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Ornithine aspartate effects on bacterial composition and metabolic pathways in a rat model of steatotic liver disease

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World Journal of Hepatology 2024年第5期16卷 832-842页

作者： Elisa Carolina Lange Pabulo Henrique Rampelotto Larisse Longo Laura Bainy Rodrigues de freitas Carolina Uribe-Cruz Mario reis Alvares-da-Silva Experimental Laboratory of Hepatology and Gastroenterology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-007Brazil Graduate Program in Gastroenterology and Hepatology Universidade Federal do Rio Grande do SulPorto Alegre 90035-007Brazil Bioinformatics and Biostatistics Core Facility Universidade Federal do Rio Grande do SulPorto Alegre 91501-970Brazil Graduate Program in Biological Sciences:Pharmacology and Therapeutics Universidade Federal do Rio Grande do SulPorto Alegre 90035-007Brazil Facultad de Ciencias de la Salud Universidad Católica de las MisionesPosadasMisiones 3300Argentina Division of Gastroenterology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-007RSBrazil Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq)researcher Brasília 71605-001Brazil

BACKGROUND Metabolic-dysfunction associated steatotic liver disease(MASLD)is a hepatic manifestation of metabolic *** suggest ornithine aspartate(LOLA)as drug *** To analyze the influence of LOLA intake on gut microbiota using a nutritional model of *** Adult male Sprague Dawley rats were randomized into three groups:Control(10 rats fed with a standard diet),MASLD(10 rats fed with a high-fat and choline-deficient diet),and LOLA(10 rats receiving 200 mg/kg/d LOLA,after the 16th week receiving high-fat and choline-deficient diet).After 28 wk of the experiment,animals were euthanized,and feces present in the intestine were *** fecal DNA extraction,the V4 region of the 16S rRNA gene was amplified followed by sequencing in an Ion S5™*** Alpha and beta diversity metrics were comparable between MASLD and LOLA.3 OTUs were differentially abundant between MASLD and LOLA,which belong to the species Helicobacter rodentium,Parabacteroides goldsteinii,and Parabacteroides *** functional prediction provided two different metabolic profiles between MASLD and *** 9 pathways differentially abundant in MASLD are related to a change in energy source,adenosine/purine nucleotides degradation as well as guanosine and adenosine deoxyribonucleotides *** 14 pathways differentially abundant in LOLA are associated with four major metabolic functions primarily influenced by L-aspartate,including tricarboxylic acid cycle pathways,purine/guanosine nucleotides biosynthesis,pyrimidine ribonucleotides biosynthesis and salvage as well as lipid IVA *** Although LOLA had no influence on alpha and beta diversity in this nutritional model of MASLD,it was associated with changes in specific gut microbes and their related metabolic pathways.

关键词： Animal model Gut microbiota Metabolic-associated steatotic liver disease Metabolic prediction Ornithine aspartate

Passivation of the Zinc Anode of a Crude Oil Vertical Separator Resulting in Corrosion

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材料科学与工程（中英文B版） 2016年第2期6卷 63-67页

作者： Femando Benedlcto Mamler Juan Manuel Pardal Pedro Paulo Barbosa Leite marcone freitas dos reis Engineering School Federal Fluminense University (UFF) Niteroi Rio de Janeiro 24220-261 Brazil

Interaction between cannabinoid and nucleotide systems as a new mechanism of signaling in retinal cell death

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Neural Regeneration Research 2019年第12期14卷 2093-2094页

作者： Hércules R. freitas Ricardo A. M. reis Ana L. M. Ventura Guilherme R. Franca Laboratory of Neurochemistry Institute of Biophysics Carlos Chagas Filho Universidade Federal do Rio de Janeiro Rio de Janeiro RJ Brazil School of Health Sciences Centro Universitário IBMR Rio de Janeiro RJ Brazil Laboratory of Neurochemistry Institute of Biology Universidade Federal Fluminense Niterói RJ Brazil Biomedical Institute Departament of Physiological Sciences/ Pharmacology Universidade Federal do Estado do Rio de Janeiro Rio de Janeiro RJ Brazil

Nervous system emerges from complex signaling interactions where extrinsic (neurotransmitters and trophic factors, among others) and intrinsic factors (transcription factors) interplay in the developing tissue to control gene activity promoting chronic changes in cell genesis, migration, differentiation and death. The retinal microenvironment is regulated by a broad variety of chemicals, including endocannabinoids and nucleotides that modulate embryonic progenitor-neuron-Müller glia signaling in very early developing or pathophysiological conditions.

关键词： Interaction nucleotide systems cell death

Hepatocellular carcinoma staging systems: Hong Kong liver cancer vs Barcelona clinic liver cancer in a Western population

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World Journal of Hepatology 2019年第9期11卷 678-688页

作者： Laura Bainy Rodrigues de freitas Larisse Longo Deivid Santos Ivana Grivicich Mário reis Alvares-da-Silva Graduate Program in Gastroenterology and Hepatology Universidade Federal do Rio Grande do SulPorto AlegreRS 90035-003Brazil Experimental Hepatology and Gastroenterology Laboratory Center for Experimental ResearchHospital de Clínicas de Porto AlegrePorto AlegreRS 90035-003Brazil School of Medicine Universidade Federal do Rio Grande do SulPorto AlegreRS 90035-003Brazil Graduate Program in Health-Applied Cellular and Molecular Biology ULBRA.CanoasRS 92425-900Brazil Department of Gastroenterology Hospital de Clínicas de Porto AlegrePorto AlegreRS 90035-003Brazil

BACKGROUND Despite being the world’s most widely used system for staging and therapeutic guidance in hepatocellular carcinoma(HCC)treatment,the Barcelona clinic liver cancer(BCLC)system has limitations,especially regarding intermediate-grade(BCLC-B)*** recently proposed Hong Kong liver cancer(HKLC)staging system appears useful but requires validation in Western *** To evaluate the agreement between BCLC and HKLC staging on the management of HCC in a Western population,estimating the overall patient *** This was a retrospective study of HCC patients treated at a university hospital in southern Brazil between 2011 and ***,clinical,and laboratory data were *** staging was carried out according to the HKLC and BCLC systems to assess treatment *** survival was estimated based on the treatment proposed in each *** A total of 519 HCC patients were *** these,178(34.3%)were HKLC-I;95(18.3%)HKLC-IIA;47(9.1%)HKLC-IIB;29(5.6%)HKLC-IIIA;30(5.8%)HKLCIIIB;75(14.4%)HKLC-IV;and 65(12.5%)*** to the BCLC,25(4.9%)were BCLC-0;246(47.4%)BCLC-A;107(20.6%)BCLC-B;76(14.6%)BCLCC;and 65(12.5%)*** general agreement between the two systems was 80.0%-BCLC-0 and HKLC-I(100%);BCLC-A and HKLC-I/HKLC-II(96.7%);BCLC-B and HKLC-III(46.7%);BCLC-C and HKLC-IV(98.7%);BCLC-D and HKLC-V(41.5%).When sub-classifying BCLC-A,HKLC-IIB,HKLC-IIIA and HKLC-IIIB stages according to the up-to-7 in/out criterion,13.4,66.0,100 and 36.7%,respectively,of the cases were classified as up-to-7 *** In a Western population,the general agreement between the two systems was 80.0%,although in BCLC-B cases the agreement was low,suggesting that some individuals could be candidates for the curative treatment recommended by the *** authors suggest that the BCLC system should be routinely employed,although for BCLC-B cases it should be associated with the HKLC system.

关键词： Barcelona clinic liver cancer staging system Hepatocellular carcinoma Hong Kong liver cancer staging system

Evaluation of physicochemical properties as supporting information on quality control of raw materials and veterinary pharmaceutical formulations

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Journal of Pharmaceutical Analysis 2018年第3期8卷 168-175页

作者： Sara da Silva Anacleto Marcella Matos Cordeiro Borges Hanna Leijoto de Oliveira Andressa reis Vicente Eduardo Costa de Figueiredo marcone Augusto Leal de Oliveira Barbara Juliana Pinheiro Borges Marcelo Antonio de Oliveira Warley de Souza Borges Keyller Bastos Borges Departamento de Ciencias Naturals Universidade Federal de Sao Joao del-Rei Campus Dom Bosco Praca Dora Helvecio 74 Fabricas 36301-160 Sao Joao del-Rei Minas Gerais Brazil Departamento de Quimica Universidade Federal do Espirito Santo Avenida Fernando Ferrari 514 Goiabeiras 29075-910 Vitdria Espirito Santo Brazil Laboratorio de Analise de Toxicantes e Farmacos Faculdade de Ciencias Farmaceuticas Universidade Federal de Alfenas Rua Gabriel Monteiro da Silva 700 Centro 37130-000 Alfenas Minas Gerais Brazil Departamento de Quimica Universidade Federal de Juiz de Fora Rua Jose Lourenco Kelmer s/n 36036-330 Juiz de Fora Minas Gerais Brazil Centro de Ciencias da Saude Universidade Federal do Espirito Santo Avenida Marechal Campos 1468 Maruipe 29.043-900 Vitori Espirito Santo Brazil Centro Universitario Norte do Espirito Santo Universidade Federal do Espirito Santo Br 101 Norte Km 67 Sao Mateus Espirito Santo Brazil

This study aimed to show that the physicochemical proprieties obtained by Fourier transform infrared spectroscopy （FTIR）, thermogravimetry （TG）, and scanning electronic microscopy （SEM） can be useful tools for evaluating the quality of active pharmaceutical ingredients （APIs） and pharmaceutical products. In addition, a simple, sensitive, and efficient method employing HPLC-DAD was developed for simulta- neous determination of lidocaine （LID）, ciprofloxacin （CFX） and enrofloxacin （EFX） in raw materials and in veterinary pharmaceutical formulations. Compounds were separated using a Gemini Cm （250 mm×4.6 mm, 5μm） Phenomenex column, at a temperature of 25℃, with a mobile phase containing 10 mM of phosphoric acid （pH 3.29）： acetonitrile （85.7：14.3, v/v） and a flow rate of 1.5 mL/min. Physicochemical characterization by TG, FTIR, and SEM of raw materials of LID, CFX, and EFX provided information useful for the evaluation, differentiation, and qualification of raw materials. Finally, the HPLC method was proved to be useful for evaluation of raw material and finished products, besides satisfying the need for an analytical method that allows simultaneous determination of EFX, CFX, and LID, which can also be extended to other matrices and applications.

关键词： HPLC Physicochemical characterization Quality control Veterinary pharmaceutical formulation Raw material

Chromate and the Environment： Removal and Utilization of Industrial Waste

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Journal of Chemistry and Chemical Engineering 2016年第3期10卷 147-152页

作者： Femando B. Mainier Pedro Paulo B. Leite marcone F. reis Thiago Teobaldo Silva Engineering School Federal Fluminense University(UFF) Niteroi Rio de Janeiro 24220-261 Brazil

Chromate and dichromate sodium as a function of oxidizer characteristics are used in several industrial areas; for example, in surface protection of coated parts of cadmium, zinc and aluminum （chromate coated treated）, corrosion inhibitors, the treatment of leather, the manufacture of pigments, etc. However, the use of such products has been questioned due to the problems of toxicity and pollution that can be caused in the environmental. The Brazilian environmental agency has established that the concentrations of chromate in water courses are less than 0.5 ppm. In order to reuse chromate （CrO42） from industrial effluent, laboratory experiments have been proposed based on chemical reduction or electrolytic processes, in order to transform these chromate ions in a final mix of oxides （in solid form）, which can then be packed and sent to the production process of sodium chromate. The results of these experiments have become useful industrially （without regard to costs） considering the environmental reuse and the life cycle of the chemical compound.

关键词： Chromate dichromate contamination chemical reduction electrolytic process.

Maternal hyperthyroidism increases the synthesis activity and the osteogenic markers expression of calvarial osteoblasts from offspring in a murine model

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BIOCELL 2023年第2期47卷 423-430页

作者： FABIANA ROCHA ARAÚJO BRUNO MACHADO BERTASSOLI ISABELLA CRISTINA SOUZA FÉLIX DOUGLAS MARINHO ABREU NATÁLIA MELO OCARINO AMANDA MARIA SENA reis JUNEO freitas SILVA ROGÉRIA SERAKIDES Núcleo de Células Tronco e Terapia Celular Animal(NCT-TCA) Veterinay SchoolUniversidade Federal de Minas GeraisBelo HorizonteMinas Gerais31270-901Brasil Universidade de Uberaba(UNIUBE) UberabaMinas Gerais38065-300Brasil Instituto de Ciências Biológicas Universidade Federal de Minas GeraisBelo HorizonteMinas Gerais31270-901Brasil Universidade Estadual de Santa Cruz Campus Soane Nazare de AndradeIlheusBahiaBrasil

To evaluate the characteristics and synthesis activity of osteoblasts extracted from the calvaria of offspring of rats exposed to maternal *** adult Wistar rats were divided into two groups,one control and one treated with daily administration of L-thyroxine by an orogastric tube(50μg/animal/day)during *** days after delivery and confirmation of the mothers’hyperthyroidism,the offspring were euthanized for the extraction of osteoblasts from the *** 7,14,and 21 days,proliferation activity was assessed using MTT assay,while alkaline phosphatase(ALP)activity was assessed by the BCIP/NBT *** 21 days,the total area of the mineralized matrix stained by von Kossa was evaluated by *** expression of gene transcripts for Runx2,Bmp2,Fgfr1,collagen type 1(Col1),osteocalcin(Oc),and osteopontin(Op)were evaluated by real-time *** were compared using the Student’s *** activity was significantly higher at 14 and 21 days in cultures of osteoblasts extracted from offspring exposed to maternal hyperthyroidism,while MTT conversion was significantly lower at 21 days in this *** cultures of neonates exposed to maternal hyperthyroidism also showed a larger total area of mineralized matrix and greater expression of gene transcripts for Oc and *** hyperthyroidism increases the activity of matrix synthesis,alkaline phosphatase activity,and expression of gene transcripts for osteocalcin and osteopontin in the osteoblasts,extracted from the calvaria of the offspring,which may be one of the mechanisms of premature fusion of cranial sutures.

关键词： Craniosynostosis Intramembranous growth Ossification Rat Thyroid dysfunction

Design and Transdermal Delivery of Indomethacin Nanosystem

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材料科学与工程（中英文A版） 2011年第4X期1卷 531-537页

作者： Catarina Pinto reis Felipe de freitas Nunes Catarina Rosado LuisMonteiro Rodrigues CBIOS UniversidadeLusofona de Humanidades e Tecnologias Campo Grande 376 Lisboa 1749-024 Portugal Federal University of Parano Av. Pref LothorioMeissner 632 Curitiba 80210-170 Brazil

Transdermal drug delivery is one of the most promising routes of drug administration. The aim of this study was to apply nanoencapsulation technologies in order to enhance bioavailability and reduce toxicity of indomethacin after transdermal administration. Methods: Nanoparticles were produced by a two-step desolvation process. Parameters such as mean particle size, zeta potential and encapsulation efficiency were studied. A model drug was selected for encapsulation-indomethacin. The permeability of free-indomethacin in a cellulose-based vehicle and that of indomethacin-loaded nanoparticles was investigated using Franz diffusion cells. The cumulative amount of indomethacin that diffused across a model membrane was measured and the steady-state fluxes of the permeant in the different systems were determined. Results: This study showed that it is possible to prepare indomethacin-loaded gelatine nanoparticles with small particle size (mean size lower than 350 nm) with polydispersivity index ranging from 0.125 to 0.199 and negative zeta potential. Encapsulation efficiency was around 70%. Permeation studies showed that nanoparticles prolonged indomethacin release. In conclusion, the present data confirm the feasibility of developing indomethacin transdermal nanosystems on an industrial scale. Further studies, now in progress, will deal with the application of the presently reported findings to human skin permeation, involving both in vitro and in vivo testing.

关键词：吲哚美辛透皮给药纳米系统纳米粒子设计 zeta电位 Zeta电位平均粒径

Impact of Drug-Drug Interaction between CDK4/6 Inhibitors and Proton Pump Inhibitors on Survival Outcomes in the Treatment of Metastatic Breast Cancer—Real World Data from a Portuguese Center

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Journal of Cancer Therapy 2022年第5期13卷 266-274页

作者： Joana reis Inês Costa Mariana Costa Ana Valente Catarina Almeida Marta freitas Cláudia Caeiro Catarina Fernandes Nuno Tavares Miguel Barbosa Centro Hospitalar SÃ O JOÃ O E.P.E. Porto Portugal

Introduction: Proton pump inhibitors (PPi) are widely prescribed, including in patients undergoing treatment for advanced breast cancer (ABC). Due to the pharmacokinetic characteristics of the CDK4/6 inhibitor (Ci) palbociclib a drug interaction with PPi was hypothesized. It was shown in a retrospective study that this association was an independent predictive factor for worse progression-free survival (PFS). Objective: To verify the impact of concomitant administration of PPi with Ci on overall survival (OS) and PFS. Material and Methods: This is a retrospective cohort study of patients treated with Ci for HR+HER2-ABC in the period from Feb/2017 to Aug/2020. SPSS software was used for data processing. Univariate analysis was done by the Kaplan-Meier method and log-rank test, and multivariate analysis by COX regression. P-value < 0.05 was considered significant. Results: 80 patients were included. The median age at diagnosis of ABC was 56 years (25 - 75). Treatment with Ci was 1st line for ABC in 68.8%. Choice of Ci was palbociclib in 73.8% (n = 59) and ribociclib in 26.3% (n = 21). The hormone partner was a nonsteroidal aromatase inhibitor in 45.0%, and fulvestrant in 55.0% of cases. 37.5% of patients were on PPi, and 70.0% of them were during the entire treatment (23.3% omeprazole, 73.4% pantoprazole, 3.3% others). Patients taking concomitant PPi and Ci had lower OS (OS-3 years 42.6% vs. 63.4%, p = 0.254) and PFS (PFS med 15 m. vs. 21 m., p = 0.733), although with no statistically significant difference. Discussion: In the sample, there was a numerical difference, without the statistical significance in the use of PPi in the survival of patients under Ci. This difference could be more evident with a longer follow-up and a larger sample size. This study intends to alert to the growing importance of checking for drug interactions. Polymedication, advanced age and the presence of several comorbidities are real problems in patients with ABC. Conclusion: Real-world dat

关键词： Drug Interaction Survival Impact Advanced Breast Cancer CDK4/6 Inhibitors Proton Pump Inhibitors