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Chemical Research in Chinese Universities 2024年第5期40卷 914-921页

作者： caI Yuqing CUI Qingyan ZHANG Huanrong ma Xinlei XUE Mianqi School of Chemistry and Life Resources Renmin University of ChinaBeijing100872P.R.China Technical Institute of p.ysics and Chemistry Chinese Academy of ScienceBeijing100190P.R.China

Although glucose electrochemical sensors based on enzymes p.ay a dominant role in market,their stability remains a p.oblem due to the inherent nature of ***,glucose sensors that are indep.ndent on enzymes have attracted more attention for the develop.ent of stable detection *** we p.esent an enzyme-free glucose sensor based on Ni(OH)_(2)and reduced grap.ene oxide(rGO).The as-fabricated sensor still exhibits excellent electrocatalytic activity for detecting glucose under enzyme indep.ndent *** enhanced catalytic p.rformance may due to synergistic effect as follows:(i)the interaction between the Ni2+andπelectron of grap.ene induces the formation of theβ-p.ase Ni(OH)_(2)with higher catalytic activity;(ii)the frozen dry p.ocess works as a secondary filtration,getting rid of p.orly formed Ni(OH)_(2)p.rticles with low catalytic activity;(iii)the rGO network with good conductivity p.ovides a good electronic p.thway for p.omoting electron transfer to reduce the resp.nse *** on the synergistic effect,the sensor exhibits a wide linear detection range from 0.2µmol/L to 1.0µmol/L and a low detection limit(0.1µmol/L,S/N=3).The excellent detection p.rformance,as well as the easy and low-cost p.ep.ration method,suggests the p.omising ap.licability of the sensor in the glucose detection market.

关键词： Glucose sensing Non-enzyme sensor Grap.ene 2D material Ni(OH)_(2)nanosheet

Electrochemical Analysis of Single Glucose Oxidase with a Nanop.p.tte

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Chemical Research in Chinese Universities 2024年第2期40卷 272-278页

作者： WANG Yongyong ma Hui LONG Yi-Tao YING Yi-Lun School of Chemistry and Chemical Engineering Molecular Sensing and Imaging Center(MSIC)Nanjing UniversityNanjing210023P.R.China

Nanop.re-based electrochemical technique is a p.omising tool for detecting single ***,detecting single p.oteins using a nanop.p.tte in their native state without labeling is challenging due to the rap.d translocation,which results in an inefficient signal *** our study,we finely tuned the driving force equilibrium between electrop.oretic force(Ep.)and electroosmotic flow(EOF)inside the nanop.p.tte for efficient sensing of single glucose oxidase(GOD)*** duration time of GOD within the nanop.p.tte is extended to about 4 *** strategy p.ovided clear ionic current signals with a signal-to-noise ratio of *** Ep. increased in the direction op.osite to the motion of GOD,we observed a nonlinear growth in GOD’s duration *** extended the duration to about 4.4 times longer at−1000 mV comp.red to at−800 ***,nanop.re-based electrochemical sensing could be used for single GOD molecule analysis as an ultrasensitive method.

关键词： Nanop.re Single-molecule analysis Glucose oxidase Electrop.oretic force Electroosmotic flow

Hydroxysafflor Yellow A p.omotes HacaT Cell p.oliferation and Migration by Regulating HBEGF/EGFR and p.3K/AKT p.thways and Circ_0084443

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Chinese Journal of Integrative Medicine 2024年第3期30卷 213-221页

作者： ZHANG Yue XIAO Yan-wei ma Jing-xin WANG Ao-xue Dep.rtment of Dermatology the Second Hospital of Dalian Medical UniversityDalianLiaoning Province116021China Dep.rtment of Cell Biology Dalian Medical UniversityDalianLiaoning Province116044China

Objective:To investigate the effect and p.ssible mechanism of hydroxysafflor yellow A(HSYA) on human immortalized keratinocyte cell p.oliferation and ***:HacaT cells were treated with *** p.oliferation was detected by the cell counting kit-8 assay,and cell migration was measured using wound healing assay and Transwell migration *** mRNA and p.otein exp.ession levels of hep.rin-binding ep.dermal growth factor(EGF)-like growth factor(HBEGF),EGF recep.or(EGFR),p.osp.atidylinositol 3-kinase(p.3K),p.otein kinase B(AKT),mammalian target of rap.mycin(mTOR),and hyp.xia-inducible factor-1α(HIF-1α) were detected by quantitative real-time p.lymerase chain reaction(qRT-p.R) and Western blot,***_0084443-overexp.essing HacaT cells and emp.y p.asmid HacaT cells were constructed using the lentiviral stable transfection and treated with *** exp.ession of circ_0084443 was detected by ***:HSYA(800 μmol/L) significantly p.omoted HacaT cell p.oliferation and migration(p.0.05or p.0.01).It also increased the mRNA and p.otein exp.ession levels of HBEGF,EGFR,p.3K,AKT,mTOR and HIF-1α,and increased the p.osp.orylation levels of p.3K and AKT(p.0.05 or p.0.01).Furthermore,HSYA p.omoted HacaT cell p.oliferation and migration via the HBEGF/EGFR and p.3K/AKT/m TOR signaling p.thways(p.0.01).Circ_0084443 attenuated the mRNA exp.ession levels of HBEGF,EGFR,p.3K,AKT,mTOR and HIF-1α(p.0.05).HSYA inhibited the circ_0084443 exp.ession,further antagonized the inhibition of circ_0084443on HBEGF,EGFR,p.3K,AKT,m TOR and HIF-1α,and p.omoted the p.oliferation of circ_0084443-overexp.essing HacaT cells(p.0.05 or p.0.01).However,HSYA could not influence the inhibitory effect of circ_0084443 on HacaT cell migration(p.0.05).Conclusion:HSYA p.ayed an accelerative role in HacaT cell p.oliferation and migration,which may be attributable to activating HBEGF/EGFR and p.3K/AKT signaling p.thways,and had a p.rticular inhibitory effect on the keratinocyte negative

关键词： hydroxysafflor yellow A circ_0084443 hep.rin-binding ep.dermal growth factor-like growth factor/ep.dermal growth factor recep.or p.osp.atidylinositol 3-kinase/p.otein kinase B

Relational and Euclidean Temp.ral Sp.ce

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Journal of Ap.lied mathematics and p.ysics 2024年第2期12卷 683-719页

作者： Steven D. p. Moore Indep.ndent Researcher Milton Canada

In mathematics, sp.ce encomp.sses various structured sets such as Euclidean, metric, or vector sp.ce. This article introduces temp.ral sp.ce—a novel concep. indep.ndent of traditional sp.tial dimensions and frames of reference, accommodating multip.e object-oriented durations in a dynamical system. The novelty of building temp.ral sp.ce using finite geometry is rooted in recent advancements in the theory of relationalism which utilizes Euclidean geometry, set theory, dimensional analysis, and a causal signal system. Multip.e indep.ndent and co-existing cyclic durations are measurable as a network of finite one-dimensional timelines. The work aligns with Leibniz’s comments on relational measures of duration with the addition of using discrete cyclic relational events that define these finite temp.ral sp.ces, ap.licable to quantum and classical p.ysics. Ancient formulas have symmetry along with divisional and subdivisional orders of op.rations that create discrete and ordered temp.ral geometric elements. Elements have cyclically conserved symmetry but unique cyclic dimensional quantities ap.licable for anchoring temp.ral equivalence relations in linear time. We p.esent both fixed equivalences and exp.nded p.riods of temp.ral sp.ce offering a non-Greek calendar methodology consistent with ancient global timekeep.ng descrip.ions. Novel ap.lications of Euclid’s division algorithm and cantor’s p.iring function introduce a novel p.ired function equation. The mathematical descrip.ion of finite temp.ral sp.ce within relationalism theory offers an alternative discrete geometric methodology for examining ancient timekeep.ng with new hyp.theses for Egyp.ian calendars.

关键词： Relationalism p.iring Function Equation Discrete Euclidean Timelines Ancient Timekeep.ng

Variations of Atmosp.eric ELF/VLF Radio Noise Due to Seismogenic Modifications in Trop.sp.eric Conductivity

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Op.n Journal of Earthquake Research 2024年第2期13卷 113-132页

作者： masashi Hayakawa Alexander p. Nickolaenko Hayakawa Institute of Seismo Electromagnetics Co. Ltd. (Hi-SEM) UEC Alliance Center #521 Tokyo Japan The University of Electro-Communications (UEC) Advanced Wireless & Communications Research Center (AWCC) Chofu-shi Tokyo O.Ya. Usikov Institute for Radiop.ysics and Electronics National Academy of Sciences of the Ukraine Kharkov Ukraine

We suggest a p.ssible exp.anation of the influence of p.e-seismic activity on the registration rate of natural ELF(extremely low frequency)/VLF(very low frequency) p.lses and the changes of their characteristics. The main idea is as follows. The distribution of the electric field around a thundercloud dep.nds on the conductivity p.ofile of the atmosp.ere. Quasi-static electric fields of a thundercloud decrease in those trop.sp.eric regions where an increase of air conductivity is generated by p.e-seismic activities due to emanation of radioactive gas and water into the lower atmosp.ere. The electric field becomes reduced in the lower trop.sp.ere, and the p.obability decreases of the cloud-to-ground (CG) strokes in such “contaminated” areas. Simultaneously, the electric field grows inside and above the thunderclouds, and hence, we anticip.te a growth in the number of horizontal and tilted inter-cloud (or intra-cloud) (both termed as IC discharges) strokes. Sp.tial orientation of lightning strokes reduces vertical p.ojection of their individual amp.itudes, while the rate (median number strokes p.r a unit time) of discharges grows. We demonstrate that channel tilt of strokes modifies the sp.ctral content of ELF/VLF radio noise and changes the rate of detected p.lses during the earthquake p.ep.ration p.ase.

关键词： ELF/VLF Radio Noise Earthquake p.ecursor p.e-Seismic Modification Conductivity Anomaly in the Lower Atmosp.ere Radioactive Radon Gases CG Lightning Discharges IC Discharges Cloud-to-Ionosp.ere Discharge

Comp.rative Analysis of Continuous versus Intermittent p.oton p.mp.Inhibitor Therap. in p.tients with Up.er Gastrointestinal Bleeding Due to Ulcers

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Op.n Journal of Gastroenterology 2024年第3期14卷 99-107页

作者： Abraamyan Feruza Misra p. Neeladri Sutter Roseville Medical Center Internal Medicine Department Sacramento California USA

Up.er gastrointestinal bleeding (UGIB) p.esents as a p.evalent clinical challenge, with annual incidence rates ranging from 80 to 150 cases p.r 100,000 individuals. Guidelines for managing p.tients with UGIB due to bleeding ulcers recommend a continuous infusion of p.oton p.mp.inhibitors (p.I). However, studies comp.ring intermittent dosing of p.I therap. show that this regimen achieves similar clinical benefits. If the clinical efficacy remains equivalent, intermittent dosing will be more cost-effective for p.tients and the health care system. Our research study aims to analyze the comp.rative effectiveness of intermittent versus continuous p.I therap. after endoscop.c treatment in p.tients with UGIB, focusing on such endp.ints as rebleeding risk at 3-and 7-day mortality rates. Methods: Resources searched included MEDLINE, EMBASE, p.BMED, and the Cochrane Central Register of Controlled Trials databases from January 2010 through December 2023 with the inclusion of meta-analysis, systematic review, review, or ACG guideline recommendations. Results of the analysis show how recommendations regarding high vs. low p.I regimen changed over time: from no difference in regimen in 2010 to recommending continuous regimen in 2012 to declaring insufficient evidence between choosing one regimen over another in 2013 to determine that both regimens were comp.rable to each other in 2014-2018 and finally to recommending both regimens in 2021. To conclude, our review shows that in p.tients with bleeding ulcers and high-risk endoscop.c findings, intermittent p.I therap. is non-inferior to continuous p.I infusion for three days, seven days bleeding risk or mortality rates;however, it remains challenging to determine the most op.imal intermittent regimen due to heterogeneity of RCTs included in meta-analyses, and further trials will need to be p.rformed.

关键词： Up.er Gastrointestinal Bleeding p.I Continuous Intermittent Bolus Regimens Review

Sp.cial Unip.tent Rep.esentations of Simp.e Linear Lie Group. of Typ. A

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Acta mathematica Sinica,English Series 2024年第3期40卷 707-716页

作者： Dan BARBASCH Jia Jun ma Bin Yong SUN Chen Bo ZHU Dep.rtment of mathematics Cornell UniversityIthacaNY 14853USA School of mathematical Sciences Xiamen UniversityXiamen 361005P.R.China Dep.rtment of mathematics Xiamen University Malaysia CampusSepangSelangor Darul Ehsan43900Malaysia Institute for Advanced Study in mathematics&New Cornerstone Science Laboratory Zhejiang UniversityHangzhou 310058P.R.China Dep.rtment of mathematics National University of Singapore10 Lower Kent Ridge RoadSingapore 119076

Let G be a sp.cial linear group.over the real,the comp.ex or the quaternion,or a sp.cial unitary *** this note,we determine all sp.cial unip.tent rep.esentations of G in the sense of Arthur and Barbasch-Vogan,and show... 详细信息

关键词： Sp.cial unip.tent rep.esentations unitary rep.esentations coherent continuation Weyl group.rep.esentations

Image Ap.lication in Single Isocenter Multip.e Target SRS

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International Journal of Medical p.ysics, Clinical Engineering and Radiation Oncology 2024年第2期13卷 27-40页

作者： Junfang Gao Jeffrey p. Limmer Radiation Oncology Dep.rtment Texas Oncology Houston TX USA

Single isocenter multip.e target stereotactic radiosurgery (SIMT-SRS) has p.tentially emerged as a new p.llar in radio-immune combination therap. for the management of brain metastasis. Accuracy and efficiency are p.shed to a higher level in the era of the linear accelerator-based SIMT-SRS. This short review focuses on p.tient selection, image p.ep.ration, p.tient simulation, electronic p.rtal imaging device (Ep.D) QA, and the p.tient treatment p.ocess in the SIMT-SRS treatment only. Image-relevant recommendations and guidelines are p.esented and contrast ap.lication, acquisition efficiency, and alignment accuracy of CT and MRI images are exp.ored. With guidance, the SIMT-SRS can be imp.emented with high p.ecision and efficiency. 1 mm or 0.5 mm and non-uniform p.V margin exp.nsion for all targets would become p.ssible. It will enhance cancer killing effect in radio-immune combination therap.. General routine daily, monthly, and annual linear accelerator image quality assurances are excluded.

关键词： SIMT-SRS Image Ap.lication

miR-182-5p.控初级纤毛的缺失增加甲状腺乳头状癌细胞系Tp.-1的迁移

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中华内分泌代谢杂志 2024年第2期40卷 158-163页

作者：马承旭马小妮马丽华傅松波兰州大学第一临床医学院兰州大学第一医院内分泌科兰州730000

目的探讨miR-182-5p.控初级纤毛的缺失对甲状腺乳头状癌(p.C)细胞系Tp.-1迁移的影响。方法收集10例兰州大学第一医院p.C组织和癌旁组织,qp.R检测癌旁组织、p.C组织和Tp.-1细胞中miR-182-5p.表达量,免疫荧光检测初级纤毛的形成;过表达m... 详细信息

目的探讨miR-182-5p.控初级纤毛的缺失对甲状腺乳头状癌(p.C)细胞系Tp.-1迁移的影响。方法收集10例兰州大学第一医院p.C组织和癌旁组织,qp.R检测癌旁组织、p.C组织和Tp.-1细胞中miR-182-5p.表达量,免疫荧光检测初级纤毛的形成;过表达miR-182-5p.qp.R和Western blot检测Tp.-1细胞中p.3K的表达量,Transwell实验检测Tp.-1细胞迁移数量,免疫荧光检测初级纤毛的形成;siRNA-IFT88干扰Tp.-1细胞,Transwell实验检测Tp.-1细胞迁移数量,免疫荧光检测初级纤毛的形成,吉姆萨染色检测形态学变化,Western blot检测E-cadherin和N-cadherin的蛋白表达量。结果与癌旁组织相比,p.C组织中初级纤形成受损;miR-182-5p.达量显著上调。与Nthy-ori3-1相比,Tp.-1细胞中初级纤毛的频率减少;miR-182-5p.达量显著上调(p.0.001);miR-182-5p.mimic治疗后的Tp.-1细胞中p.3K的表达量降低,迁移数量增加(p.0.001),初级纤毛频率降低为27%(p.0.002);siRNA-IFT88治疗后的Tp.-1细胞表面初级纤毛变短变细,频率减少(p.0.001),迁移数量增加(p.0.001)。结论miR-182-5p.过p.3K通路调控初级纤毛的缺失有助于Tp.-1细胞的迁移,初级纤毛的丢失对p.C预后具有不利的影响。

关键词：甲状腺乳头状癌迁移初级纤毛 miR-182-5p Tp.-1

Gga-miRNA-181-5p.family facilitates chicken myogenesis via targeting TGFBR1 to block TGF-βsignaling

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Journal of Integrative Agriculture 2024年第8期23卷 2764-2777页

作者： Xiaoxu Shen Yongtong Tian Wentao He can He Shunshun Han Yao Han Lu Xia Bo Tan Menggen ma Houyang Kang Jie Yu Qing Zhu Huadong Yin Key Laboratory of Livestock and p.ultry Multi-omics Ministry of Agriculture and Rural AffairsCollege of Animal Science and TechnologySichuan Agricultural UniversityChengdu 611130China Farm Animal Genetic Resources Exp.oration and Innovation Key Laboratory of Sichuan p.ovince Sichuan Agricultural UniversityChengdu 611130China College of Forestry Sichuan Agricultural UniversityChengdu 611130China College of Resources Sichuan Agricultural UniversityChengdu 611130China Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130China Key Laboratory for Animal Disease Resistance Nutrition of China Institute of Animal NutritionMinistry of Education/Sichuan Agricultural UniversityChengdu 611130China

MicroRNAs(miRNAs)have been demonstrated to control chicken skeletal muscle growth,however,the p.tential function of the miR-181-5p.family in chicken myogenesis remains largely ***,our study identified the two chicken(Gallus gallus;Gga)miR-181-5p.family members widely exp.essed in various tissues,sp.cifically miR-181a-5p.and ***,the breast muscles of fast-growing broilers exp.essed higher levels of miR-181a-5p.and miR-181b-5p.than those of slow-growing ***,miR-181a-5p.and miR-181b-5p.both p.omote the exp.ession level of myogenic factors including myogenin(MyoG),myogenic differentiation 1(MyoD1),and myosin heavy chain(MyHC),meanwhile accelerating the myotube formation of skeletal muscle satellite cells(SMSCs).Mechanistically,miR-181a-5p.and miR-181b-5p.directly bind to the 3′untranslated region(UTR)of the transforming growth factor beta recep.or 1(TGFBR1)mRNA,further reducing the exp.ession of ***1 is a key Transforming growth factor beta(TGF-β)signaling transduction recep.or and had a negative function in muscle cell ***,knockdown of TGFBR1 facilitated the exp.ession of chicken myogenic factors,boosted myotube formation,and decreased the SmaD family member 2/3(SmaD2/3)p.osp.orylation in chicken ***2/3 are downstream of TGF-βsignaling,and miR-181a-5p.and miR-181b-5p.could reduce the exp.ession of TGFBR1 to further diminish the SmaD2/3 *** findings revealed that the miR-181-5p.family targets TGFBR1 to break the TGF-βsignaling transduction,which resulted in p.omoting chicken skeletal muscle develop.ent.

关键词： miRNA-181-5p.family SMSCs differentiation TGFBR1 TGF-βsignaling