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Oxidative stress and mitochondrial transfer:A new dimension towards ocular diseases

Genes & Diseases 2022年第3期9卷 610-637页

作者： Mohana Devi Subramaniam Mahalaxmi Iyer Aswathy P.Nair Dhivya Venkatesan Sinnakaruppan Mathavan Nimmisha Eruppakotte Soumya Kizhakkillach manoj kumar Chandran Ayan Roy Abilash Valsala Gopalakrishnan Balachandar Vellingiri SN ONGC Department of Genetics and Molecular Biology Vision Research FoundationChennai 600006Tamil NaduIndia Department of Zoology Avinashilingam Institute for Home Science and Higher Education for WomenCoimbatore 641043Tamil NaduIndia Human Molecular Cytogenetics and Stem Cell Laboratory Department of Human Genetics and Molecular BiologyBharathiar UniversityCoimbatore 641046Tamil NaduIndia Department of Biotechnology Lovely Professional UniversityPunjab 144411India Department of Biomedical Sciences School of Bio Sciences and Technology(SBST)Vellore Institute of Technology(VIT)Vellore 600127India

Ocular cells like,retinal pigment epithelium(RPE)is a highly specialized pigmented monolayer of post-mitotic cells,which is located in the posterior segment of the eye between neuro sensory retina and vascular *** functions as a selective barrier and nourishes retinal visual *** a result of high-level oxygen consumption of retinal cells,RPE cells are vulnerable to chronic oxidative stress and an increased level of reactive oxygen species(ROS)generated from *** oxidative stress and ROS generation in retinal cells lead to RPE *** sources including mtDNA damage could be an important factor of oxidative stress in *** therapy and mitochondrial transfer studies are emerging fields in ocular disease *** retinal degenerative diseases stem cell-based transplantation methods are developed from basic research to preclinical and clinical *** research contributions of gene and cell therapy would be a new strategy to prevent,treat and cure various ocular *** review focuses on the effect of oxidative stress in ocular cell degeneration and recent translational researches on retinal degenerative diseases to cure blindness.

关键词： Mitochondrial transfer Oxidative stress Retinal pigment epithelium(RPE) RPE Degeneration Stem cell treatment

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An Improved Methodology to Overcome Key Issues in Human Fecal Metagenomic DNA Extraction

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Genomics, Proteomics & Bioinformatics 2016年第6期14卷 371-378页

作者： Jitendra kumar manoj kumar Shashank Gupta Vasim Ahmed Manu Bhambi Rajesh Pandey Nat Singh Chauhan Department of Biochemistry Maharshi Dayanand UniversityRohtak 124001India Ayurgenomics Unit Translational Research and Innovative Science Through AyurgeomicsCouncil of Scientific and Industrial ResearchInstitute of Genomics and Integrative BiologyNew Delhi 110020India

Microbes are ubiquitously distributed in nature, and recent culture-independent studies have highlighted the significance of gut microbiota in human health and disease. Fecal DNA is the primary source for the majority of human gut microbiome studies. However, further improvement is needed to obtain fecal metagenomic DNA with sufficient amount and good quality but low host genomic DNA contamination. In the current study, we demonstrate a quick, robust, unbiased, and cost-effective method for the isolation of high molecular weight （〉 23 kb） metagenomic DNA （260/280 ratio 〉 1.8） with a good yield （55.8 ± 3.8 ng/mg of feces）. We also confirm that there is very low human genomic DNA contamination （eubacterial： human genomic DNA marker genes = 227.9：1） in the human feces. The newly-developed method robustly performs for fresh as well as stored fecal samples as demonstrated by 16S rRNA gene sequencing using 454 FLX＋. Moreover, 16S rRNA gene analysis indicated that compared to other DNA extraction methods tested, the fecal metagenomic DNA isolated with current methodology retains species richness and does not show microbial diversity biases, which is further confirmed by qPCR with a known quantity of spike-in genomes. Overall, our data highlight a protocol with a balance between quality, amount, user-friendliness, and cost effectiveness for its suitability toward usage for cultureindependent analysis of the human gut microbiome, which provides a robust solution to overcome key issues associated with fecal metagenomic DNA isolation in human gut microbiome studies.

关键词： Metagenomic DNAextraction Gut microbiome Human feces 16S rRNA

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Experimental study on spraying performance of biological pesticides in aerial rotary cage nozzle

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International Journal of Agricultural and Biological Engineering 2020年第6期13卷 1-6页

作者： Yu Ru Yangyang Liu Rongjia Qu manoj kumar Patel Collage of Mechanical and Electrical Engineering Nanjing Forestry UniversityNanjing 210037China CSIR-Central Scientific Instruments Organisation Chandigarh 160030India Academy of Scientific and Innovative Research AcSIR-CSIOChandigarh 160030India

Aviation spraying is one of the most important methods for the treatment of forestry pests and diseases,which has a high operation efficiency and *** combination of aviation spray and biological pesticides to reduce the impact of pesticide spray on environment has become an important development in the direction of agricultural *** order to explore the effects of aviation spray parameters on particle size distribution and activity retention of biological pesticide droplets,a system of droplets size test and an activity retention rate test system of biological pesticides were *** related experiments were conducted by changing the structural parameters of the rotary cage nozzle(the diameter of nozzle)and the technical parameters of the operation(rotary speed and flow rate).The results showed that the average relative width S of the droplets of the rotary cage nozzle was close to 1,and the droplet distribution was *** influence factors on the particle size distribution of biological pesticide droplets were rotary speed,nozzle diameter,medium and flow rate in descending *** rotary speed,nozzle diameter,and medium had a significant influence on droplet size *** average activity retention rate of Bacillus thuringiensis was 88.83%,and the germination rate of Beauveria bassiana was 89.80%.The rotary speed had a significant effect on the activity retention rate of bacterial and fungal biological pesticides,and it was negatively *** rotary speed was between 3000-5000 r/min,and the activity retention rate was *** research results can provide guidance for spraying biological pesticides in aerial plant protection operations with rotary cage nozzle.

关键词： forestry disease and pest control biological pesticide activity retention rate aerial rotary cage nozzle droplet distribution

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An interval type-2 fuzzy logic controller for TCSC to improve the damping of power system oscillations

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Frontiers in Energy 2013年第3期7卷 307-316页

作者： manoj kumar PANDA Gopinath PILLAI Vijay kumarx Electronics & Computer Engineering Department Indian Institute ofTechnology Roorkee 247667 India Electrical Engineering Department Indian Institute of TechnologyRoorkee 247667 India

In this paper an interval type-2 fuzzy logic controller （IT2FLC） was proposed for thyristor controlled series capacitor （TCSC） to improve power system damping. For controller design, memberships of system variables were represented using interval type-2 fuzzy sets. The three-dimensional membership function of type-2 fuzzy sets provided additional degree of freedom that made it possible to directly model and handle uncertainties. Simulations conducted on a single machine infinite bus （SMIB） power system showed that the proposed controller was more effective than particle swarm optimization （PSO） tuned and type-1 fuzzy logic （T1FL） based damping controllers. Robust performance of the proposed controller was also validated at different operating conditions, various disturbances and parameter variation of the transmission line parameters.

关键词： power system oscillations thyristor controlled series capacitor (TCSC) type-2 fuzzy logic system interval type-2 fuzzy logic controller (IT2FLC)

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EUS-guided Gall Bladder Drainage in Severe Liver Disease: A Single-center Experience in Critically Ill Cirrhotics

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Journal of Clinical and Translational Hepatology 2018年第1期6卷 35-39页

作者： Kapil Dev Jamwal manoj kumar Sharma Rakhi Maiwall Barjesh kumar Sharma Shiv kumar Sarin Department of Hepatology Institute of Liver and Biliary SciencesD1Vasant KunjNew DelhiIndia Department of Hepatology Institute of Liver and Biliary SciencesD1Vasant KunjNew Delhi 110070India

Background and Aims:Acute calculous cholecystitis with impending gall bladder perforation in severe liver diseases including decompensated cirrhosis and acute-on-chronic liver failure(ACLF)is difficult to manage,due to the procedures such as cholecystectomy and percutaneous cholecystostomy being associated with high risk and complications in these ***:Four cases of severe liver disease with acute calculous cholecystitis who presented to the Institute of Liver and Biliary Sciences(New Delhi,India)for further management were included in the study if they underwent endoscopic ultrasound-guided gall bladder drainage(EUS-GBD).The patients were followed up for a minimum of 3 months and outcomes were ***:Four cases of severe liver disease(three ACLF and one decompensated cirrhosis),with model for end-stage liver disease scores of 24,26,23 and 25 respectively,presented with acute calculous cholecystitis(Tokyo grade III)and systemic sepsis(high total leukocyte counts).Their international normalized ratios were 2.3,2.6,2.2 and 2.9 respectively,and two were in shock,requiring inotropes at *** of the abdomen confirmed hugely distended gall bladder with stone impacted at the neck and moderate *** these cases underwent EUS-GBD by linear echo endoscope,and had the gastric wall punctured in the antrum using a 19G access needle followed by dilatation of the tract using controlled radial expansion balloon and Sohendra *** three cases,the plastic stents were *** the fourth case,a Nagi stent was *** the patients recovered and were discharged within a ***:EUS-GBD is challenging in severe liver disease but represents a life-saving procedure,and hence can be attempted in such critically ill patients with utmost care and precaution.

关键词： ACLF Acute-on-chronic liver failure Cholecystitis Ascites

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MicroRNA-99a mimics inhibit M1 macrophage phenotype and adipose tissue inflammation by targeting TNFα

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Cellular & Molecular Immunology 2019年第5期16卷 495-507页

作者： Anant Jaiswal Sukka Santosh Reddy Mohita Maurya Preeti Maurya manoj kumar Barthwal Division of Pharmacology Council of Scientific and Industrial Research-Central Drug Research Institute(CSIR-CDRI)Lucknow 226031 U.P.India Academy of Scientific and Innovative Research New Delhi 110025India

In human adipose tissue and obesity,miR-99a expression is negatively correlated with ***,the present study investigated the role of miR-99a in macrophage phenotype activation and adipose tissue inflammation.M2 BMDMs showed a significant increase in miR-99a expression when compared to the M0 and M1 ***-switching experiments established an association between upregulated miR-99a expression and the M2 *** of miR-99a prevented M1 phenotype activation and attenuated bactericidal ***,knockdown of miR-99a abolished M2 phenotype *** means of in silico target prediction tools and a luciferase reporter assay,TNFαwas identified as a direct target of *** of TNFαrecapitulated the effect of miR-99a overexpression in M1 *** a db/db mice model,miR-99a expression was reduced in eWAT and F4/80+*** overexpression of miR-99a in db/db mice attenuated adipocyte hypertrophy with increased CD301 and reduced CD86 *** cytometry analysis also showed an increased M2 and a reduced M1 macrophage *** of miR-99a also improved the diabetic dyslipidemia and insulin signaling in eWAT and liver,with an attenuated expression of gluconeogenesis and cholesterol metabolism genes in the ***,adoptive transfer of miR-99a-overexpressing macrophages in the db/db mice recapitulated in vivo miR-99a mimic effects with increased M2 and reduced M1 macrophage populations and improved systemic glucose,insulin sensitivity,and insulin signaling in the eWAT and *** present study demonstrates that miR-99a mimics can regulate macrophage M1 phenotype activation by targeting TNFα.miR-99a therapeutics in diabetic mice reduces the adipose tissue inflammation and improves insulin sensitivity.

关键词： inflammation macrophage CD86

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Mechanical Properties and Microstructural Evolution of Bulk UFG Al 2014 Alloy Processed Through Cryorolling and Warm Rolling

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Acta Metallurgica Sinica(English Letters) 2019年第7期32卷 845-856页

作者： manoj kumar Pathak Amit Joshi K. K. S. Mer R. Jayaganthan Department of Mechanical Engineering G. B. Pant Institute of Engineering and Technology Pauri-Garhwal 246194 India Department of Engineering Design. Indian Institute of Technology Madras Chennai 600036 India

Tensile properties, microstructural evolution and fracture toughness of Al 2014 alloy subjected to cryorolling followed by warm rolling (CR + WR) have been investigated in the present study. The solution-treated (ST) Al 2014 alloy is cryorolled followed by warm rolling process at different temperatures (110, 170 and 210 ℃). The mechanical properties and microstructural features of deformed and undeformed Al 2014 alloys were characterised by optical microscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The CR + WR samples at 170 ℃ showed an improved hardness (179 HV), tensile (UTS 499 MPa, YS 457 MPa) and fracture toughness (KQ = 37.49 MPa y/m, Kee = 37.39 MPa √m and J integral = 33.25 kJ/mm^2) with respect to ST alloy as measured from the tensile and fracture toughness test. The improved mechanical properties of CR + WR alloy are attributed to grain boundary strengthening, combined recovery and recrystallisation, precipitation hardening and dynamic ageing effect during the deformation. The precipitation of metastable spherical phase Al2Cu was responsible for the improved tensile and fracture properties of finegrained Al 2014 alloy observed in the present work.

关键词： Al alloys Ultrafine grains Fracture toughness

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Assessment of Genetic Diversity of Drought Tolerant and Susceptible Rice Genotypes Using Microsatellite Markers

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Rice science 2019年第4期26卷 239-247,I0005,I0010-I0014页

作者： Ravindra DONDE Jitendra kumar Gayatri GOUDA manoj kumar GUPTA Mitadru MUKHERJEE Sk Yasin BAKSH Pradosh MAHADANI Khirod kumar SAHOO Lambodar BEHERA Sushanta kumar DASH Crop Improvement Division Indian Council of Agricultural Research-National Rice Research Institute/Formerly-Central Rice Research Institute Cuttack Pin-753006.OdishaIndia Ravenshaw University Department of Biotechnology Cuttack Pin-753003Odisha India Department of Biotechnology & Bioinformatics Yogi Vemana University KadapaPin-516003 OdishaIndia

The introgression of wild chromosomal segments into popular rice varieties is one of the potential approaches for developing varieties for drought stress condition. Sixteen genotypes, including nine indica, two tropical japonica and five chromosome segments substitution lines (CSSLs) with different levels of tolerance/susceptibility to drought stress, were selected for diversity study. Sixty-three microsatellite markers were utilized for assessing genetic diversity. A total of 95 alleles were amplified, and out of them, 60 were polymorphic. Six unique alleles, amplified by the microsatellite loci RM276, RM472, RM488, RM537, RM541 and RM28089, were identified in six genotypes, namely FR13A, Brahamanakhi, RUF44, Swarna-sub1, Brahamanakhi and Satyabhama. The highest genetic similarity was found among CSSLs. Polymorphism information content (PIC) value varied from 0 to 1.00 with an average of 0.66 per locus. Twenty-eight microsatellites were found to be polymorphic, which could be used in marker-assisted selection programme. All the sixteen genotypes were grouped into two major clusters at genetic similarity of 0.64. In the cluster I, five CSSLs identified as diverse genotypes had wild ancestor segments responsible for drought tolerance, and hence they could be utilized as potential donors. The popular Indian varieties, Swarna-sub1 and IR64-sub1, could be used as recurrent parents in the future breeding program for developing varieties for abiotic stresses such as submergence and drought.

关键词： genetic diversity marker-assisted selection microsatellite marker rice drought stress submergence chromosome segment substitution line

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Performance assessment of genetic programming(GP)and minimax probability machine regression(MPMR)for prediction of seismic ultrasonic attenuation

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Earthquake Science 2013年第2期26卷 147-150页

作者： manoj kumar Manav Mittal Pijush Samui National Institute of Rock Mechanics Kolar Gold Fields 563117 Karnataka India School of Mechanical and Building Science VIT University Centre for Disaster Mitigation and Management VIT University

The determination of seismic attenuation （s）（dB/cm） is a challenging task in earthquake science. This article employs genetic programming （GP） and minimax probability machine regression （MPMR） for prediction of s. GP is developed based on genetic algo- rithm. MPMR maximizes the minimum probability of future predictions being within some bound of the true regression function. Porosity （n）（%）, permeability （k）（millidarcy）, grain size （d）（μm）, and clay content （c）（%） have been considered as inputs of GP and MPMR. The output of GP and MPMR is s. The developed GP gives an equation for prediction of s. The results of GP and MPMR have been compared with the artificial neural net- work. This article gives robust models based on GP and MPMR for prediction of s.

关键词： Seismic attenuation Geneticprogramming Minimax probability machineregression Artificial neural network Prediction

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Circulatory and hepatic failure at admission predicts mortality of severe scrub typhus patients: A prospective cohort study

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Asian Pacific Journal of Tropical Medicine 2021年第5期14卷 209-213页

作者： Ashok kumar Pannu Atul Saroch Saurabh Chandrabhan Sharda manoj kumar Debnath Manisha Biswal Navneet Sharma Department of Internal Medicine Postgraduate Institute of Medical Education and ResearchChandigarhIndia Department of Medical Microbiology Postgraduate Institute of Medical Education and ResearchChandigarhIndia

Objective:To study the spectrum and outcome of severe scrub typhus in adult patients and to predict the hospital mortality by organ failure on ***:This was a prospective observational cohort study conducted between July 2017 and October 2020 at the medical emergency centre of PGIMER,Chandigarh,*** hundred and twenty-six patients aged≥13 years were diagnosed with scrub *** disease was defined as the presence of organ failure based on the Sequential Organ Failure Assessment(SOFA)score on ***:About two-thirds of the patients were from geographic regions outside the endemic sub-Himalayan ***(99.21%)and dyspnea(79.36%)were the most frequent *** failure(81.75%)was the most common organ failure,followed by hepatic(52.38%),coagulative(47.62%),circulatory(33.33%),renal(21.43%),and cerebral dysfunction(13.49%).The median(Q1,Q3)SOFA score was 8(6,9),and 48.41%of the patients had a quick-SOFA score≥*** supports with invasive ventilation(40.48%),vasopressors(36.51%),and renal replacement therapy(7.14%)were frequently *** in-hospital mortality was 11.90% and was independently predicted by circulatory and hepatic failures on multivariate logistic regression(OR 11.12,95%CI 1.73-71.31 and OR 8.49,95%CI 1.18-61.41,respectively).Conclusions:Most patients had pulmonary dysfunction;circulatory or hepatic failure on admission strongly predicts death.

关键词： Scrub typhus Orientia tsutsugamushi Organ failure Mortality Prognosis

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