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Finding my way: The role of dirigent proteins in lignin assembly

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molecular Plant 2024年第2期17卷 230-232页

作者： Dyoni m.oliveira Igor Cesarino Department of Plant Biotechnology and Bioinformatics Ghent University9052 GhentBelgium VIB Center for Plant Systems Biology 9052 GhentBelgium Departamento de Botanica Instituto de BiocienciasUniversidade de Sao PauloRua do Matao277Sao Paulo 05508-090Brazil Synthetic and Systems Biology Center InovaUSPAvenida Professor Lucio Martins Rodrigues370SaoPaulo 05508-020Brazil

Lignin emerged around 450 million years ago in early tracheophytes and played a pivotal role in the colonization of terrestrial ecosystems by *** aromatic biopolymer provides mechanical strength to supportive tissues and hydrophobicity to water-transporting vasculature and acts as a physical barrier against herbivores and pathogens(Renault et al.,2019).The deposition of lignin in cell walls for proper cellular functionalization involves several molecular steps,including the tight regulation of tissue-or cell-type-specific expression of lignin biosynthetic genes,the biosynthesis of distinct lignin monomers and their transportation to the apoplast,and the polymerization of monomer radicals to produce tissue-or cell-type-dependent polymers with distinct physico-chemical properties.

关键词： properties. lignin walls

In the lack of extreme pioneers:trait relationships and ecological strategies of 66 subtropical tree species

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Journal of Plant Ecology 2015年第4期8卷 359-367页

作者： Cristiane Forgiarini Alexandre F.Souza Solon J.Longhi Juliano m.oliveira Programa de Pós-Graduação em Biologia Diversidade e Manejo da Vida SilvestreUniversidade do Vale do Rio dos SinosAv.UNISINOS 950C.P.275São Leopoldo 93022-000RSBrazil Departamento de Ecologia CBUniversidade Federal do Rio Grande do NorteCampus UniversitárioLagoa NovaNatal 59072-970RNBrazil PPG-Engenharia Florestal Dep.de Ciências FlorestaisUniversidade Federal de Santa MariaAv.Roraimas/nBairro CamobiSanta Maria 97105-900RSBrazil Present address:Departamento de Botânica Instituto BiociênciasUniversidade Federal do Rio Grande do SulAv.Bento Gonçalves9500Prédio 43433Campus AgronomiaPorto Alegre 91501-970RSBrazil.

Aims Despite the growing interest in the topic of functional ecology,there are still forest regions that have not been examined,as most work has been done in the *** issues include the strength of a growth-mortality trade-off in trees(originally identified for seedlings)and the nature of the association between plant traits and vital rates,if *** objectives were to examine whether(i)ecological strategies in south american mixed forests are organ-ized along the fast competitor×slow stress-tolerator and height gradients as the main strategy axes depicted in the overall trait and vital rate correlation structure,and(ii)a tentative path model we proposed can explain the patterns of covariation among traits and vital *** We studied a different habitat(subtropical mixed conifer-hardwood forests)and region(brazilian atlantic Forest)from the majority of related studies in forests,carried out in the Neotropical *** on total height,stem slenderness,crown depth,wood density,specific leaf area,leaf and seed length,seed dispersal mode,annual mortality,diameter relative growth rate and relative growth rate under favorable conditions were measured in southern brazil for 66 tree *** were subjected to principal components analysis and path *** data on saplings and treelets were analyzed through *** Findingsstudied traits were reduced to four principal *** components analysis produced axes that fit the resource acquisition versus resource-conservation and the height-mortality trade-offs,although the former was split into two distinct *** size and seed dispersal mode appeared independently of these axes.a path model showed that leaf length and specific leaf area caused direct changes in trunk slenderness and,indirectly through growth,affected *** trade-offs between growth and survivorship and between wood density and stem slenderness trade-offs were not *** may

关键词： Araucaria angustifolia atlantic forest ecological strategies life-history trade-offs mixed forests

ANP impairs the dose-dependent stimulatory effect of ANG II or AVP on H^(+)-ATPase subcellular vesicle trafficking

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Open Journal of molecular and Integrative Physiology 2013年第3期3卷 95-103页

作者： m.oliveira-Souza department of physiology and biophysics instituto de ciencias biomedicasuniversity of sao paulosao paulobrazil

The effect of angiotensin II (ANG II) or arginine vasopressin (AVP) alone or plus atrial natriuretic peptide (ANP) on H+-ATPase subcellular vesicle trafficking was investigated in mDCK cells following intracellular pH (pHi) acidification by exposure to20 mmNH4Cl for 2 min in a Na+-free solution containing Schering 28080, conditions under which H+-AT-Pase is the only cell mechanism for pHi recovery. Using the acridine orange fluorescent probe (5mm) and confocal microscopy, the vesicle movement was quantified by determining, for each experimental group, the mean slope of the line indicating the changes in apical/basolateral fluorescence density ratio over time during the first 5.30 min of the pHi recovery period. Under the control conditions, the mean slope was 0.079 ± 0.0033 min-1 (14) and it increased significantly with ANG II [10-12 and 10-7 m, respectively to 0.322 ± 0.038 min-1 (13) and 0.578 ± 0.061 min-1 (12)] or AVP [10-12 and 10-6 m, respectively to 0.301 ± 0.018 min-1 (12) and 0.687 ± 0.049 min-1 (11)]. However, in presence of ANP (10-6 m, decreases cytosolic free calcium), dimethyl-BAPTA/Am (5 × 10-5 m, chelates intracellular calcium) or colchicine (10-5 m, 2-h preincubation;inhibits microtubule-dependent vesicular trafficking) alone or plus ANG II or AVP the mean slopes were similar to the control values, indicating that such agents blocked the stimulatory effect of ANG II or AVP on vesicle trafficking. The results suggest that the pathway responsible for the increase in cytosolic free calcium and the microtu-bule-dependent vesicular trafficking are involved in this hormonal stimulating effect. Whether cytosolic free calcium reduction represents an important direct mechanism for ANP impairs the dose-dependent stimulatory effect of ANG II or AVP on H+-ATPase subcellular vesicle trafficking, or is a side effect of other signaling pathways which will require additional studies.

关键词： H^(+)-ATPase Vesicle Trafficking ANP ANG II AVP

Indirect printing of hierarchical patient-specific scaffolds for meniscus tissue engineering

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Bio-Design and manufacturing 2019年第4期2卷 225-241页

作者： Joao BCosta Joana Silva-Correia Sandra Pina Alain da Silva morais Sílvia Vieira Hélder Pereira Joao Espregueira-mendes Rui LReis Joaquim m.oliveira B′s Research Group I3Bs-Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAveParkZona Industrial da Gandra4805-017 Barco GmrPortugal ICVS/3B’s-PT Government Associate Laboratory Braga/GuimaraesPortugal The Discoveries Centre for Regenerative and Precision medicine Headquarters at University of MinhoAvepark4805-017 BarcoGuimar'sPortugal Ripoll y De Prado Sports Clinic:murcia-madrid FIFA medical Centre of Excellence MurciaSpain Orthopedic Department Centro Hospitalar Póvoa de Varzim Vila do CondePortugal Clínica do Dragao Espregueira-Mendes Sports CentreFIFA Medical Centre of ExcellencePortoPortugal Dom Henrique Research Centre PortoPortugal Orthopedic Department University of MinhoBragaPortugal

The complex meniscus tissue plays a critical role in the knee. The high susceptibility to injury has led to an intense pursuit for better tissue engineering regenerative strategies, where scaffolds play a major role. In this study, indirect printed hierarchical multilayered sca ffolds composed by a silk fibroin (SF) upper layer and an 80/20 (w/w) ratio of SF/ionic-doped β-tricalcium phosphate (TCP) bottom layer were developed. Furthermore, a comparative analysis between two types of sca ffolds pro- duced using di fferent SF concentrations, i.e., 8% (w/v) (Hi8) and 16% (w/v) (Hi16) was performed. In terms of architecture and morphology, the produced sca ffolds presented homogeneous porosity in both layers and no di fferences were observed when comparing both sca ffolds. A decrease in terms of mechanical performance of the sca ffolds was observed when SF concentration decreased from 16 to 8% (w/v). Hi16 revealed a static compressive modulus of 0.66 ± 0.05 mPa and dynamical mechanical properties ranging from 2.17 ± 0.25 to 3.19 ± 0.38 mPa. By its turn, Hi8 presented a compressive modulus of 0.27 ± 0.08 mPa and dynamical mechanical properties ranging from 1.03 ± 0.08 mPa to 1.56 ± 0.13 mPa. In vitro bioactivity studies showed formation of apatite crystals onto the surface of Hi8 and Hi16 bottom layers. Human meniscus cells (hmCs) and human primary osteoblasts were cultured separately onto the top layer (SF8 and SF16) and bottom layer (SF8/TCP and SF16/TCP) of the hierarchical sca ffolds Hi8 and Hi16, respectively. Both cell types showed good adhesion and proliferation as denoted by the live/dead staining, Alamar Blue assay and DNA quanti fication analysis. Subcutaneous implantation in mice revealed weak in flammation and sca ffold’s integrity. The hierarchical indirect printed SF sca ffolds can be promising candidate for meniscus TE sca ffolding applications due their suitable mechanical properties, good biological performance and possibility of being applied in a patie

关键词： Patient-specific Indirect printing Hierarchical Silk fibroin Enzymatic-cross-linking meniscus

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Distribution of trace metals and Pb isotopes in bottom sediments of the murucupi River,North Brazil

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International Journal of Sediment Research 2016年第3期31卷 226-236页

作者： Diomar Cavalcante oliveira Jean michel Lafon marcelo de oliveira Lima Institute of Geosciences Federal University of Pára Environmental Section Evandro Chagas Institute

The murucupi River belongs to the hydrographic network of the Para River estuary,at the southern portion of the Amazon River mouth,which consists of a fluvial-marine transitional zone under strong impact of both tidal and fluvial *** geochemical results obtained for bottom sediments from the murucupi River,the Arrozal Channel,and the Para River indicate a natural variation of Pb,Cr,Cu,Zn,and Ni content among these water ways with no significant anthropogenic *** to the threshold effects level(TEL),the contents of trace metals do not offer risk to the local *** contrast,the differences in the Pb isotopic composition of sediments in the murucupi River,the Arrozal Channel,and the Para River are *** isotopic signatures indicate an anthropogenic contribution of Pb in the murucupi River originating from the domestic effluents of urban centers;industrial waste represented by red mud is not *** results demonstrate that the Pb isotopic signature is a prospective indicator for future contamination of bottom sediments by trace metals and is useful for identifying contaminants among the possible anthropogenic sources.

关键词： Bottom sediments Pb isotopes Trace metals murucupi River Barcarena

Neurogenesis dynamics in the olfactory bulb:deciphering circuitry organization, function, and adaptive plasticity

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Neural Regeneration Research 2025年第6期20卷 1565-1581页

作者： moawiah m.Naffaa Department of Psychology and Neuroscience Duke UniversityDurhamNCUSA Department of Cell Biology Duke University School of MedicineDurhamNCUSA

Adult neurogenesis persists after birth in the subventricular zone, with new neurons migrating to the granule cell layer and glomerular layers of the olfactory bulb, where they integrate into existing circuitry as inhibitory interneurons. The generation of these new neurons in the olfactory bulb supports both structural and functional plasticity, aiding in circuit remodeling triggered by memory and learning processes. However, the presence of these neurons, coupled with the cellular diversity within the olfactory bulb, presents an ongoing challenge in understanding its network organization and function. moreover,the continuous integration of new neurons in the olfactory bulb plays a pivotal role in regulating olfactory information processing. This adaptive process responds to changes in epithelial composition and contributes to the formation of olfactory memories by modulating cellular connectivity within the olfactory bulb and interacting intricately with higher-order brain regions. The role of adult neurogenesis in olfactory bulb functions remains a topic of debate. Nevertheless, the functionality of the olfactory bulb is intricately linked to the organization of granule cells around mitral and tufted cells. This organizational pattern significantly impacts output, network behavior, and synaptic plasticity, which are crucial for olfactory perception and memory. Additionally, this organization is further shaped by axon terminals originating from cortical and subcortical regions. Despite the crucial role of olfactory bulb in brain functions and behaviors related to olfaction, these complex and highly interconnected processes have not been comprehensively studied as a whole. Therefore, this manuscript aims to discuss our current understanding and explore how neural plasticity and olfactory neurogenesis contribute to enhancing the adaptability of the olfactory system. These mechanisms are thought to support olfactory learning and memory, potentially through increased

关键词： network adaptability neurogenesis neuronal communication olfactory bulb olfactory learning olfactory memory synaptic plasticity

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Fish culture in biofloc technology(BFT):Insights on stocking density carbon sources,C/N ratio,fish nutrition and health

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Aquaculture and Fisheries 2024年第4期9卷 522-533页

作者： Luciana Kelly oliveira Wilson Wasielesky Jr marcelo Borges Tesser Laboratorio de Nutriçao de Organismos Aqu´aticos Instituto de OceanografiaUniversidade Federal do Rio Grande-FURG-Rua do Hotel02-CassinoRio Grande96201-900Brazil Laborat´orio de Carcinocultura Instituto de OceanografiaUniversidade Federal do Rio Grande-FURG-Rua do Hotel02-CassinoRio Grande96201-900Brazil

The growth of aquaculture demands intense consumption of formulated foods,scarce natural resources such as water and *** increase in aquaculture production needs to be sustainable in several aspects of the activity,including the use of more sustainable farming systems that provide reduced water demand,less space dependence for increased production,and availability of complementary natural *** this perspective,the biofloc system(BFT),presents itself as a more friendly cultivation technology as a way to mitigate some impacts of aquaculture *** research has been carried out,addressing different aspects important to the production of fish in *** this review,we address how stocking density,carbon sources and carbon nitrogen ration(C/N)affect animal performance and the nutritional value of bioflocs;the use of microbial aggregates as“in situ”and“ex situ”food and the benefits in feed conversion,and their influence on the immune system and disease resistance.

关键词： Biofloc technology Aquaculture system Natural food Nutritional value Fish health

SBL-JP-0004:A promising dual inhibitor of JAK2 and PI3KCD against gastric cancer

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Oncology Research 2025年第1期33卷 235-243页

作者： HASSAN m.OTIFI Department of Pathology College of MedicineKing Khalid UniversityAbha62521Saudi Arabia

Background:Gastric cancer(GC)remains a global health burden and is often characterized by heterogeneous molecular profiles and resistance to conventional *** phosphoinositide 3-kinase and PI3K and Janus kinase(JAK)signal transducer and activator of transcription(JAK-STAT)pathways play pivotal roles in GC progression,making them attractive targets for therapeutic ***:This study applied a computational and molecular dynamics simulation approach to identify and characterize SBL-JP-0004 as a potential dual inhibitor of JAK2 and PI3KCD *** and SNU-5 GC cells were used for in vitro ***:SBL-JP-0004 exhibited a robust binding affinity for JAK2 and PI3KCD kinases,as evidenced by molecular docking scores and molecular dynamics *** interactions and Gibbs binding free energy estimates confirmed stable and favorable interactions with target ***-JP-0004 displayed an half-maximal inhibitory concentration(IC_(50))value of 118.9 nm against JAK2 kinase and 200.9 nm against PI3KCD ***-JP-0004 exhibited potent inhibition of cell proliferation in KATOIII and SNU-5 cells,with half-maximal growth inhibitory concentration(GI50)values of 250.8 and 516.3 nm,respectively.A significant elevation in the early phase apoptosis(28.53%in KATOIII cells and 26.85%in SNU-5 cells)and late phase apoptosis(17.37%in KATOIII cells and 10.05%in SNU-5 cells)were observed with SBL-JP-0004 treatment compared to 2.1%and 2.83%in their respective ***:The results highlight SBL-JP-0004 as a promising dual inhibitor targeting JAK2 and PI3KCD kinases for treating GC and warrant further preclinical and clinical investigations to validate its utility in clinical settings.

关键词： Gastric cancer(GC) Dual inhibition JAK2 PI3KCD SBL-JP-0004 molecular docking molecular dynamics simulation

mitochondria–cGAS–STING axis is a potential therapeutic target for senescence-dependent inflammaging-associated neurodegeneration

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Neural Regeneration Research 2025年第3期20卷 805-807页

作者： Josém.Izquierdo Centro de Biología molecular Severo Ochoa(CBm) Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid(CSIC-UAM)MadridSpain

The word “senescence” comes from the Latin senescens, meaning “to begin to age”, and is characterized by a long-lasting but reversible block in proliferation, resulting from stress-induced cell cycle arrest of pre... 详细信息

关键词： Latin potential lasting

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