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Transcriptome analysis reveals immune-related genes in tissues of Vibrio anguillarum-infected turbot Scophthalmus maximus

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Journal of Oceanology and Limnology 2024年第1期42卷 332-344页

作者： Yuting SONG maqsood ahmed soomro Xianzhi DONG Guobin HU College of Marine Life Sciences Ocean University of ChinaQingdao 266003China Institute of Evolution&Marine Biodiversity Ocean University of ChinaQingdao 266003China Institute of Biophysis Chinese Academy of SciencesBeijing 100101China

Turbot Scophthalmus maximus is an important mariculture fish species with high economic ***,the bacterial diseases caused by Vibrio anguillarum infection bring huge economic losses to the turbot aquaculture *** understand the immune response of the turbot against *** infection and to explore novel immune-related genes,the transcriptome analysis of turbot spleen and gills were conducted after *** *** expressed genes(DEGs)were identified in spleen and gill of the turbot amounted to 17261 and 16436,respectively.A large number of immunerelated DEGs were enriched in cytokine-cytokine receptor interaction signaling pathway,and the others by the kyoto encyclopedia of genes and genomes(KEGG)*** gene ontology(GO)classification analysis revealed that *** infection had the greatest effect on biological processes and cellular *** immune-related DEGs were identified in the spleen(cstl.1,egfl6,lamb21,v2rx4,calcr,and gpr78a)and gills(ghra,sh3gl2a,cst12,inhbaa,cxcl8,and il-1b)by heat *** proteinprotein interaction(PPI)networks were constructed to analyze the immune *** results demonstrate that the maturation and antigen processing of major histocompatibility complex(MHC)class II molecule,and calcitonin-or adrenomedullin-regulated physiological activity were important events in the immunity of turbot against *** *** the gills,the protein interactions in TGF-βsignaling pathway,production of inflammatory factors,and endocytosis regulation were most *** research laid a foundation for discovering novel immune-related genes and enriching the knowledge of immune mechanisms of turbot against *** infection.

关键词： Scophthalmus maximus Vibrio anguillarum transcriptome differentially expressed genes immune mechanism

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Visual detection of tropomyosin,a major shrimp allergenic protein using gold nanoparticles(AuNPs)-assisted colorimetric aptasensor

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Marine Life Science & Technology 2021年第3期3卷 382-394页

作者： Tushar Ramesh Pavase Hong Lin maqsood ahmed soomro Hongwei Zheng Xiaxia Li Kexin Wang Zhenxing Li Food Safety Laboratory Department of Food Science and EngineeringCollege of Food Science and EngineeringOcean University of ChinaQingdao266003China Fish Molecular Immunology Laboratory College of Marine Life SciencesOcean University of ChinaQingdao266003China

A gold nanoparticle-based label-free colorimetric assay was developed to detect the shrimp allergenic protein tropomyosin(TM),an important biomarker responsible for severe clinical reactivity to *** a gold nanoparticles(AuNPs)-tropomyosin-binding aptamer(TMBA)complex,the aptamer adsorbs onto the surface of AuNPs and dissociates in the presence of *** addition,AuNPs tend to aggregate in the presence of ionic salt,revealing a color change(i.e.,wine-red to purple/blue)with a shift in the maximum absorption peak from 520 *** the presence of specific binding TM,the aptamer folds into a tertiary structure where it more efficiently stabilizes AuNPs toward the salt-induced aggregation with a hypsochromic shift in the absorption spectra compared to the stabilized AuNPs by aptamer *** on the aggregation and sensitive spectral transformation principle,the AuNPs-based colorimetric aptasensor was successfully applied to detect TM with a range of 10-200 nmol/L and a low detection limit of 40 nmol/L in water *** reliability,selectivity,and sensitivity of the aptasensor was then tested with food samples spiked with *** observed detection limit was as low as 70 nmol/L in shrimp,90 nmol/L in tofu,and 80 nmol/L in eggs,*** anticipate the proposed AuNPs-based colorimetric aptasensor assay possesses a high potential for the easy and efficient visual colorimetric detection of TM.

关键词： Gold nanoparticles(AuNPs) Aptamer Aggregation Colorimetric assay Shellfish allergenic protein Shrimp tropomyosin(TM)

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The surveillance of arsenic levels in the drinking water of primary schools and the assessment of the potential cancer-related health risks of children in Multan, Pakistan

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Emerging Contaminants 2024年第1期10卷 17-23页

作者： Hidayatullah Mahar Abdul Rehman Memon Aqsa Ishfaq Suhail ahmed soomro Mehran University of Engineering&Technology JamshoroPakistan NFC Institute of Engineering&Technology MultanPakistan

To investigate the levels of arsenic(As)in the groundwater and drinking water of primary schools and their potential impact on human health is main objective of this *** assess risk,the following parameters i.e.,carcinogenic indices(CI),hazardous indexes(HI),carcinogenic risk(CR),and hazardous quotient(HQ)have been measured for both dermal and oral arsenic *** study has been focused on primary schools located in four tehsils of Multan,where water samples were *** levels,with concentrations ranging from 3.31 to 191 mg/l,exceeding the safe limit set by the World Health Organization(WHO)have been detected in 99.9%of the *** results show that arsenic content ranged from 3.31 to 191 mg/l,with 99.9%of the samples exceeding the World Health Organization's safe limit of 10 mg/*** CR and CI exceeded the USEPA limit of 10–6 in all four tehsils ranged from 2.6×10^(-6) to 9.4×10^(-6) and 0.00023 to 0.0009 respectively in both dermal and oral cases.,Multan Sadar(1.51,1.39)and Multan City(1.76,1.71)exhibited HI and HQ values that surpassed the threshold of 1.0,signaling a significant likelihood of chronic and cancer-causing health consequences for individuals who are exposed to polluted water causing *** findings suggest that the groundwater in the Multan district poses a significant health risk to humans due to high levels of arsenic *** contamination is associated with an increased risk of mortality from internal cancers(including liver,kidney,lung,and bladder),as well as a higher HI&CI incidence of skin ***,pregnancy outcomes are affected,with associations found between arsenic exposure and lower birth weight,and infant *** researchers propose that water supply organizations and educational institutions promptly take measures to ensure that the affected areas have access to arsenic-free drinking water for the well-being of students and residents.

关键词： Risk assessment Drinking water contamination Arsenic Primary school Pakistan

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Contrast Normalization Strategies in Brain Tumor Imaging:From Preprocessing to Classification

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Computer Modeling in Engineering & Sciences 2024年第8期140卷 1539-1562页

作者： Samar M.Alqhtani Toufique A.soomro Faisal Bin Ubaid ahmed Ali Muhammad Irfan Abdullah A.Asiri Department of Information Systems College of Computer Science and Information SystemsNajran UniversityNajran61441Saudi Arabia Department of Electronic Engineering The University of LarkanoLarkanaSindh75660Pakistan Computer Science Department Sukkur IBA UniversitySukkurSindh65200Pakistan Eletrical Engineering Department Sukkur IBA UniversitySukkurSindh65200Pakistan Electrical Engineering Department College of EngineeringNajran UniversityNajran61441Saudi Arabia Radiological Sciences Department College of Applied Medical SciencesNajran UniversityNajran61441Saudi Arabia

Cancer-related to the nervous system and brain tumors is a leading cause of mortality in various *** resonance imaging(MRI)and computed tomography(CT)are utilized to capture brain *** plays a crucial role in the diagnosis of brain tumors and the examination of other brain ***,manual assessment of MRI images by radiologists or experts is performed to identify brain tumors and abnormalities in the early stages for timely ***,early diagnosis of brain tumors is intricate,necessitating the use of computerized *** research introduces an innovative approach for the automated segmentation of brain tumors and a framework for classifying different regions of brain *** proposed methods consist of a pipeline with several stages:preprocessing of brain images with noise removal based on Wiener Filtering,enhancing the brain using Principal Component Analysis(PCA)to obtain well-enhanced images,and then segmenting the region of interest using the Fuzzy C-Means(FCM)clustering technique in the third *** final step involves classification using the Support Vector Machine(SVM)*** classifier is applied to various types of brain tumors,such as meningioma and pituitary tumors,utilizing the Contrast-Enhanced Magnetic Resonance Imaging(CE-MRI)*** proposed method demonstrates significantly improved contrast and validates the effectiveness of the classification framework,achieving an average sensitivity of 0.974,specificity of 0.976,accuracy of 0.979,and a Dice Score(DSC)of ***,this method exhibits a shorter processing time of 0.44 s compared to existing *** performance of this method emphasizes its significance when compared to state-of-the-art methods in terms of sensitivity,specificity,accuracy,and *** enhance the method further in the future,it is feasible to standardize the approach by incorporating a set of classifiers to increase the robustness of the brain classi

关键词： Brain tumor magnetic resonance imaging principal component analysis fuzzy c-clustering support vector machine

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影响MAX相合成及其性能的因素（英文）

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Science China Materials 2024年第11期67卷 3427-3455页

作者： Maaz Ullah Khan Sumair ahmed soomro Muhammad Irfan Jahanger 周延春楚珑晟冯庆国胡春峰 Key Laboratory of Advanced Technologies of Materials Ministry of EducationSchool of Materials Science and EngineeringSouthwest Jiaotong University School of Materials Science and Engineering Zhengzhou University

MAX相是一类三元化合物,具有层状晶体结构和混合化学键性质(共价-离子-金属键),因而其同时具有陶瓷和金属的特性.MAX相陶瓷展现出与其他传统陶瓷不同的独特性能.在本综述中,我们重点介绍了一些代表性的MAX相合成方法,这些方法具有成本... 详细信息

MAX相是一类三元化合物,具有层状晶体结构和混合化学键性质(共价-离子-金属键),因而其同时具有陶瓷和金属的特性.MAX相陶瓷展现出与其他传统陶瓷不同的独特性能.在本综述中,我们重点介绍了一些代表性的MAX相合成方法,这些方法具有成本效益和节能潜力.MAX相的纯度、杂质相的形成、微观结构和性能在制备过程中受到许多参数的影响.因此,我们特别强调了各种因素的影响,这些因素会交替削弱材料的制备效率和最终性能.本文总结了起始材料、化学计量比、温度、压力、粒度、孔隙率、微观结构、机械合金化、机械活化、离子辐照和掺杂等多个参数的影响,以揭示它们对MAX相合成和性能的影响.最后,我们探讨了MAX相在商业上的潜在应用,并展望其在工业领域的发展前景.

关键词： MAX phase ceramics synthesis route properties applications

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Lipase inhibitory activity of Lagenaria siceraria fruit as a strategy to treat obesity

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Asian Pacific Journal of Tropical Medicine 2017年第3期10卷 285-290页

作者： Maria maqsood Dildar ahmed Iqra Atique Wajeeha Malik Department of Chemistry Forman Christian College(A Chartered University)LahorePakistan

Objective:To explore pancreatic lipase inhibitory activity under different extraction conditions in order to track the most potent ***:The methanolic extract and its fractions in solvents of increasing polarity,ether,chloroform,ethyl acetate,n-butanol and water,were made through cold *** in ethanol,ethyl acetate,acetone and chloroform were similarly *** extract was prepared through hot decoction method.A reported method was used to determine lipase inhibitory activity of extracts and fractions over wide ranges of ***:The extracts and fractions exhibited concentration dependent *** IC50(μg/mL)values of methanolic,ethanolic,chloroform,ethyl acetate,acetone,ethyl acetate(after washing with water)and aqueous decoction were 293.40,266.47,157.59,182.12,352.34,257.00,and 190.00,*** activity of chloroform,ethyl acetate and aqueous extracts were close to that of the drug orlistat(IC50 146μg/mL).Out of the fractions of the methanolic extract,the chloroform fraction was most active(IC50 189.6μg/mL).The order of inhibitory activity of the fractions was as follows:chloroform>ether>n-butanolic>aqueous>ethyl *** GC/MS analysis of the most active chloroform faction showed the presence of hexadecanoic acid,methyl hexadecanoate,isopropyl palmitate,methyl 9,12-octadecadienate,and methyl 9,12,***:The study suggests that Lagenaria siceraria has potential to inhibit pancreatic lipase activity,suppressing lipid digestion and thereby diminishing entry of lipids into the *** intake of aqueous decoction of the fruit may therefore be recommended for control of *** acids and their esters may play role as inhibitors of lipase.

关键词： Lagenaria siceraria Obesity Lipase inhibitory activity Bottle gourd

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Investigating the Use of Email Application in Illiterate and Semi-Illiterate Population

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Computers, Materials & Continua 2020年第3期62卷 1473-1486页

作者： Sadeeq Jan Imran maqsood Salman ahmed Zahid Wadud Iftikhar Ahmad National Center for Cyber Security-UETP Department of CS&ITUniversity of Engineering&TechnologyPeshawarPakistan Department of Computer Software Engineering University of Engineering&TechnologyMardanPakistan Department of Computer Systems Engineering University of Engineering&TechnologyPeshawarPakistan 不详

The use of electronic communication has been significantly increased over the last few *** is one of the most well-known means of electronic *** email applications are widely used by a large population;however,illiterate and semi-illiterate people face challenges in using them.A major population of Pakistan is illiterate that has little or no practice of computer *** this paper,we investigate the challenges of using email applications by illiterate and semi-illiterate *** addition,we also propose a solution by developing an application tailored to the needs of illiterate/semi-illiterate *** shows that illiterate people are good at learning the designs that convey information with pictures instead of text-only,and focus more on one object/action at a *** proposed solution is based on designing user interfaces that consist of icons and vocal/audio instructions instead of ***,we use background voice/audio which is more helpful than flooding a picture with a lot of *** tested our application using a large number of users with various skill levels(from no computer knowledge to experts).Our results of the usability tests indicate that the application can be used by illiterate people without any training or third-party’s help.

关键词： Illiterate semi-illiterate email usability user interfaces

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Effects of Velocity-slip on the Flow and Heat Transfer of a Two- Dimensional Stagnation-point Flow Towards a Porous Stretching Sheet with Heat Generation/absorption

Effects of Velocity-slip on the Flow and Heat Transfer of a ...

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作者： soomro Feroz ahmed（苏莫）南京大学

学位级别：硕士

本文研究了作用在流上的两种速度滑移线(一阶速度滑移线及二阶速度滑移线)效应以及作用在不同可延展表面上二维滞点流的热传输效应。针对该问题的研究在工程应用领域具有重要意义,并已成为近期的热门研究方向。本文工作可分为四个部分... 详细信息

本文研究了作用在流上的两种速度滑移线(一阶速度滑移线及二阶速度滑移线)效应以及作用在不同可延展表面上二维滞点流的热传输效应。针对该问题的研究在工程应用领域具有重要意义,并已成为近期的热门研究方向。本文工作可分为四个部分。其中,第一部分用于介绍背景知识以及已有的基础研究文献。在第二部分,我们引入了描述该问题的数学模型,根据速度滑移线以及延展表面类型的不同,可将该问题分为三种场景。第三部则介绍离散方法及数值求解过程。数值结论和相关细节讨论在放在第四部分。本文引用的所有文献则见于文章末尾的附录部分。

关键词：可伸展板多孔介质相似变化打靶法部分滑移

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Bioinspired Multi-material Polyjet-printed Frog Robot for Synchronous and Asynchronous Swimming

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Journal of Bionic Engineering 2023年第3期20卷 923-933页

作者： Afaque Manzoor soomro Jae-Wook Lee Fida Hussain Memon Faheem ahmed Kashif Hussain Kyung Hyun Choi Department of Mechatronics Engineering Jeju National UniversityJeju63243South Korea Department of Electrical Engineering Sukkur IBA UniversitySukkur65200Pakistan

This paper proposes the bioinspired soft frog robot. All printing technology was used for the fabrication of the robot. Polyjet printing was used to print the front and back limbs, while ultrathin filament was used to print the body of the robot, which makes it a complete soft swimming robot. Dual thrust generation approach has been proposed by embedding the main muscle and antagonistic muscle in all the limbs, which enables it to attain high speed (18 mm/s), significant control to swim in dual mode (synchronous and asynchronous modes). To achieve the swimming motion of frog, four SMA (BMF 300) muscle wires were used. The frog robot is named as (FROBOT). The hind limbs are 60 mm long and 10 mm thick on average, while the front limbs are 35 mm long and 7 mm thick. Model-based design and rigorous analysis of the dynamics of real frogs have allowed FROBOT to be developed to swim at a level that is remarkably consistent with real frogs. Electrical and mechanical characteristics have been performed. In addition, the test data were further processed using TRACKER to analyze angle, displacement and velocity. FROBOT (weighs 65 g) can swim at different controllable frequencies (0.5–2 Hz), can rotate in any direction on command from custom built LabVIEW software allowing it to swim with speed up to 18 mm/s on deep water surface (100 cm) with excellent weight balance.

关键词： Bioinspired Soft robotics Frog FROBOT Asynchronous swimming

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Influence of Seed Priming on Performance and Water Productivity of Direct Seeded Rice in Alternating Wetting and Drying

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Rice science 2015年第4期22卷 189-196页

作者： Hafeez Ur REHMAN Muhammad KAMRAN Shahzad maqsood ahmed BASRA Irfan AFZAL Muhammad FAROOQ Department of Agronomy University of AgricultureFaisalabad Institute of Agriculture University of Western Australia College of Food and Agricultural Sciences King Saud University

Direct seeded rice is promising alternative to traditional transplanting, but requires appropriate crop and water management to maintain yield performance and achieve high water productivity. Present study evaluated the effect of seed priming and irrigation on crop establishment, tillering, agronomic traits, paddy yield, grain quality and water productivity of direct seeded rice in alternate wetting and drying （DSR-AWD） in comparison with direct seeded rice at field capacity （DSR- FC）. Seed priming treatments were osmo-priming with KCI （2.2%）, CaCI2 （2.2%） and moringa leaf extracts （MLE, 3.3%） including hydro-priming as control. Among the treatments, seed osmo-primed with MLE emerged earlier and had higher final emergence, followed by osmo-priming with CaCI2. Tillering emergence rate and number of tillers per plant were the highest for seed priming with CaCI2 in DSR- AWD. Total productive and non-productive tillers, panicle length, biological and grain yields, harvest index were highest for seed priming with MLE or CaCI2 in DSR-AWD. Similarly, grain quality, estimated in terms of normal grains, abortive and chalky grains, was also the highest in DSR-AWD with MLE osmo-priming. Benefit cost ratio and water productivity was also the highest in DSR-AWD for seed priming with MLE. In conclusion, seed priming with MLE or CaCI2 can be successfully employed to improve the direct seeded rice performance when practiced with alternate wetting and drying irrigation.

关键词： crop establishment grain filling rate seed priming water productivity yield direct seededrice alternating wetting and drying grain quality

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