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Optical clearing mechanisms characterization in muscle

Journal of Innovative Optical Health Sciences 2016年第5期9卷 82-100页

作者： Luís oliveira m.Inês Carvalho Elisabete Nogueira Valery V.Tuchin Physics Department–Polytechnic Institute of Porto School of Engineering Rua Dr.Antonio Bernardino de Almeida 4314200-072 PortoPortugal FEUP–University of Porto Rua Dr.Roberto Frias 4200-465 PortoPortugal CIETI–Centre of Innovation in Engineering and Industrial Technology ISEP Rua Dr.Antonio Bernardino de Almeida4314200-072 PortoPortugal DEEC/FEUP and INESC TEC University of Porto Rua Dr.Roberto Frias 4200-465 PortoPortugal Research-Education Institute of Optics and Biophotonics Saratov National Research State University 83 Astrakhanskaya street Saratov 410012Russia Laboratory of Laser Diagnostics of Technical and Living Systems Institute of Precision mechanics and Control RAS 24 Rabochaya street Saratov 410028Russia Interdisciplinary Laboratory of Biophotonics Tomsk National Research State University 36 Lenin's avenue Tomsk 634050Russia

Optical immersion clearing is a technique that has been widely studied for more than two decades and that is used to originate a temporary transparency effect in biological *** applied in cooperation with clinical methods it provides optimization of diagnosis and treatment *** technique turns biological tissues more transparent through two main mechanisms-tissue dehydration and refractive index(RI)matching between tissue *** matching is obtained by partial replacement of interstit ial water by a biocompat ible agent that presents higher RI and it can be completely rever sible by natural rehydration in vrivo or by assisted rehydration in ex vivo *** data to characterize and discriminate between the two mechanisms and to find new ones are *** a simple method,based on collimated transmittance and thickness measurements made from muscle samples under treatment,we have estimated the diffusion properties of glucose,ethylene glycol(EG)and water that were used to perform such char acterization and *** these properties with data from literature that characterize their diffusion in water we have observed that musde cell membrane permeability limits agent and water diffusion in the *** same experi-mental data has allowed to calculate the optical dearing(OC)eficiency and make an inter-pretation of the internal changes that occurred in muscle during the *** same methodology can now be used to perform similar studies with other agents and in other tissues in order to solve engineering problems at design of inexpensive and robust technologies for a *** improvement of optical tomogr aphic techmiques with better contrast and in depth imaging.

关键词： Collimated transmittance agent diffusivity tissue dehydration thickness variation refractive index matching glucose ethylene glycol

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Extreme liver resections with preservation of segment 4 only

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World Journal of Gastroenterology 2017年第26期23卷 4815-4822页

作者： Silvio marcio Pegoraro Balzan Vinícius Grando Gava marcelo Arbo magalhaes marcelo Luiz Dotto Department of Pharmacy and Biology University of Santa Cruz do Sul Department of Surgery Hospital Moinhos de Vento

To evaluate safety and outcomes of a new technique for extreme hepatic resections with preservation of segment 4 only. mETHODSThe new method of extreme liver resection consists of a two-stage hepatectomy. The first stage involves a right hepatectomy with middle hepatic vein preservation and induction of left lobe congestion; the second stage involves a left lobectomy. Thus, the remnant liver is represented by the segment 4 only (with or without segment 1, ± S1). Five patients underwent the new two-stage hepatectomy (congestion group). Data from volumetric assessment made before the second stage was compared with that of 10 matched patients (comparison group) that underwent a single-stage right hepatectomy with middle hepatic vein preservation. RESULTSThe two stages of the procedure were successfully carried out on all 5 patients. For the congestion group, the overall volume of the left hemiliver had increased 103% (mean increase from 438 mL to 890 mL) at 4 wk after the first stage of the procedure. Hypertrophy of the future liver remnant (i.e., segment 4 ± S1) was higher than that of segments 2 and 3 (144% vs 54%, respectively, P median remnant liver volume-to-body weight ratio was 0.3 (range, 0.28-0.40) before the first stage and 0.8 (range, 0.45-0.97) before the second stage. For the comparison group, the rate of hypertrophy of the left liver after right hepatectomy with middle hepatic vein preservation was 116% ± 34%. Hypertrophy rates of segments 2 and 3 (123% ± 47%) and of segment 4 (108% ± 60%, P > 0.05) were proportional. The mean preoperative volume of segments 2 and 3 was 256 ± 64 cc and increased to 572 ± 257 cc after right hepatectomy. mean preoperative volume of segment 4 increased from 211 ± 75 cc to 439 ± 180 cc after surgery. CONCLUSIONThe proposed method for extreme hepatectomy with preservation of segment 4 only represents a technique that could allow complete resection of multiple bilateral liver metastases.

关键词： Hepatectomy Colorectal liver metastases Hepatic congestion Liver regeneration Liver resection Liver failure

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Object-based image analysis supported by data mining to discriminate large areas of soybean

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International Journal of Digital Earth 2019年第3期12卷 270-292页

作者： Carlos Antonio da Silva Junior marcos Rafael Nanni José Francisco de oliveira-Júnior Everson Cezar Paulo Eduardo Teodoro Rafael Coll Delgado Luciano Shozo Shiratsuchi muhammad Shakir marcelo Luiz Chicati Geotechnology Applied in Agriculture and Forest(GAAF) State University of Mato Grosso(UNEMAT)Alta FlorestaMato GrossoBrazil Department of Agronomy(DAG/PGA) State University of Maringá(UEM)MaringáParanáBrazil Institute of Atmospheric Sciences(ICAT) Federal University of Alagoas(UFAL)MaceióAlagoasBrazil Federal University of mato Grosso do Sul(UFmS) Chapadão do SulMato Grosso do SulBrazil Department of Environmental Sciences(DCA) Forestry Institute(IF)Federal Rural University of Rio de Janeiro(UFRRJ)SeropédicaRio de JaneiroBrazil AgCenter Louisiana State University(LSU)Baton RougeLAUSA Institute of Space Technology Islamabad Pakistan IslamabadPakistan

This research aimed to analyze the possibility to estimate and automatically map large areas of soybean cultivation through the use of mODIS(moderate-Resolution Imaging Spectroradiometer)*** major techniques were used:GEOgraphic-Object-Based Image Analysis(GEOBIA)and Data mining(Dm).In order to obtain the images,the segmentation algorithm implemented by Definiens Developer was used.A decision tree(DT)was created from a training set previously ***-series of images from the mODIS sensor aboard the Terra satellite were acquired in order to represent the wide variation of the vegetation pattern along the soybean crop *** time-series data were used only for the CEI ***,to compare the results obtained from GEOBIA,the slicing technique was used at the CEI *** the training,the DT was applied to the vegetation indices generating the thematic map of the spatial distribution of *** accordance with the error matrix and kappa parameter analysis,tests for statistical significance were *** indicate that the classification achieved by Kappa coefficients is *** short,the obtained results proved that combining vegetation indices and time-series data using GEOBIA return promising results for mapping soybean plantation on a regional scale.

关键词： GEOBIA time-series vegetation indices mODIS vegetation dynamics

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Decontamination of mobile phones and electronic devices for health care professionals using a chlorhexidine/carbomer 940■gel

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Frontiers of Chemical Science and Engineering 2019年第1期13卷 192-198页

作者： Rafael muniz de oliveira Nereida mello da Rosa Gioppo Jancineide oliveirade Carvalho Francilio Carvalho oliveira Thomas Jay Webster Fernanda Roberta marciano Anderson oliveira Lobo University Hospital of the West of Parana State University of West of Parana Cascavel PR Brazil University Center for Health. Humanities and Technology of Piaui (UNINOVAFAPI). Piaui Brazil Science and Technology Institute Brasil University Itaquera SP Brazil Nanomedicine Laboratory Department of Chemical Engineering Northeastern University MA 02115 USA materials Science and Engineering graduation program Technology Center Federal University of Piaui. PI Brazil

Though they reduce microorganism growth, current hospital disinfectants also damage many of today’s modern electronic devices such as tablets and smartphones. Herein, the efficacy of a new chlorhexidine digluconate gel (CDG) was tested as a disinfectant for mobile and electronic devices in a clinical environment. Specifically, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and methicillin resistant Staphylococcus aureus were used to infect the screen of eight smartphones. The CDG was prepared at concentrations of 2%, 4% and 6%, and tested on paper disks infected with these bacteria before being tested on the smartphones. The devices were disinfected with the CDG gel (4%) at two times: immediately and after 5 min of the bacterial contamination. In all cases, the CDG gel eliminated 100% of gram-positive and gram-negative microorganisms compared to the control (without any agent). In addition, the gel did not damage the smartphones. Therefore, our study suggests that the CDG gel may be applied to disinfect a wide range of electronic devices for health care professionals in the hospital environment.

关键词： smartphones disinfection clinical environment chlorhexidine bacteria

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YbF_3/SiO_2 Fillers as Radiopacifiers in a Dental Adhesive Resin

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Nano-micro Letters 2012年第3期4卷 189-196页

作者： Neftali L.V.Carreno Thiago C.S.oliveira Evandro Piva Fernanda B.Leal Giana S.Lima marcelo D.moncks Cristiane W.Raubach Fabricio A.Ogliari Technology Development Center Engineering of MaterialFederal University of Pelotas Biomaterials Development and Control Center School of DentistryFederal University of Pelotas Department of Analytical and Inorganic Chemistry IQGFederal University of Pelotas

The objective of this study was to evaluate the effect of functionalizing a dental adhesive resin with YbF_3/SiO_2 fillers for use as *** of YbF_3/SiO_2 were obtained with the high-energy mechanical milling method and characterized by both physical and chemical *** characterization,the particles were sieved and silanized prior to being incorporated into an adhesive *** stability of the particle suspension was then *** light activation,the radiopacity,degree of conversion,flexural strength and elastic modulus were *** dental adhesive resins with 10 and 15 wt% of filler provided satisfactory radiopacity,while flexural strength and elastic modulus were not *** degree of conversion was statistically lower than that of the control(pmethod used for incorporating the tested ytterbium fluoride/silicon dioxide particles at concentrations of 10 and 15 wt% was shown to be feasible for the development of a radiopaque dental adhesive system.

关键词： Radiopacifier YbF3/SiO2 Dental adhesive resin Composite

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Curcumin-Loaded Electrospun Fibers: Fluorescence and Antibacterial Activity

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Advanced Fiber materials 2020年第5期2卷 256-264页

作者： mário César Albuquerque de oliveira Fernando Antonio Gomes da Silva Jr. mateus matiuzzi da Costa Nikifor Rakov Helinando Pequeno de oliveira Institute of materials Science Federal University of Sao Francisco ValleyJuazeiroBA 48920-310Brazil

The health benefits and sensing applications of curcumin-based systems depend on their prevailing photophysical and chemi-cal properties at a specific environment-the characteristic low solubility of curcumin in water hinders high-dosage based *** circumvent this drawback,curcumin has been incorporated in hydrophobic environments in ***,electrospun fibers of Eudragit L100 were loaded with curcumin and applied in fluorescence and antibacterial assays to evaluate prevailing physical and chemical properties in *** characteristic high loading degree of curcumin(250 mg/mL in Eudragit L100 solution)preserves not only the photophysical properties of molecules(such as the intrinsic fluorescence)but also the antibacterial activity of released molecules from electrospun fibers,characterizing an important strategy for improvement in the applicability potential of natural materials.

关键词： Curcumin Fluorescence Electrospinning Hydrophobic core

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Adenoma incidence decreases u nder the efect of polypectomy

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World Journal of Gastroenterology 2012年第11期18卷 1243-1248页

作者： Isadora Rosa Paulo Fidalgo Jose Soares Susana Vinga Carla oliveira Joao P Silva Susana m Ferro Paula Chaves Antonio G oliveira Carlos N Leitao Gastroenterology Department Instituto Portu-gues de Oncologia de Lisboa Francisco Gentil EPE 1099-023Lisboa Portugal Faculdade de Ciencias da Safldeda Universidade da Beira Interior 6200-506 Covilha Portugal Jose Soares Gastroenterology Department Hospital de SantoAntonio EPE 4099-001 Porto Portugal Instituto de Engenharia de Sistemas e Com-putadores: Investigacao e Desenvolvimento 1000-029 LisboaPortugal Hospital de Santo Antonio EPE 4099-001Porto Portugal Pathology Department Instituto Portugues deOncologia de Lisboa Francisco Gentil EPE 1099-023 LisboaPortugal Biostatistics Department Faculdade deCiencias M6dicas 1169-056 Lisboa Portugal

mm： To investigate whether, under the influence of pol ypectomy, the incidence of adenoma decreases with age.mETHODS： Consecutive patients with colonic adenomas identified at index colonoscopy were retrospectively selected if they had undergone three or more complete colonoscopies, at least 24 mo apart. Patients who had any first-degree relative with colorectal cancer were excluded. Data regarding number of adenomas at each colonoscopy, their location, size and histological classification were recorded. The monthly incidence density of adenomas after the index examination was estimated for the study population, by using the person-years method. Baseline adenomas were excluded from incidence calculations but their characteristics were correlated with recurrence at follow-up, using the X^42 test.RESULTS： One hundred and fifty-six patients were included （109 male, mean age at index colonoscopy 56.8 ± 10.3 years）, with follow-up that ranged from 48 to 232 mo. No significant correlations were observed between the number, the presence of villous component, or the size of adenomas at index colonoscopy and the presence of adenomas at subsequent colonoscopies （P = 0.49, 0.12 and 0.78, respectively）. The incidence of colonic adenomas was observed to decay from 1.4% person-months at the beginning of the study to values close to 0%, at 12 years after index colonoscopy.CONCLUSION： Our results suggest the sporadic formation of adenomas occurs within a discrete period and that, when these adenomas are removed, all neoplasia-prone clones may be extinguished.

关键词： Colorectal cancer Colorectal adenoma In-cidence Age Polypectomy

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Degradation of duloxetine: Identification of transformation products by UHPLC-ESI(+)-HRmS/mS,in silico toxicity and wastewater analysis

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Journal of Environmental Sciences 2019年第8期31卷 113-123页

作者： Rodrigo A.Osawa Ana P.Carvalho Olinda C.monteiro m.Conceicao oliveira m.Helena Florêncio Centro de Química e Bioquímica Faculdade de Ciências Universidade de Lisboa Laboratório de FTICR e Espectrometria de massa Estrutural Faculdade de Ciências Universidade de Lisboa Centro de Química Estrutural Instituto Superior Técnico Universidade de Lisboa

Duloxetine(DUL), an antidepressant drug, has been detected in surface water and wastewater effluents, however, there is little information on the formation of its transformation products(TPs). In this work, hydrolysis, photodegradation(UV irradiation) and chlorination experiments were performed on spiked distillated water, under controlled experimental conditions to simulate abiotic processes that can occur in the environment and wastewater treatment plants(WWTPs). Eleven TPs, nine from reaction with UV light and two from chlorine contact, were formed and detected by ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry, and nine of them had their chemical structures elucidated upon analyses of their fragmentation patterns in mS/mS spectra. The formation and degradation of the TPs were observed. The parent compound was completely degraded after30 min in photodegradation and after 24 hr in chlorination. Almost all TPs were completely degraded in the experiments. The ecotoxicity and mutagenicity of the TPs were predicted based on several in silico models and it was found that a few of these products presented more ecotoxicity than DUL itself and six TPs showed positive mutagenicity. Finally, wastewater samples were analyzed and DUL and one TP, possibly formed by chlorination process, were detected in the effluent, which showed that WWTP not only did not remove DUL, but also formed a TP.

关键词： High resolution mass spectrometry Duloxetine Degradation processes Transformation products Wastewater analysis In silico toxicity

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Recovery of valuable metals from waste cables by employing mechanical processing followed by spouted bed elutriation

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Particuology 2019年第4期17卷 74-80页

作者： Eduardo H. Tanabe Roger m. Silva Daliomar L. oliveira Junior Daniel A. Bertuol Environmental Processes Laboratory (LAPAm) Chemical Engineering Department Federal University of Santa Maria - UFSM Santa Maria RS Brazil

Cables and wires, which are essential parts of equipment for the transmission of both information and electricity, contain valuable materials including copper (Cu) and aluminum (Al). This experimental work evaluates the use of mechanical processing (grinding and sieving), followed by spouted bed elutriation, for separation of the valuable materials (steel-coated copper, copper, and aluminum) present in waste coaxial and internet cables. A recovery efficiency of approximately 90% was achieved for the steel-coated copper in coaxial cables. For in ternet cables, the copper recovery efficiency was approximately 87%. These findings dem on strate that it is possible to separate, recover, and recycle the valuable materials (metals) present in waste cables using simple, low-cost, and efficient mechanical processing followed by spouted bed elutriation.

关键词： Waste cable Elutriation Copper Recycling mechanical process

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A Proposal for Reservoir Volume Calculation in Rainwater Harvesting Systems

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Journal of Civil Engineering and Architecture 2012年第6期6卷 707-714页

作者： Rodrigo Braga moruzzi marcelo Loureiro Garcia Samuel Conceicao de oliveira Geoprocessing and Territorial Planning Department (DEPLAN) Institute of Geosciences and Exact Sciences (IGCE) StateUniversity of Sao Paulo (UNESP) Rio Claro SP 13506-900 Brazil Petrology and metallogeny Department (DPm) Institute of Geosciences and Exact Sciences (IGCE) State University of SaoPaulo (UNESP) Rio Claro SP 13506-900 Brazil

This paper introduces a proposal for reservoir volume calculation in rainwater harvesting systems. The proposed method can be used for reservoir volume design in rainwater harvesting systems and is based on three important variables. These variables are water demand, system efficiency and repayment time. Several simulations were carried out in different scenarios considering typical values of both catchment area （for low-income and medium-income households） and water demand, with fixed water and tank costs. Results showed that the integrated analysis of demand, efficiency and repayment time may assist designers to determine a more adequate reservoir volume.

关键词： Rainwater use reservoir volume design criteria.

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