Crosstalk between Autophagy and Apoptosis in Intervertebral Disc Degeneration
Crosstalk between Autophagy and Apoptosis in Intervertebral Disc Degeneration作者机构：Health Sciences Graduate Program University of Caxias do Sul (UCS) Caxias do Sul Brazil Cell Therapy Laboratory (LATEC) University of Caxias do Sul (UCS) Caxias do Sul Brazil
出 版 物：《Journal of Biosciences and Medicines》 (生物科学与医学（英文）)
年 卷 期：2021年第9卷第12期
页 面：15-29页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Intervertebral Disc Degeneration Autophagy Apoptosis Crosstalk
摘 要：Objective: To describe the relationship between autophagy and apoptosis and the possible signaling pathways involved in degenerative lumbar intervertebral disc. Summary of Background Data: Autophagy and apoptosis are regulatory cellular mechanisms that determine many pathologies, including degenerative intervertebral disc disease. The interactions between these events in the damage or protection of intervertebral disc cells and in cellular homeostasis remain controversial. Methods: The sample size was twenty patients who underwent lumbar spine surgery for symptomatic disc herniation or spondylolisthesis. Intervertebral discs were classified by magnetic resonance as Pfirrmann grade IV and grade V. Six patients were operated on two levels, resulting in twenty-six intervertebral discs that were submitted to immunohistochemistry to verify the protein expression of autophagy and apoptosis markers. Results: The autophagic markers had greater protein expression in the human intervertebral disc (Pfirrmann Grades IV and V). Under these conditions, autophagy and apoptosis showed a negative correlation. Regarding apoptosis, caspase 8 presented the highest protein expression, which allows inferring the preference for the extrinsic pathway in cell death. Conclusions: Autophagy had the greatest protein expression negative profile compared to apoptosis. Caspase 8 had the highest protein expression in apoptosis.
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