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Development and application of a real-time polymerase chain reaction method for Campylobacter jejuni detection

World Journal of Gastroenterology 2013年第20期19卷 3090-3095页

作者： mao-jun zhang Bo Qiao Xue-Bin Xu Jian-Zhong zhang State Key Laboratory for Infectious Disease Prevention and Control and National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and Prevention Shanghai Municipal Center for Disease Control and Prevention

AIM:To develop a real-time polymerase chain reaction(PCR) method to detect and quantify Campylobacter jejuni(***) from stool ***:Primers and a probe for real-time PCR were designed based on the specific DNA sequence of the hipO gene in *** specificity of the primers and probe were tested against a set of Campylobacter *** other enteric *** optimal PCR conditions were determined by testing a series of conditions with standard a *** *** detection limits were obtained using purified DNA from bacterial culture and extracted DNA from the stool *** hundred and forty-two specimens were analyzed for the presence of *** by direct bacterial culture and real-time ***:The optimal PCR system was determined using reference DNA templates,1 × uracil-DNA glycosylase,3.5 mmol/L MgCl 2,1.25 U platinum Taq polymerase,0.4 mmol/L PCR nucleotide mix,0.48 μmol/L of each primer,0.2 μmol/L of probe and 2 μL of DNA template in a final volume of 25 μ*** PCR reaction was carried as follows:95 ℃ for 4 min,followed by 45 cycles of 10 s at 95 ℃ and 30 s at 59 ℃.The detection limit was 4.3 CFU/mL using purified DNA from bacterial culture and 10 3 CFU/g using DNA from stool ***(8.3%,20/242) *** strains were isolated from bacterial culture,while 41(16.9%,41/242) samples were found to be positive by realtime *** sequencing of the PCR product indicated the presence of *** in the *** mixed infection of *** and Salmonella was detected in one specimen and the PCR test for this specimen was ***:The sensitivity of detection of *** from stool specimens was much higher using this PCR assay than using the direct culture method.

关键词： Campylobacter jejuni Real time polymerase chain reaction Application

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Fabrication of a white organic light-emitting device with single liq:rubrence luminescent layer

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Optoelectronics Letters 2008年第2期4卷 126-129页

作者： CHEN Xiao-qiang WEI Wan-yin WU You-yu DONG mao-jun zhang Fu-jia School of Physical Science and Technology Lanzhou University Lanzhou 730000 China

A kind of white OLED with single luminescent layer was designed, in which rubrene was doped in Liq. The structure of the devices is ITO/PVK:TPD/Liq: Rubrene/Alq3/Al. The brightness of the devices comes to 3120 cd/m2(at a driving voltage of 25 V), the CIE coordinates of the typical devices is (0.308,0.347), and the coordinates is very close to the white equi-energy point. The emitting and luminescent characteristics of the devices were discussed.

关键词：白色有机光器件发光器件冷光散射

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Effect of Lactobacillus rhamnosus GG supernatant on serotonin transporter expression in rats with post-infectious irritable bowel syndrome

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World Journal of Gastroenterology 2018年第3期24卷 338-350页

作者： Ya-nan Cao Li-juan Feng Yuan-Yuan Liu Kui jiang mao-jun zhang Yi-xin Gu Bang-mao Wang jia Gao Ze-Lan Wang Yu-Ming Wang Department of Gastroenterology and Hepatology Tianjin Medical University General Hospital Department of Functional Division Xingtai People’s Hospital National Institute for Commu-nicable Disease Control and Prevention Chinese Center for Disease Control and Prevention

AIM To evaluate the effect of Lactobacillus rhamnosus GG supernatant(LGG-s) on the expression of serotonin transporter(SERT) in rats with post-infectious irritable bowel syndrome(PI-IBS).METHODS Campylobacter jejuni 81-176(1010 CFU/m L) was used to induce intestinal infection to develop a PI-IBS model. After evaluation of the post-infectious phase by biochemical tests, Dn A agarose gel electrophoresis, abdominal withdrawal reflex(AWR) test, and the intestinal motility test, four PI-IBS groups received different concentrations of LGG-s for 4 wk. The treatments were maintained for 1.0, 2.0, 3.0 or 4.0 wk during the experiment, and the colons and brains were removed for later use each week. SERT m Rn A and protein levels were detected by real-time PCR and Western blot, *** The levels of SERT m Rn A and protein in intestinal tissue were higher in rats treated with LGG-s than in control rats and PI-IBS rats gavaged with PBS during the whole study. Undiluted LGG-s up-regulated SERT m Rn A level by 2.67 times compared with the control group by week 2, and SERT m Rn A expression kept increasing later. Double-diluted LGG-s was similar to undiluted-LGG-s, resulting in high levels of SERT m Rn A. Triple-diluted LGG-s up-regulated SERT m Rn A expression level by 6.9-times compared with the control group, but SERT m Rn A expression decreased rapidly at the end of the second week. At the first week, SERT protein levels were basically comparable in rats treated with undiluted LGG-s, double-diluted LGG-s, and triplediluted LGG-s, which were higher than those in the control group and PBS-treated PI-IBS group. SERT protein levels in the intestine were also comparable in rats treated with undiluted LGG-s, double-diluted LGG-s, and triple-diluted LGG-s by the second and third weeks. SERT m Rn A and protein levels in the brain had no statistical difference in the groups during the *** LGG-s can up-regulate SERT m Rn A and protein levels in intestinal t

关键词： serotonin transporter intestinal infection Lactobacillus rhamnosus supernatant Irritable bowel syndrome

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Adherence and invasion of mouse-adapted H pylori in different epithelial cell lines

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World Journal of Gastroenterology 2007年第6期13卷 845-850页

作者： mao-jun zhang Fan-Liang Meng Xiao-Yun Ji Li-Hua He Jian-Zhong zhang Department of Diagnostics National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China Supported by the National Natural Science Foundation of China No. 30370078

AIM： To assess the adhesion and invasion abilities of different mouse adapted H py/or/strains in different cell lines in vitro and investigate their effects on the virulence factors cagA and vacA. METHODS： The adherence and invasion abilities of different N pylori strains in different epithelial cell lines were examined by the gentamycin protection assay. The null mutants of cagA and vacA were processed by direct PCR mutation method. The morphologic changes of different cell lines after N pylori attachment were examined by microscopy. RESULTS： The densities of adherence to and invasion into cells in vitro were different from those in the mouse infection experiments. 88-3887 strain could invade and adhere to cells stronger than SS1 and X47. All tested strains had better adhering and invasive abilities in SCG-7901 cell. CagA and vacA minus mutants had the same invasion and adherent abilities as their wild types. In all strains and cell lines tested, only AGS cell had the significant hummingbird phenotype after inoculation with the 88-3887 wild-type. CONCLUSION： Both the host cells and the bacteria play important parts in the invasion and adhesion abilities of Hpylori. CagA and VacA are not related to the ability of invasion and adhesion of Hpylori in different cell lines in vitro.

关键词： Hpylori Adherence Invasion Cell line

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Amino acid deletions at positions 893 and 894 of cytotoxinassociated gene A protein affect Helicobacter pylori gastric epithelial cell interactions

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World Journal of Gastroenterology 2024年第41期30卷 4449-4460页

作者： Zhi-Jing Xue Ya-Nan Gong Li-Hua He Lu Sun Yuan-Hai You Dong-Jie Fan mao-jun zhang Xiao-Mei Yan Jian-Zhong zhang Research Center of Translational Medicine Central Hospital Affiliated to Shandong First Medical UniversityJinan 250013Shandong ProvinceChina National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China

BACKGROUND Helicobacter pylori(***)persistently colonizes the human gastric mucosa in more than 50%of the global population,leading to various gastroduodenal diseases ranging from chronic gastritis to gastric ***-associated gene A(CagA)protein,an important oncoprotein,has highly polymorphic Glu-Pro-Ile-Tyr-Ala segments at the carboxyl terminus,which play crucial roles in *** previous study revealed a significant association between amino acid deletions at positions 893 and 894 and gastric *** To investigate the impact of amino acid deletions at positions 893 and 894 on CagA *** We selected a representative HZT strain from a gastric cancer patient with amino acid deletions at positions 893 and *** cagA gene was amplified and mutated into cagA-NT and cagA-NE(sequence characteristics of strains from nongastric cancer patients),cloned and inserted into pAdtrack-CMV,and then transfected into AGS *** expression of cagA and its mutants was examined using realtime polymerase chain reaction and Western blotting,cell elongation via cell counting,F-actin cytoskeleton visualization using fluorescence staining,and interleukin-8(IL-8)secretion via enzyme-linked immunosorbent *** The results revealed that pAdtrack/cagA induced a more pronounced hummingbird phenotype than pAdtrack/cagA-NT and pAdtrack/cagA-NE(40.88±3.10 vs 32.50±3.17,P<0.001 and 40.88±3.10 vs 32.17±3.00,P<0.001)at 12 hours after *** 24 hours,pAdtrack/cagA-NE induced significantly fewer hummingbird phenotypes than pAdtrack/cagA and pAdtrack/cagA-NT(46.02±2.12 vs 53.90±2.10,P<0.001 and 46.02±2.12 vs 51.15±3.74,P<0.001).The total amount of F-actin caused by pAdtrack/cagA was significantly lower than that caused by pAdtrack/cagA-NT and pAdtrack/cagA-NE(27.54±17.37 vs 41.51±11.90,P<0.001 and 27.54±17.37 vs 41.39±14.22,P<0.001)at 12 hours after ***,pAdtrack/cagA induced higher IL-8 secretion than pAdtrack/cagA-NT a

关键词： Cytotoxin-associated gene A Glu-Pro-Ile-Tyr-Ala Hummingbird phenotype Interleukin-8 Helicobacter pylori

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Interleukin-17A facilitates tumor progression via upregulating programmed death ligand-1 expression in hepatocellular carcinoma

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 176-198页

作者： Zhong-Xia Yang Li-Ting zhang Xiao-jun Liu Xue-Bin Peng Xiao-Rong mao The First School of Clinical Medicine Lanzhou UniversityLanzhou 730000Gansu ProvinceChina Department of Infectious Diseases The First Hospital of Lanzhou UniversityLanzhou 730000Gansu ProvinceChina Department of Radiotherapy Gansu Provincial HospitalLanzhou 730000Gansu ProvinceChina

BACKGROUND Hepatocellular carcinoma(HCC)is an inflammation-associated tumor with a dismal *** has become an important treatment strategy for HCC,as immunity is closely related to inflammation in the tumor *** regulates the expression of programmed death ligand-1(PD-L1)in the immunosuppressive tumor microenvironment and affects im-munotherapy ***-17A(IL-17A)is involved in the remodeling of the tumor microenvironment and plays a protumor or antitumor role in different *** hypothesized that IL-17A participates in tumor progression by affe-cting the level of immune checkpoint molecules in *** upregulation of PD-L1 expression in HCC cells by IL-17A was assessed by reverse transcription PCR,western blotting,and flow *** studies were conducted with gene knockout models and pathway *** function of IL-17A in immune evasion was explored through coculture of T cells and HCC *** effects of IL-17A on the malignant biological behaviors of HCC cells were evaluated in vitro,and the antitumor effects of an IL-17A inhibitor and its synergistic effects with a PD-L1 inhibitor were studied in *** IL-17A upregulated PD-L1 expression in HCC cells in a dose-dependent manner,whereas IL-17A receptor knockout or treatment with a small mothers against decapentaplegic 2 inhibitor diminished the PD-L1 expression induced by ***-17A enhanced the survival of HCC cells in the coculture ***-17A increased the viability,G2/M ratio,and migration of HCC cells and decreased the apoptotic *** D1,VEGF,MMP9,and Bcl-1 expression increased after IL-17A treatment,whereas BAX expression *** combination of IL-17A and PD-L1 inhibitors showed synergistic antitumor efficacy and increased cluster of differentiation 8+T lymphocyte infiltration in an HCC mouse *** IL-17A upregulates PD-L1 expression via the IL-17A receptor/phosphorylation-small mothers against

关键词： Interleukin-17A Programmed death ligand-1 Interleukin-17A receptor Small mothers against decapentaplegic 2 Hepatocellular carcinoma Immunotherapy

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不同工况下采煤机截齿截割阻力实验研究

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南京理工大学学报 2017年第2期41卷 159-164页

作者：李强毛君张明玉宿州学院机械与电子工程学院安徽宿州234000 辽宁工程技术大学机械工程学院辽宁阜新123000

针对采煤机在不同工况下的截齿截割阻力的变化特性及斜切进刀工况下事故频发的状况,该文设计了由贴应变片插入式测量截齿和无线信号采集模块等组成的截齿截割阻力在线实验方案。将该方案用于MG400/930-WD型采煤机,在某大型采掘装备研究... 详细信息

针对采煤机在不同工况下的截齿截割阻力的变化特性及斜切进刀工况下事故频发的状况,该文设计了由贴应变片插入式测量截齿和无线信号采集模块等组成的截齿截割阻力在线实验方案。将该方案用于MG400/930-WD型采煤机,在某大型采掘装备研究中心进行截割实验,得到了不同工况下采煤机截割阻力的变化曲线。分析实验结果可知,各截齿截割阻力在斜切进刀和正常工况下均为"间歇周期"变化,在单个周期内截割阻力先增大后减小,且增大和减少过程中呈波动变化;截割阻力在斜切进刀工况下先增大后减小,正常工况下较为稳定;在斜切进刀工况下,各截齿最大截割阻力数值及其出现的位置均不同,越晚参与截割的截齿出现最大截割阻力的位置越靠后,数值也越小,最大相差0.4 k N;斜切进刀工况下各截齿开始参与截割时,采煤机位置差异较大,最早与最晚位置相差约11.0 m;斜切进刀工况下的最大截割阻力达到6k N,约为正常工况下的6倍。该文实验方案可为采煤机斜切进刀工况下事故频发原因分析和截割部设计提供实验依据。

关键词：采煤机截割阻力实验斜切进刀

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Cross-reactivity of anti-H pylori antibodies with membrane antigens of human erythrocytes

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World Journal of Gastroenterology 2007年第27期13卷 3742-3746页

作者： Feng-Hua Guo Xiao-Mei Yan Chun-Xiang Fan Fei Zhao Yuan Hu Di Xiao Xun Zeng mao-jun zhang Li-Hua He Fan-Ling Meng Jian-Zhong zhang National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention PO Box 5 Changping Beijing 102206 China

AIM： To investigate whether anti-H pylori antibodies have cross-reaction with antigens of erythrocyte membrane. METHODS： Blood samples were collected from 14 volunteers （8 positive and 6 negative for Hpylori detected by ^13C-urea breath test） of the general population. Erythrocyte membrane proteins of the subjects were examined by Western blot using anti-H pylori serum. The proteins related to the positive bands were identified by mass spectrum analysis. RESULTS： Anti-Hpylori antibodies had cross-reaction with the proteins of about 50 kDa of erythrocyte membranes in all samples independent of H pylori infection. One protein in the positive band was identified as Chain S, the crystal structure of the cytoplasmic domain of human erythrocyte Band-3 protein. CONCLUSION： Anti-HpyloH antibodies cross-react with some antigens of human erythrocyte membrane, which may provide a clue for the relationship between Hpylori infection and vascular disorders.

关键词： H pylori Antibodies Erythrocyte Crossreactivity

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采煤机截齿三向力测试方案设计及实验研究

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工程设计学报 2016年第5期23卷 506-512页

作者：李强毛君张明玉谢苗宿州学院机械与电子工程学院安徽宿州234000 辽宁工程技术大学机械工程学院辽宁阜新123000

为研究采煤机工作状态下截齿负载特性,设计了截齿三向力测试方案,并应用该测试方案对MG400/930-WD型采煤机进行了相关实验研究,得到了采煤机截割某实验室模拟煤壁时的截齿三向力曲线,并对实验结果和理论计算结果进行了对比分析.考虑误... 详细信息

为研究采煤机工作状态下截齿负载特性,设计了截齿三向力测试方案,并应用该测试方案对MG400/930-WD型采煤机进行了相关实验研究,得到了采煤机截割某实验室模拟煤壁时的截齿三向力曲线,并对实验结果和理论计算结果进行了对比分析.考虑误差和随机因素的影响,可以认为实验结果与理论分析是一致的,验证了测试方案的可行性,能为采煤机截齿相关参数的改进及采煤机截割部的设计提供实验数据和理论依据.

关键词：采煤机截齿三向力测试方案测量截齿实验研究

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Lipid levels and insulin resistance markers in patients with colorectal cancer:Propensity score matching analysis

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 130-142页

作者： Ren-Hao Hu Dong-Yi Yan Ke-Hui zhang Di zhang Xi-mao Cui Xiao-Hua Jiang Shun zhang Department of Gastrointestinal Surgery Shanghai East HospitalSchool of MedicineTongji UniversityShanghai 200120China School of Medicine Tongji UniversityShanghai 200092China Department of Emergency Medicine Shanghai East HospitalSchool of MedicineTongji UniversityShanghai 200120China

BACKGROUND Colorectal cancer(CRC)is a prevalent malignant neoplasm characterized by subtle early *** To investigate the correlation among serum lipid profiles,the triglyceride-glucose(TyG)index,and the atherosclerotic index(AI)in patients with ***,it explored the clinical diagnostic utility of combining serum lipids with cancer antigens in the context of *** A retrospective analysis encompassed 277 patients with CRC and 1034 healthy *** Following propensity score matching,patients with CRC exhibited significantly reduced levels of serum triglyceride(TG),total cholesterol(TC),high-density lipoprotein cholesterol,and low-density lipoprotein cholesterol(LDL-C),as well as a diminished TyG ***,they displayed elevated AI levels compared to their healthy *** in advanced stages exhibited lower serum levels of TG,TC,and LDL-C compared to those in early *** with positive lymph node metastasis demonstrated reduced levels of TG,LDL-C,and the TyG *** operating characteristic analysis revealed that the combination of the TyG index,carcinoembryonic antigen,and carbohydrate antigen 19-9 yielded the highest positive prediction rate for CRC at 75.3%.CONCLUSION Preoperative serum lipid profiles exhibit a robust association with patients with *** concurrent assessment of multiple serum lipids and cancer antigens effectively enhances the diagnostic accuracy for CRC.

关键词： Colorectal cancer Serum lipids Triglyceride-glucose index Atherosclerotic index Diagnostic marker

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