Amino acid deletions at positions 893 and 894 of cytotoxinassociated gene A protein affect Helicobacter pylori gastric epithelial cell interactions
作者机构：Research Center of Translational MedicineCentral Hospital Affiliated to Shandong First Medical UniversityJinan 250013Shandong ProvinceChina National Key Laboratory of Intelligent Tracking and Forecasting for Infectious DiseasesNational Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文）)
年 卷 期：2024年第30卷第41期
页 面：4449-4460页
核心收录：
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：Supported by the Shandong Medical and Health Science and Technology Development Plan Project No.202202080452
主 题：Cytotoxin-associated gene A Glu-Pro-Ile-Tyr-Ala Hummingbird phenotype Interleukin-8 Helicobacter pylori
摘 要：BACKGROUND Helicobacter pylori(***)persistently colonizes the human gastric mucosa in more than 50%of the global population,leading to various gastroduodenal diseases ranging from chronic gastritis to gastric ***-associated gene A(CagA)protein,an important oncoprotein,has highly polymorphic Glu-Pro-Ile-Tyr-Ala segments at the carboxyl terminus,which play crucial roles in *** previous study revealed a significant association between amino acid deletions at positions 893 and 894 and gastric *** To investigate the impact of amino acid deletions at positions 893 and 894 on CagA *** We selected a representative HZT strain from a gastric cancer patient with amino acid deletions at positions 893 and *** cagA gene was amplified and mutated into cagA-NT and cagA-NE(sequence characteristics of strains from nongastric cancer patients),cloned and inserted into pAdtrack-CMV,and then transfected into AGS *** expression of cagA and its mutants was examined using realtime polymerase chain reaction and Western blotting,cell elongation via cell counting,F-actin cytoskeleton visualization using fluorescence staining,and interleukin-8(IL-8)secretion via enzyme-linked immunosorbent *** The results revealed that pAdtrack/cagA induced a more pronounced hummingbird phenotype than pAdtrack/cagA-NT and pAdtrack/cagA-NE(40.88±3.10 vs 32.50±3.17,P0.001 and 40.88±3.10 vs 32.17±3.00,P0.001)at 12 hours after *** 24 hours,pAdtrack/cagA-NE induced significantly fewer hummingbird phenotypes than pAdtrack/cagA and pAdtrack/cagA-NT(46.02±2.12 vs 53.90±2.10,P0.001 and 46.02±2.12 vs 51.15±3.74,P0.001).The total amount of F-actin caused by pAdtrack/cagA was significantly lower than that caused by pAdtrack/cagA-NT and pAdtrack/cagA-NE(27.54±17.37 vs 41.51±11.90,P0.001 and 27.54±17.37 vs 41.39±14.22,P0.001)at 12 hours after ***,pAdtrack/cagA induced higher IL-8 secretion than pAdtrack/cagA-NT a
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