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Crosstalk between Autophagy and Apoptosis in Intervertebral Disc Degeneration

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Journal of Biosciences and Medicines 2021年第12期9卷 15-29页

作者： Bruno Saciloto Natália Fontana Nicoletti Manuela Peletti-Figueiró Asdrubal falavigna Health Sciences Graduate Program University of Caxias do Sul (UCS) Caxias do Sul Brazil Cell Therapy Laboratory (LATEC) University of Caxias do Sul (UCS) Caxias do Sul Brazil

Objective: To describe the relationship between autophagy and apoptosis and the possible signaling pathways involved in degenerative lumbar intervertebral disc. Summary of Background Data: Autophagy and apoptosis are regulatory cellular mechanisms that determine many pathologies, including degenerative intervertebral disc disease. The interactions between these events in the damage or protection of intervertebral disc cells and in cellular homeostasis remain controversial. Methods: The sample size was twenty patients who underwent lumbar spine surgery for symptomatic disc herniation or spondylolisthesis. Intervertebral discs were classified by magnetic resonance as Pfirrmann grade IV and grade V. Six patients were operated on two levels, resulting in twenty-six intervertebral discs that were submitted to immunohistochemistry to verify the protein expression of autophagy and apoptosis markers. Results: The autophagic markers had greater protein expression in the human intervertebral disc (Pfirrmann Grades IV and V). Under these conditions, autophagy and apoptosis showed a negative correlation. Regarding apoptosis, caspase 8 presented the highest protein expression, which allows inferring the preference for the extrinsic pathway in cell death. Conclusions: Autophagy had the greatest protein expression negative profile compared to apoptosis. Caspase 8 had the highest protein expression in apoptosis.

关键词： Intervertebral Disc Degeneration Autophagy Apoptosis Crosstalk

Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development

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Horticulture Research 2016年第1期3卷 124-133页

作者： Danielle Costenaro da Silva Vítor da Silveira falavigna Marianna Fasoli Vanessa Buffon Diogo Denardi Porto Georgios Joannis Pappas Jr Mario Pezzotti Giancarlo Pasquali Luís Fernando Revers Graduate Program in Cell and Molecular Biology Center for BiotechnologyUniversidade Federal do Rio Grande do SulPorto Alegre91501-970RSBrazil Dipartimento di Biotecnologie Universitàdegli Studi di VeronaVerona37134Italy Embrapa Uva e Vinho Bento Gonçalves95701-008RSBrazil Embrapa Semiárido Petrolina56302-970PEBrazil Department of Cell Biology Universidade de BrasíliaBrasília70910-900DFBrazil Syngenta Seeds Ltda UberlândiaMG38407-049Brazil Modesto CA 95354USA

The Dof(DNA-binding with one finger)protein family spans a group of plant transcription factors involved in the regulation of several functions,such as plant responses to stress,hormones and light,phytochrome signaling and seed *** we describe the Dof-like gene family in grapevine(Vitis vinifera L.),which consists of 25 genes coding for *** extensive in silico characterization of the VviDofL gene family was ***,the expression of the entire gene family was assessed in 54 grapevine tissues and organs using an integrated approach with microarray(cv Corvina)and real-time PCR(cv Pinot Noir)*** phylogenetic analysis comparing grapevine sequences with those of Arabidopsis,tomato,poplar and already described Dof genes in other species allowed us to identify several duplicated *** diversification of grapevine DofL genes during evolution likely resulted in a broader range of biological ***,distinct expression patterns were identified between samples analyzed,corroborating such *** expression results indicate that several VviDofL genes perform their functional roles mainly during flower,berry and seed development,highlighting their importance for grapevine growth and *** identification of similar expression profiles between both approaches strongly suggests that these genes have important regulatory roles that are evolutionally conserved between grapevine cvs Corvina and Pinot Noir.

关键词： involvement organs assessed

Genotoxicity Clues to Predict Intervertebral Disc Degeneration: A Systematic Review

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Journal of Biosciences and Medicines 2020年第12期8卷 68-77页

作者： Charles André Carazzo Bruno Saciloto Manuela Peletti Figueiró Natalia Fontana Nicoletti Asdrubal falavigna Health Sciences postgraduate Program University of Caxias do Sul (UCS) Caxias do Sul Brazil Cell Therapy Laboratory (LATEC) University of Caxias do Sul (UCS) Caxias do Sul Brazil

Objective: To characterize the association between DNA damage and Intervertebral disc degeneration (IDD). Summary of Background Data: IDD is the main disorder causing low back pain and is the most promising target for intervention. Many factors can contribute to the etiology, such as genetics, environment and lifestyle, but it is not yet fully understood. DNA damage can influence this process and needs to be studied, as well as the agents that can determine these damages. Methods: A systematic literature search of PubMed, Web of Science and Scopus was performed to identify studies related to DNA damage to the intervertebral disc. Results: After screening 61 records, 7 articles were included according to the selection criteria. All studies showed some relation between DNA damage and IDD. However, DNA damage was always considered a secondary issue to be investigated. Conclusions: Many factors can influence DNA damage induced by different genotoxic agents on the degenerative cascade of IVD. However, the correlation between IDD severity and DNA damage, as well as the factual role of DNA damage in disc degeneration could not be defined.

关键词： DNA Damage Genotoxicity Biomarker Intervertebral Disc Degeneration

Towards on the Mediators in Collagen Replacement: Effect of the Active Molecules into a Polymeric Biomaterial

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Journal of Biosciences and Medicines 2021年第6期9卷 26-42页

作者： Anderson Ricardo Ingracio Natália Fontana Nicoletti Fernanda Trindade Gonzalez Dias Manuela Peletti-Figueiró Otávio Bianchi Asdrubal falavigna Health Sciences Graduate Program Universidade de Caxias do Sul (UCS) Caxias do Sul RS Brazil Cell Therapy Laboratory (LATEC) Universidade de Caxias do Sul (UCS) Caxias do Sul RS Brazil Materials Science Graduate Program (PGMAT) Universidade de Caxias do Sul (UCS) Caxias do Sul RS Brazil

Modulating healing factors could avoid or minimize some possible pathological processes in collagen deposition. The present study was aimed to evaluated the role of active biomolecules such as PDGF-BB and PRP loaded or not into polymeric biomaterial to seek potential mediators in types I and III collagen deposition and epithelization. The healing phases were investigated by using an in vivo full-thickness wound rat model. At zero, 3rd, 7th and 14th days after the experimental model, the size of the wound areas was photographed. The nanofibrous materials were biocompatible and did not cause any local adverse reaction and/or inflammation. On day 14 the wounds had healed almost 100% with better signs of healing, however there was no obvious difference in the wound contraction rates. At the end of 14 days, samples from the center of the lesion were collected when histological features and immunopositivity for collagen I and III expressions were assessed. There was no significant difference in the epithelization among the groups. Wounds treated with PRP and with PA-6/SOMA plus PDGF-BB had significantly lower amounts of type III collagen. The amounts of type I collagen did not have a statistically different deposition among the experimental groups. The association of PDGF-BB with PA-6/SOMA emerges as an alternative for topical application to unfavorable anatomical sites, suggesting that these associations may have a positive modulation on the process of accelerated healing remodeling.

关键词： Collagen Fibrosis Platelet-Rich Plasma Polyamide-6 and Biomaterial