

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Open Journal of Nursing 2015年第11期5卷 965-970页

作者： izabel Cristina Falcao Juvenal Barbosa Ana Fatima Carvalho Fernandes Camila Brasil Moreira Míria Conceicao Lavinas santos Altamira Mendonca Félix Gomes Ivna silva Andrade Anna Paula Sousa da silva Department of Nursing Federal University of PiauíFlorianoPiauíBrazil

Mammary health is issue faced by public health nurses and the science follows new ways into this thematic area of learning course wares. The purpose of this study was to construct and to validate a distance course for mammary health promotion for nurses. Methodological proposal has been composed of five stages: 1) analysis and planning, 2) modeling, 3) implementation, 4) evaluation and maintenance and 5) distribution. A total of nine experts as research population were consulted who were expert judges of nursing, pedagogy and web designer, three of each specific area, which were included in the study. The variables that had concordance index of 50% were: graphic designer pages favors learning, the user has easy navigation, the learning of the student experience, the environment provides interdisciplinary, content is built on process rather than there is a unique sequence, allows access to the available environment and holds a discussion on the subject. The distance learning course is intended to broaden and to enrich the living spaces and construct their own knowledge, favors interactivity, communication, autonomy and cooperation between the participants and provides access to educational technologies.

关键词： Distance Education Educational Technology Breast Neoplasms Health Promotion Nursing

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Bibliometric Analysis of Nursing Articles Focused on Health Education

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Open Journal of Nursing 2014年第4期4卷 223-230页

作者： Carla Monique Mourao Ana izabel Nicolau Marília Marques Ana Fátima Fernandes Maria Josefina silva Ana Karina Pinheiro Francisca Elisangela Lima Thelma Araújo Camila Moreira Department of Nursing Federal University of Ceará Fortaleza Brazil

This study aimed to analyze the references of articles with the approach to Health Education, published in Brazilian journals of nursing classified as Qualis Nursing A1 and A2. Descriptive study. Setting: SciELO library. We analyzed the references of 113 articles which were used to study the theoretical and methodological framework of bibliometrics, which aimed to promote subsidies for obtaining evaluation indicators of production, contributing to the expansion of scientific knowledge produced and disseminated among authors and researchers in nursing science. The main results showed the prevalence of article such as document most often quoted, references with 5-9 years of temporality, from Brazil and in Portuguese. The journals most frequently used were the Revista Brasileira de Enfermagem e a Revista Latino Americana de Enfermagem. Provided a vision of what is being referenced in nursing publications in Brazil, with regard to the Health Education.

关键词： Nursing Bibliometrics Scientific Health Education

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