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Natural Products and Bioprospecting 2016年第6期6卷 267-278页

作者： Mehtab Parveen Faheem Ahmad Ali Mohammed Malla Shaista Azaz Mahboob Alam Omer A.Basudan manuela ramos silva Pedro S.Pereira silva Department of Chemistry Aligarh Muslim UniversityAligarh 202002India Division of Bioscience Dongguk UniversityGyeongju 780-714Republic of Korea Pharmacognosy College of PharmacyKing Saud UniversityRiyadh 4451Saudi Arabia CFisUC Department of PhysicsUniversity of Coimbra3004-516 CoimbraPortugal

Phytochemical investigations of the ethanolic extract of leaves of Clutia lanceolata(Family:Euphorbiaceae)resulted in the isolation of four compounds viz.3,4-dihydroxy-2-methylbenzoic acid(1),2,20-dihydroxy-1,10-binaphthyl(2),1,3,8-trihydroxy-6-methylanthracene-9,10-dione(3)and 5-hydroxy-1,7-bis(4-hydroxy-3-methoxyphenyl)hepta-1,4,6-trien-3-one(4).Although all the isolated compounds were known but this was the first report from this plant *** structures were established on the basis of chemical and physical evidences *** analysis,FT-IR,1 H-NMR,13CNMR and mass spectral *** of compound 2 and 4 was further authenticated by single-crystal X-ray analysis and density functional theory *** isolated compounds(1–4)were screened for AChE enzyme inhibition assay in which compound 3 and 4 were found to be more potent AChE *** docking study of potent AChE inhibitor was performed to find the probable binding mode of the compounds into the active site of ***,the isolated compounds were also screened for in vivo cytotoxicity by brine shrimp lethality assay.

关键词： Clutia lanceolata X-ray crystallography AChE Molecular docking Cytotoxicity

Plant growth and metabolism of exotic and native Crotalaria species for mine land rehabilitation in the Amazon

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Journal of Forestry Research 2024年第1期35卷 143-154页

作者： Hemelyn Soares das Chagas Rafael silva Guedes Markus Gastauer Paula Godinho Ribeiro Allan Klynger da silva Lobato Cecílio Frois Caldeira Fabrício William deÁvila AndréRodrigues dos Reis Silvio Junio ramos Instituto Tecnológico Vale Rua Boaventura da Silva955BelémPA 66055-090Brazil Universidade Federal do Sul e Sudeste do Pará XinguaraPABrazil Núcleo de Pesquisa Vegetal Básica e Aplicada Universidade Federal Rural da AmazôniaParagominasPABrazil Departamento de Engenharia Florestal Universidade Estadual do Centro-Oeste/UNICENTROIratiPRBrazil School of Sciences and Engineering São Paulo State University(UNESP)TupãSP.CEP 17602-496Brazil

Despite its enormous benefits,mining is respon-sible for intense changes to vegetation and soil ***,after extraction,it is necessary to rehabilitate the mined areas,creating better conditions for the establishment of plant species which is *** study evaluated mineral and organic fertilization on the growth,and carbon and nitrogen(N)metabolism of two Crotalaria species[Cro-talaria spectabilis(exotic species)and Crotalaria maypu-rensis(native species from Carajás Mineral Province(CMP)]established on a waste pile from an iron mine in CMP.A control(without fertilizer application)and six fertilization mixtures were tested(i=NPK;ii=NPK+micronutrients;iii=NPK+micronutrients+organic compost;iv=PK;v=PK+micronutrients;vi=PK+micronutrients+organic compost).Fertilization contributed to increased growth of both species,and treatments with NPK and micronutrients had the best results(up to 257%***),while organic fertilization did not show *** Crotalaria had a greater number of nodules,higher nodule dry mass,chlorophyll a and b contents and showed free ammonium as the predominant N form,reflecting greater increments in biomass compared to native *** having lower growth,the use of this native species in the rehabilitation of mining areas should be considered,mainly because it has good development and meets current government legislation as an opportunity to restore local biodiversity.

关键词： Biological nitrogen fixation Mining Nitrogen metabolism Plant nutrition

Influence of broken kernels content on soybean quality during storage

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Grain & Oil Science and Technology 2024年第2期7卷 105-112页

作者： Lazaro da Costa Correa Canizares Cesar Augusto Gaioso Newiton da silva Timm Silvia Leticia Rivero Meza Adriano Hirsch ramos Maurício de Oliveira Everton Lutz Moacir Cardoso Elias Department of Agroindustry Science and Technology Federal University of PelotasPelotasRio Grande do Sul 96010-900Brazil Information and Communication Technological Axis Federal Institute of EducationScienceand Technology FarroupilhaCampus PanambiPanambiRio Grande do Sul 98280-000Brazil

Although it is recognized that the post-harvest system is most responsible for the loss of soybean quality,the real impact of this loss is still *** regulation allows 15%and 30%of broken soybean for group I and group II(quality groups),***,the industry is not informed about the loss in the quality parameters of soybeans and its impacts during long-term ***,the objective was to evaluate the effect of the breakage kernel percentage of soybean stored for 12 *** of 15% of breakage kernels did not affect soybean ***,content of 30% of breakage kernels affected significantly soybean quality,which was evidenced by the increase of up to 75% in moldy soybeans,72% in acidity,50% in leached solids,27% in electrical conductivity,and the decrease of up to 12% in soluble protein,6.4% in germination and 3.5% in thousand kernel weight after 8 months of *** the legislation establishes a percentage limit,it is recommended to store soybeans with up to 15% breakage *** the contrary,values higher than that can cause a significant reduction in soybean quality,resulting in commercial losses.

关键词： Soybean quality Breakage kernels Storage problems Grain defects Quality parameters

History Teacher Training from Decolonial Curriculum Practices to Combat Racism at UniProjeção in the Federal District-A Secondary Publication

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Journal of Contemporary Educational Research 2024年第2期8卷 187-202页

作者： Karoline da silva ramos Cláudia Pinheiro Nascimento Francisca Carla Santos Ferrer Faculty of Philosophy Languages and Human SciencesUniversity of São PauloSão PauloBrazil

This article aims to understand the training process of history undergraduates,to see if there are decolonial curricular practices to combat racism at the Centro Universitário e Faculdade Projeção(UniProjeção)in the Federal District,to understand how coloniality has corroborated the exclusion of different epistemologies and the erasure of different cultures,and how this exclusionary process of coloniality interferes in the training of history *** order to combat this practice,we are looking for alternatives that can break these suppressions carried out by *** this way,we turn to decolonial ideas that aim to break with the logic of *** can conclude that these practices are poorly developed in the institution,so we proposed active problem-based methodology and music as a didactic *** playful educational tools that strengthen the teaching-learning process,they are active agents in the decolonial work of combating racism,and it is essential to train responsible and ethical teachers in the fight against racism and any form of oppression.

关键词： Coloniality Decoloniality Education Curriculum practices Racism

How far have we come?Review of main public policies to reduce landslide impacts in Brazil

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Journal of Mountain Science 2024年第9期21卷 2891-2904页

作者： Bianca Carvalho VIEIRA Tiago Damas MARTINS Telma Mendes da silva JoséEduardo BONINI Department of Geography University of São PauloAvenida Professor Lineu Prestes 05508-000Brazil Cities Institute Federal University of São PauloAvenida Jacu-Pêssego 08260-001Brazil Department of Geography Federal University of Rio de JaneiroAvenida Athos da Silveira Ramos 21941-916Brazil

During the rainy season in Brazil,landslides can have catastrophic consequences,including loss of life,damage to urban infrastructure and significant economic ***,more than eight million people in Brazil live in disaster-prone *** objective of this article is to present a chronological review of the principal policies and programmes at the national,state,and municipal levels,with an emphasis on their key actions aimed at mitigating the impact of disasters related to natural hazards,particularly *** analysis was based on official records of public policies from the national,state,and municipal *** results show that several initiatives were initially developed at the local and regional ***,it was not until the late 1980s that a comprehensive organisation of civil protection at the national level emerged as a response *** is possible to highlight three phases(understanding,coexistence,and prevention)of institutional action focused on risk management in the face of *** the growing knowledge of risk scenarios,disasters continue to occur in Brazil on an annual basis,revealing several obstacles to reducing their impact,particularly given the social and economic disparities between Brazilian regions and the applicability of public policies that must overcome the limitations of each municipality.

关键词： Disasters Risk Mitigation Prevention Legislation Civil Defense Natural Hazards

Enhancement of the Antigenotoxic and Antioxidant Actions of Eugenol from Spice Clove and the Stabilizer Gum Arabic on Colorectal Carcinogenesis

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Food and Nutrition Sciences 2024年第1期15卷 71-100页

作者： Nayanna de Oliveira ramos Melo Lucas Gabriel da Costa Marques Humberto Maia Costa Neto Matheus De Sousa silva Francisco Vagnaldo Fechine Jamacaru Bruno Coêlho Cavalcanti Antônio Adailson De Sousa silva Conceição Aparecida Dornelas Postgraduate Program in Medical-Surgical Sciences School of Medicine Federal University of Ceará Fortaleza Brazil School of Medicine Federal University of Ceará Fortaleza Brazil Nucleus of Research and Development of Medicines (NPDM) Laboratory of Pharmacology and Preclinical Research School of Medicine Federal University of Ceará Fortaleza Brazil Nucleus for Research and Development of Medicines (NPDM) National Laboratory of Experimental Oncology Federal University of Ceará Fortaleza Brazil Postgraduate Program in Pathology and Medical-Surgical Sciences School of Medicine Federal University of Ceará Fortaleza Brazil

Spices are defined as any aromatic condiment of plant origin used to alter the flavor and aroma of foods. Besides flavor and aroma, many spices have antioxidant activity, mainly related to the presence in cloves of phenolic compounds, such as flavonoids, terpenoids and eugenol. In turn, the most common uses of gum arabic are in the form of powder for addition to soft drink syrups, cuisine and baked goods, specifically to stabilize the texture of products, increase the viscosity of liquids and promote the leavening of baked products (e.g., cakes). Both eugenol, extracted from cloves, and gum arabic, extracted from the hardened sap of two species of the Acacia tree, are dietary constituents routinely consumed virtually throughout the world. Both of them are also widely used medicinally to inhibit oxidative stress and genotoxicity. The prevention arm of the study included groups: Ia, IIa, IIIa, Iva, V, VI, VII, VIII. Once a week for 20 weeks, the controls received saline s.c. while the experimental groups received DMH at 20 mg/kg s.c. During the same period and for an additional 9 weeks, the animals received either water, 10% GA, EUG, or 10% GA + EUG by gavage. The treatment arm of the study included groups Ib, IIb, IIIb e IVb, IX, X, XI, XII). Once a week for 20 weeks, the controls received saline s.c. while the experimental groups received DMH at 20 mg/kg s.c. During the subsequent 9 weeks, the animals received either water, 10% GA, EUG or 10% GA + EUG by gavage. The novelty of this study is the investigation of their use alone and together for the prevention and treatment of experimental colorectal carcinogenesis induced by dimethylhydrazine. Our results show that the combined use of 10% gum arabic and eugenol was effective, with antioxidant action in the colon, as well as reducing oxidative stress in all colon segments and preventing and treating genotoxicity in all colon segments. Furthermore, their joint administration reduced the number of aberrant crypts and th

关键词： Eugenol Gum Arabic Carcinogenesis Oxidative Stress Genotoxicity

A Novel and Functional Artificial Bruch’s Membrane Made of Poly (ε-Caprolactone)/Gelatin for Retinal Pigment Epithelium Restoration

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Advances in Bioscience and Biotechnology 2024年第8期15卷 443-461页

作者： López Edith silva-Bermudez Phaedra Gonzalez-Salinas Roberto Velasquillo Cristina Torres-Romero Abigail López-Colomé Ana María Pérez Armando Gina Prado-Prone Edgar ramos-Martínez Iván ramos-Martínez Quiroz-Mercado Hugo Hernández-Zimbrón Luis Fernando Instituto de Fisiologa Celular Universidad Nacional Autnoma de Mxico Ciudad de Mxico City Mxico Unidad de Ingeniería de Tejidos Terapia Celular y Medicina Regenerativa Instituto Nacional de Rehabilitación Luis Guiller-mo Ibarra Ibarra Ciudad de México México Departamento de Investigacin Asociacin Para Evitar la Ceguera Ciudad de Mxico Mxico Subdireccin de Investigacin Tecnolgica Instituto Nacional de Rehabilitacin Luis Guillermo Ibarra Ibarra Ciudad de Mxico Mxico Departamento de Biologa Celular y Tisular Facultad de Medicina Universidad Nacional Autnoma de Mxico Ciudad de Mxico Mxico Escuela de Ciencias Universidad Autnoma Benito Jurez de Oaxaca Oaxaca Mxico Departamento de Medicina y Zootecnia de Cerdos Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional utnoma de Mxico Mxico Laboratorio de Investigacion Interdisciplinaria rea de Optometria Escuela Nacional de Estudios Superiores Unidad Len Universidad Nacional Autnoma de Mxico Len Guanajuato Mxico Laboratorio Nacional de ptica de la Visin-Centro de Investigaciones en ptica Len Guanajuato Mxico

Purpose: The primary aim of this paper is to design, create, and improve functional and artificial Bruch’s membranes (BM) using bioengineering techniques, which can be applied in the treatment of maculopathies by supporting the growth and maintenance of retinal pigment epithelium (RPE) cells, thereby potentially enabling subretinal implantation in patients. Methods: We fabricated by electrospinning ultrathin 3D nanofibrous membranes from Polycaprolactone (PCL), and different concentrations of gelatin (5%, 15% and 30%). ARPE-19 cells were seeded onto these artificial membranes. MTT assays were performed in order to evaluate ARPE-19 cell viability and cytotoxicity. IF assays were realized to observe the ARPE-19 cells onto each membrane. Ultrastructure of the modified Bruch’s membrane and ARPE-19 morphology after 25 days of culture were studied with transmission and scanning electron microscopy. To evaluate expression changes in markers of ARPE-19 (RPE65 and ZO-1) qRT-PCR assays were realized. Data from three independent experiments were pooled and expressed as the mean SD. A confidence level of P 0.05 was considered to be statistically significant. Results ARPE-19 cells grew on PCL/Gelatin membranes mainly in PCL/30% gelatin combination, which had not cytotoxic effect. RPE65/cytokeratin-18/ and actin-positive ARPE-19 cells formed a correctly orientated monolayer of polygonal cells with morphological polarity. The apical cell surfaces exhibited abundant protruding microfolds. Moreover, zones of polygonal border look as if ARPE-19 cells fused among, suggesting the presence of tight junctions. The expression of RPE65 and ZO-1 was unchanged. Conclusions: PCL/30% Gelatine membranes may imitate the natural BM to such extent that they support RPE-cells and exhibited RPE-like morphology. The engineering of a human RPE monolayer with these artificial BM, emulating the in vivo retina, arouse their potential subretinal implantation in patients with wet age-related macular degene

关键词： Artificial Bruch’s Membrane Electrospinning ARPE-19 Cells Age-Related Macular Degeneration

Ingredients from integral valorization of Isabel grape to formulate goat yogurt with stimulatory effects on probiotics and beneficial impacts on human colonic microbiota in vitro

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Food Science and Human Wellness 2023年第4期12卷 1331-1342页

作者： Francyeli Araújo silva Rita de Cássia ramos do Egypto Queiroga Evandro Leite de Souza Glenise Bierhalz Voss Maria manuela Estevez Pintado Margarida Angélica da silva Vasconcelos Department of Nutrition Federal University of PernambucoRecife 50670-420Brazil Department of Nutrition Health Sciences CenterFederal University of ParaíbaJoão Pessoa 58050-585Brazil Universidade Católica Portuguesa CBQF-Centro de Biotecnologia e Química Fina-Laboratório AssociadoEscola Superior de BiotecnologiaPorto 4169-005Portugal

Isabel grape(IG)products have high contents of phenolic compounds and fiber recognized for their positive impacts on microorganisms associated with health benefits to *** study evaluated the effects of goat yogurts formulated with ingredients from IG integral valorization on the growth and metabolism of different probiotic strains,as well as on the population of selected bacterial groups and metabolic activity of human colonic microbiota in *** yogurts with IG ingredients(IGI)stimulated the growth of tested Lactobacillus and Bifidobacterium probiotic strains during a 48-h cultivation,as well as decreased the pH values and enhanced the organic acid *** yogurts with IGI increased the population of Lactobacillus *** Bifidobacterium *** a 24-h in vitro colonic fermentation.A stable Firmicutes:Bacteroidetes ratio close to 1 was found in media with goat yogurt formulations during the colonic fermentation,being similar to the effect caused by *** yogurt formulations with IGI caused increased production of short-chain fatty acids and sugar consumption during colonic *** yogurts with IGI should be a valuable strategy for development of novel added-value foods with beneficial effects on gut microbiota and human health.

关键词： Goat dairy product Grape Beneficial microorganisms Gut microbiota Modulatory effects

Recognition of Human Actions through Speech or Voice Using Machine Learning Techniques

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Computers, Materials & Continua 2023年第11期77卷 1873-1891页

作者： Oscar Peña-Cáceres Henry silva-Marchan manuela Albert Miriam Gil Professional School of Systems Engineering Universidad César VallejoPiura20009Perú Escola Tècnica Superior d’Enginyeria Departament d’InformàticaUniversitat de ValènciaBurjassotValencia46100Spain Department of Mathematics Statistics and InformaticsUniversidad Nacional de TumbesTumbes24000Perú Valencian Research Institute for Artificial Intelligence Universitat Politècnica de ValènciaValencia46022Spain

The development of artificial intelligence(AI)and smart home technologies has driven the need for speech recognition-based *** demand stems from the quest for more intuitive and natural interaction between users and smart devices in their *** recognition allows users to control devices and perform everyday actions through spoken commands,eliminating the need for physical interfaces or touch screens and enabling specific tasks such as turning on or off the light,heating,or lowering the *** purpose of this study is to develop a speech-based classification model for recognizing human actions in the smart *** seeks to demonstrate the effectiveness and feasibility of using machine learning techniques in predicting categories,subcategories,and actions from sentences.A dataset labeled with relevant information about categories,subcategories,and actions related to human actions in the smart home is *** methodology uses machine learning techniques implemented in Python,extracting features using CountVectorizer to convert sentences into numerical *** results show that the classification model is able to accurately predict categories,subcategories,and actions based on sentences,with 82.99%accuracy for category,76.19%accuracy for subcategory,and 90.28%accuracy for *** study concludes that using machine learning techniques is effective for recognizing and classifying human actions in the smart home,supporting its feasibility in various scenarios and opening new possibilities for advanced natural language processing systems in the field of AI and smart homes.

关键词： AI machine learning smart home human action recognition

Parallel evolution of fish bi-modal breathing and expansion of olfactory receptor(OR)genes:toward a universal ORs nomenclature

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遗传学报:英文版 2023年第8期50卷 600-610页

作者： Liliana silva Tito Mendes Luana ramos Guojie Zhang Agostinho Antunes CIIMAR/CIMAR Interdisciplinary Centre of Marine and Environmental ResearchUniversity of PortoTerminal de Cruzeiros do Porto de LeixõesAv.General Norton de Matoss/n4450-208PortoPortugal Department of Biology Faculty of SciencesUniversity of PortoRua do Campo Alegre4169-007PortoPortugal Villum Centre for Biodiversity Genomics Section for Ecology and EvolutionDepartment of BiologyUniversity of CopenhagenDK-2100 CopenhagenDenmark BGI-Shenzhen Beishan Industrial ZoneShenzhenGuangdong 518083China State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China

Olfactory receptors(ORs)play a key role in the prime sensorial perception,being highly relevant for intra/interspecific *** are a subgroup of G-protein coupled receptors that exhibit highly complex subgenomes in ***,OR repertoires remain poorly studied in fish lineages,precluding finely retracing their origin,evolution,and diversification,especially in the most basal ***,we conduct an exhaustive gene screening upon 43 high-quality fish genomes exhibiting varied gene repertoires(2e583 genes).While the early vertebrates performed gas exchange through gills,we hypothesize that the emergence of new breathing structures(swim bladder and paired lungs)in early osteichthyans may be associated with expansions in the ORs gene families sensitive to airborne ***,we verify that the OR repertoire of moderns actinopterygians has not increased as expected following a whole genome duplication,likely due to regulatory mechanisms compensating the gene load ***,we identify 25 distinct OR families,allowing us to propose an updated universal nomenclature for the fish ORs.

关键词： Fish OR repertoires Updated ORs nomenclature ORs expansion Bi-modal breathing WGD and ORs diversification